Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens

Autores
Molina, Rocío Daniela Inés; Campos Silva, Rodrigo; Díaz, Myriam Anabel; Macedo, Alexandre José; Blázquez, María Amparo; Alberto, Maria Rosa; Arena, Mario Eduardo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antimicrobial, antibiofilm, anti-Quorum sensing (QS) and virulence factors inhibitory capacity of different polarity Laurus nobilis extracts against several pathogenic microorganisms were studied. Some extracts exhibited antibiotic effect against Staphylococcus aureus multidrug-resistant strains. However, all extracts (100 µg/mL) inhibited to some extent the biofilm of most bacteria tested (until 40% for Gram-negative and 76% for Gram-positive). Hexane (HE) and chloroform extract (CE) were potent inhibitors of S. aureus biofilm and the microscopies further confirmed an important reduction in adherent cells. Polystyrene surfaces coated with these extracts showed a decrease in bacterial adhesion with the resulting inhibition of S. aureus biofilm formation and biofilm activity. Moreover, they also interfere with the coagulase and hemolysin activities of this bacterium. With respect to Gram-negative bacteria, these extracts showed anti-QS activity against Chromobacterium violaceum and inhibited biofilm formation, swarming motility, pyocyanin production, and elastase activity of Pseudomonas aeruginosa. Chemical analysis of HE and CE revealed that the fatty acids were the main compounds, particularly linolenic, oleic and hexadecanoic acids followed by the oxygenated monoterpenes. Non-toxic laurel extracts offer a natural alternative to control contamination and/or spoilage of food, as well as infectious diseases, by attacking the biofilm and the virulence of Gram-positive and Gram-negative bacteria.
Fil: Molina, Rocío Daniela Inés. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina
Fil: Campos Silva, Rodrigo. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Díaz, Myriam Anabel. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina
Fil: Macedo, Alexandre José. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Blázquez, María Amparo. Universidad de Valencia; España
Fil: Alberto, Maria Rosa. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Arena, Mario Eduardo. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
BIOFILM
ENZYME INHIBITION
FATTY ACIDS
SWARMING
PYOCYANIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/143339
Acceder
id CONICETDig_aae5b8e40be43a730d13e21033a6629a
oai_identifier_str oai:ri.conicet.gov.ar:11336/143339
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogensMolina, Rocío Daniela InésCampos Silva, RodrigoDíaz, Myriam AnabelMacedo, Alexandre JoséBlázquez, María AmparoAlberto, Maria RosaArena, Mario EduardoBIOFILMENZYME INHIBITIONFATTY ACIDSSWARMINGPYOCYANINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Antimicrobial, antibiofilm, anti-Quorum sensing (QS) and virulence factors inhibitory capacity of different polarity Laurus nobilis extracts against several pathogenic microorganisms were studied. Some extracts exhibited antibiotic effect against Staphylococcus aureus multidrug-resistant strains. However, all extracts (100 µg/mL) inhibited to some extent the biofilm of most bacteria tested (until 40% for Gram-negative and 76% for Gram-positive). Hexane (HE) and chloroform extract (CE) were potent inhibitors of S. aureus biofilm and the microscopies further confirmed an important reduction in adherent cells. Polystyrene surfaces coated with these extracts showed a decrease in bacterial adhesion with the resulting inhibition of S. aureus biofilm formation and biofilm activity. Moreover, they also interfere with the coagulase and hemolysin activities of this bacterium. With respect to Gram-negative bacteria, these extracts showed anti-QS activity against Chromobacterium violaceum and inhibited biofilm formation, swarming motility, pyocyanin production, and elastase activity of Pseudomonas aeruginosa. Chemical analysis of HE and CE revealed that the fatty acids were the main compounds, particularly linolenic, oleic and hexadecanoic acids followed by the oxygenated monoterpenes. Non-toxic laurel extracts offer a natural alternative to control contamination and/or spoilage of food, as well as infectious diseases, by attacking the biofilm and the virulence of Gram-positive and Gram-negative bacteria.Fil: Molina, Rocío Daniela Inés. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; ArgentinaFil: Campos Silva, Rodrigo. Universidade Federal do Rio Grande do Sul; BrasilFil: Díaz, Myriam Anabel. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; ArgentinaFil: Macedo, Alexandre José. Universidade Federal do Rio Grande do Sul; BrasilFil: Blázquez, María Amparo. Universidad de Valencia; EspañaFil: Alberto, Maria Rosa. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Arena, Mario Eduardo. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaElsevier Science2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/143339Molina, Rocío Daniela Inés; Campos Silva, Rodrigo; Díaz, Myriam Anabel; Macedo, Alexandre José; Blázquez, María Amparo; et al.; Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens; Elsevier Science; LWT - Food Science and Technology; 134; 12-2020; 1-9; 1098990023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2020.109899info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643820308884info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:05Zoai:ri.conicet.gov.ar:11336/143339instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:05.595CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens
title Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens
spellingShingle Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens
Molina, Rocío Daniela Inés
BIOFILM
ENZYME INHIBITION
FATTY ACIDS
SWARMING
PYOCYANIN
title_short Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens
title_full Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens
title_fullStr Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens
title_full_unstemmed Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens
title_sort Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens
dc.creator.none.fl_str_mv Molina, Rocío Daniela Inés
Campos Silva, Rodrigo
Díaz, Myriam Anabel
Macedo, Alexandre José
Blázquez, María Amparo
Alberto, Maria Rosa
Arena, Mario Eduardo
author Molina, Rocío Daniela Inés
author_facet Molina, Rocío Daniela Inés
Campos Silva, Rodrigo
Díaz, Myriam Anabel
Macedo, Alexandre José
Blázquez, María Amparo
Alberto, Maria Rosa
Arena, Mario Eduardo
author_role author
author2 Campos Silva, Rodrigo
Díaz, Myriam Anabel
Macedo, Alexandre José
Blázquez, María Amparo
Alberto, Maria Rosa
Arena, Mario Eduardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOFILM
ENZYME INHIBITION
FATTY ACIDS
SWARMING
PYOCYANIN
topic BIOFILM
ENZYME INHIBITION
FATTY ACIDS
SWARMING
PYOCYANIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Antimicrobial, antibiofilm, anti-Quorum sensing (QS) and virulence factors inhibitory capacity of different polarity Laurus nobilis extracts against several pathogenic microorganisms were studied. Some extracts exhibited antibiotic effect against Staphylococcus aureus multidrug-resistant strains. However, all extracts (100 µg/mL) inhibited to some extent the biofilm of most bacteria tested (until 40% for Gram-negative and 76% for Gram-positive). Hexane (HE) and chloroform extract (CE) were potent inhibitors of S. aureus biofilm and the microscopies further confirmed an important reduction in adherent cells. Polystyrene surfaces coated with these extracts showed a decrease in bacterial adhesion with the resulting inhibition of S. aureus biofilm formation and biofilm activity. Moreover, they also interfere with the coagulase and hemolysin activities of this bacterium. With respect to Gram-negative bacteria, these extracts showed anti-QS activity against Chromobacterium violaceum and inhibited biofilm formation, swarming motility, pyocyanin production, and elastase activity of Pseudomonas aeruginosa. Chemical analysis of HE and CE revealed that the fatty acids were the main compounds, particularly linolenic, oleic and hexadecanoic acids followed by the oxygenated monoterpenes. Non-toxic laurel extracts offer a natural alternative to control contamination and/or spoilage of food, as well as infectious diseases, by attacking the biofilm and the virulence of Gram-positive and Gram-negative bacteria.
Fil: Molina, Rocío Daniela Inés. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina
Fil: Campos Silva, Rodrigo. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Díaz, Myriam Anabel. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina
Fil: Macedo, Alexandre José. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Blázquez, María Amparo. Universidad de Valencia; España
Fil: Alberto, Maria Rosa. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Arena, Mario Eduardo. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description Antimicrobial, antibiofilm, anti-Quorum sensing (QS) and virulence factors inhibitory capacity of different polarity Laurus nobilis extracts against several pathogenic microorganisms were studied. Some extracts exhibited antibiotic effect against Staphylococcus aureus multidrug-resistant strains. However, all extracts (100 µg/mL) inhibited to some extent the biofilm of most bacteria tested (until 40% for Gram-negative and 76% for Gram-positive). Hexane (HE) and chloroform extract (CE) were potent inhibitors of S. aureus biofilm and the microscopies further confirmed an important reduction in adherent cells. Polystyrene surfaces coated with these extracts showed a decrease in bacterial adhesion with the resulting inhibition of S. aureus biofilm formation and biofilm activity. Moreover, they also interfere with the coagulase and hemolysin activities of this bacterium. With respect to Gram-negative bacteria, these extracts showed anti-QS activity against Chromobacterium violaceum and inhibited biofilm formation, swarming motility, pyocyanin production, and elastase activity of Pseudomonas aeruginosa. Chemical analysis of HE and CE revealed that the fatty acids were the main compounds, particularly linolenic, oleic and hexadecanoic acids followed by the oxygenated monoterpenes. Non-toxic laurel extracts offer a natural alternative to control contamination and/or spoilage of food, as well as infectious diseases, by attacking the biofilm and the virulence of Gram-positive and Gram-negative bacteria.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/143339
Molina, Rocío Daniela Inés; Campos Silva, Rodrigo; Díaz, Myriam Anabel; Macedo, Alexandre José; Blázquez, María Amparo; et al.; Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens; Elsevier Science; LWT - Food Science and Technology; 134; 12-2020; 1-9; 109899
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/143339
identifier_str_mv Molina, Rocío Daniela Inés; Campos Silva, Rodrigo; Díaz, Myriam Anabel; Macedo, Alexandre José; Blázquez, María Amparo; et al.; Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens; Elsevier Science; LWT - Food Science and Technology; 134; 12-2020; 1-9; 109899
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2020.109899
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0023643820308884
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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