Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin

Autores
Pourcel, Natalia Gisela; Sparo, Mónica Delfina; Corso, Alejandra; Delpech, Gastón; Gagetti, Paula Silvana; de Luca, Maria Marta; Bernstein, Judith Celina; Schell, Celia María Beatriz; Lisarrague, Sabina; Basualdo Farjat, Juan Angel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enterococci often acquire antimicrobial resistance through horizontal gene transfer. Relatedness between enterococci with high level resistance to gentamicin and vancomycin isolated from humans, food and hospital environment in Tandil County (Argentina) was investigated. PCR amplification for species determination was carried out. Resistance to seven antimicrobials was studied; virulence genes (esp, cylA), vancomycin and gentamicin resistance genes were investigated. In the isolates with high level antimicrobial resistance (gentamicin, vancomycin), pulse-field gel electrophoresis was performed. Vancomycin-resistant E. faecium (n: 13) were recovered from human, food and hospital environment samples. All the isolates expressed high-level vancomycin and teicoplanin (vanA genotype), as well high-level gentamicin and streptomycin resistance. Vancomycin-resistant E. faecium were distributed among seven clonal types; esp gene was detected in clinical strains. There was no clonal relationship with food vanA E. faecium, but these strains could pose a risk in intra/inter genus transfer of vanA determinant to human-adapted strains. High-level gentamicin resistant E. faecalis (n: 7) were recovered from human and food samples. Glycopeptide resistance was not observed; cylA gene was detected in most of the clinical high-level gentamicin resistant E. faecalis isolates. PFGE patterns showed four clonal types in high-level gentamicin resistant E. faecalis strains; there was demonstrated clonal relatedness between isolates from different origin. In Argentina, this is the first study showing a clonal relationship between high-level gentamicin resistant E. faecalis isolated from food and humans. These results encourage the study of dissemination of clonal complexes with mobile resistance genes.
Fil: Pourcel, Natalia Gisela. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Sparo, Mónica Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
Fil: Corso, Alejandra. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Delpech, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Gagetti, Paula Silvana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: de Luca, Maria Marta. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
Fil: Bernstein, Judith Celina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
Fil: Schell, Celia María Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
Fil: Lisarrague, Sabina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
Fil: Basualdo Farjat, Juan Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
Materia
ENTEROCOCCI
RELATEDNESS
FOOD
HUMANS
ENVIRONMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66320
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and VancomycinPourcel, Natalia GiselaSparo, Mónica DelfinaCorso, AlejandraDelpech, GastónGagetti, Paula Silvanade Luca, Maria MartaBernstein, Judith CelinaSchell, Celia María BeatrizLisarrague, SabinaBasualdo Farjat, Juan AngelENTEROCOCCIRELATEDNESSFOODHUMANSENVIRONMENThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Enterococci often acquire antimicrobial resistance through horizontal gene transfer. Relatedness between enterococci with high level resistance to gentamicin and vancomycin isolated from humans, food and hospital environment in Tandil County (Argentina) was investigated. PCR amplification for species determination was carried out. Resistance to seven antimicrobials was studied; virulence genes (esp, cylA), vancomycin and gentamicin resistance genes were investigated. In the isolates with high level antimicrobial resistance (gentamicin, vancomycin), pulse-field gel electrophoresis was performed. Vancomycin-resistant E. faecium (n: 13) were recovered from human, food and hospital environment samples. All the isolates expressed high-level vancomycin and teicoplanin (vanA genotype), as well high-level gentamicin and streptomycin resistance. Vancomycin-resistant E. faecium were distributed among seven clonal types; esp gene was detected in clinical strains. There was no clonal relationship with food vanA E. faecium, but these strains could pose a risk in intra/inter genus transfer of vanA determinant to human-adapted strains. High-level gentamicin resistant E. faecalis (n: 7) were recovered from human and food samples. Glycopeptide resistance was not observed; cylA gene was detected in most of the clinical high-level gentamicin resistant E. faecalis isolates. PFGE patterns showed four clonal types in high-level gentamicin resistant E. faecalis strains; there was demonstrated clonal relatedness between isolates from different origin. In Argentina, this is the first study showing a clonal relationship between high-level gentamicin resistant E. faecalis isolated from food and humans. These results encourage the study of dissemination of clonal complexes with mobile resistance genes.Fil: Pourcel, Natalia Gisela. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Sparo, Mónica Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; ArgentinaFil: Corso, Alejandra. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Delpech, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Gagetti, Paula Silvana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: de Luca, Maria Marta. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; ArgentinaFil: Bernstein, Judith Celina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; ArgentinaFil: Schell, Celia María Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; ArgentinaFil: Lisarrague, Sabina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; ArgentinaFil: Basualdo Farjat, Juan Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; ArgentinaOMICS Publishing Group2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66320Pourcel, Natalia Gisela; Sparo, Mónica Delfina; Corso, Alejandra; Delpech, Gastón; Gagetti, Paula Silvana; et al.; Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin; OMICS Publishing Group; Clinical Microbiology: Open Access; 06; 01; 1-2017; 1-82327-5073CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/molecular-genetic-profiling-of-clinical-and-foodborne-strains-of-enterococci-with-high-level-resistance-to-gentamicin-and-vancomyc-2327-5073-1000272.php?aid=84726info:eu-repo/semantics/altIdentifier/doi/10.4172/2327-5073.1000272info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:21Zoai:ri.conicet.gov.ar:11336/66320instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:22.082CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
title Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
spellingShingle Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
Pourcel, Natalia Gisela
ENTEROCOCCI
RELATEDNESS
FOOD
HUMANS
ENVIRONMENT
title_short Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
title_full Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
title_fullStr Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
title_full_unstemmed Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
title_sort Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
dc.creator.none.fl_str_mv Pourcel, Natalia Gisela
Sparo, Mónica Delfina
Corso, Alejandra
Delpech, Gastón
Gagetti, Paula Silvana
de Luca, Maria Marta
Bernstein, Judith Celina
Schell, Celia María Beatriz
Lisarrague, Sabina
Basualdo Farjat, Juan Angel
author Pourcel, Natalia Gisela
author_facet Pourcel, Natalia Gisela
Sparo, Mónica Delfina
Corso, Alejandra
Delpech, Gastón
Gagetti, Paula Silvana
de Luca, Maria Marta
Bernstein, Judith Celina
Schell, Celia María Beatriz
Lisarrague, Sabina
Basualdo Farjat, Juan Angel
author_role author
author2 Sparo, Mónica Delfina
Corso, Alejandra
Delpech, Gastón
Gagetti, Paula Silvana
de Luca, Maria Marta
Bernstein, Judith Celina
Schell, Celia María Beatriz
Lisarrague, Sabina
Basualdo Farjat, Juan Angel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ENTEROCOCCI
RELATEDNESS
FOOD
HUMANS
ENVIRONMENT
topic ENTEROCOCCI
RELATEDNESS
FOOD
HUMANS
ENVIRONMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Enterococci often acquire antimicrobial resistance through horizontal gene transfer. Relatedness between enterococci with high level resistance to gentamicin and vancomycin isolated from humans, food and hospital environment in Tandil County (Argentina) was investigated. PCR amplification for species determination was carried out. Resistance to seven antimicrobials was studied; virulence genes (esp, cylA), vancomycin and gentamicin resistance genes were investigated. In the isolates with high level antimicrobial resistance (gentamicin, vancomycin), pulse-field gel electrophoresis was performed. Vancomycin-resistant E. faecium (n: 13) were recovered from human, food and hospital environment samples. All the isolates expressed high-level vancomycin and teicoplanin (vanA genotype), as well high-level gentamicin and streptomycin resistance. Vancomycin-resistant E. faecium were distributed among seven clonal types; esp gene was detected in clinical strains. There was no clonal relationship with food vanA E. faecium, but these strains could pose a risk in intra/inter genus transfer of vanA determinant to human-adapted strains. High-level gentamicin resistant E. faecalis (n: 7) were recovered from human and food samples. Glycopeptide resistance was not observed; cylA gene was detected in most of the clinical high-level gentamicin resistant E. faecalis isolates. PFGE patterns showed four clonal types in high-level gentamicin resistant E. faecalis strains; there was demonstrated clonal relatedness between isolates from different origin. In Argentina, this is the first study showing a clonal relationship between high-level gentamicin resistant E. faecalis isolated from food and humans. These results encourage the study of dissemination of clonal complexes with mobile resistance genes.
Fil: Pourcel, Natalia Gisela. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Sparo, Mónica Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
Fil: Corso, Alejandra. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Delpech, Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Gagetti, Paula Silvana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: de Luca, Maria Marta. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
Fil: Bernstein, Judith Celina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
Fil: Schell, Celia María Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
Fil: Lisarrague, Sabina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
Fil: Basualdo Farjat, Juan Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Departamento de Articulación de Ciencias Básicas y Clínicas. Cátedra de Microbiología y Parasitología; Argentina
description Enterococci often acquire antimicrobial resistance through horizontal gene transfer. Relatedness between enterococci with high level resistance to gentamicin and vancomycin isolated from humans, food and hospital environment in Tandil County (Argentina) was investigated. PCR amplification for species determination was carried out. Resistance to seven antimicrobials was studied; virulence genes (esp, cylA), vancomycin and gentamicin resistance genes were investigated. In the isolates with high level antimicrobial resistance (gentamicin, vancomycin), pulse-field gel electrophoresis was performed. Vancomycin-resistant E. faecium (n: 13) were recovered from human, food and hospital environment samples. All the isolates expressed high-level vancomycin and teicoplanin (vanA genotype), as well high-level gentamicin and streptomycin resistance. Vancomycin-resistant E. faecium were distributed among seven clonal types; esp gene was detected in clinical strains. There was no clonal relationship with food vanA E. faecium, but these strains could pose a risk in intra/inter genus transfer of vanA determinant to human-adapted strains. High-level gentamicin resistant E. faecalis (n: 7) were recovered from human and food samples. Glycopeptide resistance was not observed; cylA gene was detected in most of the clinical high-level gentamicin resistant E. faecalis isolates. PFGE patterns showed four clonal types in high-level gentamicin resistant E. faecalis strains; there was demonstrated clonal relatedness between isolates from different origin. In Argentina, this is the first study showing a clonal relationship between high-level gentamicin resistant E. faecalis isolated from food and humans. These results encourage the study of dissemination of clonal complexes with mobile resistance genes.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66320
Pourcel, Natalia Gisela; Sparo, Mónica Delfina; Corso, Alejandra; Delpech, Gastón; Gagetti, Paula Silvana; et al.; Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin; OMICS Publishing Group; Clinical Microbiology: Open Access; 06; 01; 1-2017; 1-8
2327-5073
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66320
identifier_str_mv Pourcel, Natalia Gisela; Sparo, Mónica Delfina; Corso, Alejandra; Delpech, Gastón; Gagetti, Paula Silvana; et al.; Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin; OMICS Publishing Group; Clinical Microbiology: Open Access; 06; 01; 1-2017; 1-8
2327-5073
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/molecular-genetic-profiling-of-clinical-and-foodborne-strains-of-enterococci-with-high-level-resistance-to-gentamicin-and-vancomyc-2327-5073-1000272.php?aid=84726
info:eu-repo/semantics/altIdentifier/doi/10.4172/2327-5073.1000272
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv OMICS Publishing Group
publisher.none.fl_str_mv OMICS Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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