Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia

Autores
Kip, N.; Fritz, C.; Langelaan, E.S.; Pan, Y.; Bodrossy, L.; Pancotto, Veronica Andrea; Jetten, M. S. M.; Smolders, A. J. P.; Op Den Camp, H. J. M.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sphagnum peatlands are important ecosystems in the methane cycle. Methanotrophs living inside the dead hyaline cells or on the Sphagnum mosses are able to act as a methane filter and thereby reduce methane emissions. We investigated in situ methane concentrations and the corresponding activity and diversity of methanotrophs in different Sphagnum dominated bog microhabitats. In contrast to the Northern Hemisphere peat ecosystems the temperate South American peat bogs are dominated by one moss species; Sphagnum magellanicum. This permitted a species-independent comparison of the different bog microhabitats. Potential methane oxidizing activity was found in all Sphagnum mosses sampled and a positive correlation was found between activity and in situ methane concentrations. Substantial methane oxidation activity (23 μmol CH 4 gDW -1 day -1) was found in pool mosses and could be correlated with higher in situ methane concentrations (>35 μmol CH 4 l -1 pore water). Little methanotrophic activity (<0.5 μmol CH 4 gDW -1 day -1) was observed in living Sphagnum mosses from lawns and hummocks. Methane oxidation activity was relatively high (>4 μmol CH 4 gDW -1 day -1) in Sphagnum litter at depths around the water levels and rich in methane. The total bacterial community was studied using 16S rRNA gene sequencing and the methanotrophic communities were studied using a pmoA microarray and a complementary pmoA clone library. The methanotrophic diversity was similar in the different habitats of this study and comparable to the methanotrophic diversity found in peat mosses from the Northern Hemisphere. The pmoA microarray data indicated that both alpha- and gammaproteobacterial methanotrophs were present in all Sphagnum mosses, even in those mosses with a low initial methane oxidation activity. Prolonged incubation of Sphagnum mosses from lawn and hummock with methane revealed that the methanotrophic community present was viable and showed an increased activity within 15 days. The high abundance of methanotrophic Methylocystis species in the most active mosses suggests that these might be responsible for the bulk of methane oxidation.
Fil: Kip, N.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Fritz, C.. Radboud Universiteit Nijmegen; Países Bajos. University Of Groningen; Países Bajos. University of Groningen; Países Bajos
Fil: Langelaan, E.S.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Pan, Y.. Austrian Institute Of Technology; Austria
Fil: Bodrossy, L.. Austrian Institute Of Technology; Austria. Commonwealth Scientific And Industrial Research Organization; Australia
Fil: Pancotto, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Jetten, M. S. M.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Smolders, A. J. P.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Op Den Camp, H. J. M.. Radboud Universiteit Nijmegen; Países Bajos
Materia
METHANOTROPHIC ACTIVITY
PEATBOGS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94955

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network_name_str CONICET Digital (CONICET)
spelling Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of PatagoniaKip, N.Fritz, C.Langelaan, E.S.Pan, Y.Bodrossy, L.Pancotto, Veronica AndreaJetten, M. S. M.Smolders, A. J. P.Op Den Camp, H. J. M.METHANOTROPHIC ACTIVITYPEATBOGShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sphagnum peatlands are important ecosystems in the methane cycle. Methanotrophs living inside the dead hyaline cells or on the Sphagnum mosses are able to act as a methane filter and thereby reduce methane emissions. We investigated in situ methane concentrations and the corresponding activity and diversity of methanotrophs in different Sphagnum dominated bog microhabitats. In contrast to the Northern Hemisphere peat ecosystems the temperate South American peat bogs are dominated by one moss species; Sphagnum magellanicum. This permitted a species-independent comparison of the different bog microhabitats. Potential methane oxidizing activity was found in all Sphagnum mosses sampled and a positive correlation was found between activity and in situ methane concentrations. Substantial methane oxidation activity (23 μmol CH 4 gDW -1 day -1) was found in pool mosses and could be correlated with higher in situ methane concentrations (>35 μmol CH 4 l -1 pore water). Little methanotrophic activity (<0.5 μmol CH 4 gDW -1 day -1) was observed in living Sphagnum mosses from lawns and hummocks. Methane oxidation activity was relatively high (>4 μmol CH 4 gDW -1 day -1) in Sphagnum litter at depths around the water levels and rich in methane. The total bacterial community was studied using 16S rRNA gene sequencing and the methanotrophic communities were studied using a pmoA microarray and a complementary pmoA clone library. The methanotrophic diversity was similar in the different habitats of this study and comparable to the methanotrophic diversity found in peat mosses from the Northern Hemisphere. The pmoA microarray data indicated that both alpha- and gammaproteobacterial methanotrophs were present in all Sphagnum mosses, even in those mosses with a low initial methane oxidation activity. Prolonged incubation of Sphagnum mosses from lawn and hummock with methane revealed that the methanotrophic community present was viable and showed an increased activity within 15 days. The high abundance of methanotrophic Methylocystis species in the most active mosses suggests that these might be responsible for the bulk of methane oxidation.Fil: Kip, N.. Radboud Universiteit Nijmegen; Países BajosFil: Fritz, C.. Radboud Universiteit Nijmegen; Países Bajos. University Of Groningen; Países Bajos. University of Groningen; Países BajosFil: Langelaan, E.S.. Radboud Universiteit Nijmegen; Países BajosFil: Pan, Y.. Austrian Institute Of Technology; AustriaFil: Bodrossy, L.. Austrian Institute Of Technology; Austria. Commonwealth Scientific And Industrial Research Organization; AustraliaFil: Pancotto, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Jetten, M. S. M.. Radboud Universiteit Nijmegen; Países BajosFil: Smolders, A. J. P.. Radboud Universiteit Nijmegen; Países BajosFil: Op Den Camp, H. J. M.. Radboud Universiteit Nijmegen; Países BajosCopernicus Publications2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94955Kip, N.; Fritz, C.; Langelaan, E.S.; Pan, Y.; Bodrossy, L.; et al.; Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia; Copernicus Publications; Biogeosciences; 9; 1; 12-2012; 47-551726-41701726-4189CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.biogeosciences.net/9/47/2012/info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-9-47-2012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:39Zoai:ri.conicet.gov.ar:11336/94955instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:39.559CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia
title Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia
spellingShingle Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia
Kip, N.
METHANOTROPHIC ACTIVITY
PEATBOGS
title_short Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia
title_full Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia
title_fullStr Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia
title_full_unstemmed Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia
title_sort Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia
dc.creator.none.fl_str_mv Kip, N.
Fritz, C.
Langelaan, E.S.
Pan, Y.
Bodrossy, L.
Pancotto, Veronica Andrea
Jetten, M. S. M.
Smolders, A. J. P.
Op Den Camp, H. J. M.
author Kip, N.
author_facet Kip, N.
Fritz, C.
Langelaan, E.S.
Pan, Y.
Bodrossy, L.
Pancotto, Veronica Andrea
Jetten, M. S. M.
Smolders, A. J. P.
Op Den Camp, H. J. M.
author_role author
author2 Fritz, C.
Langelaan, E.S.
Pan, Y.
Bodrossy, L.
Pancotto, Veronica Andrea
Jetten, M. S. M.
Smolders, A. J. P.
Op Den Camp, H. J. M.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv METHANOTROPHIC ACTIVITY
PEATBOGS
topic METHANOTROPHIC ACTIVITY
PEATBOGS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sphagnum peatlands are important ecosystems in the methane cycle. Methanotrophs living inside the dead hyaline cells or on the Sphagnum mosses are able to act as a methane filter and thereby reduce methane emissions. We investigated in situ methane concentrations and the corresponding activity and diversity of methanotrophs in different Sphagnum dominated bog microhabitats. In contrast to the Northern Hemisphere peat ecosystems the temperate South American peat bogs are dominated by one moss species; Sphagnum magellanicum. This permitted a species-independent comparison of the different bog microhabitats. Potential methane oxidizing activity was found in all Sphagnum mosses sampled and a positive correlation was found between activity and in situ methane concentrations. Substantial methane oxidation activity (23 μmol CH 4 gDW -1 day -1) was found in pool mosses and could be correlated with higher in situ methane concentrations (>35 μmol CH 4 l -1 pore water). Little methanotrophic activity (<0.5 μmol CH 4 gDW -1 day -1) was observed in living Sphagnum mosses from lawns and hummocks. Methane oxidation activity was relatively high (>4 μmol CH 4 gDW -1 day -1) in Sphagnum litter at depths around the water levels and rich in methane. The total bacterial community was studied using 16S rRNA gene sequencing and the methanotrophic communities were studied using a pmoA microarray and a complementary pmoA clone library. The methanotrophic diversity was similar in the different habitats of this study and comparable to the methanotrophic diversity found in peat mosses from the Northern Hemisphere. The pmoA microarray data indicated that both alpha- and gammaproteobacterial methanotrophs were present in all Sphagnum mosses, even in those mosses with a low initial methane oxidation activity. Prolonged incubation of Sphagnum mosses from lawn and hummock with methane revealed that the methanotrophic community present was viable and showed an increased activity within 15 days. The high abundance of methanotrophic Methylocystis species in the most active mosses suggests that these might be responsible for the bulk of methane oxidation.
Fil: Kip, N.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Fritz, C.. Radboud Universiteit Nijmegen; Países Bajos. University Of Groningen; Países Bajos. University of Groningen; Países Bajos
Fil: Langelaan, E.S.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Pan, Y.. Austrian Institute Of Technology; Austria
Fil: Bodrossy, L.. Austrian Institute Of Technology; Austria. Commonwealth Scientific And Industrial Research Organization; Australia
Fil: Pancotto, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina
Fil: Jetten, M. S. M.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Smolders, A. J. P.. Radboud Universiteit Nijmegen; Países Bajos
Fil: Op Den Camp, H. J. M.. Radboud Universiteit Nijmegen; Países Bajos
description Sphagnum peatlands are important ecosystems in the methane cycle. Methanotrophs living inside the dead hyaline cells or on the Sphagnum mosses are able to act as a methane filter and thereby reduce methane emissions. We investigated in situ methane concentrations and the corresponding activity and diversity of methanotrophs in different Sphagnum dominated bog microhabitats. In contrast to the Northern Hemisphere peat ecosystems the temperate South American peat bogs are dominated by one moss species; Sphagnum magellanicum. This permitted a species-independent comparison of the different bog microhabitats. Potential methane oxidizing activity was found in all Sphagnum mosses sampled and a positive correlation was found between activity and in situ methane concentrations. Substantial methane oxidation activity (23 μmol CH 4 gDW -1 day -1) was found in pool mosses and could be correlated with higher in situ methane concentrations (>35 μmol CH 4 l -1 pore water). Little methanotrophic activity (<0.5 μmol CH 4 gDW -1 day -1) was observed in living Sphagnum mosses from lawns and hummocks. Methane oxidation activity was relatively high (>4 μmol CH 4 gDW -1 day -1) in Sphagnum litter at depths around the water levels and rich in methane. The total bacterial community was studied using 16S rRNA gene sequencing and the methanotrophic communities were studied using a pmoA microarray and a complementary pmoA clone library. The methanotrophic diversity was similar in the different habitats of this study and comparable to the methanotrophic diversity found in peat mosses from the Northern Hemisphere. The pmoA microarray data indicated that both alpha- and gammaproteobacterial methanotrophs were present in all Sphagnum mosses, even in those mosses with a low initial methane oxidation activity. Prolonged incubation of Sphagnum mosses from lawn and hummock with methane revealed that the methanotrophic community present was viable and showed an increased activity within 15 days. The high abundance of methanotrophic Methylocystis species in the most active mosses suggests that these might be responsible for the bulk of methane oxidation.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94955
Kip, N.; Fritz, C.; Langelaan, E.S.; Pan, Y.; Bodrossy, L.; et al.; Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia; Copernicus Publications; Biogeosciences; 9; 1; 12-2012; 47-55
1726-4170
1726-4189
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94955
identifier_str_mv Kip, N.; Fritz, C.; Langelaan, E.S.; Pan, Y.; Bodrossy, L.; et al.; Methanotrophic activity and diversity in different Sphagnum magellanicum dominated habitats in the southernmost peat bogs of Patagonia; Copernicus Publications; Biogeosciences; 9; 1; 12-2012; 47-55
1726-4170
1726-4189
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.biogeosciences.net/9/47/2012/
info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-9-47-2012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Copernicus Publications
publisher.none.fl_str_mv Copernicus Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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