Purification of the lipase B of Candida antarctica from a commercial enzymatic extract

Autores
Llerena Suster, Carlos Rafael; Briand, Laura Estefania; Morcelle del Valle, Susana Raquel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This paper presents a rational strategy to purify the lipase B of Candida antarctica of the commercial extract Lipozyme® through size exclusion coupled with anionic exchange chromatography using a non conventional, easy to remove buffer system such as ammonia-ammonium. In this context, each step of the purification was followed through the determination of the protein content, esterase activity measurements, SDS-PAGE, agarose electrophoresis, UVspectroscopy and isoelectric focusing. The purification of the commercial extract afforded a sample that retains 47% of the proteins (being CALB the major enzymatic component of the purified sample) with a hydrolytic activity higher than the starting crude extract.
Fil: Llerena Suster, Carlos Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Ciencias Aplicadas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion de Proteinas Vegetales; Argentina
Fil: Briand, Laura Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Ciencias Aplicadas; Argentina
Fil: Morcelle del Valle, Susana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina
Materia
Protein Purification
Calb
Uv Absorbance
Bradford
Size Exclusion Chromatography
Ion Exchange Chromatography
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7530
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7530
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Purification of the lipase B of Candida antarctica from a commercial enzymatic extractLlerena Suster, Carlos RafaelBriand, Laura EstefaniaMorcelle del Valle, Susana RaquelProtein PurificationCalbUv AbsorbanceBradfordSize Exclusion ChromatographyIon Exchange Chromatographyhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2This paper presents a rational strategy to purify the lipase B of Candida antarctica of the commercial extract Lipozyme® through size exclusion coupled with anionic exchange chromatography using a non conventional, easy to remove buffer system such as ammonia-ammonium. In this context, each step of the purification was followed through the determination of the protein content, esterase activity measurements, SDS-PAGE, agarose electrophoresis, UVspectroscopy and isoelectric focusing. The purification of the commercial extract afforded a sample that retains 47% of the proteins (being CALB the major enzymatic component of the purified sample) with a hydrolytic activity higher than the starting crude extract.Fil: Llerena Suster, Carlos Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Ciencias Aplicadas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion de Proteinas Vegetales; ArgentinaFil: Briand, Laura Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Ciencias Aplicadas; ArgentinaFil: Morcelle del Valle, Susana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas; ArgentinaBentham Science Publishers2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7530Llerena Suster, Carlos Rafael; Briand, Laura Estefania; Morcelle del Valle, Susana Raquel; Purification of the lipase B of Candida antarctica from a commercial enzymatic extract; Bentham Science Publishers; Current Catalysis; 3; 2; 1-2014; 155-1602211-5447enginfo:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/ben/ccat/2014/00000003/00000002/art00007info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/119162info:eu-repo/semantics/altIdentifier/doi/10.2174/2211544702666131224234223info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:02:18Zoai:ri.conicet.gov.ar:11336/7530instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:02:19.165CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Purification of the lipase B of Candida antarctica from a commercial enzymatic extract
title Purification of the lipase B of Candida antarctica from a commercial enzymatic extract
spellingShingle Purification of the lipase B of Candida antarctica from a commercial enzymatic extract
Llerena Suster, Carlos Rafael
Protein Purification
Calb
Uv Absorbance
Bradford
Size Exclusion Chromatography
Ion Exchange Chromatography
title_short Purification of the lipase B of Candida antarctica from a commercial enzymatic extract
title_full Purification of the lipase B of Candida antarctica from a commercial enzymatic extract
title_fullStr Purification of the lipase B of Candida antarctica from a commercial enzymatic extract
title_full_unstemmed Purification of the lipase B of Candida antarctica from a commercial enzymatic extract
title_sort Purification of the lipase B of Candida antarctica from a commercial enzymatic extract
dc.creator.none.fl_str_mv Llerena Suster, Carlos Rafael
Briand, Laura Estefania
Morcelle del Valle, Susana Raquel
author Llerena Suster, Carlos Rafael
author_facet Llerena Suster, Carlos Rafael
Briand, Laura Estefania
Morcelle del Valle, Susana Raquel
author_role author
author2 Briand, Laura Estefania
Morcelle del Valle, Susana Raquel
author2_role author
author
dc.subject.none.fl_str_mv Protein Purification
Calb
Uv Absorbance
Bradford
Size Exclusion Chromatography
Ion Exchange Chromatography
topic Protein Purification
Calb
Uv Absorbance
Bradford
Size Exclusion Chromatography
Ion Exchange Chromatography
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This paper presents a rational strategy to purify the lipase B of Candida antarctica of the commercial extract Lipozyme® through size exclusion coupled with anionic exchange chromatography using a non conventional, easy to remove buffer system such as ammonia-ammonium. In this context, each step of the purification was followed through the determination of the protein content, esterase activity measurements, SDS-PAGE, agarose electrophoresis, UVspectroscopy and isoelectric focusing. The purification of the commercial extract afforded a sample that retains 47% of the proteins (being CALB the major enzymatic component of the purified sample) with a hydrolytic activity higher than the starting crude extract.
Fil: Llerena Suster, Carlos Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Ciencias Aplicadas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biologicas. Laboratorio de Investigacion de Proteinas Vegetales; Argentina
Fil: Briand, Laura Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Ciencias Aplicadas; Argentina
Fil: Morcelle del Valle, Susana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina
description This paper presents a rational strategy to purify the lipase B of Candida antarctica of the commercial extract Lipozyme® through size exclusion coupled with anionic exchange chromatography using a non conventional, easy to remove buffer system such as ammonia-ammonium. In this context, each step of the purification was followed through the determination of the protein content, esterase activity measurements, SDS-PAGE, agarose electrophoresis, UVspectroscopy and isoelectric focusing. The purification of the commercial extract afforded a sample that retains 47% of the proteins (being CALB the major enzymatic component of the purified sample) with a hydrolytic activity higher than the starting crude extract.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7530
Llerena Suster, Carlos Rafael; Briand, Laura Estefania; Morcelle del Valle, Susana Raquel; Purification of the lipase B of Candida antarctica from a commercial enzymatic extract; Bentham Science Publishers; Current Catalysis; 3; 2; 1-2014; 155-160
2211-5447
url http://hdl.handle.net/11336/7530
identifier_str_mv Llerena Suster, Carlos Rafael; Briand, Laura Estefania; Morcelle del Valle, Susana Raquel; Purification of the lipase B of Candida antarctica from a commercial enzymatic extract; Bentham Science Publishers; Current Catalysis; 3; 2; 1-2014; 155-160
2211-5447
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/ben/ccat/2014/00000003/00000002/art00007
info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/119162
info:eu-repo/semantics/altIdentifier/doi/10.2174/2211544702666131224234223
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782358578003968
score 12.982451

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