Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models

Autores
Navarrete, Felipe A.; Alvau, Antonio; Lee, Hoi Chang; Levin, Lonny R.; Buck, Jochen; Leon, Patricia Martin De; Santi, Celia M.; Krapf, Dario; Mager, Jesse; Fissore, Rafael A.; Salicioni, Ana M.; Darszon, Alberto; Visconti, Pablo E.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mammalian sperm acquire fertilizing capacity in the female tract in a process called capacitation. At the molecular level, capacitation requires protein kinase A activation, changes in membrane potential and an increase in intracellular calcium. Inhibition of these pathways results in loss of fertilizing ability in vivo and in vitro. We demonstrated that transient incubation of mouse sperm with Ca 2+ ionophore accelerated capacitation and rescued fertilizing capacity in sperm with inactivated PKA function. We now show that a pulse of Ca2+ ionophore induces fertilizing capacity in sperm from infertile CatSper1 (Ca2+ channel), Adcy10 (soluble adenylyl cyclase) and Slo3 (K+ channel) KO mice. In contrast, sperm from infertile mice lacking the Ca 2+ efflux pump PMACA4 were not rescued. These results indicate that a transient increase in intracellular Ca 2+ can overcome genetic infertility in mice and suggest this approach may prove adaptable to rescue sperm function in certain cases of human male infertility.
Fil: Navarrete, Felipe A.. University of Massachusetts; Estados Unidos
Fil: Alvau, Antonio. University of Massachusetts; Estados Unidos
Fil: Lee, Hoi Chang. University of Massachusetts; Estados Unidos
Fil: Levin, Lonny R.. Weill Cornell Medical College; Estados Unidos
Fil: Buck, Jochen. Weill Cornell Medical College; Estados Unidos
Fil: Leon, Patricia Martin De. University of Delaware; Estados Unidos
Fil: Santi, Celia M.. University of Washington; Estados Unidos
Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Mager, Jesse. University of Massachusetts; Estados Unidos
Fil: Fissore, Rafael A.. University of Massachusetts; Estados Unidos
Fil: Salicioni, Ana M.. University of Massachusetts; Estados Unidos
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Visconti, Pablo E.. University of Massachusetts; Estados Unidos
Materia
calcium
fertilization
fertility
ionophore
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60986
Acceder
id CONICETDig_a421fc8436e71e92e653e0d32f54ec94
oai_identifier_str oai:ri.conicet.gov.ar:11336/60986
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse modelsNavarrete, Felipe A.Alvau, AntonioLee, Hoi ChangLevin, Lonny R.Buck, JochenLeon, Patricia Martin DeSanti, Celia M.Krapf, DarioMager, JesseFissore, Rafael A.Salicioni, Ana M.Darszon, AlbertoVisconti, Pablo E.calciumfertilizationfertilityionophorehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mammalian sperm acquire fertilizing capacity in the female tract in a process called capacitation. At the molecular level, capacitation requires protein kinase A activation, changes in membrane potential and an increase in intracellular calcium. Inhibition of these pathways results in loss of fertilizing ability in vivo and in vitro. We demonstrated that transient incubation of mouse sperm with Ca 2+ ionophore accelerated capacitation and rescued fertilizing capacity in sperm with inactivated PKA function. We now show that a pulse of Ca2+ ionophore induces fertilizing capacity in sperm from infertile CatSper1 (Ca2+ channel), Adcy10 (soluble adenylyl cyclase) and Slo3 (K+ channel) KO mice. In contrast, sperm from infertile mice lacking the Ca 2+ efflux pump PMACA4 were not rescued. These results indicate that a transient increase in intracellular Ca 2+ can overcome genetic infertility in mice and suggest this approach may prove adaptable to rescue sperm function in certain cases of human male infertility.Fil: Navarrete, Felipe A.. University of Massachusetts; Estados UnidosFil: Alvau, Antonio. University of Massachusetts; Estados UnidosFil: Lee, Hoi Chang. University of Massachusetts; Estados UnidosFil: Levin, Lonny R.. Weill Cornell Medical College; Estados UnidosFil: Buck, Jochen. Weill Cornell Medical College; Estados UnidosFil: Leon, Patricia Martin De. University of Delaware; Estados UnidosFil: Santi, Celia M.. University of Washington; Estados UnidosFil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Mager, Jesse. University of Massachusetts; Estados UnidosFil: Fissore, Rafael A.. University of Massachusetts; Estados UnidosFil: Salicioni, Ana M.. University of Massachusetts; Estados UnidosFil: Darszon, Alberto. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Visconti, Pablo E.. University of Massachusetts; Estados UnidosNature Publishing Group2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60986Navarrete, Felipe A.; Alvau, Antonio; Lee, Hoi Chang; Levin, Lonny R.; Buck, Jochen; et al.; Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models; Nature Publishing Group; Scientific Reports; 6; 9-2016; 1-9; 335892045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/srep33589info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep33589info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:28:57Zoai:ri.conicet.gov.ar:11336/60986instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:28:57.286CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models
title Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models
spellingShingle Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models
Navarrete, Felipe A.
calcium
fertilization
fertility
ionophore
title_short Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models
title_full Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models
title_fullStr Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models
title_full_unstemmed Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models
title_sort Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models
dc.creator.none.fl_str_mv Navarrete, Felipe A.
Alvau, Antonio
Lee, Hoi Chang
Levin, Lonny R.
Buck, Jochen
Leon, Patricia Martin De
Santi, Celia M.
Krapf, Dario
Mager, Jesse
Fissore, Rafael A.
Salicioni, Ana M.
Darszon, Alberto
Visconti, Pablo E.
author Navarrete, Felipe A.
author_facet Navarrete, Felipe A.
Alvau, Antonio
Lee, Hoi Chang
Levin, Lonny R.
Buck, Jochen
Leon, Patricia Martin De
Santi, Celia M.
Krapf, Dario
Mager, Jesse
Fissore, Rafael A.
Salicioni, Ana M.
Darszon, Alberto
Visconti, Pablo E.
author_role author
author2 Alvau, Antonio
Lee, Hoi Chang
Levin, Lonny R.
Buck, Jochen
Leon, Patricia Martin De
Santi, Celia M.
Krapf, Dario
Mager, Jesse
Fissore, Rafael A.
Salicioni, Ana M.
Darszon, Alberto
Visconti, Pablo E.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv calcium
fertilization
fertility
ionophore
topic calcium
fertilization
fertility
ionophore
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mammalian sperm acquire fertilizing capacity in the female tract in a process called capacitation. At the molecular level, capacitation requires protein kinase A activation, changes in membrane potential and an increase in intracellular calcium. Inhibition of these pathways results in loss of fertilizing ability in vivo and in vitro. We demonstrated that transient incubation of mouse sperm with Ca 2+ ionophore accelerated capacitation and rescued fertilizing capacity in sperm with inactivated PKA function. We now show that a pulse of Ca2+ ionophore induces fertilizing capacity in sperm from infertile CatSper1 (Ca2+ channel), Adcy10 (soluble adenylyl cyclase) and Slo3 (K+ channel) KO mice. In contrast, sperm from infertile mice lacking the Ca 2+ efflux pump PMACA4 were not rescued. These results indicate that a transient increase in intracellular Ca 2+ can overcome genetic infertility in mice and suggest this approach may prove adaptable to rescue sperm function in certain cases of human male infertility.
Fil: Navarrete, Felipe A.. University of Massachusetts; Estados Unidos
Fil: Alvau, Antonio. University of Massachusetts; Estados Unidos
Fil: Lee, Hoi Chang. University of Massachusetts; Estados Unidos
Fil: Levin, Lonny R.. Weill Cornell Medical College; Estados Unidos
Fil: Buck, Jochen. Weill Cornell Medical College; Estados Unidos
Fil: Leon, Patricia Martin De. University of Delaware; Estados Unidos
Fil: Santi, Celia M.. University of Washington; Estados Unidos
Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Mager, Jesse. University of Massachusetts; Estados Unidos
Fil: Fissore, Rafael A.. University of Massachusetts; Estados Unidos
Fil: Salicioni, Ana M.. University of Massachusetts; Estados Unidos
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Visconti, Pablo E.. University of Massachusetts; Estados Unidos
description Mammalian sperm acquire fertilizing capacity in the female tract in a process called capacitation. At the molecular level, capacitation requires protein kinase A activation, changes in membrane potential and an increase in intracellular calcium. Inhibition of these pathways results in loss of fertilizing ability in vivo and in vitro. We demonstrated that transient incubation of mouse sperm with Ca 2+ ionophore accelerated capacitation and rescued fertilizing capacity in sperm with inactivated PKA function. We now show that a pulse of Ca2+ ionophore induces fertilizing capacity in sperm from infertile CatSper1 (Ca2+ channel), Adcy10 (soluble adenylyl cyclase) and Slo3 (K+ channel) KO mice. In contrast, sperm from infertile mice lacking the Ca 2+ efflux pump PMACA4 were not rescued. These results indicate that a transient increase in intracellular Ca 2+ can overcome genetic infertility in mice and suggest this approach may prove adaptable to rescue sperm function in certain cases of human male infertility.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60986
Navarrete, Felipe A.; Alvau, Antonio; Lee, Hoi Chang; Levin, Lonny R.; Buck, Jochen; et al.; Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models; Nature Publishing Group; Scientific Reports; 6; 9-2016; 1-9; 33589
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60986
identifier_str_mv Navarrete, Felipe A.; Alvau, Antonio; Lee, Hoi Chang; Levin, Lonny R.; Buck, Jochen; et al.; Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models; Nature Publishing Group; Scientific Reports; 6; 9-2016; 1-9; 33589
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/srep33589
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep33589
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083429388517376
score 13.22299

Publicaciones similares