Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse

Autores
Vattino, Lucas Gabriel; Wedemeyer, Carolina; Elgoyhen, Ana Belen; Katz, Eleonora
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The organ of Corti, the auditory mammalian sensory epithelium, contains two types of mechanotransducer cells, inner and outer hair cells (IHCs and OHCs, respectively). IHCs are involved in conveying acoustic stimuli to the central nervous system while OHCs are implicated in the fine tuning and amplification of sounds. OHCs are innervated by medial olivocochlear (MOC) cholinergic efferent fibers. The functional characteristics of the MOC-OHC synapse during maturation were assessed by electrophysiological and pharmacological methods in mouse organs of Corti at postnatal days (P)11-13, hearing onset in altricial rodents, and at P20-22 when the OHCs are morphologically and functionally mature. Synaptic currents were recorded in whole-cell voltage-clamped OHCs while electrically stimulating the MOC fibers. A progressive increase in the number of functional MOC-OHC synapses, as well as in their strength and efficacy was observed between P11-13 and P20-22. At hearing onset, the MOC-OHC synapse presented facilitation upon MOC fibers high-frequency stimulation which disappeared at mature stages. In addition, important changes were found in the VGCC that are coupled to transmitter release. Ca2+ flowing in through L-type VGCCs contribute to trigger ACh release together with P/Q- and R-type VGCCs at P11-13 but not at P20-22. Interestingly, N-type VGCCs were found to be involved in this process at P20-22 but not at hearing onset. Moreover, the degree of compartmentalization of calcium channels with respect to BK channels and presynaptic release components significantly increased from P11-13 to P20-22. These results suggest that the MOC-OHC synapse is immature at the onset of hearing.
Fil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Katz, Eleonora. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
OUTER HAIR CELL
EFFERENT
MATURATION
COCLEA
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/108313
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/108313
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapseVattino, Lucas GabrielWedemeyer, CarolinaElgoyhen, Ana BelenKatz, EleonoraOUTER HAIR CELLEFFERENTMATURATIONCOCLEAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The organ of Corti, the auditory mammalian sensory epithelium, contains two types of mechanotransducer cells, inner and outer hair cells (IHCs and OHCs, respectively). IHCs are involved in conveying acoustic stimuli to the central nervous system while OHCs are implicated in the fine tuning and amplification of sounds. OHCs are innervated by medial olivocochlear (MOC) cholinergic efferent fibers. The functional characteristics of the MOC-OHC synapse during maturation were assessed by electrophysiological and pharmacological methods in mouse organs of Corti at postnatal days (P)11-13, hearing onset in altricial rodents, and at P20-22 when the OHCs are morphologically and functionally mature. Synaptic currents were recorded in whole-cell voltage-clamped OHCs while electrically stimulating the MOC fibers. A progressive increase in the number of functional MOC-OHC synapses, as well as in their strength and efficacy was observed between P11-13 and P20-22. At hearing onset, the MOC-OHC synapse presented facilitation upon MOC fibers high-frequency stimulation which disappeared at mature stages. In addition, important changes were found in the VGCC that are coupled to transmitter release. Ca2+ flowing in through L-type VGCCs contribute to trigger ACh release together with P/Q- and R-type VGCCs at P11-13 but not at P20-22. Interestingly, N-type VGCCs were found to be involved in this process at P20-22 but not at hearing onset. Moreover, the degree of compartmentalization of calcium channels with respect to BK channels and presynaptic release components significantly increased from P11-13 to P20-22. These results suggest that the MOC-OHC synapse is immature at the onset of hearing.Fil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; ArgentinaFil: Katz, Eleonora. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaSociety for Neuroscience2020-06-17info:eu-repo/date/embargoEnd/2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/108313Vattino, Lucas Gabriel; Wedemeyer, Carolina; Elgoyhen, Ana Belen; Katz, Eleonora; Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse; Society for Neuroscience; Journal of Neuroscience; 40; 25; 17-6-2020; 4842-48570270-64741529-2401CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.2409-19.2020info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.2409-19.2020info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:09Zoai:ri.conicet.gov.ar:11336/108313instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:09.919CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse
title Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse
spellingShingle Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse
Vattino, Lucas Gabriel
OUTER HAIR CELL
EFFERENT
MATURATION
COCLEA
title_short Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse
title_full Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse
title_fullStr Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse
title_full_unstemmed Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse
title_sort Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse
dc.creator.none.fl_str_mv Vattino, Lucas Gabriel
Wedemeyer, Carolina
Elgoyhen, Ana Belen
Katz, Eleonora
author Vattino, Lucas Gabriel
author_facet Vattino, Lucas Gabriel
Wedemeyer, Carolina
Elgoyhen, Ana Belen
Katz, Eleonora
author_role author
author2 Wedemeyer, Carolina
Elgoyhen, Ana Belen
Katz, Eleonora
author2_role author
author
author
dc.subject.none.fl_str_mv OUTER HAIR CELL
EFFERENT
MATURATION
COCLEA
topic OUTER HAIR CELL
EFFERENT
MATURATION
COCLEA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The organ of Corti, the auditory mammalian sensory epithelium, contains two types of mechanotransducer cells, inner and outer hair cells (IHCs and OHCs, respectively). IHCs are involved in conveying acoustic stimuli to the central nervous system while OHCs are implicated in the fine tuning and amplification of sounds. OHCs are innervated by medial olivocochlear (MOC) cholinergic efferent fibers. The functional characteristics of the MOC-OHC synapse during maturation were assessed by electrophysiological and pharmacological methods in mouse organs of Corti at postnatal days (P)11-13, hearing onset in altricial rodents, and at P20-22 when the OHCs are morphologically and functionally mature. Synaptic currents were recorded in whole-cell voltage-clamped OHCs while electrically stimulating the MOC fibers. A progressive increase in the number of functional MOC-OHC synapses, as well as in their strength and efficacy was observed between P11-13 and P20-22. At hearing onset, the MOC-OHC synapse presented facilitation upon MOC fibers high-frequency stimulation which disappeared at mature stages. In addition, important changes were found in the VGCC that are coupled to transmitter release. Ca2+ flowing in through L-type VGCCs contribute to trigger ACh release together with P/Q- and R-type VGCCs at P11-13 but not at P20-22. Interestingly, N-type VGCCs were found to be involved in this process at P20-22 but not at hearing onset. Moreover, the degree of compartmentalization of calcium channels with respect to BK channels and presynaptic release components significantly increased from P11-13 to P20-22. These results suggest that the MOC-OHC synapse is immature at the onset of hearing.
Fil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
Fil: Katz, Eleonora. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description The organ of Corti, the auditory mammalian sensory epithelium, contains two types of mechanotransducer cells, inner and outer hair cells (IHCs and OHCs, respectively). IHCs are involved in conveying acoustic stimuli to the central nervous system while OHCs are implicated in the fine tuning and amplification of sounds. OHCs are innervated by medial olivocochlear (MOC) cholinergic efferent fibers. The functional characteristics of the MOC-OHC synapse during maturation were assessed by electrophysiological and pharmacological methods in mouse organs of Corti at postnatal days (P)11-13, hearing onset in altricial rodents, and at P20-22 when the OHCs are morphologically and functionally mature. Synaptic currents were recorded in whole-cell voltage-clamped OHCs while electrically stimulating the MOC fibers. A progressive increase in the number of functional MOC-OHC synapses, as well as in their strength and efficacy was observed between P11-13 and P20-22. At hearing onset, the MOC-OHC synapse presented facilitation upon MOC fibers high-frequency stimulation which disappeared at mature stages. In addition, important changes were found in the VGCC that are coupled to transmitter release. Ca2+ flowing in through L-type VGCCs contribute to trigger ACh release together with P/Q- and R-type VGCCs at P11-13 but not at P20-22. Interestingly, N-type VGCCs were found to be involved in this process at P20-22 but not at hearing onset. Moreover, the degree of compartmentalization of calcium channels with respect to BK channels and presynaptic release components significantly increased from P11-13 to P20-22. These results suggest that the MOC-OHC synapse is immature at the onset of hearing.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-17
info:eu-repo/date/embargoEnd/2021-01-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/108313
Vattino, Lucas Gabriel; Wedemeyer, Carolina; Elgoyhen, Ana Belen; Katz, Eleonora; Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse; Society for Neuroscience; Journal of Neuroscience; 40; 25; 17-6-2020; 4842-4857
0270-6474
1529-2401
CONICET Digital
CONICET
url http://hdl.handle.net/11336/108313
identifier_str_mv Vattino, Lucas Gabriel; Wedemeyer, Carolina; Elgoyhen, Ana Belen; Katz, Eleonora; Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse; Society for Neuroscience; Journal of Neuroscience; 40; 25; 17-6-2020; 4842-4857
0270-6474
1529-2401
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.2409-19.2020
info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.2409-19.2020
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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