Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts
- Autores
- Vázquez, Antonio A.; Sabourin, Emeline; Alda, Maria del Pilar; Leroy, Clémentine; Leray, Carole; Carron, Eric; Mulero, Stephen; Caty, Céline; Hasfia, Sarah; Boisseau, Michel; Saugné, Lucas; Pineau, Olivier; Blanchon, Thomas; Alba, Annia; Faugère, Dominique; Vittecoq, Marion; Hurtrez Boussès, Sylvie
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fasciolosis is a worldwide spread parasitosis mainly caused by the trematode Fasciola hepatica. This disease is particularly important for public health in tropical regions, but it can also affect the economies of many developed countries due to large infections in domestic animals. Although several studies have tried to understand the transmission by studying the prevalence of different host species, only a few have used population genetic approaches to understand the links between domestic and wildlife infections. Here, we present the results of such genetic approach combined with classical parasitological data (prevalence and intensity) by studying domestic and wild definitive hosts from Camargue (southern France) where fasciolosis is considered as a problem. We found 60% of domestic hosts (cattle) infected with F. hepatica but lower values in wild hosts (nutria, 19%; wild boars, 4.5%). We explored nine variable microsatellite loci for 1,148 adult flukes recovered from four different populations (non-treated cattle, treated cattle, nutria and wild boars). Populations from the four groups differed, though we found a number of migrants particularly non-treated cattle and nutria. Overall, we detected 729 different multilocus genotypes (from 783 completely genotyped individuals) and only 46 genotypes repeated across samples. Finally, we experimentally infected native and introduced intermediate snail hosts to explore their compatibility with F. hepatica and assess the risks of fasciolosis expansion in the region. The introduced species Galba truncatula and Pseudosuccinea columella attained the higher values of overall compatibility in relation to the European species. However, concerning the origin, sympatric combinations of G. truncatula were more compatible (higher prevalence, intensity and survival) than the allopatric tested. According to our results, we should note that the assessment of epidemiological risks cannot be limited to a single host–parasite system, but should focus on understanding the diversity of hosts in the heterogeneous environment through space and time.
Fil: Vázquez, Antonio A.. Instituto de Medicina Tropical “Pedro Kourí”; Cuba. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia
Fil: Sabourin, Emeline. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Alda, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Zoología de Invertebrados I; Argentina. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Leroy, Clémentine. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Leray, Carole. Institut de Recherche de la Tour du Valat; Francia
Fil: Carron, Eric. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Mulero, Stephen. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia. Université de Perpignan Via Domitia; Francia
Fil: Caty, Céline. Institut de Recherche de la Tour du Valat; Francia
Fil: Hasfia, Sarah. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Boisseau, Michel. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Saugné, Lucas. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Pineau, Olivier. Institut de Recherche de la Tour du Valat; Francia
Fil: Blanchon, Thomas. Institut de Recherche de la Tour du Valat; Francia
Fil: Alba, Annia. Instituto de Medicina Tropical “Pedro Kourí”; Cuba. Università di Corsica Pasquale Paoli; Francia
Fil: Faugère, Dominique. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia
Fil: Vittecoq, Marion. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia. Institut de Recherche de la Tour du Valat; Francia
Fil: Hurtrez Boussès, Sylvie. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia - Materia
-
DOMESTIC AND WILDLIFE INFECTION
FASCIOLOSIS
LYMNAEID SNAILS
POPULATION GENETICS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/131516
Ver los metadatos del registro completo
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Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hostsVázquez, Antonio A.Sabourin, EmelineAlda, Maria del PilarLeroy, ClémentineLeray, CaroleCarron, EricMulero, StephenCaty, CélineHasfia, SarahBoisseau, MichelSaugné, LucasPineau, OlivierBlanchon, ThomasAlba, AnniaFaugère, DominiqueVittecoq, MarionHurtrez Boussès, SylvieDOMESTIC AND WILDLIFE INFECTIONFASCIOLOSISLYMNAEID SNAILSPOPULATION GENETICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fasciolosis is a worldwide spread parasitosis mainly caused by the trematode Fasciola hepatica. This disease is particularly important for public health in tropical regions, but it can also affect the economies of many developed countries due to large infections in domestic animals. Although several studies have tried to understand the transmission by studying the prevalence of different host species, only a few have used population genetic approaches to understand the links between domestic and wildlife infections. Here, we present the results of such genetic approach combined with classical parasitological data (prevalence and intensity) by studying domestic and wild definitive hosts from Camargue (southern France) where fasciolosis is considered as a problem. We found 60% of domestic hosts (cattle) infected with F. hepatica but lower values in wild hosts (nutria, 19%; wild boars, 4.5%). We explored nine variable microsatellite loci for 1,148 adult flukes recovered from four different populations (non-treated cattle, treated cattle, nutria and wild boars). Populations from the four groups differed, though we found a number of migrants particularly non-treated cattle and nutria. Overall, we detected 729 different multilocus genotypes (from 783 completely genotyped individuals) and only 46 genotypes repeated across samples. Finally, we experimentally infected native and introduced intermediate snail hosts to explore their compatibility with F. hepatica and assess the risks of fasciolosis expansion in the region. The introduced species Galba truncatula and Pseudosuccinea columella attained the higher values of overall compatibility in relation to the European species. However, concerning the origin, sympatric combinations of G. truncatula were more compatible (higher prevalence, intensity and survival) than the allopatric tested. According to our results, we should note that the assessment of epidemiological risks cannot be limited to a single host–parasite system, but should focus on understanding the diversity of hosts in the heterogeneous environment through space and time.Fil: Vázquez, Antonio A.. Instituto de Medicina Tropical “Pedro Kourí”; Cuba. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; FranciaFil: Sabourin, Emeline. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; FranciaFil: Alda, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Zoología de Invertebrados I; Argentina. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; FranciaFil: Leroy, Clémentine. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; FranciaFil: Leray, Carole. Institut de Recherche de la Tour du Valat; FranciaFil: Carron, Eric. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; FranciaFil: Mulero, Stephen. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia. Université de Perpignan Via Domitia; FranciaFil: Caty, Céline. Institut de Recherche de la Tour du Valat; FranciaFil: Hasfia, Sarah. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; FranciaFil: Boisseau, Michel. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; FranciaFil: Saugné, Lucas. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; FranciaFil: Pineau, Olivier. Institut de Recherche de la Tour du Valat; FranciaFil: Blanchon, Thomas. Institut de Recherche de la Tour du Valat; FranciaFil: Alba, Annia. Instituto de Medicina Tropical “Pedro Kourí”; Cuba. Università di Corsica Pasquale Paoli; FranciaFil: Faugère, Dominique. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; FranciaFil: Vittecoq, Marion. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia. Institut de Recherche de la Tour du Valat; FranciaFil: Hurtrez Boussès, Sylvie. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; FranciaWiley Blackwell Publishing, Inc2020-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131516Vázquez, Antonio A.; Sabourin, Emeline; Alda, Maria del Pilar; Leroy, Clémentine; Leray, Carole; et al.; Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts; Wiley Blackwell Publishing, Inc; Transboundary And Emerging Diseases; 2020; 30-10-2020; 1-351865-16741865-1682CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/tbed.13882info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/tbed.13882info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:49Zoai:ri.conicet.gov.ar:11336/131516instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:49.868CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts |
| title |
Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts |
| spellingShingle |
Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts Vázquez, Antonio A. DOMESTIC AND WILDLIFE INFECTION FASCIOLOSIS LYMNAEID SNAILS POPULATION GENETICS |
| title_short |
Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts |
| title_full |
Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts |
| title_fullStr |
Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts |
| title_full_unstemmed |
Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts |
| title_sort |
Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts |
| dc.creator.none.fl_str_mv |
Vázquez, Antonio A. Sabourin, Emeline Alda, Maria del Pilar Leroy, Clémentine Leray, Carole Carron, Eric Mulero, Stephen Caty, Céline Hasfia, Sarah Boisseau, Michel Saugné, Lucas Pineau, Olivier Blanchon, Thomas Alba, Annia Faugère, Dominique Vittecoq, Marion Hurtrez Boussès, Sylvie |
| author |
Vázquez, Antonio A. |
| author_facet |
Vázquez, Antonio A. Sabourin, Emeline Alda, Maria del Pilar Leroy, Clémentine Leray, Carole Carron, Eric Mulero, Stephen Caty, Céline Hasfia, Sarah Boisseau, Michel Saugné, Lucas Pineau, Olivier Blanchon, Thomas Alba, Annia Faugère, Dominique Vittecoq, Marion Hurtrez Boussès, Sylvie |
| author_role |
author |
| author2 |
Sabourin, Emeline Alda, Maria del Pilar Leroy, Clémentine Leray, Carole Carron, Eric Mulero, Stephen Caty, Céline Hasfia, Sarah Boisseau, Michel Saugné, Lucas Pineau, Olivier Blanchon, Thomas Alba, Annia Faugère, Dominique Vittecoq, Marion Hurtrez Boussès, Sylvie |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
DOMESTIC AND WILDLIFE INFECTION FASCIOLOSIS LYMNAEID SNAILS POPULATION GENETICS |
| topic |
DOMESTIC AND WILDLIFE INFECTION FASCIOLOSIS LYMNAEID SNAILS POPULATION GENETICS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Fasciolosis is a worldwide spread parasitosis mainly caused by the trematode Fasciola hepatica. This disease is particularly important for public health in tropical regions, but it can also affect the economies of many developed countries due to large infections in domestic animals. Although several studies have tried to understand the transmission by studying the prevalence of different host species, only a few have used population genetic approaches to understand the links between domestic and wildlife infections. Here, we present the results of such genetic approach combined with classical parasitological data (prevalence and intensity) by studying domestic and wild definitive hosts from Camargue (southern France) where fasciolosis is considered as a problem. We found 60% of domestic hosts (cattle) infected with F. hepatica but lower values in wild hosts (nutria, 19%; wild boars, 4.5%). We explored nine variable microsatellite loci for 1,148 adult flukes recovered from four different populations (non-treated cattle, treated cattle, nutria and wild boars). Populations from the four groups differed, though we found a number of migrants particularly non-treated cattle and nutria. Overall, we detected 729 different multilocus genotypes (from 783 completely genotyped individuals) and only 46 genotypes repeated across samples. Finally, we experimentally infected native and introduced intermediate snail hosts to explore their compatibility with F. hepatica and assess the risks of fasciolosis expansion in the region. The introduced species Galba truncatula and Pseudosuccinea columella attained the higher values of overall compatibility in relation to the European species. However, concerning the origin, sympatric combinations of G. truncatula were more compatible (higher prevalence, intensity and survival) than the allopatric tested. According to our results, we should note that the assessment of epidemiological risks cannot be limited to a single host–parasite system, but should focus on understanding the diversity of hosts in the heterogeneous environment through space and time. Fil: Vázquez, Antonio A.. Instituto de Medicina Tropical “Pedro Kourí”; Cuba. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia Fil: Sabourin, Emeline. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia Fil: Alda, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Zoología de Invertebrados I; Argentina. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia Fil: Leroy, Clémentine. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia Fil: Leray, Carole. Institut de Recherche de la Tour du Valat; Francia Fil: Carron, Eric. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia Fil: Mulero, Stephen. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia. Université de Perpignan Via Domitia; Francia Fil: Caty, Céline. Institut de Recherche de la Tour du Valat; Francia Fil: Hasfia, Sarah. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia Fil: Boisseau, Michel. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia Fil: Saugné, Lucas. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia Fil: Pineau, Olivier. Institut de Recherche de la Tour du Valat; Francia Fil: Blanchon, Thomas. Institut de Recherche de la Tour du Valat; Francia Fil: Alba, Annia. Instituto de Medicina Tropical “Pedro Kourí”; Cuba. Università di Corsica Pasquale Paoli; Francia Fil: Faugère, Dominique. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia Fil: Vittecoq, Marion. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia. Institut de Recherche de la Tour du Valat; Francia Fil: Hurtrez Boussès, Sylvie. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia |
| description |
Fasciolosis is a worldwide spread parasitosis mainly caused by the trematode Fasciola hepatica. This disease is particularly important for public health in tropical regions, but it can also affect the economies of many developed countries due to large infections in domestic animals. Although several studies have tried to understand the transmission by studying the prevalence of different host species, only a few have used population genetic approaches to understand the links between domestic and wildlife infections. Here, we present the results of such genetic approach combined with classical parasitological data (prevalence and intensity) by studying domestic and wild definitive hosts from Camargue (southern France) where fasciolosis is considered as a problem. We found 60% of domestic hosts (cattle) infected with F. hepatica but lower values in wild hosts (nutria, 19%; wild boars, 4.5%). We explored nine variable microsatellite loci for 1,148 adult flukes recovered from four different populations (non-treated cattle, treated cattle, nutria and wild boars). Populations from the four groups differed, though we found a number of migrants particularly non-treated cattle and nutria. Overall, we detected 729 different multilocus genotypes (from 783 completely genotyped individuals) and only 46 genotypes repeated across samples. Finally, we experimentally infected native and introduced intermediate snail hosts to explore their compatibility with F. hepatica and assess the risks of fasciolosis expansion in the region. The introduced species Galba truncatula and Pseudosuccinea columella attained the higher values of overall compatibility in relation to the European species. However, concerning the origin, sympatric combinations of G. truncatula were more compatible (higher prevalence, intensity and survival) than the allopatric tested. According to our results, we should note that the assessment of epidemiological risks cannot be limited to a single host–parasite system, but should focus on understanding the diversity of hosts in the heterogeneous environment through space and time. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-10-30 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
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http://hdl.handle.net/11336/131516 Vázquez, Antonio A.; Sabourin, Emeline; Alda, Maria del Pilar; Leroy, Clémentine; Leray, Carole; et al.; Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts; Wiley Blackwell Publishing, Inc; Transboundary And Emerging Diseases; 2020; 30-10-2020; 1-35 1865-1674 1865-1682 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/131516 |
| identifier_str_mv |
Vázquez, Antonio A.; Sabourin, Emeline; Alda, Maria del Pilar; Leroy, Clémentine; Leray, Carole; et al.; Genetic diversity and relationships of the liver fluke Fasciola hepatica (Trematoda) with native and introduced definitive and intermediate hosts; Wiley Blackwell Publishing, Inc; Transboundary And Emerging Diseases; 2020; 30-10-2020; 1-35 1865-1674 1865-1682 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/altIdentifier/doi/10.1111/tbed.13882 info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/tbed.13882 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
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application/pdf application/pdf |
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Wiley Blackwell Publishing, Inc |
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Wiley Blackwell Publishing, Inc |
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