DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis

Autores
Pomponio, Maria Florencia; Torales, Susana; Gallo, Leonardo; Pastorino, Mario Juan; Marchelli, Paula; Cervera, María Teresa; Marcucci Poltri, Susana Noemí
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Austrocedrus chilensis (D. Don) Pic. Ser. et Bizzarri commonly known as Patagonian cypress is a member of the Cupressaceae family, characterized by a high adaptive potential for growing in marginal areas and good timber quality. The species grows over a wide area and under a wide range of rainfall. This study assessed adaptive genetic variation at SNP level in candidate genes involved in response to drought stress. Results: A total of 18 single nucleotide polymorphisms (SNPs) were found among 1,428 bp. Average nucleotide diversity value (π = 0.00312) was similar to those previously reported in other Cupressaceae. The Fst average among genes and populations was 0.163 and the lowest differentiation was observed in continuous and humid populations. A number of neutrality tests were applied to find evidence of positive selection in our candidate gene set, but only AcAQP2 gene in Pedregoso and San Ramón populations revealed significant departures from neutrality with positive values suggesting balancing selection. Conclusions: In this study we report the levels of nucleotide diversity searched in some drought stress candidate genes in Austrocedrus chilensis and the selective factors that may be acting on this species.
Fil: Pomponio, Maria Florencia. Instituto Nacional de Tecnologia Agropecuaria. Centro Nacional de Inv. Agropecuarias. Centro de Invest.de Recursos Naturales. Instituto de Recursos Biologicos; Argentina
Fil: Torales, Susana. Instituto Nacional de Tecnologia Agropecuaria. Centro Nacional de Inv. Agropecuarias. Centro de Invest.de Recursos Naturales. Instituto de Recursos Biologicos; Argentina
Fil: Gallo, Leonardo. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.patagonia Norte. Estacion Exptal.agrop.s.c.de Bariloche. Grupo de Genetica Forestal; Argentina
Fil: Pastorino, Mario Juan. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.patagonia Norte. Estacion Exptal.agrop.s.c.de Bariloche. Grupo de Genetica Forestal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marchelli, Paula. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.patagonia Norte. Estacion Exptal.agrop.s.c.de Bariloche. Grupo de Genetica Forestal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cervera, María Teresa. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Centro de Investigación Forestal; España
Fil: Marcucci Poltri, Susana Noemí. Instituto Nacional de Tecnologia Agropecuaria. Centro Nacional de Inv. Agropecuarias. Centro de Invest.de Cs.veterinarias y Agronomicas. Instituto de Biotecnologia; Argentina
Materia
ADAPTIVE VARIATION
BALANCING SELECTION
PATAGONIAN CYPRESS
SINGLE NUCLEOTIDE POLYMORPHISM (SNPs)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19366
Acceder
id CONICETDig_ad24ab0bd81b476e6ea62bea29ca2fb7
oai_identifier_str oai:ri.conicet.gov.ar:11336/19366
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling DNA sequence variation of drought-response candidate genes in Austrocedrus chilensisPomponio, Maria FlorenciaTorales, SusanaGallo, LeonardoPastorino, Mario JuanMarchelli, PaulaCervera, María TeresaMarcucci Poltri, Susana NoemíADAPTIVE VARIATIONBALANCING SELECTIONPATAGONIAN CYPRESSSINGLE NUCLEOTIDE POLYMORPHISM (SNPs)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Austrocedrus chilensis (D. Don) Pic. Ser. et Bizzarri commonly known as Patagonian cypress is a member of the Cupressaceae family, characterized by a high adaptive potential for growing in marginal areas and good timber quality. The species grows over a wide area and under a wide range of rainfall. This study assessed adaptive genetic variation at SNP level in candidate genes involved in response to drought stress. Results: A total of 18 single nucleotide polymorphisms (SNPs) were found among 1,428 bp. Average nucleotide diversity value (π = 0.00312) was similar to those previously reported in other Cupressaceae. The Fst average among genes and populations was 0.163 and the lowest differentiation was observed in continuous and humid populations. A number of neutrality tests were applied to find evidence of positive selection in our candidate gene set, but only AcAQP2 gene in Pedregoso and San Ramón populations revealed significant departures from neutrality with positive values suggesting balancing selection. Conclusions: In this study we report the levels of nucleotide diversity searched in some drought stress candidate genes in Austrocedrus chilensis and the selective factors that may be acting on this species.Fil: Pomponio, Maria Florencia. Instituto Nacional de Tecnologia Agropecuaria. Centro Nacional de Inv. Agropecuarias. Centro de Invest.de Recursos Naturales. Instituto de Recursos Biologicos; ArgentinaFil: Torales, Susana. Instituto Nacional de Tecnologia Agropecuaria. Centro Nacional de Inv. Agropecuarias. Centro de Invest.de Recursos Naturales. Instituto de Recursos Biologicos; ArgentinaFil: Gallo, Leonardo. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.patagonia Norte. Estacion Exptal.agrop.s.c.de Bariloche. Grupo de Genetica Forestal; ArgentinaFil: Pastorino, Mario Juan. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.patagonia Norte. Estacion Exptal.agrop.s.c.de Bariloche. Grupo de Genetica Forestal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marchelli, Paula. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.patagonia Norte. Estacion Exptal.agrop.s.c.de Bariloche. Grupo de Genetica Forestal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cervera, María Teresa. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Centro de Investigación Forestal; EspañaFil: Marcucci Poltri, Susana Noemí. Instituto Nacional de Tecnologia Agropecuaria. Centro Nacional de Inv. Agropecuarias. Centro de Invest.de Cs.veterinarias y Agronomicas. Instituto de Biotecnologia; ArgentinaUniv Catolica de Valparaiso2013-03-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19366Pomponio, Maria Florencia; Torales, Susana; Gallo, Leonardo; Pastorino, Mario Juan; Marchelli, Paula; et al.; DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 16; 2; 8-3-2013; 1-120717-3458CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/1045info:eu-repo/semantics/altIdentifier/doi/10.2225/vol16-issue2-fulltext-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:30Zoai:ri.conicet.gov.ar:11336/19366instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:31.081CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis
title DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis
spellingShingle DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis
Pomponio, Maria Florencia
ADAPTIVE VARIATION
BALANCING SELECTION
PATAGONIAN CYPRESS
SINGLE NUCLEOTIDE POLYMORPHISM (SNPs)
title_short DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis
title_full DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis
title_fullStr DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis
title_full_unstemmed DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis
title_sort DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis
dc.creator.none.fl_str_mv Pomponio, Maria Florencia
Torales, Susana
Gallo, Leonardo
Pastorino, Mario Juan
Marchelli, Paula
Cervera, María Teresa
Marcucci Poltri, Susana Noemí
author Pomponio, Maria Florencia
author_facet Pomponio, Maria Florencia
Torales, Susana
Gallo, Leonardo
Pastorino, Mario Juan
Marchelli, Paula
Cervera, María Teresa
Marcucci Poltri, Susana Noemí
author_role author
author2 Torales, Susana
Gallo, Leonardo
Pastorino, Mario Juan
Marchelli, Paula
Cervera, María Teresa
Marcucci Poltri, Susana Noemí
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ADAPTIVE VARIATION
BALANCING SELECTION
PATAGONIAN CYPRESS
SINGLE NUCLEOTIDE POLYMORPHISM (SNPs)
topic ADAPTIVE VARIATION
BALANCING SELECTION
PATAGONIAN CYPRESS
SINGLE NUCLEOTIDE POLYMORPHISM (SNPs)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Austrocedrus chilensis (D. Don) Pic. Ser. et Bizzarri commonly known as Patagonian cypress is a member of the Cupressaceae family, characterized by a high adaptive potential for growing in marginal areas and good timber quality. The species grows over a wide area and under a wide range of rainfall. This study assessed adaptive genetic variation at SNP level in candidate genes involved in response to drought stress. Results: A total of 18 single nucleotide polymorphisms (SNPs) were found among 1,428 bp. Average nucleotide diversity value (π = 0.00312) was similar to those previously reported in other Cupressaceae. The Fst average among genes and populations was 0.163 and the lowest differentiation was observed in continuous and humid populations. A number of neutrality tests were applied to find evidence of positive selection in our candidate gene set, but only AcAQP2 gene in Pedregoso and San Ramón populations revealed significant departures from neutrality with positive values suggesting balancing selection. Conclusions: In this study we report the levels of nucleotide diversity searched in some drought stress candidate genes in Austrocedrus chilensis and the selective factors that may be acting on this species.
Fil: Pomponio, Maria Florencia. Instituto Nacional de Tecnologia Agropecuaria. Centro Nacional de Inv. Agropecuarias. Centro de Invest.de Recursos Naturales. Instituto de Recursos Biologicos; Argentina
Fil: Torales, Susana. Instituto Nacional de Tecnologia Agropecuaria. Centro Nacional de Inv. Agropecuarias. Centro de Invest.de Recursos Naturales. Instituto de Recursos Biologicos; Argentina
Fil: Gallo, Leonardo. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.patagonia Norte. Estacion Exptal.agrop.s.c.de Bariloche. Grupo de Genetica Forestal; Argentina
Fil: Pastorino, Mario Juan. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.patagonia Norte. Estacion Exptal.agrop.s.c.de Bariloche. Grupo de Genetica Forestal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marchelli, Paula. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.patagonia Norte. Estacion Exptal.agrop.s.c.de Bariloche. Grupo de Genetica Forestal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cervera, María Teresa. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Centro de Investigación Forestal; España
Fil: Marcucci Poltri, Susana Noemí. Instituto Nacional de Tecnologia Agropecuaria. Centro Nacional de Inv. Agropecuarias. Centro de Invest.de Cs.veterinarias y Agronomicas. Instituto de Biotecnologia; Argentina
description Background: Austrocedrus chilensis (D. Don) Pic. Ser. et Bizzarri commonly known as Patagonian cypress is a member of the Cupressaceae family, characterized by a high adaptive potential for growing in marginal areas and good timber quality. The species grows over a wide area and under a wide range of rainfall. This study assessed adaptive genetic variation at SNP level in candidate genes involved in response to drought stress. Results: A total of 18 single nucleotide polymorphisms (SNPs) were found among 1,428 bp. Average nucleotide diversity value (π = 0.00312) was similar to those previously reported in other Cupressaceae. The Fst average among genes and populations was 0.163 and the lowest differentiation was observed in continuous and humid populations. A number of neutrality tests were applied to find evidence of positive selection in our candidate gene set, but only AcAQP2 gene in Pedregoso and San Ramón populations revealed significant departures from neutrality with positive values suggesting balancing selection. Conclusions: In this study we report the levels of nucleotide diversity searched in some drought stress candidate genes in Austrocedrus chilensis and the selective factors that may be acting on this species.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19366
Pomponio, Maria Florencia; Torales, Susana; Gallo, Leonardo; Pastorino, Mario Juan; Marchelli, Paula; et al.; DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 16; 2; 8-3-2013; 1-12
0717-3458
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19366
identifier_str_mv Pomponio, Maria Florencia; Torales, Susana; Gallo, Leonardo; Pastorino, Mario Juan; Marchelli, Paula; et al.; DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 16; 2; 8-3-2013; 1-12
0717-3458
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/1045
info:eu-repo/semantics/altIdentifier/doi/10.2225/vol16-issue2-fulltext-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Univ Catolica de Valparaiso
publisher.none.fl_str_mv Univ Catolica de Valparaiso
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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