Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins

Autores
Bertrand, Lidwina; Monferran, Magdalena Victoria; Mouneyrac, Catherine; Bonansea, Rocio Ines; Asis, Ramón; Amé, María Valeria
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate the toxic effects of chlorpyrifos (CPF) at environmental concentrations over the shrimp Palaemonetes argentinus, a South American native species. Organisms were exposed in laboratory conditions at relevant environmental concentrations of CPF (from 3.5 to 94.5 ng CPF L-1) for 96 h. A wide battery of biochemical responses including bioaccumulation, damage and defense biomarkers were measured in cephalothorax and abdomen of shrimp. The concentration of CPF was below the detection limit of the method in both body sectors (8 ng CPF g-1 ww), probably indicating the fast biotransformation of the parental compound. Our results showed that CPF exposure inhibits acetylcholinesterase activity from 3.5 ng CPF L-1, a concentration below the suggested Argentinean guidelines for the protection of aquatic biota. Moreover, oxidative stress was evidenced by enhanced H2O2 content and increased levels of TBARs and carbonyl groups in proteins. The induction of antioxidant enzymes like catalase, glutathione S-transferase and glutathione peroxidase seems not be sufficient to prevent oxidative damages. In addition, the mobilization of α-tocopherol from abdomen to cephalothorax was observed and reported for the first time in non-reproductive condition. Likewise, a strong diminution of metallothioneins occurred in cephalothorax from the lowest CPF concentration while induction occurred from the same treatment in abdomen as an oxidative stress response. Finally, significant correlation between Integrated Biomarkers Response values and exposure concentrations suggest the usefulness of P. argentinus as bioindicator of CPF exposure at low dose as environmental concentrations.
Fil: Bertrand, Lidwina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Monferran, Magdalena Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina
Fil: Mouneyrac, Catherine. Université Catholique de l’Ouest; Francia
Fil: Bonansea, Rocio Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
Organophosphate Insecticide
Metallothioneins
Alpha Tocopherol
Native Species
Palaemonetes Argentinus
Oxidative Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48530
Acceder
id CONICETDig_adfb2dd7d6a52faba8b75f5b2deb2c6b
oai_identifier_str oai:ri.conicet.gov.ar:11336/48530
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneinsBertrand, LidwinaMonferran, Magdalena VictoriaMouneyrac, CatherineBonansea, Rocio InesAsis, RamónAmé, María ValeriaOrganophosphate InsecticideMetallothioneinsAlpha TocopherolNative SpeciesPalaemonetes ArgentinusOxidative Stresshttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1The aim of this study was to evaluate the toxic effects of chlorpyrifos (CPF) at environmental concentrations over the shrimp Palaemonetes argentinus, a South American native species. Organisms were exposed in laboratory conditions at relevant environmental concentrations of CPF (from 3.5 to 94.5 ng CPF L-1) for 96 h. A wide battery of biochemical responses including bioaccumulation, damage and defense biomarkers were measured in cephalothorax and abdomen of shrimp. The concentration of CPF was below the detection limit of the method in both body sectors (8 ng CPF g-1 ww), probably indicating the fast biotransformation of the parental compound. Our results showed that CPF exposure inhibits acetylcholinesterase activity from 3.5 ng CPF L-1, a concentration below the suggested Argentinean guidelines for the protection of aquatic biota. Moreover, oxidative stress was evidenced by enhanced H2O2 content and increased levels of TBARs and carbonyl groups in proteins. The induction of antioxidant enzymes like catalase, glutathione S-transferase and glutathione peroxidase seems not be sufficient to prevent oxidative damages. In addition, the mobilization of α-tocopherol from abdomen to cephalothorax was observed and reported for the first time in non-reproductive condition. Likewise, a strong diminution of metallothioneins occurred in cephalothorax from the lowest CPF concentration while induction occurred from the same treatment in abdomen as an oxidative stress response. Finally, significant correlation between Integrated Biomarkers Response values and exposure concentrations suggest the usefulness of P. argentinus as bioindicator of CPF exposure at low dose as environmental concentrations.Fil: Bertrand, Lidwina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Monferran, Magdalena Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Mouneyrac, Catherine. Université Catholique de l’Ouest; FranciaFil: Bonansea, Rocio Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaElsevier Science2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48530Bertrand, Lidwina; Monferran, Magdalena Victoria; Mouneyrac, Catherine; Bonansea, Rocio Ines; Asis, Ramón; et al.; Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins; Elsevier Science; Aquatic Toxicology; 179; 8-2016; 72-810166-445XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166445X16302417info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2016.08.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:25Zoai:ri.conicet.gov.ar:11336/48530instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:26.032CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins
title Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins
spellingShingle Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins
Bertrand, Lidwina
Organophosphate Insecticide
Metallothioneins
Alpha Tocopherol
Native Species
Palaemonetes Argentinus
Oxidative Stress
title_short Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins
title_full Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins
title_fullStr Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins
title_full_unstemmed Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins
title_sort Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins
dc.creator.none.fl_str_mv Bertrand, Lidwina
Monferran, Magdalena Victoria
Mouneyrac, Catherine
Bonansea, Rocio Ines
Asis, Ramón
Amé, María Valeria
author Bertrand, Lidwina
author_facet Bertrand, Lidwina
Monferran, Magdalena Victoria
Mouneyrac, Catherine
Bonansea, Rocio Ines
Asis, Ramón
Amé, María Valeria
author_role author
author2 Monferran, Magdalena Victoria
Mouneyrac, Catherine
Bonansea, Rocio Ines
Asis, Ramón
Amé, María Valeria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Organophosphate Insecticide
Metallothioneins
Alpha Tocopherol
Native Species
Palaemonetes Argentinus
Oxidative Stress
topic Organophosphate Insecticide
Metallothioneins
Alpha Tocopherol
Native Species
Palaemonetes Argentinus
Oxidative Stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to evaluate the toxic effects of chlorpyrifos (CPF) at environmental concentrations over the shrimp Palaemonetes argentinus, a South American native species. Organisms were exposed in laboratory conditions at relevant environmental concentrations of CPF (from 3.5 to 94.5 ng CPF L-1) for 96 h. A wide battery of biochemical responses including bioaccumulation, damage and defense biomarkers were measured in cephalothorax and abdomen of shrimp. The concentration of CPF was below the detection limit of the method in both body sectors (8 ng CPF g-1 ww), probably indicating the fast biotransformation of the parental compound. Our results showed that CPF exposure inhibits acetylcholinesterase activity from 3.5 ng CPF L-1, a concentration below the suggested Argentinean guidelines for the protection of aquatic biota. Moreover, oxidative stress was evidenced by enhanced H2O2 content and increased levels of TBARs and carbonyl groups in proteins. The induction of antioxidant enzymes like catalase, glutathione S-transferase and glutathione peroxidase seems not be sufficient to prevent oxidative damages. In addition, the mobilization of α-tocopherol from abdomen to cephalothorax was observed and reported for the first time in non-reproductive condition. Likewise, a strong diminution of metallothioneins occurred in cephalothorax from the lowest CPF concentration while induction occurred from the same treatment in abdomen as an oxidative stress response. Finally, significant correlation between Integrated Biomarkers Response values and exposure concentrations suggest the usefulness of P. argentinus as bioindicator of CPF exposure at low dose as environmental concentrations.
Fil: Bertrand, Lidwina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Monferran, Magdalena Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina
Fil: Mouneyrac, Catherine. Université Catholique de l’Ouest; Francia
Fil: Bonansea, Rocio Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description The aim of this study was to evaluate the toxic effects of chlorpyrifos (CPF) at environmental concentrations over the shrimp Palaemonetes argentinus, a South American native species. Organisms were exposed in laboratory conditions at relevant environmental concentrations of CPF (from 3.5 to 94.5 ng CPF L-1) for 96 h. A wide battery of biochemical responses including bioaccumulation, damage and defense biomarkers were measured in cephalothorax and abdomen of shrimp. The concentration of CPF was below the detection limit of the method in both body sectors (8 ng CPF g-1 ww), probably indicating the fast biotransformation of the parental compound. Our results showed that CPF exposure inhibits acetylcholinesterase activity from 3.5 ng CPF L-1, a concentration below the suggested Argentinean guidelines for the protection of aquatic biota. Moreover, oxidative stress was evidenced by enhanced H2O2 content and increased levels of TBARs and carbonyl groups in proteins. The induction of antioxidant enzymes like catalase, glutathione S-transferase and glutathione peroxidase seems not be sufficient to prevent oxidative damages. In addition, the mobilization of α-tocopherol from abdomen to cephalothorax was observed and reported for the first time in non-reproductive condition. Likewise, a strong diminution of metallothioneins occurred in cephalothorax from the lowest CPF concentration while induction occurred from the same treatment in abdomen as an oxidative stress response. Finally, significant correlation between Integrated Biomarkers Response values and exposure concentrations suggest the usefulness of P. argentinus as bioindicator of CPF exposure at low dose as environmental concentrations.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48530
Bertrand, Lidwina; Monferran, Magdalena Victoria; Mouneyrac, Catherine; Bonansea, Rocio Ines; Asis, Ramón; et al.; Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins; Elsevier Science; Aquatic Toxicology; 179; 8-2016; 72-81
0166-445X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48530
identifier_str_mv Bertrand, Lidwina; Monferran, Magdalena Victoria; Mouneyrac, Catherine; Bonansea, Rocio Ines; Asis, Ramón; et al.; Sensitive biomarker response of shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Role of alpha-tocopherol and metallothioneins; Elsevier Science; Aquatic Toxicology; 179; 8-2016; 72-81
0166-445X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166445X16302417
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2016.08.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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