N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor 

Autores
Soto, Daniela; Martini, Claudia Noemí; Frontera, Evelyn Micaela; Montaldo, Laura Andrea; Vila, Maria del Carmen; Calvo, Juan Carlos; Guerra, Liliana Noemi
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: Reports regarding the effects of antioxidants in obesity have been contradictory. AntioxidantN-acetylcysteine is usually considered a nutritional supplement. Our aim is to evaluate bioactivity ofN-acetylcysteine (NAC) on mature adipocytes, which is a close model to in vivo condition.Study Design: In vitro study.Place and Duration of Study: Department of Basic Science (Universidad Nacional de Lujan),Department of Chemical Biology (Universidad de Buenos Aires), CONICET ? INEDES andCONICET ? IQUIBICEN, between March 2017 and June 2019.Methodology: We evaluated the bioactivity of different concentrations of NAC for 5 days (0.01 mMto 5 mM) on fully differentiated 3T3-L1 cells (mature adipocytes).Results: We demonstrated that NAC treatment was not toxic to mature adipocytes. Only 5mM NACinhibited reactive oxygen species production. 5 mM NAC treatment resulted in a 60% decrease incellular triglycerides content and inhibited 70% cholesterol accumulation. We also determined themRNA and protein expression levels of Peroxisome Proliferator-Activated Receptor  as well as,mRNA levels of lipid protein Perilipin in NAC treated adipocytes; we observed that 5mM NACtreatment caused nearly 30% decrease in the expression of these parameters.Conclusion: These results suggest that NAC could avoid lipid accumulation in mature adipocytes;the antioxidant NAC could be beneficial in obesity treatment.
Fil: Soto, Daniela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Martini, Claudia Noemí. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Frontera, Evelyn Micaela. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montaldo, Laura Andrea. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Vila, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Calvo, Juan Carlos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Guerra, Liliana Noemi. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Materia
ADIPOGENESIS
ANTIOXIDANTS
OBESITY
PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/144271
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/144271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor Soto, DanielaMartini, Claudia NoemíFrontera, Evelyn MicaelaMontaldo, Laura AndreaVila, Maria del CarmenCalvo, Juan CarlosGuerra, Liliana NoemiADIPOGENESISANTIOXIDANTSOBESITYPEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: Reports regarding the effects of antioxidants in obesity have been contradictory. AntioxidantN-acetylcysteine is usually considered a nutritional supplement. Our aim is to evaluate bioactivity ofN-acetylcysteine (NAC) on mature adipocytes, which is a close model to in vivo condition.Study Design: In vitro study.Place and Duration of Study: Department of Basic Science (Universidad Nacional de Lujan),Department of Chemical Biology (Universidad de Buenos Aires), CONICET ? INEDES andCONICET ? IQUIBICEN, between March 2017 and June 2019.Methodology: We evaluated the bioactivity of different concentrations of NAC for 5 days (0.01 mMto 5 mM) on fully differentiated 3T3-L1 cells (mature adipocytes).Results: We demonstrated that NAC treatment was not toxic to mature adipocytes. Only 5mM NACinhibited reactive oxygen species production. 5 mM NAC treatment resulted in a 60% decrease incellular triglycerides content and inhibited 70% cholesterol accumulation. We also determined themRNA and protein expression levels of Peroxisome Proliferator-Activated Receptor  as well as,mRNA levels of lipid protein Perilipin in NAC treated adipocytes; we observed that 5mM NACtreatment caused nearly 30% decrease in the expression of these parameters.Conclusion: These results suggest that NAC could avoid lipid accumulation in mature adipocytes;the antioxidant NAC could be beneficial in obesity treatment.Fil: Soto, Daniela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Martini, Claudia Noemí. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Frontera, Evelyn Micaela. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Montaldo, Laura Andrea. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Vila, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Calvo, Juan Carlos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Guerra, Liliana Noemi. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaInternational Journal of Biochemistry Research & Review2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/144271Soto, Daniela; Martini, Claudia Noemí; Frontera, Evelyn Micaela; Montaldo, Laura Andrea; Vila, Maria del Carmen; et al.; N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor ; International Journal of Biochemistry Research & Review; International Journal of Biochemistry Research & Review; 29; 5-2020; 17-292231-086XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.9734/IJBCRR/2020/v29i430182info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:44:21Zoai:ri.conicet.gov.ar:11336/144271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:44:21.549CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor 
title N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor 
spellingShingle N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor 
Soto, Daniela
ADIPOGENESIS
ANTIOXIDANTS
OBESITY
PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA
title_short N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor 
title_full N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor 
title_fullStr N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor 
title_full_unstemmed N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor 
title_sort N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor 
dc.creator.none.fl_str_mv Soto, Daniela
Martini, Claudia Noemí
Frontera, Evelyn Micaela
Montaldo, Laura Andrea
Vila, Maria del Carmen
Calvo, Juan Carlos
Guerra, Liliana Noemi
author Soto, Daniela
author_facet Soto, Daniela
Martini, Claudia Noemí
Frontera, Evelyn Micaela
Montaldo, Laura Andrea
Vila, Maria del Carmen
Calvo, Juan Carlos
Guerra, Liliana Noemi
author_role author
author2 Martini, Claudia Noemí
Frontera, Evelyn Micaela
Montaldo, Laura Andrea
Vila, Maria del Carmen
Calvo, Juan Carlos
Guerra, Liliana Noemi
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ADIPOGENESIS
ANTIOXIDANTS
OBESITY
PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA
topic ADIPOGENESIS
ANTIOXIDANTS
OBESITY
PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aims: Reports regarding the effects of antioxidants in obesity have been contradictory. AntioxidantN-acetylcysteine is usually considered a nutritional supplement. Our aim is to evaluate bioactivity ofN-acetylcysteine (NAC) on mature adipocytes, which is a close model to in vivo condition.Study Design: In vitro study.Place and Duration of Study: Department of Basic Science (Universidad Nacional de Lujan),Department of Chemical Biology (Universidad de Buenos Aires), CONICET ? INEDES andCONICET ? IQUIBICEN, between March 2017 and June 2019.Methodology: We evaluated the bioactivity of different concentrations of NAC for 5 days (0.01 mMto 5 mM) on fully differentiated 3T3-L1 cells (mature adipocytes).Results: We demonstrated that NAC treatment was not toxic to mature adipocytes. Only 5mM NACinhibited reactive oxygen species production. 5 mM NAC treatment resulted in a 60% decrease incellular triglycerides content and inhibited 70% cholesterol accumulation. We also determined themRNA and protein expression levels of Peroxisome Proliferator-Activated Receptor  as well as,mRNA levels of lipid protein Perilipin in NAC treated adipocytes; we observed that 5mM NACtreatment caused nearly 30% decrease in the expression of these parameters.Conclusion: These results suggest that NAC could avoid lipid accumulation in mature adipocytes;the antioxidant NAC could be beneficial in obesity treatment.
Fil: Soto, Daniela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Martini, Claudia Noemí. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Frontera, Evelyn Micaela. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montaldo, Laura Andrea. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Vila, Maria del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Calvo, Juan Carlos. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Guerra, Liliana Noemi. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
description Aims: Reports regarding the effects of antioxidants in obesity have been contradictory. AntioxidantN-acetylcysteine is usually considered a nutritional supplement. Our aim is to evaluate bioactivity ofN-acetylcysteine (NAC) on mature adipocytes, which is a close model to in vivo condition.Study Design: In vitro study.Place and Duration of Study: Department of Basic Science (Universidad Nacional de Lujan),Department of Chemical Biology (Universidad de Buenos Aires), CONICET ? INEDES andCONICET ? IQUIBICEN, between March 2017 and June 2019.Methodology: We evaluated the bioactivity of different concentrations of NAC for 5 days (0.01 mMto 5 mM) on fully differentiated 3T3-L1 cells (mature adipocytes).Results: We demonstrated that NAC treatment was not toxic to mature adipocytes. Only 5mM NACinhibited reactive oxygen species production. 5 mM NAC treatment resulted in a 60% decrease incellular triglycerides content and inhibited 70% cholesterol accumulation. We also determined themRNA and protein expression levels of Peroxisome Proliferator-Activated Receptor  as well as,mRNA levels of lipid protein Perilipin in NAC treated adipocytes; we observed that 5mM NACtreatment caused nearly 30% decrease in the expression of these parameters.Conclusion: These results suggest that NAC could avoid lipid accumulation in mature adipocytes;the antioxidant NAC could be beneficial in obesity treatment.
publishDate 2020
dc.date.none.fl_str_mv 2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/144271
Soto, Daniela; Martini, Claudia Noemí; Frontera, Evelyn Micaela; Montaldo, Laura Andrea; Vila, Maria del Carmen; et al.; N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor ; International Journal of Biochemistry Research & Review; International Journal of Biochemistry Research & Review; 29; 5-2020; 17-29
2231-086X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/144271
identifier_str_mv Soto, Daniela; Martini, Claudia Noemí; Frontera, Evelyn Micaela; Montaldo, Laura Andrea; Vila, Maria del Carmen; et al.; N-Acetylcysteine Inhibits Lipids Production in Mature Adipocytes through the Inhibition of Peroxisome Proliferator-Activated Receptor ; International Journal of Biochemistry Research & Review; International Journal of Biochemistry Research & Review; 29; 5-2020; 17-29
2231-086X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.9734/IJBCRR/2020/v29i430182
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Journal of Biochemistry Research & Review
publisher.none.fl_str_mv International Journal of Biochemistry Research & Review
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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