The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver

Autores
Parodi, Armando José A.; Behrens, Nicolás H.; Leloir, Luis Federico; Carminatti, Hector
Año de publicación
1972
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It has been reported that liver microsomes catalyze the transfer of glucose from uridine diphosphate glucose to dolichol monophosphate so as to produce dolichol monophosphate glucose. Dolichol is a polyprenol containing about 20 isoprene units. The glucosyl residue of dolichol monophosphate glucose is transferred to an endogenous acceptor on further incubation with liver microsomes. The glucosylated endogenous acceptor appears to be an oligosaccharide of about 20 monosaccharide units bound to dolichol through a phosphate or pyrophosphate bridge. In this paper it is reported that liver microsomes catalyze the transfer of the oligosaccharide from the glucosylated endogenous acceptor to an endogenous protein. This transfer reaction requires the presence of bivalent cations, manganese being more effective than magnesium. The presence of deoxycholate is also required. Besides the glycoprotein, several water-soluble products are also formed. Preliminary evidence indicates that they are glucose, iligosaccharides of different size, and possibly oligosaccharides bound to amino acids.
original
Fil: Parodi, Armando José A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina
Fil: Behrens, Nicolás H.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina
Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina
Fil: Carminatti, Hector. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina
5 páginas en pdf
LFL-PI-O-ART. Artículos científicos
Unidad documental simple
AR-HYL-2018
Materia
Leloir, Luis F.
Behrens, Nicolás H.
Parodi, Armando J.
Carminatti, Héctor
Leloir Investigador
Publicaciones
Artículos Científicos
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/135990
Acceder
id CONICETDig_acb88cb6930daed527deb5236c3049ce
oai_identifier_str oai:ri.conicet.gov.ar:11336/135990
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liverParodi, Armando José A.Behrens, Nicolás H.Leloir, Luis FedericoCarminatti, HectorLeloir, Luis F.Behrens, Nicolás H.Parodi, Armando J.Carminatti, HéctorLeloir InvestigadorPublicacionesArtículos CientíficosLos Trabajos de LeloirLos Trabajos del InstitutoInstituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloirhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1It has been reported that liver microsomes catalyze the transfer of glucose from uridine diphosphate glucose to dolichol monophosphate so as to produce dolichol monophosphate glucose. Dolichol is a polyprenol containing about 20 isoprene units. The glucosyl residue of dolichol monophosphate glucose is transferred to an endogenous acceptor on further incubation with liver microsomes. The glucosylated endogenous acceptor appears to be an oligosaccharide of about 20 monosaccharide units bound to dolichol through a phosphate or pyrophosphate bridge. In this paper it is reported that liver microsomes catalyze the transfer of the oligosaccharide from the glucosylated endogenous acceptor to an endogenous protein. This transfer reaction requires the presence of bivalent cations, manganese being more effective than magnesium. The presence of deoxycholate is also required. Besides the glycoprotein, several water-soluble products are also formed. Preliminary evidence indicates that they are glucose, iligosaccharides of different size, and possibly oligosaccharides bound to amino acids.originalFil: Parodi, Armando José A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; ArgentinaFil: Behrens, Nicolás H.. Instituto de Investigaciones Bioquímicas Fundación Campomar; ArgentinaFil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; ArgentinaFil: Carminatti, Hector. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina5 páginas en pdfLFL-PI-O-ART. Artículos científicosUnidad documental simpleAR-HYL-2018National Academy of Sciences1972-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfDerivadaBlancoNegroartículoNoapplication/pdfhttp://hdl.handle.net/11336/135990Parodi, Armando José A.; Behrens, Nicolás H.; Leloir, Luis Federico; Carminatti, Hector; The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 69; 11; 11-1972; 3268-720027-8424enginfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.69.11.3268info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/4508319/Ciudad de Buenos AiresFundación Instituto LeloirConsejo Nacional de Investigaciones Científicas y TécnicasProyecto Houssay y LeloirLeloir, Luis F.LFL-PI-O-ART.178info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-04-08T11:30:11Zoai:ri.conicet.gov.ar:11336/135990instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-04-08 11:30:11.707CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver
title The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver
spellingShingle The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver
Parodi, Armando José A.
Leloir, Luis F.
Behrens, Nicolás H.
Parodi, Armando J.
Carminatti, Héctor
Leloir Investigador
Publicaciones
Artículos Científicos
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
title_short The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver
title_full The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver
title_fullStr The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver
title_full_unstemmed The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver
title_sort The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver
dc.creator.none.fl_str_mv Parodi, Armando José A.
Behrens, Nicolás H.
Leloir, Luis Federico
Carminatti, Hector
author Parodi, Armando José A.
author_facet Parodi, Armando José A.
Behrens, Nicolás H.
Leloir, Luis Federico
Carminatti, Hector
author_role author
author2 Behrens, Nicolás H.
Leloir, Luis Federico
Carminatti, Hector
author2_role author
author
author
dc.subject.none.fl_str_mv Leloir, Luis F.
Behrens, Nicolás H.
Parodi, Armando J.
Carminatti, Héctor
Leloir Investigador
Publicaciones
Artículos Científicos
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
topic Leloir, Luis F.
Behrens, Nicolás H.
Parodi, Armando J.
Carminatti, Héctor
Leloir Investigador
Publicaciones
Artículos Científicos
Los Trabajos de Leloir
Los Trabajos del Instituto
Instituto de Investigaciones Bioquímicas Fundación Campomar ahora Fundación Instituto Leloir
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv It has been reported that liver microsomes catalyze the transfer of glucose from uridine diphosphate glucose to dolichol monophosphate so as to produce dolichol monophosphate glucose. Dolichol is a polyprenol containing about 20 isoprene units. The glucosyl residue of dolichol monophosphate glucose is transferred to an endogenous acceptor on further incubation with liver microsomes. The glucosylated endogenous acceptor appears to be an oligosaccharide of about 20 monosaccharide units bound to dolichol through a phosphate or pyrophosphate bridge. In this paper it is reported that liver microsomes catalyze the transfer of the oligosaccharide from the glucosylated endogenous acceptor to an endogenous protein. This transfer reaction requires the presence of bivalent cations, manganese being more effective than magnesium. The presence of deoxycholate is also required. Besides the glycoprotein, several water-soluble products are also formed. Preliminary evidence indicates that they are glucose, iligosaccharides of different size, and possibly oligosaccharides bound to amino acids.
original
Fil: Parodi, Armando José A.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina
Fil: Behrens, Nicolás H.. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina
Fil: Leloir, Luis Federico. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina
Fil: Carminatti, Hector. Instituto de Investigaciones Bioquímicas Fundación Campomar; Argentina
5 páginas en pdf
LFL-PI-O-ART. Artículos científicos
Unidad documental simple
AR-HYL-2018
description It has been reported that liver microsomes catalyze the transfer of glucose from uridine diphosphate glucose to dolichol monophosphate so as to produce dolichol monophosphate glucose. Dolichol is a polyprenol containing about 20 isoprene units. The glucosyl residue of dolichol monophosphate glucose is transferred to an endogenous acceptor on further incubation with liver microsomes. The glucosylated endogenous acceptor appears to be an oligosaccharide of about 20 monosaccharide units bound to dolichol through a phosphate or pyrophosphate bridge. In this paper it is reported that liver microsomes catalyze the transfer of the oligosaccharide from the glucosylated endogenous acceptor to an endogenous protein. This transfer reaction requires the presence of bivalent cations, manganese being more effective than magnesium. The presence of deoxycholate is also required. Besides the glycoprotein, several water-soluble products are also formed. Preliminary evidence indicates that they are glucose, iligosaccharides of different size, and possibly oligosaccharides bound to amino acids.
publishDate 1972
dc.date.none.fl_str_mv 1972-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/135990
Parodi, Armando José A.; Behrens, Nicolás H.; Leloir, Luis Federico; Carminatti, Hector; The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 69; 11; 11-1972; 3268-72
0027-8424
url http://hdl.handle.net/11336/135990
identifier_str_mv Parodi, Armando José A.; Behrens, Nicolás H.; Leloir, Luis Federico; Carminatti, Hector; The role of polyprenol-bound saccharides as intermediates in glycoprotein synthesis in liver; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 69; 11; 11-1972; 3268-72
0027-8424
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.69.11.3268
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/4508319/
Ciudad de Buenos Aires
Fundación Instituto Leloir
Consejo Nacional de Investigaciones Científicas y Técnicas
Proyecto Houssay y Leloir
Leloir, Luis F.
LFL-PI-O-ART.178
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
Derivada
BlancoNegro
artículo
No
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1861930921332047872
score 13.018236

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