Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica

Autores
Torres, María Julia; Flores Rocha, Voyko; Audisio, Marcela Carina
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The house fly, Musca domestica L. (Diptera: Muscidae), is a key problem in animal producing and rearing areas. Currently, the use and abuse of chemical pest-control compounds has generated resistance in M. domestica and, hence, new approaches are required. In this work, the potential entomopathogenic activity of Lactobacillus johnsonii Fujisawa et al. CRL1647 was evaluated against M. domestica, under laboratory conditions. Bioassays were done for three consecutive years using bacterial cell suspensions (CS) and cell-free supernatants (CFS) under controlled conditions (27 ± 1 °C, 57 ± 2% r.h., and L12:D12 photoperiod). Both the CS and CFS displayed high levels of larvicidal (96%) and pupicidal (97%) activities. Chemical characterization of the CFS revealed that the bioactive metabolites are acidic compounds, not affected by thermal treatment or by trypsin. Organic acids were identified and quantified by high-performance liquid chromatography (HPLC); only lactic acid (129.7 ± 1.0 mM), acetic acid (37.3 ± 0.8 mM), and phenyllactic acid (0.3 ± 0.1 mM) were detected. A significant decrease in fecundity of the house flies was observed in females from larvae fed on CFS; male fertility was not affected. Interestingly, only the mixture of organic acids exerted the biological effects. These results suggest that the metabolites synthesized by L. johnsonii CRL1647 can be used in a novel biocontrol strategy against house flies.
Fil: Torres, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Flores Rocha, Voyko. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Materia
Animal Production
Diptera
Enthomopathogenic Activity
House Fly
Larvicidal Activity
Muscidae
Organic Acids
Pupicidal Activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61714
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/61714
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domesticaTorres, María JuliaFlores Rocha, VoykoAudisio, Marcela CarinaAnimal ProductionDipteraEnthomopathogenic ActivityHouse FlyLarvicidal ActivityMuscidaeOrganic AcidsPupicidal Activityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The house fly, Musca domestica L. (Diptera: Muscidae), is a key problem in animal producing and rearing areas. Currently, the use and abuse of chemical pest-control compounds has generated resistance in M. domestica and, hence, new approaches are required. In this work, the potential entomopathogenic activity of Lactobacillus johnsonii Fujisawa et al. CRL1647 was evaluated against M. domestica, under laboratory conditions. Bioassays were done for three consecutive years using bacterial cell suspensions (CS) and cell-free supernatants (CFS) under controlled conditions (27 ± 1 °C, 57 ± 2% r.h., and L12:D12 photoperiod). Both the CS and CFS displayed high levels of larvicidal (96%) and pupicidal (97%) activities. Chemical characterization of the CFS revealed that the bioactive metabolites are acidic compounds, not affected by thermal treatment or by trypsin. Organic acids were identified and quantified by high-performance liquid chromatography (HPLC); only lactic acid (129.7 ± 1.0 mM), acetic acid (37.3 ± 0.8 mM), and phenyllactic acid (0.3 ± 0.1 mM) were detected. A significant decrease in fecundity of the house flies was observed in females from larvae fed on CFS; male fertility was not affected. Interestingly, only the mixture of organic acids exerted the biological effects. These results suggest that the metabolites synthesized by L. johnsonii CRL1647 can be used in a novel biocontrol strategy against house flies.Fil: Torres, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Flores Rocha, Voyko. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaWiley Blackwell Publishing, Inc2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61714Torres, María Julia; Flores Rocha, Voyko; Audisio, Marcela Carina; Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica; Wiley Blackwell Publishing, Inc; Entomologia Experimentalis et Applicata; 159; 3; 6-2016; 347-3530013-8703CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/eea.12445info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/eea.12445info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:53Zoai:ri.conicet.gov.ar:11336/61714instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:53.961CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica
title Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica
spellingShingle Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica
Torres, María Julia
Animal Production
Diptera
Enthomopathogenic Activity
House Fly
Larvicidal Activity
Muscidae
Organic Acids
Pupicidal Activity
title_short Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica
title_full Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica
title_fullStr Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica
title_full_unstemmed Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica
title_sort Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica
dc.creator.none.fl_str_mv Torres, María Julia
Flores Rocha, Voyko
Audisio, Marcela Carina
author Torres, María Julia
author_facet Torres, María Julia
Flores Rocha, Voyko
Audisio, Marcela Carina
author_role author
author2 Flores Rocha, Voyko
Audisio, Marcela Carina
author2_role author
author
dc.subject.none.fl_str_mv Animal Production
Diptera
Enthomopathogenic Activity
House Fly
Larvicidal Activity
Muscidae
Organic Acids
Pupicidal Activity
topic Animal Production
Diptera
Enthomopathogenic Activity
House Fly
Larvicidal Activity
Muscidae
Organic Acids
Pupicidal Activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The house fly, Musca domestica L. (Diptera: Muscidae), is a key problem in animal producing and rearing areas. Currently, the use and abuse of chemical pest-control compounds has generated resistance in M. domestica and, hence, new approaches are required. In this work, the potential entomopathogenic activity of Lactobacillus johnsonii Fujisawa et al. CRL1647 was evaluated against M. domestica, under laboratory conditions. Bioassays were done for three consecutive years using bacterial cell suspensions (CS) and cell-free supernatants (CFS) under controlled conditions (27 ± 1 °C, 57 ± 2% r.h., and L12:D12 photoperiod). Both the CS and CFS displayed high levels of larvicidal (96%) and pupicidal (97%) activities. Chemical characterization of the CFS revealed that the bioactive metabolites are acidic compounds, not affected by thermal treatment or by trypsin. Organic acids were identified and quantified by high-performance liquid chromatography (HPLC); only lactic acid (129.7 ± 1.0 mM), acetic acid (37.3 ± 0.8 mM), and phenyllactic acid (0.3 ± 0.1 mM) were detected. A significant decrease in fecundity of the house flies was observed in females from larvae fed on CFS; male fertility was not affected. Interestingly, only the mixture of organic acids exerted the biological effects. These results suggest that the metabolites synthesized by L. johnsonii CRL1647 can be used in a novel biocontrol strategy against house flies.
Fil: Torres, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Fil: Flores Rocha, Voyko. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
description The house fly, Musca domestica L. (Diptera: Muscidae), is a key problem in animal producing and rearing areas. Currently, the use and abuse of chemical pest-control compounds has generated resistance in M. domestica and, hence, new approaches are required. In this work, the potential entomopathogenic activity of Lactobacillus johnsonii Fujisawa et al. CRL1647 was evaluated against M. domestica, under laboratory conditions. Bioassays were done for three consecutive years using bacterial cell suspensions (CS) and cell-free supernatants (CFS) under controlled conditions (27 ± 1 °C, 57 ± 2% r.h., and L12:D12 photoperiod). Both the CS and CFS displayed high levels of larvicidal (96%) and pupicidal (97%) activities. Chemical characterization of the CFS revealed that the bioactive metabolites are acidic compounds, not affected by thermal treatment or by trypsin. Organic acids were identified and quantified by high-performance liquid chromatography (HPLC); only lactic acid (129.7 ± 1.0 mM), acetic acid (37.3 ± 0.8 mM), and phenyllactic acid (0.3 ± 0.1 mM) were detected. A significant decrease in fecundity of the house flies was observed in females from larvae fed on CFS; male fertility was not affected. Interestingly, only the mixture of organic acids exerted the biological effects. These results suggest that the metabolites synthesized by L. johnsonii CRL1647 can be used in a novel biocontrol strategy against house flies.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61714
Torres, María Julia; Flores Rocha, Voyko; Audisio, Marcela Carina; Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica; Wiley Blackwell Publishing, Inc; Entomologia Experimentalis et Applicata; 159; 3; 6-2016; 347-353
0013-8703
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61714
identifier_str_mv Torres, María Julia; Flores Rocha, Voyko; Audisio, Marcela Carina; Laboratory evaluation of Lactobacillus johnsonii CRL1647 metabolites for biological control of Musca domestica; Wiley Blackwell Publishing, Inc; Entomologia Experimentalis et Applicata; 159; 3; 6-2016; 347-353
0013-8703
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/eea.12445
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/eea.12445
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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