Piezo proteins, mechano reception and behaviour in Hydra

Autores
Alzugaray, Maria Eugenia; Gavazzi, Maria Victoria; Griffo, Lucia; Ronderos, Jorge Rafael
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cells are constantly exposed to mechanical forces. These stimuli must be detected and transduced to maintain homeostasis. Due that reason, mechano-sensory systems (MS) are present in all the organisms to generate appropriate responses. Piezo proteins are a recently discovered family of mechano-gated ion channels that responds to mechanical changes of the plasma membrane, allowing the influx of cations, mainly Ca2+. Piezo MS channels are widely represented in Metazoa, acting in several physiological systems. Hydra sp. is a freshwater member of the phylum Cnidaria which is assumed as the sister group of Bilateria. Despite the existence of Piezo channels in Hydra is known, their physiological roles remain unknown. In this work we delve into the physiological relevance of MS Piezo in responses associated to mechanical stimuli in Hydra sp. We analysed the effects of Jedi1 (a specific agonist of Piezo1) on the contractile behaviours, and cnidocyst discharge, and compared them with responses caused by natural stimuli. The results show that the activation of Piezo channels increases the contractile behaviour, stimulating the occurrence of contraction burst, a sudden kind of retraction observed in response to touch and osmotic stress. Cnidocyst discharge was also induced by Jedi1, resembling the response caused by the contact of the prey. The effects of both Jedi1 and natural stimuli were avoided in the presence of the inhibitor of MS channels GdCl3. The bioinformatic analysis shows that the protein predicted by Hydra genome has the characteristic motifs of Piezo proteins. These results are consistent with the existence of Piezo channels in Hydra, unveiling their physiological roles. Because of the relevance of Piezo channels in several pathological conditions and the high level of conservation in metazoans, Hydra could provide a significative experimental model to assay biological and pharmacological issues.
Fil: Alzugaray, Maria Eugenia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gavazzi, Maria Victoria. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Griffo, Lucia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina
Fil: Ronderos, Jorge Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina
Materia
PIEZO CHANNELS
HYDRA
MECHANORECEPTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271874
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Piezo proteins, mechano reception and behaviour in HydraAlzugaray, Maria EugeniaGavazzi, Maria VictoriaGriffo, LuciaRonderos, Jorge RafaelPIEZO CHANNELSHYDRAMECHANORECEPTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cells are constantly exposed to mechanical forces. These stimuli must be detected and transduced to maintain homeostasis. Due that reason, mechano-sensory systems (MS) are present in all the organisms to generate appropriate responses. Piezo proteins are a recently discovered family of mechano-gated ion channels that responds to mechanical changes of the plasma membrane, allowing the influx of cations, mainly Ca2+. Piezo MS channels are widely represented in Metazoa, acting in several physiological systems. Hydra sp. is a freshwater member of the phylum Cnidaria which is assumed as the sister group of Bilateria. Despite the existence of Piezo channels in Hydra is known, their physiological roles remain unknown. In this work we delve into the physiological relevance of MS Piezo in responses associated to mechanical stimuli in Hydra sp. We analysed the effects of Jedi1 (a specific agonist of Piezo1) on the contractile behaviours, and cnidocyst discharge, and compared them with responses caused by natural stimuli. The results show that the activation of Piezo channels increases the contractile behaviour, stimulating the occurrence of contraction burst, a sudden kind of retraction observed in response to touch and osmotic stress. Cnidocyst discharge was also induced by Jedi1, resembling the response caused by the contact of the prey. The effects of both Jedi1 and natural stimuli were avoided in the presence of the inhibitor of MS channels GdCl3. The bioinformatic analysis shows that the protein predicted by Hydra genome has the characteristic motifs of Piezo proteins. These results are consistent with the existence of Piezo channels in Hydra, unveiling their physiological roles. Because of the relevance of Piezo channels in several pathological conditions and the high level of conservation in metazoans, Hydra could provide a significative experimental model to assay biological and pharmacological issues.Fil: Alzugaray, Maria Eugenia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Gavazzi, Maria Victoria. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Griffo, Lucia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; ArgentinaFil: Ronderos, Jorge Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; ArgentinaNature2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271874Alzugaray, Maria Eugenia; Gavazzi, Maria Victoria; Griffo, Lucia; Ronderos, Jorge Rafael; Piezo proteins, mechano reception and behaviour in Hydra; Nature; Scientific Reports; 15; 1; 2-2025; 1-202045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-025-91048-2info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-025-91048-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:50Zoai:ri.conicet.gov.ar:11336/271874instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:50.437CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Piezo proteins, mechano reception and behaviour in Hydra
title Piezo proteins, mechano reception and behaviour in Hydra
spellingShingle Piezo proteins, mechano reception and behaviour in Hydra
Alzugaray, Maria Eugenia
PIEZO CHANNELS
HYDRA
MECHANORECEPTION
title_short Piezo proteins, mechano reception and behaviour in Hydra
title_full Piezo proteins, mechano reception and behaviour in Hydra
title_fullStr Piezo proteins, mechano reception and behaviour in Hydra
title_full_unstemmed Piezo proteins, mechano reception and behaviour in Hydra
title_sort Piezo proteins, mechano reception and behaviour in Hydra
dc.creator.none.fl_str_mv Alzugaray, Maria Eugenia
Gavazzi, Maria Victoria
Griffo, Lucia
Ronderos, Jorge Rafael
author Alzugaray, Maria Eugenia
author_facet Alzugaray, Maria Eugenia
Gavazzi, Maria Victoria
Griffo, Lucia
Ronderos, Jorge Rafael
author_role author
author2 Gavazzi, Maria Victoria
Griffo, Lucia
Ronderos, Jorge Rafael
author2_role author
author
author
dc.subject.none.fl_str_mv PIEZO CHANNELS
HYDRA
MECHANORECEPTION
topic PIEZO CHANNELS
HYDRA
MECHANORECEPTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cells are constantly exposed to mechanical forces. These stimuli must be detected and transduced to maintain homeostasis. Due that reason, mechano-sensory systems (MS) are present in all the organisms to generate appropriate responses. Piezo proteins are a recently discovered family of mechano-gated ion channels that responds to mechanical changes of the plasma membrane, allowing the influx of cations, mainly Ca2+. Piezo MS channels are widely represented in Metazoa, acting in several physiological systems. Hydra sp. is a freshwater member of the phylum Cnidaria which is assumed as the sister group of Bilateria. Despite the existence of Piezo channels in Hydra is known, their physiological roles remain unknown. In this work we delve into the physiological relevance of MS Piezo in responses associated to mechanical stimuli in Hydra sp. We analysed the effects of Jedi1 (a specific agonist of Piezo1) on the contractile behaviours, and cnidocyst discharge, and compared them with responses caused by natural stimuli. The results show that the activation of Piezo channels increases the contractile behaviour, stimulating the occurrence of contraction burst, a sudden kind of retraction observed in response to touch and osmotic stress. Cnidocyst discharge was also induced by Jedi1, resembling the response caused by the contact of the prey. The effects of both Jedi1 and natural stimuli were avoided in the presence of the inhibitor of MS channels GdCl3. The bioinformatic analysis shows that the protein predicted by Hydra genome has the characteristic motifs of Piezo proteins. These results are consistent with the existence of Piezo channels in Hydra, unveiling their physiological roles. Because of the relevance of Piezo channels in several pathological conditions and the high level of conservation in metazoans, Hydra could provide a significative experimental model to assay biological and pharmacological issues.
Fil: Alzugaray, Maria Eugenia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gavazzi, Maria Victoria. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Griffo, Lucia. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina
Fil: Ronderos, Jorge Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Histología y Embriología Animal; Argentina
description Cells are constantly exposed to mechanical forces. These stimuli must be detected and transduced to maintain homeostasis. Due that reason, mechano-sensory systems (MS) are present in all the organisms to generate appropriate responses. Piezo proteins are a recently discovered family of mechano-gated ion channels that responds to mechanical changes of the plasma membrane, allowing the influx of cations, mainly Ca2+. Piezo MS channels are widely represented in Metazoa, acting in several physiological systems. Hydra sp. is a freshwater member of the phylum Cnidaria which is assumed as the sister group of Bilateria. Despite the existence of Piezo channels in Hydra is known, their physiological roles remain unknown. In this work we delve into the physiological relevance of MS Piezo in responses associated to mechanical stimuli in Hydra sp. We analysed the effects of Jedi1 (a specific agonist of Piezo1) on the contractile behaviours, and cnidocyst discharge, and compared them with responses caused by natural stimuli. The results show that the activation of Piezo channels increases the contractile behaviour, stimulating the occurrence of contraction burst, a sudden kind of retraction observed in response to touch and osmotic stress. Cnidocyst discharge was also induced by Jedi1, resembling the response caused by the contact of the prey. The effects of both Jedi1 and natural stimuli were avoided in the presence of the inhibitor of MS channels GdCl3. The bioinformatic analysis shows that the protein predicted by Hydra genome has the characteristic motifs of Piezo proteins. These results are consistent with the existence of Piezo channels in Hydra, unveiling their physiological roles. Because of the relevance of Piezo channels in several pathological conditions and the high level of conservation in metazoans, Hydra could provide a significative experimental model to assay biological and pharmacological issues.
publishDate 2025
dc.date.none.fl_str_mv 2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271874
Alzugaray, Maria Eugenia; Gavazzi, Maria Victoria; Griffo, Lucia; Ronderos, Jorge Rafael; Piezo proteins, mechano reception and behaviour in Hydra; Nature; Scientific Reports; 15; 1; 2-2025; 1-20
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271874
identifier_str_mv Alzugaray, Maria Eugenia; Gavazzi, Maria Victoria; Griffo, Lucia; Ronderos, Jorge Rafael; Piezo proteins, mechano reception and behaviour in Hydra; Nature; Scientific Reports; 15; 1; 2-2025; 1-20
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-025-91048-2
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-025-91048-2
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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