Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia
- Autores
- Kip, N.; Fritz, C.; Langelaan, E.S.; Pan, Y.; Bodrossy, L.; Pancotto, Veronica Andrea; Jetten, M. S. M.; Smolders, A. J. P.; Op Den Camp, H. J. M.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Sphagnum peatlands are important ecosystems in the methane cycle. Methanotrophs living inside the dead hyaline cells or on the Sphagnum mosses are able to act as a methane filter and thereby reduce methane emissions. We investigated in situ methane concentrations and the corresponding activity and diversity of methanotrophs in different Sphagnum dominated bog microhabitats. In contrast to the Northern Hemisphere peat ecosystems the temperate South American peat bogs are dominated by one moss species; Sphagnum magellanicum. This permitted a species-independent comparison of the different bog microhabitats. Potential methane oxidizing activity was found in all Sphagnum mosses sampled and a positive correlation was found between activity and in situ methane concentrations. Substantial methane oxidation activity (23 μmol CH 4 gDW -1 day -1) was found in pool mosses and could be correlated with higher in situ methane concentrations (>35 μmol CH 4 l -1 pore water). Little methanotrophic activity (<0.5 μmol CH 4 gDW -1 day -1) was observed in living Sphagnum mosses from lawns and hummocks. Methane oxidation activity was relatively high (>4 μmol CH 4 gDW -1 day -1) in Sphagnum litter at depths around the water levels and rich in methane. The total bacterial community was studied using 16S rRNA gene sequencing and the methanotrophic communities were studied using a pmoA microarray and a complementary pmoA clone library. The methanotrophic diversity was similar in the different habitats of this study and comparable to the methanotrophic diversity found in peat mosses from the Northern Hemisphere. The pmoA microarray data indicated that both alpha- and gammaproteobacterial methanotrophs were present in all Sphagnum mosses, even in those mosses with a low initial methane oxidation activity. Prolonged incubation of Sphagnum mosses from lawn and hummock with methane revealed that the methanotrophic community present was viable and showed an increased activity within 15 days. The high abundance of methanotrophic Methylocystis species in the most active mosses suggests that these might be responsible for the bulk of methane oxidation.
Fil: Kip, N.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Fritz, C.. Radboud Universiteit Nijmegen; Países Bajos. University Of Groningen; Países Bajos. University of Groningen; Países Bajos
Fil: Langelaan, E.S.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Pan, Y.. Austrian Institute Of Technology; Austria
Fil: Bodrossy, L.. Austrian Institute Of Technology; Austria. Commonwealth Scientific And Industrial Research Organization; Australia
Fil: Pancotto, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Jetten, M. S. M.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Smolders, A. J. P.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Op Den Camp, H. J. M.. Radboud Universiteit Nijmegen; Países Bajos - Materia
-
METHANOTROPHIC ACTIVITY
PEATBOGS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/94955
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Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of PatagoniaKip, N.Fritz, C.Langelaan, E.S.Pan, Y.Bodrossy, L.Pancotto, Veronica AndreaJetten, M. S. M.Smolders, A. J. P.Op Den Camp, H. J. M.METHANOTROPHIC ACTIVITYPEATBOGShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sphagnum peatlands are important ecosystems in the methane cycle. Methanotrophs living inside the dead hyaline cells or on the Sphagnum mosses are able to act as a methane filter and thereby reduce methane emissions. We investigated in situ methane concentrations and the corresponding activity and diversity of methanotrophs in different Sphagnum dominated bog microhabitats. In contrast to the Northern Hemisphere peat ecosystems the temperate South American peat bogs are dominated by one moss species; Sphagnum magellanicum. This permitted a species-independent comparison of the different bog microhabitats. Potential methane oxidizing activity was found in all Sphagnum mosses sampled and a positive correlation was found between activity and in situ methane concentrations. Substantial methane oxidation activity (23 μmol CH 4 gDW -1 day -1) was found in pool mosses and could be correlated with higher in situ methane concentrations (>35 μmol CH 4 l -1 pore water). Little methanotrophic activity (<0.5 μmol CH 4 gDW -1 day -1) was observed in living Sphagnum mosses from lawns and hummocks. Methane oxidation activity was relatively high (>4 μmol CH 4 gDW -1 day -1) in Sphagnum litter at depths around the water levels and rich in methane. The total bacterial community was studied using 16S rRNA gene sequencing and the methanotrophic communities were studied using a pmoA microarray and a complementary pmoA clone library. The methanotrophic diversity was similar in the different habitats of this study and comparable to the methanotrophic diversity found in peat mosses from the Northern Hemisphere. The pmoA microarray data indicated that both alpha- and gammaproteobacterial methanotrophs were present in all Sphagnum mosses, even in those mosses with a low initial methane oxidation activity. Prolonged incubation of Sphagnum mosses from lawn and hummock with methane revealed that the methanotrophic community present was viable and showed an increased activity within 15 days. The high abundance of methanotrophic Methylocystis species in the most active mosses suggests that these might be responsible for the bulk of methane oxidation.Fil: Kip, N.. Radboud Universiteit Nijmegen; Países BajosFil: Fritz, C.. Radboud Universiteit Nijmegen; Países Bajos. University Of Groningen; Países Bajos. University of Groningen; Países BajosFil: Langelaan, E.S.. Radboud Universiteit Nijmegen; Países BajosFil: Pan, Y.. Austrian Institute Of Technology; AustriaFil: Bodrossy, L.. Austrian Institute Of Technology; Austria. Commonwealth Scientific And Industrial Research Organization; AustraliaFil: Pancotto, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Jetten, M. S. M.. Radboud Universiteit Nijmegen; Países BajosFil: Smolders, A. J. P.. Radboud Universiteit Nijmegen; Países BajosFil: Op Den Camp, H. J. M.. Radboud Universiteit Nijmegen; Países BajosCopernicus Publications2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94955Kip, N.; Fritz, C.; Langelaan, E.S.; Pan, Y.; Bodrossy, L.; et al.; Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia; Copernicus Publications; Biogeosciences; 9; 1; 12-2012; 47-551726-41701726-4189CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.biogeosciences.net/9/47/2012/info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-9-47-2012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:39Zoai:ri.conicet.gov.ar:11336/94955instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:39.559CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia |
title |
Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia |
spellingShingle |
Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia Kip, N. METHANOTROPHIC ACTIVITY PEATBOGS |
title_short |
Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia |
title_full |
Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia |
title_fullStr |
Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia |
title_full_unstemmed |
Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia |
title_sort |
Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia |
dc.creator.none.fl_str_mv |
Kip, N. Fritz, C. Langelaan, E.S. Pan, Y. Bodrossy, L. Pancotto, Veronica Andrea Jetten, M. S. M. Smolders, A. J. P. Op Den Camp, H. J. M. |
author |
Kip, N. |
author_facet |
Kip, N. Fritz, C. Langelaan, E.S. Pan, Y. Bodrossy, L. Pancotto, Veronica Andrea Jetten, M. S. M. Smolders, A. J. P. Op Den Camp, H. J. M. |
author_role |
author |
author2 |
Fritz, C. Langelaan, E.S. Pan, Y. Bodrossy, L. Pancotto, Veronica Andrea Jetten, M. S. M. Smolders, A. J. P. Op Den Camp, H. J. M. |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
METHANOTROPHIC ACTIVITY PEATBOGS |
topic |
METHANOTROPHIC ACTIVITY PEATBOGS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Sphagnum peatlands are important ecosystems in the methane cycle. Methanotrophs living inside the dead hyaline cells or on the Sphagnum mosses are able to act as a methane filter and thereby reduce methane emissions. We investigated in situ methane concentrations and the corresponding activity and diversity of methanotrophs in different Sphagnum dominated bog microhabitats. In contrast to the Northern Hemisphere peat ecosystems the temperate South American peat bogs are dominated by one moss species; Sphagnum magellanicum. This permitted a species-independent comparison of the different bog microhabitats. Potential methane oxidizing activity was found in all Sphagnum mosses sampled and a positive correlation was found between activity and in situ methane concentrations. Substantial methane oxidation activity (23 μmol CH 4 gDW -1 day -1) was found in pool mosses and could be correlated with higher in situ methane concentrations (>35 μmol CH 4 l -1 pore water). Little methanotrophic activity (<0.5 μmol CH 4 gDW -1 day -1) was observed in living Sphagnum mosses from lawns and hummocks. Methane oxidation activity was relatively high (>4 μmol CH 4 gDW -1 day -1) in Sphagnum litter at depths around the water levels and rich in methane. The total bacterial community was studied using 16S rRNA gene sequencing and the methanotrophic communities were studied using a pmoA microarray and a complementary pmoA clone library. The methanotrophic diversity was similar in the different habitats of this study and comparable to the methanotrophic diversity found in peat mosses from the Northern Hemisphere. The pmoA microarray data indicated that both alpha- and gammaproteobacterial methanotrophs were present in all Sphagnum mosses, even in those mosses with a low initial methane oxidation activity. Prolonged incubation of Sphagnum mosses from lawn and hummock with methane revealed that the methanotrophic community present was viable and showed an increased activity within 15 days. The high abundance of methanotrophic Methylocystis species in the most active mosses suggests that these might be responsible for the bulk of methane oxidation. Fil: Kip, N.. Radboud Universiteit Nijmegen; Países Bajos Fil: Fritz, C.. Radboud Universiteit Nijmegen; Países Bajos. University Of Groningen; Países Bajos. University of Groningen; Países Bajos Fil: Langelaan, E.S.. Radboud Universiteit Nijmegen; Países Bajos Fil: Pan, Y.. Austrian Institute Of Technology; Austria Fil: Bodrossy, L.. Austrian Institute Of Technology; Austria. Commonwealth Scientific And Industrial Research Organization; Australia Fil: Pancotto, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina Fil: Jetten, M. S. M.. Radboud Universiteit Nijmegen; Países Bajos Fil: Smolders, A. J. P.. Radboud Universiteit Nijmegen; Países Bajos Fil: Op Den Camp, H. J. M.. Radboud Universiteit Nijmegen; Países Bajos |
description |
Sphagnum peatlands are important ecosystems in the methane cycle. Methanotrophs living inside the dead hyaline cells or on the Sphagnum mosses are able to act as a methane filter and thereby reduce methane emissions. We investigated in situ methane concentrations and the corresponding activity and diversity of methanotrophs in different Sphagnum dominated bog microhabitats. In contrast to the Northern Hemisphere peat ecosystems the temperate South American peat bogs are dominated by one moss species; Sphagnum magellanicum. This permitted a species-independent comparison of the different bog microhabitats. Potential methane oxidizing activity was found in all Sphagnum mosses sampled and a positive correlation was found between activity and in situ methane concentrations. Substantial methane oxidation activity (23 μmol CH 4 gDW -1 day -1) was found in pool mosses and could be correlated with higher in situ methane concentrations (>35 μmol CH 4 l -1 pore water). Little methanotrophic activity (<0.5 μmol CH 4 gDW -1 day -1) was observed in living Sphagnum mosses from lawns and hummocks. Methane oxidation activity was relatively high (>4 μmol CH 4 gDW -1 day -1) in Sphagnum litter at depths around the water levels and rich in methane. The total bacterial community was studied using 16S rRNA gene sequencing and the methanotrophic communities were studied using a pmoA microarray and a complementary pmoA clone library. The methanotrophic diversity was similar in the different habitats of this study and comparable to the methanotrophic diversity found in peat mosses from the Northern Hemisphere. The pmoA microarray data indicated that both alpha- and gammaproteobacterial methanotrophs were present in all Sphagnum mosses, even in those mosses with a low initial methane oxidation activity. Prolonged incubation of Sphagnum mosses from lawn and hummock with methane revealed that the methanotrophic community present was viable and showed an increased activity within 15 days. The high abundance of methanotrophic Methylocystis species in the most active mosses suggests that these might be responsible for the bulk of methane oxidation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/94955 Kip, N.; Fritz, C.; Langelaan, E.S.; Pan, Y.; Bodrossy, L.; et al.; Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia; Copernicus Publications; Biogeosciences; 9; 1; 12-2012; 47-55 1726-4170 1726-4189 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/94955 |
identifier_str_mv |
Kip, N.; Fritz, C.; Langelaan, E.S.; Pan, Y.; Bodrossy, L.; et al.; Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia; Copernicus Publications; Biogeosciences; 9; 1; 12-2012; 47-55 1726-4170 1726-4189 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.biogeosciences.net/9/47/2012/ info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-9-47-2012 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Copernicus Publications |
publisher.none.fl_str_mv |
Copernicus Publications |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613917576265728 |
score |
13.070432 |