Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore

Autores
Salerno, Claudia; Cuestas, María Luján; Manco, Karina; Chiappetta, Diego Andrés; Lucangioli, Silvia Edith
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Self-assembled drug delivery systems are of much interest since they can be produced by simple low cost and solvent-free procedures. Pharmacosomes are supramolecular-structured nanocarriers with benefits for drug stability and targeting delivery. Amphotericin B (AmB) still remains an important agent for the treatment of invasive mold infections, e.g invasive aspergillo-sis, although the challenge for new formulations is still prevailing due to high rates of toxicity. Objective: We have previously reported the incorporation of AmB into 12-hydroxystearic acid lipid-based microtubes (MTs) for topical use, herein we report the ability of AmB-MTs to self-assemble into vesicles upon dilution. Methods: AmB-MTs with different drug concentrations (1, 3, 5 mg/ml) were prepared, and size de-termination was carried out for different dilutions. Morphology was evaluated by microscopy. In vitro cytotoxicity was evaluated in Vero cells and in vitro activity against Aspergillus fumigatus and Asper-gillus flavus was assessed. Results: AmB-MTs closed upon dilution to form vesicles ranging from 200 nm to 1µm. AmB MIC (Minimum inhibitory concentration) for both Aspergillus species was 0.0625 and 0.125 µg/ml for dis-persion and reconstituted lyophilized, respectively. Conclusion: AmB pharmacosome-like vesicles are smaller structures than MTs may thus be favoura-ble for other delivery routes. We assume that this kind of pharmacosomes-like carrier is a promising model for the obtention of new vesicular carriers based on lipid MTs.
Fil: Salerno, Claudia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cuestas, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Manco, Karina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Chiappetta, Diego Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
AMPHOTERICIN B
DRUG DELIVERY
LIPID MICROTUBES
NANOTECHNOLOGY
PHARMACOSOME-LIKE
SELF-ASSEMBLY
VESICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151786
Acceder
id CONICETDig_ac57c47fd028c4e52ba3e8b16a097340
oai_identifier_str oai:ri.conicet.gov.ar:11336/151786
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further ExploreSalerno, ClaudiaCuestas, María LujánManco, KarinaChiappetta, Diego AndrésLucangioli, Silvia EdithAMPHOTERICIN BDRUG DELIVERYLIPID MICROTUBESNANOTECHNOLOGYPHARMACOSOME-LIKESELF-ASSEMBLYVESICLEShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Background: Self-assembled drug delivery systems are of much interest since they can be produced by simple low cost and solvent-free procedures. Pharmacosomes are supramolecular-structured nanocarriers with benefits for drug stability and targeting delivery. Amphotericin B (AmB) still remains an important agent for the treatment of invasive mold infections, e.g invasive aspergillo-sis, although the challenge for new formulations is still prevailing due to high rates of toxicity. Objective: We have previously reported the incorporation of AmB into 12-hydroxystearic acid lipid-based microtubes (MTs) for topical use, herein we report the ability of AmB-MTs to self-assemble into vesicles upon dilution. Methods: AmB-MTs with different drug concentrations (1, 3, 5 mg/ml) were prepared, and size de-termination was carried out for different dilutions. Morphology was evaluated by microscopy. In vitro cytotoxicity was evaluated in Vero cells and in vitro activity against Aspergillus fumigatus and Asper-gillus flavus was assessed. Results: AmB-MTs closed upon dilution to form vesicles ranging from 200 nm to 1µm. AmB MIC (Minimum inhibitory concentration) for both Aspergillus species was 0.0625 and 0.125 µg/ml for dis-persion and reconstituted lyophilized, respectively. Conclusion: AmB pharmacosome-like vesicles are smaller structures than MTs may thus be favoura-ble for other delivery routes. We assume that this kind of pharmacosomes-like carrier is a promising model for the obtention of new vesicular carriers based on lipid MTs.Fil: Salerno, Claudia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Cuestas, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Manco, Karina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Chiappetta, Diego Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaBentham Science Publishers2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151786Salerno, Claudia; Cuestas, María Luján; Manco, Karina; Chiappetta, Diego Andrés; Lucangioli, Silvia Edith; Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore; Bentham Science Publishers; Micro and Nanosystems; 13; 3; 7-2020; 241-2451876-40291876-4037CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.eurekaselect.com/article/108527info:eu-repo/semantics/altIdentifier/doi/10.2174/1876402912999200727172806info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:55Zoai:ri.conicet.gov.ar:11336/151786instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:55.831CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore
title Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore
spellingShingle Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore
Salerno, Claudia
AMPHOTERICIN B
DRUG DELIVERY
LIPID MICROTUBES
NANOTECHNOLOGY
PHARMACOSOME-LIKE
SELF-ASSEMBLY
VESICLES
title_short Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore
title_full Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore
title_fullStr Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore
title_full_unstemmed Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore
title_sort Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore
dc.creator.none.fl_str_mv Salerno, Claudia
Cuestas, María Luján
Manco, Karina
Chiappetta, Diego Andrés
Lucangioli, Silvia Edith
author Salerno, Claudia
author_facet Salerno, Claudia
Cuestas, María Luján
Manco, Karina
Chiappetta, Diego Andrés
Lucangioli, Silvia Edith
author_role author
author2 Cuestas, María Luján
Manco, Karina
Chiappetta, Diego Andrés
Lucangioli, Silvia Edith
author2_role author
author
author
author
dc.subject.none.fl_str_mv AMPHOTERICIN B
DRUG DELIVERY
LIPID MICROTUBES
NANOTECHNOLOGY
PHARMACOSOME-LIKE
SELF-ASSEMBLY
VESICLES
topic AMPHOTERICIN B
DRUG DELIVERY
LIPID MICROTUBES
NANOTECHNOLOGY
PHARMACOSOME-LIKE
SELF-ASSEMBLY
VESICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Background: Self-assembled drug delivery systems are of much interest since they can be produced by simple low cost and solvent-free procedures. Pharmacosomes are supramolecular-structured nanocarriers with benefits for drug stability and targeting delivery. Amphotericin B (AmB) still remains an important agent for the treatment of invasive mold infections, e.g invasive aspergillo-sis, although the challenge for new formulations is still prevailing due to high rates of toxicity. Objective: We have previously reported the incorporation of AmB into 12-hydroxystearic acid lipid-based microtubes (MTs) for topical use, herein we report the ability of AmB-MTs to self-assemble into vesicles upon dilution. Methods: AmB-MTs with different drug concentrations (1, 3, 5 mg/ml) were prepared, and size de-termination was carried out for different dilutions. Morphology was evaluated by microscopy. In vitro cytotoxicity was evaluated in Vero cells and in vitro activity against Aspergillus fumigatus and Asper-gillus flavus was assessed. Results: AmB-MTs closed upon dilution to form vesicles ranging from 200 nm to 1µm. AmB MIC (Minimum inhibitory concentration) for both Aspergillus species was 0.0625 and 0.125 µg/ml for dis-persion and reconstituted lyophilized, respectively. Conclusion: AmB pharmacosome-like vesicles are smaller structures than MTs may thus be favoura-ble for other delivery routes. We assume that this kind of pharmacosomes-like carrier is a promising model for the obtention of new vesicular carriers based on lipid MTs.
Fil: Salerno, Claudia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Cuestas, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Manco, Karina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Chiappetta, Diego Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Background: Self-assembled drug delivery systems are of much interest since they can be produced by simple low cost and solvent-free procedures. Pharmacosomes are supramolecular-structured nanocarriers with benefits for drug stability and targeting delivery. Amphotericin B (AmB) still remains an important agent for the treatment of invasive mold infections, e.g invasive aspergillo-sis, although the challenge for new formulations is still prevailing due to high rates of toxicity. Objective: We have previously reported the incorporation of AmB into 12-hydroxystearic acid lipid-based microtubes (MTs) for topical use, herein we report the ability of AmB-MTs to self-assemble into vesicles upon dilution. Methods: AmB-MTs with different drug concentrations (1, 3, 5 mg/ml) were prepared, and size de-termination was carried out for different dilutions. Morphology was evaluated by microscopy. In vitro cytotoxicity was evaluated in Vero cells and in vitro activity against Aspergillus fumigatus and Asper-gillus flavus was assessed. Results: AmB-MTs closed upon dilution to form vesicles ranging from 200 nm to 1µm. AmB MIC (Minimum inhibitory concentration) for both Aspergillus species was 0.0625 and 0.125 µg/ml for dis-persion and reconstituted lyophilized, respectively. Conclusion: AmB pharmacosome-like vesicles are smaller structures than MTs may thus be favoura-ble for other delivery routes. We assume that this kind of pharmacosomes-like carrier is a promising model for the obtention of new vesicular carriers based on lipid MTs.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151786
Salerno, Claudia; Cuestas, María Luján; Manco, Karina; Chiappetta, Diego Andrés; Lucangioli, Silvia Edith; Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore; Bentham Science Publishers; Micro and Nanosystems; 13; 3; 7-2020; 241-245
1876-4029
1876-4037
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151786
identifier_str_mv Salerno, Claudia; Cuestas, María Luján; Manco, Karina; Chiappetta, Diego Andrés; Lucangioli, Silvia Edith; Self-assembled Amphotericin B Pharmacosome like Vesicles Derived from Lipid-based Microtubes: a Model Carrier to Further Explore; Bentham Science Publishers; Micro and Nanosystems; 13; 3; 7-2020; 241-245
1876-4029
1876-4037
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.eurekaselect.com/article/108527
info:eu-repo/semantics/altIdentifier/doi/10.2174/1876402912999200727172806
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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