Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata

Autores
Cadierno, María Pilar; Dreon, Marcos Sebastian; Heras, Horacio
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The South American freshwater gastropod Pomacea canaliculata is a highly invasive species. In introduced areas, it is a serious crop pest, responsible for great economic loss and ecological damage. It is also a vector of the nematode Angiostrongylus cantonensis that causes human meningoencephalitis. Many aspects and particularly its reproduction have been extensively studied, but little research has been conducted on this species regarding gene expression. To meaningfully interpret quantitative PCR, a powerful technique to develop this kind of study, validation of reference genes is essential but until now has not been undertaken. We selected the female albumen gland for its major role in egg production in order to evaluate the expression stability of the candidate reference genes EF1-α, RPL7, His H3.3, TUBB, 18S RNA, ACTB and GAPDH. Stability was analyzed under different reproductive activity conditions and defined based on three approaches: geNorm, NormFinder and the comparative ΔCt method. NormFinder selected GAPDH and ACTB as the best option to be used for normalization, whereas geNorm and the comparative ΔCt method indicate RPL7, GAPDH, and 18S RNA as the most stable genes under the conditions studied. These results will facilitate reproductive studies, particularly those using qPCR to evaluate factors that may affect fecundity of this conspicuous invasive species.
Fil: Cadierno, María Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Dreon, Marcos Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Heras, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Materia
ALBUMEN GLAND
ANGIOSTRONGYLIASIS
APPLE SNAIL
CROP PEST
INVASIVE SPECIES
PCR
POMACEA
REPRODUCTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96932
Acceder
id CONICETDig_a522e4876c850fdc30c7dc9bb52bdd02
oai_identifier_str oai:ri.conicet.gov.ar:11336/96932
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculataCadierno, María PilarDreon, Marcos SebastianHeras, HoracioALBUMEN GLANDANGIOSTRONGYLIASISAPPLE SNAILCROP PESTINVASIVE SPECIESPCRPOMACEAREPRODUCTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The South American freshwater gastropod Pomacea canaliculata is a highly invasive species. In introduced areas, it is a serious crop pest, responsible for great economic loss and ecological damage. It is also a vector of the nematode Angiostrongylus cantonensis that causes human meningoencephalitis. Many aspects and particularly its reproduction have been extensively studied, but little research has been conducted on this species regarding gene expression. To meaningfully interpret quantitative PCR, a powerful technique to develop this kind of study, validation of reference genes is essential but until now has not been undertaken. We selected the female albumen gland for its major role in egg production in order to evaluate the expression stability of the candidate reference genes EF1-α, RPL7, His H3.3, TUBB, 18S RNA, ACTB and GAPDH. Stability was analyzed under different reproductive activity conditions and defined based on three approaches: geNorm, NormFinder and the comparative ΔCt method. NormFinder selected GAPDH and ACTB as the best option to be used for normalization, whereas geNorm and the comparative ΔCt method indicate RPL7, GAPDH, and 18S RNA as the most stable genes under the conditions studied. These results will facilitate reproductive studies, particularly those using qPCR to evaluate factors that may affect fecundity of this conspicuous invasive species.Fil: Cadierno, María Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Dreon, Marcos Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Heras, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaInstitute of Malacology2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96932Cadierno, María Pilar; Dreon, Marcos Sebastian; Heras, Horacio; Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata; Institute of Malacology; Malacologia; 62; 1; 3-2018; 163-1700076-2997CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4002/040.062.0105info:eu-repo/semantics/altIdentifier/url/https://bioone.org/journals/Malacologia/volume-62/issue-1/040.062.0105/Validation-by-qPCR-of-Reference-Genes-for-Reproductive-Studies-in/10.4002/040.062.0105.shortinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:53Zoai:ri.conicet.gov.ar:11336/96932instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:54.097CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata
title Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata
spellingShingle Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata
Cadierno, María Pilar
ALBUMEN GLAND
ANGIOSTRONGYLIASIS
APPLE SNAIL
CROP PEST
INVASIVE SPECIES
PCR
POMACEA
REPRODUCTION
title_short Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata
title_full Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata
title_fullStr Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata
title_full_unstemmed Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata
title_sort Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata
dc.creator.none.fl_str_mv Cadierno, María Pilar
Dreon, Marcos Sebastian
Heras, Horacio
author Cadierno, María Pilar
author_facet Cadierno, María Pilar
Dreon, Marcos Sebastian
Heras, Horacio
author_role author
author2 Dreon, Marcos Sebastian
Heras, Horacio
author2_role author
author
dc.subject.none.fl_str_mv ALBUMEN GLAND
ANGIOSTRONGYLIASIS
APPLE SNAIL
CROP PEST
INVASIVE SPECIES
PCR
POMACEA
REPRODUCTION
topic ALBUMEN GLAND
ANGIOSTRONGYLIASIS
APPLE SNAIL
CROP PEST
INVASIVE SPECIES
PCR
POMACEA
REPRODUCTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The South American freshwater gastropod Pomacea canaliculata is a highly invasive species. In introduced areas, it is a serious crop pest, responsible for great economic loss and ecological damage. It is also a vector of the nematode Angiostrongylus cantonensis that causes human meningoencephalitis. Many aspects and particularly its reproduction have been extensively studied, but little research has been conducted on this species regarding gene expression. To meaningfully interpret quantitative PCR, a powerful technique to develop this kind of study, validation of reference genes is essential but until now has not been undertaken. We selected the female albumen gland for its major role in egg production in order to evaluate the expression stability of the candidate reference genes EF1-α, RPL7, His H3.3, TUBB, 18S RNA, ACTB and GAPDH. Stability was analyzed under different reproductive activity conditions and defined based on three approaches: geNorm, NormFinder and the comparative ΔCt method. NormFinder selected GAPDH and ACTB as the best option to be used for normalization, whereas geNorm and the comparative ΔCt method indicate RPL7, GAPDH, and 18S RNA as the most stable genes under the conditions studied. These results will facilitate reproductive studies, particularly those using qPCR to evaluate factors that may affect fecundity of this conspicuous invasive species.
Fil: Cadierno, María Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Dreon, Marcos Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Heras, Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
description The South American freshwater gastropod Pomacea canaliculata is a highly invasive species. In introduced areas, it is a serious crop pest, responsible for great economic loss and ecological damage. It is also a vector of the nematode Angiostrongylus cantonensis that causes human meningoencephalitis. Many aspects and particularly its reproduction have been extensively studied, but little research has been conducted on this species regarding gene expression. To meaningfully interpret quantitative PCR, a powerful technique to develop this kind of study, validation of reference genes is essential but until now has not been undertaken. We selected the female albumen gland for its major role in egg production in order to evaluate the expression stability of the candidate reference genes EF1-α, RPL7, His H3.3, TUBB, 18S RNA, ACTB and GAPDH. Stability was analyzed under different reproductive activity conditions and defined based on three approaches: geNorm, NormFinder and the comparative ΔCt method. NormFinder selected GAPDH and ACTB as the best option to be used for normalization, whereas geNorm and the comparative ΔCt method indicate RPL7, GAPDH, and 18S RNA as the most stable genes under the conditions studied. These results will facilitate reproductive studies, particularly those using qPCR to evaluate factors that may affect fecundity of this conspicuous invasive species.
publishDate 2018
dc.date.none.fl_str_mv 2018-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96932
Cadierno, María Pilar; Dreon, Marcos Sebastian; Heras, Horacio; Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata; Institute of Malacology; Malacologia; 62; 1; 3-2018; 163-170
0076-2997
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96932
identifier_str_mv Cadierno, María Pilar; Dreon, Marcos Sebastian; Heras, Horacio; Validation by qPCR of Reference Genes for Reproductive Studies in the Invasive Apple Snail Pomacea canaliculata; Institute of Malacology; Malacologia; 62; 1; 3-2018; 163-170
0076-2997
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4002/040.062.0105
info:eu-repo/semantics/altIdentifier/url/https://bioone.org/journals/Malacologia/volume-62/issue-1/040.062.0105/Validation-by-qPCR-of-Reference-Genes-for-Reproductive-Studies-in/10.4002/040.062.0105.short
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Institute of Malacology
publisher.none.fl_str_mv Institute of Malacology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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