A colorimetric method for the assay of ADP-glucose pyrophosphorylase

Autores
Fusari, Corina; Demonte, Ana María Magdalena; Figueroa, Carlos Maria; Aleanzi, Mabel Cristina; Iglesias, Alberto Alvaro
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of the current work was to develop a relatively simple method to assay ADPGlcPPase activity having high sensitivity, accuracy, and reliability. We sought a procedure allowing the assay in both of the reaction directions, based on a technique suitable for the screening of numerous samples. The method quantifies inorganic orthophosphate released from the specific hydrolysis of the enzyme activity products. For ADPGlc synthesis, the method measures Pi after hydrolysis of PPi by inorganic pyrophosphatase. Pyrophosphorolysis is assayed by determining Pi derived from CF1-ATPase-mediated hydrolysis of ATP. Pi dosage is performed by the technique based in the formation of a phosphomolybdate?Malachite Green complex [14,15]. We optimized the procedure to a microscale grade, reaching convenient sensitivity and the possibility of automation, by using a microplate (multiwell) absorbance reader
Fil: Fusari, Corina. Universidad Nacional del Litoral; Argentina
Fil: Demonte, Ana María Magdalena. Universidad Nacional del Litoral; Argentina
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Aleanzi, Mabel Cristina. Universidad Nacional del Litoral; Argentina
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
ADP-GLUCOSE PYROPHOSPHORYLASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85780
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A colorimetric method for the assay of ADP-glucose pyrophosphorylaseFusari, CorinaDemonte, Ana María MagdalenaFigueroa, Carlos MariaAleanzi, Mabel CristinaIglesias, Alberto AlvaroADP-GLUCOSE PYROPHOSPHORYLASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The purpose of the current work was to develop a relatively simple method to assay ADPGlcPPase activity having high sensitivity, accuracy, and reliability. We sought a procedure allowing the assay in both of the reaction directions, based on a technique suitable for the screening of numerous samples. The method quantifies inorganic orthophosphate released from the specific hydrolysis of the enzyme activity products. For ADPGlc synthesis, the method measures Pi after hydrolysis of PPi by inorganic pyrophosphatase. Pyrophosphorolysis is assayed by determining Pi derived from CF1-ATPase-mediated hydrolysis of ATP. Pi dosage is performed by the technique based in the formation of a phosphomolybdate?Malachite Green complex [14,15]. We optimized the procedure to a microscale grade, reaching convenient sensitivity and the possibility of automation, by using a microplate (multiwell) absorbance readerFil: Fusari, Corina. Universidad Nacional del Litoral; ArgentinaFil: Demonte, Ana María Magdalena. Universidad Nacional del Litoral; ArgentinaFil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Aleanzi, Mabel Cristina. Universidad Nacional del Litoral; ArgentinaFil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaAcademic Press Inc Elsevier Science2006-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85780Fusari, Corina; Demonte, Ana María Magdalena; Figueroa, Carlos Maria; Aleanzi, Mabel Cristina; Iglesias, Alberto Alvaro; A colorimetric method for the assay of ADP-glucose pyrophosphorylase; Academic Press Inc Elsevier Science; Analytical Biochemistry; 352; 1; 5-2006; 145-1470003-2697CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ab.2006.01.024info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:17Zoai:ri.conicet.gov.ar:11336/85780instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:17.503CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A colorimetric method for the assay of ADP-glucose pyrophosphorylase
title A colorimetric method for the assay of ADP-glucose pyrophosphorylase
spellingShingle A colorimetric method for the assay of ADP-glucose pyrophosphorylase
Fusari, Corina
ADP-GLUCOSE PYROPHOSPHORYLASE
title_short A colorimetric method for the assay of ADP-glucose pyrophosphorylase
title_full A colorimetric method for the assay of ADP-glucose pyrophosphorylase
title_fullStr A colorimetric method for the assay of ADP-glucose pyrophosphorylase
title_full_unstemmed A colorimetric method for the assay of ADP-glucose pyrophosphorylase
title_sort A colorimetric method for the assay of ADP-glucose pyrophosphorylase
dc.creator.none.fl_str_mv Fusari, Corina
Demonte, Ana María Magdalena
Figueroa, Carlos Maria
Aleanzi, Mabel Cristina
Iglesias, Alberto Alvaro
author Fusari, Corina
author_facet Fusari, Corina
Demonte, Ana María Magdalena
Figueroa, Carlos Maria
Aleanzi, Mabel Cristina
Iglesias, Alberto Alvaro
author_role author
author2 Demonte, Ana María Magdalena
Figueroa, Carlos Maria
Aleanzi, Mabel Cristina
Iglesias, Alberto Alvaro
author2_role author
author
author
author
dc.subject.none.fl_str_mv ADP-GLUCOSE PYROPHOSPHORYLASE
topic ADP-GLUCOSE PYROPHOSPHORYLASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The purpose of the current work was to develop a relatively simple method to assay ADPGlcPPase activity having high sensitivity, accuracy, and reliability. We sought a procedure allowing the assay in both of the reaction directions, based on a technique suitable for the screening of numerous samples. The method quantifies inorganic orthophosphate released from the specific hydrolysis of the enzyme activity products. For ADPGlc synthesis, the method measures Pi after hydrolysis of PPi by inorganic pyrophosphatase. Pyrophosphorolysis is assayed by determining Pi derived from CF1-ATPase-mediated hydrolysis of ATP. Pi dosage is performed by the technique based in the formation of a phosphomolybdate?Malachite Green complex [14,15]. We optimized the procedure to a microscale grade, reaching convenient sensitivity and the possibility of automation, by using a microplate (multiwell) absorbance reader
Fil: Fusari, Corina. Universidad Nacional del Litoral; Argentina
Fil: Demonte, Ana María Magdalena. Universidad Nacional del Litoral; Argentina
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Aleanzi, Mabel Cristina. Universidad Nacional del Litoral; Argentina
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description The purpose of the current work was to develop a relatively simple method to assay ADPGlcPPase activity having high sensitivity, accuracy, and reliability. We sought a procedure allowing the assay in both of the reaction directions, based on a technique suitable for the screening of numerous samples. The method quantifies inorganic orthophosphate released from the specific hydrolysis of the enzyme activity products. For ADPGlc synthesis, the method measures Pi after hydrolysis of PPi by inorganic pyrophosphatase. Pyrophosphorolysis is assayed by determining Pi derived from CF1-ATPase-mediated hydrolysis of ATP. Pi dosage is performed by the technique based in the formation of a phosphomolybdate?Malachite Green complex [14,15]. We optimized the procedure to a microscale grade, reaching convenient sensitivity and the possibility of automation, by using a microplate (multiwell) absorbance reader
publishDate 2006
dc.date.none.fl_str_mv 2006-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85780
Fusari, Corina; Demonte, Ana María Magdalena; Figueroa, Carlos Maria; Aleanzi, Mabel Cristina; Iglesias, Alberto Alvaro; A colorimetric method for the assay of ADP-glucose pyrophosphorylase; Academic Press Inc Elsevier Science; Analytical Biochemistry; 352; 1; 5-2006; 145-147
0003-2697
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85780
identifier_str_mv Fusari, Corina; Demonte, Ana María Magdalena; Figueroa, Carlos Maria; Aleanzi, Mabel Cristina; Iglesias, Alberto Alvaro; A colorimetric method for the assay of ADP-glucose pyrophosphorylase; Academic Press Inc Elsevier Science; Analytical Biochemistry; 352; 1; 5-2006; 145-147
0003-2697
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ab.2006.01.024
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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