Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination

Autores
Echeverria, Pablo Christian; Dran, Graciela Isabel; Pereda, Graciela; Rico, Ana I.; Requena, José M.; Alonso, Carlos; Guarnera, Eduardo; Ángel, Sergio Oscar
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The properties of Leishmania infantum hsp83 (LiHsp83) to elicit an immune response against a fused reporter antigen, maltose binding protein (MBP), was studied. CF1 mice were immunized with different purified recombinant proteins: MBP, LiHsp83 and MBP fused to LiHsp83 (MBP-LiHsp83). Serum samples were obtained at days 0, 21, 28, 60, 90, 120 and 150 post-immunization. MBP-LiHsp83 fusion protein elicited a strong humoral response against MBP, higher than that one obtained in mice immunized with MBP alone or MBP mixed with LiHsp83, showing the secretion of both anti-MBP IgG2a and IgG1 isotypes (IgG2a/IgG1 ratio: 2:1). This response was specific for recombinant proteins and was maintained for at least 150 days, whereas the reactivity in mice immunized with MBP alone dissapeared at day 90. After in vitro stimulation with MBP, spleen cells from MBP-LiHsp83 immunized mice showed higher proliferation indices and produced higher secretion of IFN-γ than spleen cells from either control or MBP-immunized mice. In all groups of mice IL-4 was undetectable. Thus we consider that LiHsp83 may be a promising candidate to be used as carrier of fused antigens for adjuvant-free vaccination. © 2001 Elsevier Science B.V.
Fil: Echeverria, Pablo Christian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Dran, Graciela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Pereda, Graciela. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Rico, Ana I.. Centro de Biologia Molecular Severo Ochoa; España
Fil: Requena, José M.. Centro de Biologia Molecular Severo Ochoa; España
Fil: Alonso, Carlos. Centro de Biologia Molecular Severo Ochoa; España
Fil: Guarnera, Eduardo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Ángel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Materia
Adjuvant
Carrier
Heat Shock Protein
Hsp83
Vaccine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54828
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54828
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccinationEcheverria, Pablo ChristianDran, Graciela IsabelPereda, GracielaRico, Ana I.Requena, José M.Alonso, CarlosGuarnera, EduardoÁngel, Sergio OscarAdjuvantCarrierHeat Shock ProteinHsp83Vaccinehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The properties of Leishmania infantum hsp83 (LiHsp83) to elicit an immune response against a fused reporter antigen, maltose binding protein (MBP), was studied. CF1 mice were immunized with different purified recombinant proteins: MBP, LiHsp83 and MBP fused to LiHsp83 (MBP-LiHsp83). Serum samples were obtained at days 0, 21, 28, 60, 90, 120 and 150 post-immunization. MBP-LiHsp83 fusion protein elicited a strong humoral response against MBP, higher than that one obtained in mice immunized with MBP alone or MBP mixed with LiHsp83, showing the secretion of both anti-MBP IgG2a and IgG1 isotypes (IgG2a/IgG1 ratio: 2:1). This response was specific for recombinant proteins and was maintained for at least 150 days, whereas the reactivity in mice immunized with MBP alone dissapeared at day 90. After in vitro stimulation with MBP, spleen cells from MBP-LiHsp83 immunized mice showed higher proliferation indices and produced higher secretion of IFN-γ than spleen cells from either control or MBP-immunized mice. In all groups of mice IL-4 was undetectable. Thus we consider that LiHsp83 may be a promising candidate to be used as carrier of fused antigens for adjuvant-free vaccination. © 2001 Elsevier Science B.V.Fil: Echeverria, Pablo Christian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Dran, Graciela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Pereda, Graciela. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Rico, Ana I.. Centro de Biologia Molecular Severo Ochoa; EspañaFil: Requena, José M.. Centro de Biologia Molecular Severo Ochoa; EspañaFil: Alonso, Carlos. Centro de Biologia Molecular Severo Ochoa; EspañaFil: Guarnera, Eduardo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Ángel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaElsevier Science2001-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54828Echeverria, Pablo Christian; Dran, Graciela Isabel; Pereda, Graciela; Rico, Ana I.; Requena, José M.; et al.; Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination; Elsevier Science; Immunology Letters; 76; 2; 3-2001; 107-1100165-2478CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0165-2478(01)00179-1info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165247801001791info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:09Zoai:ri.conicet.gov.ar:11336/54828instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:09.752CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination
title Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination
spellingShingle Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination
Echeverria, Pablo Christian
Adjuvant
Carrier
Heat Shock Protein
Hsp83
Vaccine
title_short Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination
title_full Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination
title_fullStr Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination
title_full_unstemmed Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination
title_sort Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination
dc.creator.none.fl_str_mv Echeverria, Pablo Christian
Dran, Graciela Isabel
Pereda, Graciela
Rico, Ana I.
Requena, José M.
Alonso, Carlos
Guarnera, Eduardo
Ángel, Sergio Oscar
author Echeverria, Pablo Christian
author_facet Echeverria, Pablo Christian
Dran, Graciela Isabel
Pereda, Graciela
Rico, Ana I.
Requena, José M.
Alonso, Carlos
Guarnera, Eduardo
Ángel, Sergio Oscar
author_role author
author2 Dran, Graciela Isabel
Pereda, Graciela
Rico, Ana I.
Requena, José M.
Alonso, Carlos
Guarnera, Eduardo
Ángel, Sergio Oscar
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adjuvant
Carrier
Heat Shock Protein
Hsp83
Vaccine
topic Adjuvant
Carrier
Heat Shock Protein
Hsp83
Vaccine
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The properties of Leishmania infantum hsp83 (LiHsp83) to elicit an immune response against a fused reporter antigen, maltose binding protein (MBP), was studied. CF1 mice were immunized with different purified recombinant proteins: MBP, LiHsp83 and MBP fused to LiHsp83 (MBP-LiHsp83). Serum samples were obtained at days 0, 21, 28, 60, 90, 120 and 150 post-immunization. MBP-LiHsp83 fusion protein elicited a strong humoral response against MBP, higher than that one obtained in mice immunized with MBP alone or MBP mixed with LiHsp83, showing the secretion of both anti-MBP IgG2a and IgG1 isotypes (IgG2a/IgG1 ratio: 2:1). This response was specific for recombinant proteins and was maintained for at least 150 days, whereas the reactivity in mice immunized with MBP alone dissapeared at day 90. After in vitro stimulation with MBP, spleen cells from MBP-LiHsp83 immunized mice showed higher proliferation indices and produced higher secretion of IFN-γ than spleen cells from either control or MBP-immunized mice. In all groups of mice IL-4 was undetectable. Thus we consider that LiHsp83 may be a promising candidate to be used as carrier of fused antigens for adjuvant-free vaccination. © 2001 Elsevier Science B.V.
Fil: Echeverria, Pablo Christian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Dran, Graciela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Pereda, Graciela. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Rico, Ana I.. Centro de Biologia Molecular Severo Ochoa; España
Fil: Requena, José M.. Centro de Biologia Molecular Severo Ochoa; España
Fil: Alonso, Carlos. Centro de Biologia Molecular Severo Ochoa; España
Fil: Guarnera, Eduardo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Ángel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
description The properties of Leishmania infantum hsp83 (LiHsp83) to elicit an immune response against a fused reporter antigen, maltose binding protein (MBP), was studied. CF1 mice were immunized with different purified recombinant proteins: MBP, LiHsp83 and MBP fused to LiHsp83 (MBP-LiHsp83). Serum samples were obtained at days 0, 21, 28, 60, 90, 120 and 150 post-immunization. MBP-LiHsp83 fusion protein elicited a strong humoral response against MBP, higher than that one obtained in mice immunized with MBP alone or MBP mixed with LiHsp83, showing the secretion of both anti-MBP IgG2a and IgG1 isotypes (IgG2a/IgG1 ratio: 2:1). This response was specific for recombinant proteins and was maintained for at least 150 days, whereas the reactivity in mice immunized with MBP alone dissapeared at day 90. After in vitro stimulation with MBP, spleen cells from MBP-LiHsp83 immunized mice showed higher proliferation indices and produced higher secretion of IFN-γ than spleen cells from either control or MBP-immunized mice. In all groups of mice IL-4 was undetectable. Thus we consider that LiHsp83 may be a promising candidate to be used as carrier of fused antigens for adjuvant-free vaccination. © 2001 Elsevier Science B.V.
publishDate 2001
dc.date.none.fl_str_mv 2001-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54828
Echeverria, Pablo Christian; Dran, Graciela Isabel; Pereda, Graciela; Rico, Ana I.; Requena, José M.; et al.; Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination; Elsevier Science; Immunology Letters; 76; 2; 3-2001; 107-110
0165-2478
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54828
identifier_str_mv Echeverria, Pablo Christian; Dran, Graciela Isabel; Pereda, Graciela; Rico, Ana I.; Requena, José M.; et al.; Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination; Elsevier Science; Immunology Letters; 76; 2; 3-2001; 107-110
0165-2478
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0165-2478(01)00179-1
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0165247801001791
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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