Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy

Autores
Stoyanoff, Tania Romina; Todaro, Juan Santiago; Aguirre, María Victoria; Brandan, Nora Cristina
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adriamycin (ADR)-induced nephropathy in rodents is an experimental model commonly used for studies of chronic human renal diseases. The molecular associations involved in renal apoptosis linked to hypoxia and cell stress response in this model are not completely known. The aim of this study was to determine the associations among the expression patterns of the Inducible Nitric Oxide Synthase (iNOS), the heat shock protein 60 (Hsp60) and the Hypoxia Inducible Factor-1 Alpha (HIF-1α) linked to apoptosis in renal cortex in the nephrotic syndrome progression induced by ADR administration. Male BALB/c mice were treated with a single dose of ADR (11 mg kg-1; i.v.). Tubulointerstitial nephrosis was monitored by histopathological assessment and by biochemical determinations on 7, 15 and 30 days following drug exposure. These results were evaluated in conjunction with renal expression of iNOS, Hsp60 and HIF-1α. Cortical apoptosis was examined by TUNEL assay. The increment of renal apoptotic cells in tubulointerstitial areas was accompanied by the decrease in Bcl-xL/Bax ratio and the enhancement of the active caspase-3 and Hsp60 expressions from day 7 onwards. iNOS and HIF-1α increased concomitant with the renal apoptosis and the tubule interstitial injury. Taking the previous information into account, data indicate that the over expression of renal HIF-1α, iNOS and Hsp60 are concurrent with the apoptotic events triggered by ADR. These results contribute to additional knowledge of the molecular complex events involved in the context of ADR-induced nephropathy progression.
Fil: Stoyanoff, Tania Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; Argentina
Fil: Todaro, Juan Santiago. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; Argentina
Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; Argentina
Fil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; Argentina
Materia
APOPTOSIS
ADRYAMICIN INDUCED-NEPHROPATHY
INDUCIBLE NITRIC OXIDE SYNTHASE
RENAL HYPOXIA
HSP60
HIF-1α
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54337
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54337
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathyStoyanoff, Tania RominaTodaro, Juan SantiagoAguirre, María VictoriaBrandan, Nora CristinaAPOPTOSISADRYAMICIN INDUCED-NEPHROPATHYINDUCIBLE NITRIC OXIDE SYNTHASERENAL HYPOXIAHSP60HIF-1αhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Adriamycin (ADR)-induced nephropathy in rodents is an experimental model commonly used for studies of chronic human renal diseases. The molecular associations involved in renal apoptosis linked to hypoxia and cell stress response in this model are not completely known. The aim of this study was to determine the associations among the expression patterns of the Inducible Nitric Oxide Synthase (iNOS), the heat shock protein 60 (Hsp60) and the Hypoxia Inducible Factor-1 Alpha (HIF-1α) linked to apoptosis in renal cortex in the nephrotic syndrome progression induced by ADR administration. Male BALB/c mice were treated with a single dose of ADR (11 mg kg-1; i.v.). Tubulointerstitial nephrosis was monitored by histopathological assessment and by biochemical determinations on 7, 15 and 30 days following drug exposure. These results were evaluated in conjunction with renal expression of iNOS, Hsp60 and HIF-1α. Cortical apoptosis was examined by TUNEL assay. The increment of renal apoptotic cells in tubulointerstitial areas was accompanied by the decrease in Bcl-xL/Bax ratio and the enhancement of the active caspase-3 and Hsp60 expressions from day 7 onwards. iNOS and HIF-1α increased concomitant with the renal apoptosis and the tubule interstitial injury. Taking the previous information into account, data indicate that the over expression of renal HIF-1α, iNOS and Hsp60 are concurrent with the apoptotic events triggered by ADR. These results contribute to additional knowledge of the molecular complex events involved in the context of ADR-induced nephropathy progression.Fil: Stoyanoff, Tania Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; ArgentinaFil: Todaro, Juan Santiago. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; ArgentinaFil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; ArgentinaFil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; ArgentinaAcademic Journals Inc2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54337Stoyanoff, Tania Romina; Todaro, Juan Santiago; Aguirre, María Victoria; Brandan, Nora Cristina; Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy; Academic Journals Inc; Journal of Pharmacology and Toxicology; 7; 8; 12-2012; 344-3581816-496X2152-100XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://scialert.net/abstract/?doi=jpt.2012.344.358info:eu-repo/semantics/altIdentifier/doi/10.3923/jpt.2012.344.358info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:07:26Zoai:ri.conicet.gov.ar:11336/54337instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:07:27.127CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy
title Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy
spellingShingle Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy
Stoyanoff, Tania Romina
APOPTOSIS
ADRYAMICIN INDUCED-NEPHROPATHY
INDUCIBLE NITRIC OXIDE SYNTHASE
RENAL HYPOXIA
HSP60
HIF-1α
title_short Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy
title_full Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy
title_fullStr Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy
title_full_unstemmed Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy
title_sort Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy
dc.creator.none.fl_str_mv Stoyanoff, Tania Romina
Todaro, Juan Santiago
Aguirre, María Victoria
Brandan, Nora Cristina
author Stoyanoff, Tania Romina
author_facet Stoyanoff, Tania Romina
Todaro, Juan Santiago
Aguirre, María Victoria
Brandan, Nora Cristina
author_role author
author2 Todaro, Juan Santiago
Aguirre, María Victoria
Brandan, Nora Cristina
author2_role author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
ADRYAMICIN INDUCED-NEPHROPATHY
INDUCIBLE NITRIC OXIDE SYNTHASE
RENAL HYPOXIA
HSP60
HIF-1α
topic APOPTOSIS
ADRYAMICIN INDUCED-NEPHROPATHY
INDUCIBLE NITRIC OXIDE SYNTHASE
RENAL HYPOXIA
HSP60
HIF-1α
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Adriamycin (ADR)-induced nephropathy in rodents is an experimental model commonly used for studies of chronic human renal diseases. The molecular associations involved in renal apoptosis linked to hypoxia and cell stress response in this model are not completely known. The aim of this study was to determine the associations among the expression patterns of the Inducible Nitric Oxide Synthase (iNOS), the heat shock protein 60 (Hsp60) and the Hypoxia Inducible Factor-1 Alpha (HIF-1α) linked to apoptosis in renal cortex in the nephrotic syndrome progression induced by ADR administration. Male BALB/c mice were treated with a single dose of ADR (11 mg kg-1; i.v.). Tubulointerstitial nephrosis was monitored by histopathological assessment and by biochemical determinations on 7, 15 and 30 days following drug exposure. These results were evaluated in conjunction with renal expression of iNOS, Hsp60 and HIF-1α. Cortical apoptosis was examined by TUNEL assay. The increment of renal apoptotic cells in tubulointerstitial areas was accompanied by the decrease in Bcl-xL/Bax ratio and the enhancement of the active caspase-3 and Hsp60 expressions from day 7 onwards. iNOS and HIF-1α increased concomitant with the renal apoptosis and the tubule interstitial injury. Taking the previous information into account, data indicate that the over expression of renal HIF-1α, iNOS and Hsp60 are concurrent with the apoptotic events triggered by ADR. These results contribute to additional knowledge of the molecular complex events involved in the context of ADR-induced nephropathy progression.
Fil: Stoyanoff, Tania Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; Argentina
Fil: Todaro, Juan Santiago. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; Argentina
Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; Argentina
Fil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Medicina. Cátedra de Bioquímica; Argentina
description Adriamycin (ADR)-induced nephropathy in rodents is an experimental model commonly used for studies of chronic human renal diseases. The molecular associations involved in renal apoptosis linked to hypoxia and cell stress response in this model are not completely known. The aim of this study was to determine the associations among the expression patterns of the Inducible Nitric Oxide Synthase (iNOS), the heat shock protein 60 (Hsp60) and the Hypoxia Inducible Factor-1 Alpha (HIF-1α) linked to apoptosis in renal cortex in the nephrotic syndrome progression induced by ADR administration. Male BALB/c mice were treated with a single dose of ADR (11 mg kg-1; i.v.). Tubulointerstitial nephrosis was monitored by histopathological assessment and by biochemical determinations on 7, 15 and 30 days following drug exposure. These results were evaluated in conjunction with renal expression of iNOS, Hsp60 and HIF-1α. Cortical apoptosis was examined by TUNEL assay. The increment of renal apoptotic cells in tubulointerstitial areas was accompanied by the decrease in Bcl-xL/Bax ratio and the enhancement of the active caspase-3 and Hsp60 expressions from day 7 onwards. iNOS and HIF-1α increased concomitant with the renal apoptosis and the tubule interstitial injury. Taking the previous information into account, data indicate that the over expression of renal HIF-1α, iNOS and Hsp60 are concurrent with the apoptotic events triggered by ADR. These results contribute to additional knowledge of the molecular complex events involved in the context of ADR-induced nephropathy progression.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54337
Stoyanoff, Tania Romina; Todaro, Juan Santiago; Aguirre, María Victoria; Brandan, Nora Cristina; Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy; Academic Journals Inc; Journal of Pharmacology and Toxicology; 7; 8; 12-2012; 344-358
1816-496X
2152-100X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54337
identifier_str_mv Stoyanoff, Tania Romina; Todaro, Juan Santiago; Aguirre, María Victoria; Brandan, Nora Cristina; Cell stress, hypoxic response and apoptosis in murine adriamycin-induced nephropathy; Academic Journals Inc; Journal of Pharmacology and Toxicology; 7; 8; 12-2012; 344-358
1816-496X
2152-100X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://scialert.net/abstract/?doi=jpt.2012.344.358
info:eu-repo/semantics/altIdentifier/doi/10.3923/jpt.2012.344.358
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Journals Inc
publisher.none.fl_str_mv Academic Journals Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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