A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells

Autores
Wagner, Paula Micaela; Sosa Alderete, Lucas Gastón; Gorne, Lucas Damián; Gaveglio, Virginia Lucía; Salvador, Gabriela Alejandra; Pasquaré, Susana Juana; Guido, Mario Eduardo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Rat spermatogenic cell membranes contain sphingolipids with nonhydroxy and 2-hydroxy very long chain (C24-32) PUFA. The biosynthesis of such fatty acids requires the expression of very long chain fatty acid elongases (Elovl4 for > C24) and a fatty acid 2- hydroxylase (Fa2h). In this study, mRNA levels of Elovl4and Fa2h were measured by qPCR in rat testis at different postnatal ages and in cells isolated from the seminiferous epithelium of adults. At early prepuberal ages (P14), Elovl4 was highly expressed while Fa2h mRNA was absent. Fa2h started to be detected at P25-30 and increased thereafter, while Elovl4mRNA levels decreased. The expression of both genes, but mainly Fa2h, was markedly reduced in adult testes that had been depleted of germ cells by mild hyperthermia. In isolated spermatogenic cells, both genes were expressed at lower levels in pachytene spermatocytes than in post meiotic round and late spermatids. Interestingly, Sertoli cells had high Elovl4but lacked Fa2h mRNA. The Elovl4 protein was detected in spermatocytes from P21 to adulthood, when the protein was clearly observed in elongated spermatids. The Elovl4 enzyme was functional in germ cells, as these cells, in culture, were able to elongate [3H]20:4 to PUFA longer than C24. Our results underscore the presence of a well-timed, cell-specific regulation of Elovl4 and Fa2h in germ cells as differentiation proceeds.
Fil: Wagner, Paula Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Sosa Alderete, Lucas Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Gorne, Lucas Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Guido, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Córdoba
Argentina
Universidad Nacional de Córdoba
Materia
GLIOBLASTOMA
CIRCADIAN CLOCK
LIPIDS
PHOSPHOLIPIDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156379
Acceder
id CONICETDig_ad1e98550a6e6bdb88934351d50cbc99
oai_identifier_str oai:ri.conicet.gov.ar:11336/156379
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cellsWagner, Paula MicaelaSosa Alderete, Lucas GastónGorne, Lucas DamiánGaveglio, Virginia LucíaSalvador, Gabriela AlejandraPasquaré, Susana JuanaGuido, Mario EduardoGLIOBLASTOMACIRCADIAN CLOCKLIPIDSPHOSPHOLIPIDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rat spermatogenic cell membranes contain sphingolipids with nonhydroxy and 2-hydroxy very long chain (C24-32) PUFA. The biosynthesis of such fatty acids requires the expression of very long chain fatty acid elongases (Elovl4 for > C24) and a fatty acid 2- hydroxylase (Fa2h). In this study, mRNA levels of Elovl4and Fa2h were measured by qPCR in rat testis at different postnatal ages and in cells isolated from the seminiferous epithelium of adults. At early prepuberal ages (P14), Elovl4 was highly expressed while Fa2h mRNA was absent. Fa2h started to be detected at P25-30 and increased thereafter, while Elovl4mRNA levels decreased. The expression of both genes, but mainly Fa2h, was markedly reduced in adult testes that had been depleted of germ cells by mild hyperthermia. In isolated spermatogenic cells, both genes were expressed at lower levels in pachytene spermatocytes than in post meiotic round and late spermatids. Interestingly, Sertoli cells had high Elovl4but lacked Fa2h mRNA. The Elovl4 protein was detected in spermatocytes from P21 to adulthood, when the protein was clearly observed in elongated spermatids. The Elovl4 enzyme was functional in germ cells, as these cells, in culture, were able to elongate [3H]20:4 to PUFA longer than C24. Our results underscore the presence of a well-timed, cell-specific regulation of Elovl4 and Fa2h in germ cells as differentiation proceeds.Fil: Wagner, Paula Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Sosa Alderete, Lucas Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Gorne, Lucas Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Guido, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina52 th Annual Meeting Argentine Society for Biochemistry and Molecular BiologyCórdobaArgentinaUniversidad Nacional de CórdobaTech Science Press2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156379A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells; 52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Córdoba; Argentina; 2016; 37-371667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/index.php?q=node/562info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2016.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:18Zoai:ri.conicet.gov.ar:11336/156379instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:19.002CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells
title A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells
spellingShingle A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells
Wagner, Paula Micaela
GLIOBLASTOMA
CIRCADIAN CLOCK
LIPIDS
PHOSPHOLIPIDS
title_short A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells
title_full A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells
title_fullStr A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells
title_full_unstemmed A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells
title_sort A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells
dc.creator.none.fl_str_mv Wagner, Paula Micaela
Sosa Alderete, Lucas Gastón
Gorne, Lucas Damián
Gaveglio, Virginia Lucía
Salvador, Gabriela Alejandra
Pasquaré, Susana Juana
Guido, Mario Eduardo
author Wagner, Paula Micaela
author_facet Wagner, Paula Micaela
Sosa Alderete, Lucas Gastón
Gorne, Lucas Damián
Gaveglio, Virginia Lucía
Salvador, Gabriela Alejandra
Pasquaré, Susana Juana
Guido, Mario Eduardo
author_role author
author2 Sosa Alderete, Lucas Gastón
Gorne, Lucas Damián
Gaveglio, Virginia Lucía
Salvador, Gabriela Alejandra
Pasquaré, Susana Juana
Guido, Mario Eduardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv GLIOBLASTOMA
CIRCADIAN CLOCK
LIPIDS
PHOSPHOLIPIDS
topic GLIOBLASTOMA
CIRCADIAN CLOCK
LIPIDS
PHOSPHOLIPIDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rat spermatogenic cell membranes contain sphingolipids with nonhydroxy and 2-hydroxy very long chain (C24-32) PUFA. The biosynthesis of such fatty acids requires the expression of very long chain fatty acid elongases (Elovl4 for > C24) and a fatty acid 2- hydroxylase (Fa2h). In this study, mRNA levels of Elovl4and Fa2h were measured by qPCR in rat testis at different postnatal ages and in cells isolated from the seminiferous epithelium of adults. At early prepuberal ages (P14), Elovl4 was highly expressed while Fa2h mRNA was absent. Fa2h started to be detected at P25-30 and increased thereafter, while Elovl4mRNA levels decreased. The expression of both genes, but mainly Fa2h, was markedly reduced in adult testes that had been depleted of germ cells by mild hyperthermia. In isolated spermatogenic cells, both genes were expressed at lower levels in pachytene spermatocytes than in post meiotic round and late spermatids. Interestingly, Sertoli cells had high Elovl4but lacked Fa2h mRNA. The Elovl4 protein was detected in spermatocytes from P21 to adulthood, when the protein was clearly observed in elongated spermatids. The Elovl4 enzyme was functional in germ cells, as these cells, in culture, were able to elongate [3H]20:4 to PUFA longer than C24. Our results underscore the presence of a well-timed, cell-specific regulation of Elovl4 and Fa2h in germ cells as differentiation proceeds.
Fil: Wagner, Paula Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Sosa Alderete, Lucas Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Gorne, Lucas Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Guido, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Córdoba
Argentina
Universidad Nacional de Córdoba
description Rat spermatogenic cell membranes contain sphingolipids with nonhydroxy and 2-hydroxy very long chain (C24-32) PUFA. The biosynthesis of such fatty acids requires the expression of very long chain fatty acid elongases (Elovl4 for > C24) and a fatty acid 2- hydroxylase (Fa2h). In this study, mRNA levels of Elovl4and Fa2h were measured by qPCR in rat testis at different postnatal ages and in cells isolated from the seminiferous epithelium of adults. At early prepuberal ages (P14), Elovl4 was highly expressed while Fa2h mRNA was absent. Fa2h started to be detected at P25-30 and increased thereafter, while Elovl4mRNA levels decreased. The expression of both genes, but mainly Fa2h, was markedly reduced in adult testes that had been depleted of germ cells by mild hyperthermia. In isolated spermatogenic cells, both genes were expressed at lower levels in pachytene spermatocytes than in post meiotic round and late spermatids. Interestingly, Sertoli cells had high Elovl4but lacked Fa2h mRNA. The Elovl4 protein was detected in spermatocytes from P21 to adulthood, when the protein was clearly observed in elongated spermatids. The Elovl4 enzyme was functional in germ cells, as these cells, in culture, were able to elongate [3H]20:4 to PUFA longer than C24. Our results underscore the presence of a well-timed, cell-specific regulation of Elovl4 and Fa2h in germ cells as differentiation proceeds.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156379
A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells; 52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Córdoba; Argentina; 2016; 37-37
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156379
identifier_str_mv A metabolic circadian clock controls rhythms in immortalized human glioblastoma T98G cells; 52 th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Córdoba; Argentina; 2016; 37-37
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/index.php?q=node/562
info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2016.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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