Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins
- Autores
- Mechaly, Ariel Edgardo; Bellomio, Augusto; Gil Cartón, David; Morante, Koldo; Valle, Mikel; Gonzalez Mañas, Juan Manuel; Guérin, Diego M. A.
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Pore-forming toxins (PFTs) are proteins that are secreted as soluble molecules and are inserted into membranes to form oligomeric transmembrane pores. In this paper, we report the crystal structure of Fragaceatoxin C (FraC), a PFT isolated from the sea anemone Actinia fragacea, at 1.8 resolution. It consists of a crown-shaped nonamer with an external diameter of about 11.0 nm and an internal diameter of approximately 5.0 nm. Cryoelectron microscopy studies of FraC in lipid bilayers reveal the pore structure that traverses the membrane. The shape and dimensions of the crystallographic oligomer are fully consistent with the membrane pore. The FraC structure provides insight into the interactions governing the assembly process and suggests the structural changes that allow for membrane insertion. We propose a nonameric pore model that spans the membrane by forming a lipid-free α-helical bundle pore.
Fil: Mechaly, Ariel Edgardo. Universidad del País Vasco; España
Fil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad del País Vasco; España
Fil: Gil Cartón, David. Cooperative Center for Research in Biosciences; España
Fil: Morante, Koldo. Universidad del País Vasco; España
Fil: Valle, Mikel. Universidad del País Vasco; España
Fil: Gonzalez Mañas, Juan Manuel. Universidad del País Vasco; España
Fil: Guérin, Diego M. A.. Universidad del País Vasco; España - Materia
-
Membrane Pore
Toxins
Actinoporins
Pore-Forming Proteins - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/62824
Ver los metadatos del registro completo
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Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxinsMechaly, Ariel EdgardoBellomio, AugustoGil Cartón, DavidMorante, KoldoValle, MikelGonzalez Mañas, Juan ManuelGuérin, Diego M. A.Membrane PoreToxinsActinoporinsPore-Forming Proteinshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pore-forming toxins (PFTs) are proteins that are secreted as soluble molecules and are inserted into membranes to form oligomeric transmembrane pores. In this paper, we report the crystal structure of Fragaceatoxin C (FraC), a PFT isolated from the sea anemone Actinia fragacea, at 1.8 resolution. It consists of a crown-shaped nonamer with an external diameter of about 11.0 nm and an internal diameter of approximately 5.0 nm. Cryoelectron microscopy studies of FraC in lipid bilayers reveal the pore structure that traverses the membrane. The shape and dimensions of the crystallographic oligomer are fully consistent with the membrane pore. The FraC structure provides insight into the interactions governing the assembly process and suggests the structural changes that allow for membrane insertion. We propose a nonameric pore model that spans the membrane by forming a lipid-free α-helical bundle pore.Fil: Mechaly, Ariel Edgardo. Universidad del País Vasco; EspañaFil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad del País Vasco; EspañaFil: Gil Cartón, David. Cooperative Center for Research in Biosciences; EspañaFil: Morante, Koldo. Universidad del País Vasco; EspañaFil: Valle, Mikel. Universidad del País Vasco; EspañaFil: Gonzalez Mañas, Juan Manuel. Universidad del País Vasco; EspañaFil: Guérin, Diego M. A.. Universidad del País Vasco; EspañaElsevier2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62824Mechaly, Ariel Edgardo; Bellomio, Augusto; Gil Cartón, David; Morante, Koldo; Valle, Mikel; et al.; Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins; Elsevier; Structure With Folding & Design.; 19; 2; 2-2011; 181-1910969-2126CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.str.2010.11.013info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0969212610004375info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:29Zoai:ri.conicet.gov.ar:11336/62824instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:29.916CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins |
title |
Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins |
spellingShingle |
Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins Mechaly, Ariel Edgardo Membrane Pore Toxins Actinoporins Pore-Forming Proteins |
title_short |
Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins |
title_full |
Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins |
title_fullStr |
Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins |
title_full_unstemmed |
Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins |
title_sort |
Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins |
dc.creator.none.fl_str_mv |
Mechaly, Ariel Edgardo Bellomio, Augusto Gil Cartón, David Morante, Koldo Valle, Mikel Gonzalez Mañas, Juan Manuel Guérin, Diego M. A. |
author |
Mechaly, Ariel Edgardo |
author_facet |
Mechaly, Ariel Edgardo Bellomio, Augusto Gil Cartón, David Morante, Koldo Valle, Mikel Gonzalez Mañas, Juan Manuel Guérin, Diego M. A. |
author_role |
author |
author2 |
Bellomio, Augusto Gil Cartón, David Morante, Koldo Valle, Mikel Gonzalez Mañas, Juan Manuel Guérin, Diego M. A. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Membrane Pore Toxins Actinoporins Pore-Forming Proteins |
topic |
Membrane Pore Toxins Actinoporins Pore-Forming Proteins |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Pore-forming toxins (PFTs) are proteins that are secreted as soluble molecules and are inserted into membranes to form oligomeric transmembrane pores. In this paper, we report the crystal structure of Fragaceatoxin C (FraC), a PFT isolated from the sea anemone Actinia fragacea, at 1.8 resolution. It consists of a crown-shaped nonamer with an external diameter of about 11.0 nm and an internal diameter of approximately 5.0 nm. Cryoelectron microscopy studies of FraC in lipid bilayers reveal the pore structure that traverses the membrane. The shape and dimensions of the crystallographic oligomer are fully consistent with the membrane pore. The FraC structure provides insight into the interactions governing the assembly process and suggests the structural changes that allow for membrane insertion. We propose a nonameric pore model that spans the membrane by forming a lipid-free α-helical bundle pore. Fil: Mechaly, Ariel Edgardo. Universidad del País Vasco; España Fil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad del País Vasco; España Fil: Gil Cartón, David. Cooperative Center for Research in Biosciences; España Fil: Morante, Koldo. Universidad del País Vasco; España Fil: Valle, Mikel. Universidad del País Vasco; España Fil: Gonzalez Mañas, Juan Manuel. Universidad del País Vasco; España Fil: Guérin, Diego M. A.. Universidad del País Vasco; España |
description |
Pore-forming toxins (PFTs) are proteins that are secreted as soluble molecules and are inserted into membranes to form oligomeric transmembrane pores. In this paper, we report the crystal structure of Fragaceatoxin C (FraC), a PFT isolated from the sea anemone Actinia fragacea, at 1.8 resolution. It consists of a crown-shaped nonamer with an external diameter of about 11.0 nm and an internal diameter of approximately 5.0 nm. Cryoelectron microscopy studies of FraC in lipid bilayers reveal the pore structure that traverses the membrane. The shape and dimensions of the crystallographic oligomer are fully consistent with the membrane pore. The FraC structure provides insight into the interactions governing the assembly process and suggests the structural changes that allow for membrane insertion. We propose a nonameric pore model that spans the membrane by forming a lipid-free α-helical bundle pore. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/62824 Mechaly, Ariel Edgardo; Bellomio, Augusto; Gil Cartón, David; Morante, Koldo; Valle, Mikel; et al.; Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins; Elsevier; Structure With Folding & Design.; 19; 2; 2-2011; 181-191 0969-2126 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/62824 |
identifier_str_mv |
Mechaly, Ariel Edgardo; Bellomio, Augusto; Gil Cartón, David; Morante, Koldo; Valle, Mikel; et al.; Structural insights into the oligomerization and architecture of eukaryotic membrane pore-forming toxins; Elsevier; Structure With Folding & Design.; 19; 2; 2-2011; 181-191 0969-2126 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.str.2010.11.013 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0969212610004375 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |