In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments

Autores
Badel, Jorge L.; Greenshields, David; Piquerez, Sophie J. M.; Rallapalli, Ganashyam; Fabro, Georgina; Ishaque, Naveed; Jones, Jonathan D. G.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genome of the pathogenic oomycete Hyaloperonospora arabidopsidis is predicted to encode at least 134 high-confidence effectors (HaRxL) carrying the RxLR motif implicated in their translocation into plant cells. However, only four avirulence genes (ATR1, ATR13, ATR5, and ATR39) have been isolated. This indicates that identification of HaRxL effectors based on avirulence is low throughput. We aimed at rapidly identifying H. arabidopsidis effectors that contribute to virulence by developing methods to detect and quantify multiple candidates in bacterial mixed infections using either Illumina sequencing or capillary electrophoresis. In these assays, referred to here as in planta effector competition assays, we estimate the contribution to virulence of individual effectors by calculating the abundance of each HaRxL in the bacterial population recovered from leaves 3 days after inoculation relative to abundance in the initial mixed inoculum. We identified HaRxL that enhance Pseudomonas syringae pv. tomato DC3000 growth in some but not all Arabidopsis accessions. Further analysis showed that HaRxLL464, HaRxL75, HaRxL22, HaRxLL441, and HaRxL89 suppress pathogen-associated molecular pattern-triggered immunity (PTI) and localize to different subcellular compartments in Nicotiana benthamiana, providing evidence for a multilayered suppression of PTI by pathogenic oomycetes and molecular probes for the dissection of PTI.
Fil: Badel, Jorge L.. John Innes Institute; Reino Unido
Fil: Greenshields, David. John Innes Institute; Reino Unido
Fil: Piquerez, Sophie J. M.. John Innes Institute; Reino Unido
Fil: Rallapalli, Ganashyam. John Innes Institute; Reino Unido
Fil: Fabro, Georgina. John Innes Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ishaque, Naveed. John Innes Institute; Reino Unido
Fil: Jones, Jonathan D. G.. John Innes Institute; Reino Unido
Materia
EFFECTORS
HYALOPERONOSPORA
ARABIDOPSIS
PSEUDOMONAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25727
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25727
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartmentsBadel, Jorge L.Greenshields, DavidPiquerez, Sophie J. M.Rallapalli, GanashyamFabro, GeorginaIshaque, NaveedJones, Jonathan D. G.EFFECTORSHYALOPERONOSPORAARABIDOPSISPSEUDOMONAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The genome of the pathogenic oomycete Hyaloperonospora arabidopsidis is predicted to encode at least 134 high-confidence effectors (HaRxL) carrying the RxLR motif implicated in their translocation into plant cells. However, only four avirulence genes (ATR1, ATR13, ATR5, and ATR39) have been isolated. This indicates that identification of HaRxL effectors based on avirulence is low throughput. We aimed at rapidly identifying H. arabidopsidis effectors that contribute to virulence by developing methods to detect and quantify multiple candidates in bacterial mixed infections using either Illumina sequencing or capillary electrophoresis. In these assays, referred to here as in planta effector competition assays, we estimate the contribution to virulence of individual effectors by calculating the abundance of each HaRxL in the bacterial population recovered from leaves 3 days after inoculation relative to abundance in the initial mixed inoculum. We identified HaRxL that enhance Pseudomonas syringae pv. tomato DC3000 growth in some but not all Arabidopsis accessions. Further analysis showed that HaRxLL464, HaRxL75, HaRxL22, HaRxLL441, and HaRxL89 suppress pathogen-associated molecular pattern-triggered immunity (PTI) and localize to different subcellular compartments in Nicotiana benthamiana, providing evidence for a multilayered suppression of PTI by pathogenic oomycetes and molecular probes for the dissection of PTI.Fil: Badel, Jorge L.. John Innes Institute; Reino UnidoFil: Greenshields, David. John Innes Institute; Reino UnidoFil: Piquerez, Sophie J. M.. John Innes Institute; Reino UnidoFil: Rallapalli, Ganashyam. John Innes Institute; Reino UnidoFil: Fabro, Georgina. John Innes Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ishaque, Naveed. John Innes Institute; Reino UnidoFil: Jones, Jonathan D. G.. John Innes Institute; Reino UnidoAmerican Phytopathological Society2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25727Badel, Jorge L.; Greenshields, David; Piquerez, Sophie J. M. ; Rallapalli, Ganashyam; Fabro, Georgina; et al.; In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments; American Phytopathological Society; Molecular Plant-Microbe Interactions; 26; 7; 7-2013; 745-7570894-0282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-06-12-0154-Rinfo:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/10.1094/MPMI-06-12-0154-Rinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:41Zoai:ri.conicet.gov.ar:11336/25727instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:42.623CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments
title In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments
spellingShingle In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments
Badel, Jorge L.
EFFECTORS
HYALOPERONOSPORA
ARABIDOPSIS
PSEUDOMONAS
title_short In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments
title_full In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments
title_fullStr In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments
title_full_unstemmed In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments
title_sort In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments
dc.creator.none.fl_str_mv Badel, Jorge L.
Greenshields, David
Piquerez, Sophie J. M.
Rallapalli, Ganashyam
Fabro, Georgina
Ishaque, Naveed
Jones, Jonathan D. G.
author Badel, Jorge L.
author_facet Badel, Jorge L.
Greenshields, David
Piquerez, Sophie J. M.
Rallapalli, Ganashyam
Fabro, Georgina
Ishaque, Naveed
Jones, Jonathan D. G.
author_role author
author2 Greenshields, David
Piquerez, Sophie J. M.
Rallapalli, Ganashyam
Fabro, Georgina
Ishaque, Naveed
Jones, Jonathan D. G.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv EFFECTORS
HYALOPERONOSPORA
ARABIDOPSIS
PSEUDOMONAS
topic EFFECTORS
HYALOPERONOSPORA
ARABIDOPSIS
PSEUDOMONAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The genome of the pathogenic oomycete Hyaloperonospora arabidopsidis is predicted to encode at least 134 high-confidence effectors (HaRxL) carrying the RxLR motif implicated in their translocation into plant cells. However, only four avirulence genes (ATR1, ATR13, ATR5, and ATR39) have been isolated. This indicates that identification of HaRxL effectors based on avirulence is low throughput. We aimed at rapidly identifying H. arabidopsidis effectors that contribute to virulence by developing methods to detect and quantify multiple candidates in bacterial mixed infections using either Illumina sequencing or capillary electrophoresis. In these assays, referred to here as in planta effector competition assays, we estimate the contribution to virulence of individual effectors by calculating the abundance of each HaRxL in the bacterial population recovered from leaves 3 days after inoculation relative to abundance in the initial mixed inoculum. We identified HaRxL that enhance Pseudomonas syringae pv. tomato DC3000 growth in some but not all Arabidopsis accessions. Further analysis showed that HaRxLL464, HaRxL75, HaRxL22, HaRxLL441, and HaRxL89 suppress pathogen-associated molecular pattern-triggered immunity (PTI) and localize to different subcellular compartments in Nicotiana benthamiana, providing evidence for a multilayered suppression of PTI by pathogenic oomycetes and molecular probes for the dissection of PTI.
Fil: Badel, Jorge L.. John Innes Institute; Reino Unido
Fil: Greenshields, David. John Innes Institute; Reino Unido
Fil: Piquerez, Sophie J. M.. John Innes Institute; Reino Unido
Fil: Rallapalli, Ganashyam. John Innes Institute; Reino Unido
Fil: Fabro, Georgina. John Innes Institute; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ishaque, Naveed. John Innes Institute; Reino Unido
Fil: Jones, Jonathan D. G.. John Innes Institute; Reino Unido
description The genome of the pathogenic oomycete Hyaloperonospora arabidopsidis is predicted to encode at least 134 high-confidence effectors (HaRxL) carrying the RxLR motif implicated in their translocation into plant cells. However, only four avirulence genes (ATR1, ATR13, ATR5, and ATR39) have been isolated. This indicates that identification of HaRxL effectors based on avirulence is low throughput. We aimed at rapidly identifying H. arabidopsidis effectors that contribute to virulence by developing methods to detect and quantify multiple candidates in bacterial mixed infections using either Illumina sequencing or capillary electrophoresis. In these assays, referred to here as in planta effector competition assays, we estimate the contribution to virulence of individual effectors by calculating the abundance of each HaRxL in the bacterial population recovered from leaves 3 days after inoculation relative to abundance in the initial mixed inoculum. We identified HaRxL that enhance Pseudomonas syringae pv. tomato DC3000 growth in some but not all Arabidopsis accessions. Further analysis showed that HaRxLL464, HaRxL75, HaRxL22, HaRxLL441, and HaRxL89 suppress pathogen-associated molecular pattern-triggered immunity (PTI) and localize to different subcellular compartments in Nicotiana benthamiana, providing evidence for a multilayered suppression of PTI by pathogenic oomycetes and molecular probes for the dissection of PTI.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25727
Badel, Jorge L.; Greenshields, David; Piquerez, Sophie J. M. ; Rallapalli, Ganashyam; Fabro, Georgina; et al.; In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments; American Phytopathological Society; Molecular Plant-Microbe Interactions; 26; 7; 7-2013; 745-757
0894-0282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25727
identifier_str_mv Badel, Jorge L.; Greenshields, David; Piquerez, Sophie J. M. ; Rallapalli, Ganashyam; Fabro, Georgina; et al.; In Planta effector Competition assays detect Hyaloperonospora arabidopsidis effectors that contribute to virulence and localize to different plant subcellular compartments; American Phytopathological Society; Molecular Plant-Microbe Interactions; 26; 7; 7-2013; 745-757
0894-0282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-06-12-0154-R
info:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/10.1094/MPMI-06-12-0154-R
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268617301819392
score 13.13397

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