Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties

Autores
Schiaffarino, Olivia; Valdivieso González, David; García Pérez, Inés M.; Peñalva, Daniel Alejandro; Almendro Vedia, Víctor G.; Natale, Paolo; López Montero, Iván
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The mitochondrion is an essential organelle enclosed by two membranes whose functionalities depend on their very specific protein and lipid compositions. Proteins from the outer mitochondrial membrane (OMM) are specialized in mitochondrial dynamics and mitophagy, whereas proteins of the inner mitochondrial membrane (IMM) have dedicated functions in cellular respiration and apoptosis. As for lipids, the OMM is enriched in glycerophosphatidyl choline but cardiolipin is exclusively found within the IMM. Though the lipid topology and distribution of the OMM and IMM are known since more than four decades, little is known about the interfacial and dynamic properties of the IMM and OMM lipid extracts. Here we build monolayers, supported bilayers and giant unilamellar vesicles (GUVs) of native OMM and IMM lipids extracts from porcine heart. Additionally, we perform a comparative analysis on the interfacial, phase immiscibility and mechanical properties of both types of extract. Our results show that IMM lipids form more expanded and softer membranes than OMM lipids, allowing a better understanding of the physicochemical and biophysical properties of mitochondrial membranes.
Fil: Schiaffarino, Olivia. Universidad Complutense de Madrid; España
Fil: Valdivieso González, David. Hospital Universitario 12 de Octubre; España. Universidad Complutense de Madrid; España
Fil: García Pérez, Inés M.. Universidad Complutense de Madrid; España
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur; Argentina
Fil: Almendro Vedia, Víctor G.. Universidad Complutense de Madrid; España. Hospital Doce de Octubre, Madrid; España
Fil: Natale, Paolo. Universidad Complutense de Madrid; España. Hospital Universitario 12 de Octubre; España
Fil: López Montero, Iván. Universidad Complutense de Madrid; España. Hospital Universitario 12 de Octubre; España
Materia
FRAP
GIANT UNILAMELLAR VESICLES (GUV)
INNER MITOCHONDRIAL MEMBRANE
LANGMUIR MONOLAYERS
MICROPIPETTES
OUTER MITOCHONDRIAL MEMBRANE
SUPPORTED LIPID BILAYERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201898
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/201898
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mitochondrial membrane models built from native lipid extracts: Interfacial and transport propertiesSchiaffarino, OliviaValdivieso González, DavidGarcía Pérez, Inés M.Peñalva, Daniel AlejandroAlmendro Vedia, Víctor G.Natale, PaoloLópez Montero, IvánFRAPGIANT UNILAMELLAR VESICLES (GUV)INNER MITOCHONDRIAL MEMBRANELANGMUIR MONOLAYERSMICROPIPETTESOUTER MITOCHONDRIAL MEMBRANESUPPORTED LIPID BILAYERShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The mitochondrion is an essential organelle enclosed by two membranes whose functionalities depend on their very specific protein and lipid compositions. Proteins from the outer mitochondrial membrane (OMM) are specialized in mitochondrial dynamics and mitophagy, whereas proteins of the inner mitochondrial membrane (IMM) have dedicated functions in cellular respiration and apoptosis. As for lipids, the OMM is enriched in glycerophosphatidyl choline but cardiolipin is exclusively found within the IMM. Though the lipid topology and distribution of the OMM and IMM are known since more than four decades, little is known about the interfacial and dynamic properties of the IMM and OMM lipid extracts. Here we build monolayers, supported bilayers and giant unilamellar vesicles (GUVs) of native OMM and IMM lipids extracts from porcine heart. Additionally, we perform a comparative analysis on the interfacial, phase immiscibility and mechanical properties of both types of extract. Our results show that IMM lipids form more expanded and softer membranes than OMM lipids, allowing a better understanding of the physicochemical and biophysical properties of mitochondrial membranes.Fil: Schiaffarino, Olivia. Universidad Complutense de Madrid; EspañaFil: Valdivieso González, David. Hospital Universitario 12 de Octubre; España. Universidad Complutense de Madrid; EspañaFil: García Pérez, Inés M.. Universidad Complutense de Madrid; EspañaFil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur; ArgentinaFil: Almendro Vedia, Víctor G.. Universidad Complutense de Madrid; España. Hospital Doce de Octubre, Madrid; EspañaFil: Natale, Paolo. Universidad Complutense de Madrid; España. Hospital Universitario 12 de Octubre; EspañaFil: López Montero, Iván. Universidad Complutense de Madrid; España. Hospital Universitario 12 de Octubre; EspañaFrontiers Media2022-09-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201898Schiaffarino, Olivia; Valdivieso González, David; García Pérez, Inés M.; Peñalva, Daniel Alejandro; Almendro Vedia, Víctor G.; et al.; Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties; Frontiers Media; Frontiers in Molecular Biosciences; 9; 22-9-2022; 1-152296-889XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmolb.2022.910936info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmolb.2022.910936/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:03Zoai:ri.conicet.gov.ar:11336/201898instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:03.821CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
title Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
spellingShingle Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
Schiaffarino, Olivia
FRAP
GIANT UNILAMELLAR VESICLES (GUV)
INNER MITOCHONDRIAL MEMBRANE
LANGMUIR MONOLAYERS
MICROPIPETTES
OUTER MITOCHONDRIAL MEMBRANE
SUPPORTED LIPID BILAYERS
title_short Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
title_full Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
title_fullStr Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
title_full_unstemmed Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
title_sort Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties
dc.creator.none.fl_str_mv Schiaffarino, Olivia
Valdivieso González, David
García Pérez, Inés M.
Peñalva, Daniel Alejandro
Almendro Vedia, Víctor G.
Natale, Paolo
López Montero, Iván
author Schiaffarino, Olivia
author_facet Schiaffarino, Olivia
Valdivieso González, David
García Pérez, Inés M.
Peñalva, Daniel Alejandro
Almendro Vedia, Víctor G.
Natale, Paolo
López Montero, Iván
author_role author
author2 Valdivieso González, David
García Pérez, Inés M.
Peñalva, Daniel Alejandro
Almendro Vedia, Víctor G.
Natale, Paolo
López Montero, Iván
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv FRAP
GIANT UNILAMELLAR VESICLES (GUV)
INNER MITOCHONDRIAL MEMBRANE
LANGMUIR MONOLAYERS
MICROPIPETTES
OUTER MITOCHONDRIAL MEMBRANE
SUPPORTED LIPID BILAYERS
topic FRAP
GIANT UNILAMELLAR VESICLES (GUV)
INNER MITOCHONDRIAL MEMBRANE
LANGMUIR MONOLAYERS
MICROPIPETTES
OUTER MITOCHONDRIAL MEMBRANE
SUPPORTED LIPID BILAYERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The mitochondrion is an essential organelle enclosed by two membranes whose functionalities depend on their very specific protein and lipid compositions. Proteins from the outer mitochondrial membrane (OMM) are specialized in mitochondrial dynamics and mitophagy, whereas proteins of the inner mitochondrial membrane (IMM) have dedicated functions in cellular respiration and apoptosis. As for lipids, the OMM is enriched in glycerophosphatidyl choline but cardiolipin is exclusively found within the IMM. Though the lipid topology and distribution of the OMM and IMM are known since more than four decades, little is known about the interfacial and dynamic properties of the IMM and OMM lipid extracts. Here we build monolayers, supported bilayers and giant unilamellar vesicles (GUVs) of native OMM and IMM lipids extracts from porcine heart. Additionally, we perform a comparative analysis on the interfacial, phase immiscibility and mechanical properties of both types of extract. Our results show that IMM lipids form more expanded and softer membranes than OMM lipids, allowing a better understanding of the physicochemical and biophysical properties of mitochondrial membranes.
Fil: Schiaffarino, Olivia. Universidad Complutense de Madrid; España
Fil: Valdivieso González, David. Hospital Universitario 12 de Octubre; España. Universidad Complutense de Madrid; España
Fil: García Pérez, Inés M.. Universidad Complutense de Madrid; España
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur; Argentina
Fil: Almendro Vedia, Víctor G.. Universidad Complutense de Madrid; España. Hospital Doce de Octubre, Madrid; España
Fil: Natale, Paolo. Universidad Complutense de Madrid; España. Hospital Universitario 12 de Octubre; España
Fil: López Montero, Iván. Universidad Complutense de Madrid; España. Hospital Universitario 12 de Octubre; España
description The mitochondrion is an essential organelle enclosed by two membranes whose functionalities depend on their very specific protein and lipid compositions. Proteins from the outer mitochondrial membrane (OMM) are specialized in mitochondrial dynamics and mitophagy, whereas proteins of the inner mitochondrial membrane (IMM) have dedicated functions in cellular respiration and apoptosis. As for lipids, the OMM is enriched in glycerophosphatidyl choline but cardiolipin is exclusively found within the IMM. Though the lipid topology and distribution of the OMM and IMM are known since more than four decades, little is known about the interfacial and dynamic properties of the IMM and OMM lipid extracts. Here we build monolayers, supported bilayers and giant unilamellar vesicles (GUVs) of native OMM and IMM lipids extracts from porcine heart. Additionally, we perform a comparative analysis on the interfacial, phase immiscibility and mechanical properties of both types of extract. Our results show that IMM lipids form more expanded and softer membranes than OMM lipids, allowing a better understanding of the physicochemical and biophysical properties of mitochondrial membranes.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201898
Schiaffarino, Olivia; Valdivieso González, David; García Pérez, Inés M.; Peñalva, Daniel Alejandro; Almendro Vedia, Víctor G.; et al.; Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties; Frontiers Media; Frontiers in Molecular Biosciences; 9; 22-9-2022; 1-15
2296-889X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201898
identifier_str_mv Schiaffarino, Olivia; Valdivieso González, David; García Pérez, Inés M.; Peñalva, Daniel Alejandro; Almendro Vedia, Víctor G.; et al.; Mitochondrial membrane models built from native lipid extracts: Interfacial and transport properties; Frontiers Media; Frontiers in Molecular Biosciences; 9; 22-9-2022; 1-15
2296-889X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmolb.2022.910936
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmolb.2022.910936/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613732306518016
score 13.070432

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