Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana

Autores
Zawoznik, Myriam Sara; Groppa, María Daniela; Tomaro, Maria Lujan; Benavides, Maria Patricia
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To better understand the role of endogenous salicylic acid (SA) in plants exposed to abiotic stresses known to generate oxidative damage, the response to cadmium treatment of a wild Arabidopsis thaliana ecotype and a SA-deficient transgenic line was investigated. After 5 days of Cd treatment, chlorophyll content was significantly reduced and TBARS significantly increased in wild type seedlings but not in the SA-deficient line. Leaves of wild type plants exposed to the metal showed accumulation of H2O2 and increased oxidized glutathione content, resulting in decreased GSH/GSSG ratios. After Cd treatment, transgenic plants displayed a significantly decreased SOD activity (about 50% of control) which may have contributed to prevent H2O2 increase. The activity of several H2O2-detoxyfing enzymes diminished in wild type seedlings exposed to the metal (27–35% decrease). In SA-deficient plants ascorbate peroxidase and glutathione peroxidase activities slightly decreased, but guaiacol peroxidase and catalase activities significantly increased. CAT1 isoform was found to be particularly abundant in wild type Arabidopsis plants exposed to Cd, but scarcely expressed and almost unaffected by the metal addition in the transgenic line. Even when protective effects of SA have been extensively reported for several plant species, our results suggest that endogenous SA may function in A. thaliana as a signaling molecule necessary to generate, to sustain or to amplify Cd-induced oxidative stress.
Fil: Zawoznik, Myriam Sara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina
Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina
Fil: Tomaro, Maria Lujan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Materia
OXIDATIVE STRESS
SALICYLIC ACID
CATALASE
ARABIDOPSIS THALIANA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241937
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/241937
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thalianaZawoznik, Myriam SaraGroppa, María DanielaTomaro, Maria LujanBenavides, Maria PatriciaOXIDATIVE STRESSSALICYLIC ACIDCATALASEARABIDOPSIS THALIANAhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1To better understand the role of endogenous salicylic acid (SA) in plants exposed to abiotic stresses known to generate oxidative damage, the response to cadmium treatment of a wild Arabidopsis thaliana ecotype and a SA-deficient transgenic line was investigated. After 5 days of Cd treatment, chlorophyll content was significantly reduced and TBARS significantly increased in wild type seedlings but not in the SA-deficient line. Leaves of wild type plants exposed to the metal showed accumulation of H2O2 and increased oxidized glutathione content, resulting in decreased GSH/GSSG ratios. After Cd treatment, transgenic plants displayed a significantly decreased SOD activity (about 50% of control) which may have contributed to prevent H2O2 increase. The activity of several H2O2-detoxyfing enzymes diminished in wild type seedlings exposed to the metal (27–35% decrease). In SA-deficient plants ascorbate peroxidase and glutathione peroxidase activities slightly decreased, but guaiacol peroxidase and catalase activities significantly increased. CAT1 isoform was found to be particularly abundant in wild type Arabidopsis plants exposed to Cd, but scarcely expressed and almost unaffected by the metal addition in the transgenic line. Even when protective effects of SA have been extensively reported for several plant species, our results suggest that endogenous SA may function in A. thaliana as a signaling molecule necessary to generate, to sustain or to amplify Cd-induced oxidative stress.Fil: Zawoznik, Myriam Sara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; ArgentinaFil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; ArgentinaFil: Tomaro, Maria Lujan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaElsevier Ireland2007-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241937Zawoznik, Myriam Sara; Groppa, María Daniela; Tomaro, Maria Lujan; Benavides, Maria Patricia; Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana; Elsevier Ireland; Plant Science; 173; 2; 8-2007; 190-1970168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945207001537info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2007.05.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:17Zoai:ri.conicet.gov.ar:11336/241937instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:18.263CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana
title Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana
spellingShingle Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana
Zawoznik, Myriam Sara
OXIDATIVE STRESS
SALICYLIC ACID
CATALASE
ARABIDOPSIS THALIANA
title_short Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana
title_full Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana
title_fullStr Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana
title_full_unstemmed Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana
title_sort Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana
dc.creator.none.fl_str_mv Zawoznik, Myriam Sara
Groppa, María Daniela
Tomaro, Maria Lujan
Benavides, Maria Patricia
author Zawoznik, Myriam Sara
author_facet Zawoznik, Myriam Sara
Groppa, María Daniela
Tomaro, Maria Lujan
Benavides, Maria Patricia
author_role author
author2 Groppa, María Daniela
Tomaro, Maria Lujan
Benavides, Maria Patricia
author2_role author
author
author
dc.subject.none.fl_str_mv OXIDATIVE STRESS
SALICYLIC ACID
CATALASE
ARABIDOPSIS THALIANA
topic OXIDATIVE STRESS
SALICYLIC ACID
CATALASE
ARABIDOPSIS THALIANA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To better understand the role of endogenous salicylic acid (SA) in plants exposed to abiotic stresses known to generate oxidative damage, the response to cadmium treatment of a wild Arabidopsis thaliana ecotype and a SA-deficient transgenic line was investigated. After 5 days of Cd treatment, chlorophyll content was significantly reduced and TBARS significantly increased in wild type seedlings but not in the SA-deficient line. Leaves of wild type plants exposed to the metal showed accumulation of H2O2 and increased oxidized glutathione content, resulting in decreased GSH/GSSG ratios. After Cd treatment, transgenic plants displayed a significantly decreased SOD activity (about 50% of control) which may have contributed to prevent H2O2 increase. The activity of several H2O2-detoxyfing enzymes diminished in wild type seedlings exposed to the metal (27–35% decrease). In SA-deficient plants ascorbate peroxidase and glutathione peroxidase activities slightly decreased, but guaiacol peroxidase and catalase activities significantly increased. CAT1 isoform was found to be particularly abundant in wild type Arabidopsis plants exposed to Cd, but scarcely expressed and almost unaffected by the metal addition in the transgenic line. Even when protective effects of SA have been extensively reported for several plant species, our results suggest that endogenous SA may function in A. thaliana as a signaling molecule necessary to generate, to sustain or to amplify Cd-induced oxidative stress.
Fil: Zawoznik, Myriam Sara. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina
Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina
Fil: Tomaro, Maria Lujan. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Benavides, Maria Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica. Cátedra de Química Biológica Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
description To better understand the role of endogenous salicylic acid (SA) in plants exposed to abiotic stresses known to generate oxidative damage, the response to cadmium treatment of a wild Arabidopsis thaliana ecotype and a SA-deficient transgenic line was investigated. After 5 days of Cd treatment, chlorophyll content was significantly reduced and TBARS significantly increased in wild type seedlings but not in the SA-deficient line. Leaves of wild type plants exposed to the metal showed accumulation of H2O2 and increased oxidized glutathione content, resulting in decreased GSH/GSSG ratios. After Cd treatment, transgenic plants displayed a significantly decreased SOD activity (about 50% of control) which may have contributed to prevent H2O2 increase. The activity of several H2O2-detoxyfing enzymes diminished in wild type seedlings exposed to the metal (27–35% decrease). In SA-deficient plants ascorbate peroxidase and glutathione peroxidase activities slightly decreased, but guaiacol peroxidase and catalase activities significantly increased. CAT1 isoform was found to be particularly abundant in wild type Arabidopsis plants exposed to Cd, but scarcely expressed and almost unaffected by the metal addition in the transgenic line. Even when protective effects of SA have been extensively reported for several plant species, our results suggest that endogenous SA may function in A. thaliana as a signaling molecule necessary to generate, to sustain or to amplify Cd-induced oxidative stress.
publishDate 2007
dc.date.none.fl_str_mv 2007-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241937
Zawoznik, Myriam Sara; Groppa, María Daniela; Tomaro, Maria Lujan; Benavides, Maria Patricia; Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana; Elsevier Ireland; Plant Science; 173; 2; 8-2007; 190-197
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241937
identifier_str_mv Zawoznik, Myriam Sara; Groppa, María Daniela; Tomaro, Maria Lujan; Benavides, Maria Patricia; Endogenous salicylic acid potentiates cadmium-induced oxidative stress in Arabidopsis thaliana; Elsevier Ireland; Plant Science; 173; 2; 8-2007; 190-197
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945207001537
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2007.05.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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