Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors

Autores
Kulkarni, Madhusudan B.; Reed, Matthew S.; Cao, Xu; García, Héctor Alfredo; Ochoa, Marien I.; Jiang, Shudong; Hasan, Tayyaba; Doyley, Marvin M.; Pogue, Brian W.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Significance: Fluorescence sensing within tissue is an effective tool for tissue characterization; however, the modality and geometry of the image acquisition can alter the observed signal.Aim: We introduce a novel optical fiber-based system capable of measuring two fluorescent contrast agents through 2 cm of tissue with simple passive electronic switching between the excitation light, simultaneously acquiring fluorescence and excitation data. The goal was to quantify indocyanine green (ICG) and protoporphyrin IX (PpIX) within tissue, and the sampling method was compared with wide-field surface imaging to contrast the value of deep sensing versus surface imaging.Approach: This was achieved by choosing filters for specific wavelengths that were mutually exclusive between ICG and PpIX and coupling these filters to two separate detectors, which allows for direct swapping of the excitation and emission channels by switching the on-time of each excitation laser between 780- and 633-nm wavelengths.Results: This system was compared with two non-contact surface imaging systems for both ICG and PpIX, which revealed that the fluorescence depth sensing system was superior in its ability to resolve kinetics differences in deeper tissues that would normally be dominated by strong signals from skin and other surface tissues. Specifically, the system was tested using pancreatic adenocarcinoma tumors injected into murine models, which were imaged at several time points throughout tumor growth to its ∼6-mm diameter. This demonstrated the system’s capability to track longitudinal changes in ICG and PpIX kinetics that result from tumor growth and development, with larger tumors showing sluggish uptake and clearance of ICG, which was not observable with surface imaging. Similarly, PpIX was quantified, which showed slower kinetics over different time points, and was further compared with the wide-filed imager. These results were further validated through depth measurements in tissue phantoms and model-based interpretation.Conclusion: This fluorescence depth sensing system can be used to sample the interior blood flow characteristics by ICG sensing of tissue as deep as 20 mm into the tissue with sensitivity to kinetics that are superior to surface imaging and may be combined with other imaging modalities such as ultrasound to provide guided deep fluorescence measurements.
Fil: Kulkarni, Madhusudan B.. University of Wisconsin; Estados Unidos
Fil: Reed, Matthew S.. University of Wisconsin; Estados Unidos
Fil: Cao, Xu. University of Wisconsin; Estados Unidos
Fil: García, Héctor Alfredo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina. University of Wisconsin; Estados Unidos
Fil: Ochoa, Marien I.. University of Wisconsin; Estados Unidos
Fil: Jiang, Shudong. Dartmouth College; Estados Unidos
Fil: Hasan, Tayyaba. Harvard Medical School; Estados Unidos
Fil: Doyley, Marvin M.. Rochester Institute of Technology; Estados Unidos
Fil: Pogue, Brian W.. University of Wisconsin; Estados Unidos. Dartmouth College; Estados Unidos
Materia
Tissues
Fluorescence
Depth Sensing
FIber Optics
Kinetics
Cancer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/261261

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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumorsKulkarni, Madhusudan B.Reed, Matthew S.Cao, XuGarcía, Héctor AlfredoOchoa, Marien I.Jiang, ShudongHasan, TayyabaDoyley, Marvin M.Pogue, Brian W.TissuesFluorescenceDepth SensingFIber OpticsKineticsCancerhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Significance: Fluorescence sensing within tissue is an effective tool for tissue characterization; however, the modality and geometry of the image acquisition can alter the observed signal.Aim: We introduce a novel optical fiber-based system capable of measuring two fluorescent contrast agents through 2 cm of tissue with simple passive electronic switching between the excitation light, simultaneously acquiring fluorescence and excitation data. The goal was to quantify indocyanine green (ICG) and protoporphyrin IX (PpIX) within tissue, and the sampling method was compared with wide-field surface imaging to contrast the value of deep sensing versus surface imaging.Approach: This was achieved by choosing filters for specific wavelengths that were mutually exclusive between ICG and PpIX and coupling these filters to two separate detectors, which allows for direct swapping of the excitation and emission channels by switching the on-time of each excitation laser between 780- and 633-nm wavelengths.Results: This system was compared with two non-contact surface imaging systems for both ICG and PpIX, which revealed that the fluorescence depth sensing system was superior in its ability to resolve kinetics differences in deeper tissues that would normally be dominated by strong signals from skin and other surface tissues. Specifically, the system was tested using pancreatic adenocarcinoma tumors injected into murine models, which were imaged at several time points throughout tumor growth to its ∼6-mm diameter. This demonstrated the system’s capability to track longitudinal changes in ICG and PpIX kinetics that result from tumor growth and development, with larger tumors showing sluggish uptake and clearance of ICG, which was not observable with surface imaging. Similarly, PpIX was quantified, which showed slower kinetics over different time points, and was further compared with the wide-filed imager. These results were further validated through depth measurements in tissue phantoms and model-based interpretation.Conclusion: This fluorescence depth sensing system can be used to sample the interior blood flow characteristics by ICG sensing of tissue as deep as 20 mm into the tissue with sensitivity to kinetics that are superior to surface imaging and may be combined with other imaging modalities such as ultrasound to provide guided deep fluorescence measurements.Fil: Kulkarni, Madhusudan B.. University of Wisconsin; Estados UnidosFil: Reed, Matthew S.. University of Wisconsin; Estados UnidosFil: Cao, Xu. University of Wisconsin; Estados UnidosFil: García, Héctor Alfredo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina. University of Wisconsin; Estados UnidosFil: Ochoa, Marien I.. University of Wisconsin; Estados UnidosFil: Jiang, Shudong. Dartmouth College; Estados UnidosFil: Hasan, Tayyaba. Harvard Medical School; Estados UnidosFil: Doyley, Marvin M.. Rochester Institute of Technology; Estados UnidosFil: Pogue, Brian W.. University of Wisconsin; Estados Unidos. Dartmouth College; Estados UnidosSociety of Photo-Optical Instrumentation Engineers2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261261Kulkarni, Madhusudan B.; Reed, Matthew S.; Cao, Xu; García, Héctor Alfredo; Ochoa, Marien I.; et al.; Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors; Society of Photo-Optical Instrumentation Engineers; Journal Of Biomedical Optics; 30; S1; 11-2024; 1-161083-3668CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-30/issue-S1/S13709/Combined-dual-channel-fluorescence-depth-sensing-of-indocyanine-green-and/10.1117/1.JBO.30.S1.S13709.fullinfo:eu-repo/semantics/altIdentifier/doi/10.1117/1.JBO.30.S1.S13709info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:45Zoai:ri.conicet.gov.ar:11336/261261instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:45.443CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors
title Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors
spellingShingle Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors
Kulkarni, Madhusudan B.
Tissues
Fluorescence
Depth Sensing
FIber Optics
Kinetics
Cancer
title_short Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors
title_full Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors
title_fullStr Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors
title_full_unstemmed Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors
title_sort Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors
dc.creator.none.fl_str_mv Kulkarni, Madhusudan B.
Reed, Matthew S.
Cao, Xu
García, Héctor Alfredo
Ochoa, Marien I.
Jiang, Shudong
Hasan, Tayyaba
Doyley, Marvin M.
Pogue, Brian W.
author Kulkarni, Madhusudan B.
author_facet Kulkarni, Madhusudan B.
Reed, Matthew S.
Cao, Xu
García, Héctor Alfredo
Ochoa, Marien I.
Jiang, Shudong
Hasan, Tayyaba
Doyley, Marvin M.
Pogue, Brian W.
author_role author
author2 Reed, Matthew S.
Cao, Xu
García, Héctor Alfredo
Ochoa, Marien I.
Jiang, Shudong
Hasan, Tayyaba
Doyley, Marvin M.
Pogue, Brian W.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tissues
Fluorescence
Depth Sensing
FIber Optics
Kinetics
Cancer
topic Tissues
Fluorescence
Depth Sensing
FIber Optics
Kinetics
Cancer
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Significance: Fluorescence sensing within tissue is an effective tool for tissue characterization; however, the modality and geometry of the image acquisition can alter the observed signal.Aim: We introduce a novel optical fiber-based system capable of measuring two fluorescent contrast agents through 2 cm of tissue with simple passive electronic switching between the excitation light, simultaneously acquiring fluorescence and excitation data. The goal was to quantify indocyanine green (ICG) and protoporphyrin IX (PpIX) within tissue, and the sampling method was compared with wide-field surface imaging to contrast the value of deep sensing versus surface imaging.Approach: This was achieved by choosing filters for specific wavelengths that were mutually exclusive between ICG and PpIX and coupling these filters to two separate detectors, which allows for direct swapping of the excitation and emission channels by switching the on-time of each excitation laser between 780- and 633-nm wavelengths.Results: This system was compared with two non-contact surface imaging systems for both ICG and PpIX, which revealed that the fluorescence depth sensing system was superior in its ability to resolve kinetics differences in deeper tissues that would normally be dominated by strong signals from skin and other surface tissues. Specifically, the system was tested using pancreatic adenocarcinoma tumors injected into murine models, which were imaged at several time points throughout tumor growth to its ∼6-mm diameter. This demonstrated the system’s capability to track longitudinal changes in ICG and PpIX kinetics that result from tumor growth and development, with larger tumors showing sluggish uptake and clearance of ICG, which was not observable with surface imaging. Similarly, PpIX was quantified, which showed slower kinetics over different time points, and was further compared with the wide-filed imager. These results were further validated through depth measurements in tissue phantoms and model-based interpretation.Conclusion: This fluorescence depth sensing system can be used to sample the interior blood flow characteristics by ICG sensing of tissue as deep as 20 mm into the tissue with sensitivity to kinetics that are superior to surface imaging and may be combined with other imaging modalities such as ultrasound to provide guided deep fluorescence measurements.
Fil: Kulkarni, Madhusudan B.. University of Wisconsin; Estados Unidos
Fil: Reed, Matthew S.. University of Wisconsin; Estados Unidos
Fil: Cao, Xu. University of Wisconsin; Estados Unidos
Fil: García, Héctor Alfredo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina. University of Wisconsin; Estados Unidos
Fil: Ochoa, Marien I.. University of Wisconsin; Estados Unidos
Fil: Jiang, Shudong. Dartmouth College; Estados Unidos
Fil: Hasan, Tayyaba. Harvard Medical School; Estados Unidos
Fil: Doyley, Marvin M.. Rochester Institute of Technology; Estados Unidos
Fil: Pogue, Brian W.. University of Wisconsin; Estados Unidos. Dartmouth College; Estados Unidos
description Significance: Fluorescence sensing within tissue is an effective tool for tissue characterization; however, the modality and geometry of the image acquisition can alter the observed signal.Aim: We introduce a novel optical fiber-based system capable of measuring two fluorescent contrast agents through 2 cm of tissue with simple passive electronic switching between the excitation light, simultaneously acquiring fluorescence and excitation data. The goal was to quantify indocyanine green (ICG) and protoporphyrin IX (PpIX) within tissue, and the sampling method was compared with wide-field surface imaging to contrast the value of deep sensing versus surface imaging.Approach: This was achieved by choosing filters for specific wavelengths that were mutually exclusive between ICG and PpIX and coupling these filters to two separate detectors, which allows for direct swapping of the excitation and emission channels by switching the on-time of each excitation laser between 780- and 633-nm wavelengths.Results: This system was compared with two non-contact surface imaging systems for both ICG and PpIX, which revealed that the fluorescence depth sensing system was superior in its ability to resolve kinetics differences in deeper tissues that would normally be dominated by strong signals from skin and other surface tissues. Specifically, the system was tested using pancreatic adenocarcinoma tumors injected into murine models, which were imaged at several time points throughout tumor growth to its ∼6-mm diameter. This demonstrated the system’s capability to track longitudinal changes in ICG and PpIX kinetics that result from tumor growth and development, with larger tumors showing sluggish uptake and clearance of ICG, which was not observable with surface imaging. Similarly, PpIX was quantified, which showed slower kinetics over different time points, and was further compared with the wide-filed imager. These results were further validated through depth measurements in tissue phantoms and model-based interpretation.Conclusion: This fluorescence depth sensing system can be used to sample the interior blood flow characteristics by ICG sensing of tissue as deep as 20 mm into the tissue with sensitivity to kinetics that are superior to surface imaging and may be combined with other imaging modalities such as ultrasound to provide guided deep fluorescence measurements.
publishDate 2024
dc.date.none.fl_str_mv 2024-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/261261
Kulkarni, Madhusudan B.; Reed, Matthew S.; Cao, Xu; García, Héctor Alfredo; Ochoa, Marien I.; et al.; Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors; Society of Photo-Optical Instrumentation Engineers; Journal Of Biomedical Optics; 30; S1; 11-2024; 1-16
1083-3668
CONICET Digital
CONICET
url http://hdl.handle.net/11336/261261
identifier_str_mv Kulkarni, Madhusudan B.; Reed, Matthew S.; Cao, Xu; García, Héctor Alfredo; Ochoa, Marien I.; et al.; Combined dual-channel fluorescence depth sensing of indocyanine green and protoporphyrin IX kinetics in subcutaneous murine tumors; Society of Photo-Optical Instrumentation Engineers; Journal Of Biomedical Optics; 30; S1; 11-2024; 1-16
1083-3668
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-30/issue-S1/S13709/Combined-dual-channel-fluorescence-depth-sensing-of-indocyanine-green-and/10.1117/1.JBO.30.S1.S13709.full
info:eu-repo/semantics/altIdentifier/doi/10.1117/1.JBO.30.S1.S13709
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society of Photo-Optical Instrumentation Engineers
publisher.none.fl_str_mv Society of Photo-Optical Instrumentation Engineers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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