The degree of enhancer or promoter activity is reflected by the levels and directionality of eRNA transcription

• Autores: Mikhaylichenko, Olga; Bondarenko, Vladyslav; Harnett, Dermot; Schor, Ignacio Esteban; Males, Matilda; Viales, Rebecca R.; Furlong, Eileen E. M.
• Año de publicación: 2018
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: DEVELOPMENTAL ENHANCERS; EMBRYONIC DEVELOPMENT]; ERNA; NCRNA; PROMOTERS; SPATIO; TEMPORAL EXPRESSION
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Gene expression is regulated by promoters, which initiate transcription, and enhancers, which control their temporal and spatial activity. However, the discovery that mammalian enhancers also initiate transcription questions the inherent differences between enhancers and promoters. Here, we investigate the transcriptional properties of enhancers during Drosophila embryogenesis using characterized developmental enhancers. We show that while the timing of enhancer transcription is generally correlated with enhancer activity, the levels and directionality of transcription are highly varied among active enhancers. To assess how this impacts function, we developed a dual transgenic assay to simultaneously measure enhancer and promoter activities from a single element in the same embryo. Extensive transgenic analysis revealed a relationship between the direction of endogenous transcription and the ability to function as an enhancer or promoter in vivo, although enhancer RNA (eRNA) production and activity are not always strictly coupled. Some enhancers (mainly bidirectional) can act as weak promoters, producing overlapping spatio–temporal expression. Conversely, bidirectional promoters often act as strong enhancers, while unidirectional promoters generally cannot. The balance between enhancer and promoter activity is generally reflected in the levels and directionality of eRNA transcription and is likely an inherent sequence property of the elements themselves.
Fil: Mikhaylichenko, Olga. European Molecular Biology Laboratory; Alemania
Fil: Bondarenko, Vladyslav. European Molecular Biology Laboratory; Alemania
Fil: Harnett, Dermot. European Molecular Biology Laboratory; Alemania
Fil: Schor, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Males, Matilda. European Molecular Biology Laboratory; Alemania
Fil: Viales, Rebecca R.. European Molecular Biology Laboratory; Alemania
Fil: Furlong, Eileen E. M.. European Molecular Biology Laboratory; Alemania