Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach

Autores
Tavares Campos, Guilherme; Pereira, Felipe Luiz; Morais Barony, Gustavo; Perdigão Rezende, Cristiana; Marques Da Silva, Wanderson; Martins Ferreira de Souza, Gustavo Henrique; Verano Braga, Thiago; De Carvalho Azevedo, Vasco Ariston; Gomes Leal, Carlos Augusto; Pereira Figueiredo, Henrique César
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Streptococcus agalactiae (GBS) is a major pathogen of Nile tilapia, a global commodity of the aquaculture sector. The aims of this study were to evaluate protein expression in the main genotypes of GBS isolated from diseased fishes in Brazil using a label-free shotgun nano-liquid chromatography-ultra definition mass spectrometry (nanoLC-UDMS E ) approach and to compare the differential abundance of proteins identified in strains isolated from GBS-infected fishes and humans. Results: A total of 1070 protein clusters were identified by nanoLC-UDMS E in 5 fish-adapted GBS strains belonging to sequence types ST-260 and ST-927 and the non-typeable (NT) lineage and 1 human GBS strain (ST-23). A total of 1065 protein clusters corresponded to the pan-proteome of fish-adapted GBS strains; 989 of these were identified in all fish-adapted GBS strains (core proteome), and 62 were shared by at least two strains (accessory proteome). Proteins involved in the stress response and in the regulation of gene expression, metabolism and virulence were detected, reflecting the adaptive ability of fish-adapted GBS strains in response to stressor factors that affect bacterial survival in the aquatic environment and bacterial survival and multiplication inside the host cell. Measurement of protein abundance among different hosts showed that 5 and 26 proteins were exclusively found in the human- and fish-adapted GBS strains, respectively; the proteins exclusively identified in fish isolates were mainly related to virulence factors. Furthermore, 215 and 269 proteins were up- and down-regulated, respectively, in the fish-adapted GBS strains in comparison to the human isolate. Conclusions: Our study showed that the core proteome of fish-adapted GBS strains is conserved and demonstrated high similarity of the proteins expressed by fish-adapted strains to the proteome of the human GBS strain. This high degree of proteome conservation of different STs suggests that, a monovalent vaccine may be effective against these variants.
Fil: Tavares Campos, Guilherme. Universidade Federal de Minas Gerais; Brasil
Fil: Pereira, Felipe Luiz. Universidade Federal de Minas Gerais; Brasil
Fil: Morais Barony, Gustavo. Universidade Federal de Minas Gerais; Brasil
Fil: Perdigão Rezende, Cristiana. Universidade Federal de Minas Gerais; Brasil
Fil: Marques Da Silva, Wanderson. Universidade Federal de Minas Gerais; Brasil. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Martins Ferreira de Souza, Gustavo Henrique. No especifíca;
Fil: Verano Braga, Thiago. Universidade Federal de Minas Gerais; Brasil
Fil: De Carvalho Azevedo, Vasco Ariston. Universidade Federal de Minas Gerais; Brasil
Fil: Gomes Leal, Carlos Augusto. Universidade Federal de Minas Gerais; Brasil
Fil: Pereira Figueiredo, Henrique César. Universidade Federal de Minas Gerais; Brasil
Materia
COMPARATIVE PROTEOMICS
FISH
GBS
GENOTYPES
HUMAN
STREPTOCOCCUS AGALACTIAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/149004
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/149004
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approachTavares Campos, GuilhermePereira, Felipe LuizMorais Barony, GustavoPerdigão Rezende, CristianaMarques Da Silva, WandersonMartins Ferreira de Souza, Gustavo HenriqueVerano Braga, ThiagoDe Carvalho Azevedo, Vasco AristonGomes Leal, Carlos AugustoPereira Figueiredo, Henrique CésarCOMPARATIVE PROTEOMICSFISHGBSGENOTYPESHUMANSTREPTOCOCCUS AGALACTIAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Streptococcus agalactiae (GBS) is a major pathogen of Nile tilapia, a global commodity of the aquaculture sector. The aims of this study were to evaluate protein expression in the main genotypes of GBS isolated from diseased fishes in Brazil using a label-free shotgun nano-liquid chromatography-ultra definition mass spectrometry (nanoLC-UDMS E ) approach and to compare the differential abundance of proteins identified in strains isolated from GBS-infected fishes and humans. Results: A total of 1070 protein clusters were identified by nanoLC-UDMS E in 5 fish-adapted GBS strains belonging to sequence types ST-260 and ST-927 and the non-typeable (NT) lineage and 1 human GBS strain (ST-23). A total of 1065 protein clusters corresponded to the pan-proteome of fish-adapted GBS strains; 989 of these were identified in all fish-adapted GBS strains (core proteome), and 62 were shared by at least two strains (accessory proteome). Proteins involved in the stress response and in the regulation of gene expression, metabolism and virulence were detected, reflecting the adaptive ability of fish-adapted GBS strains in response to stressor factors that affect bacterial survival in the aquatic environment and bacterial survival and multiplication inside the host cell. Measurement of protein abundance among different hosts showed that 5 and 26 proteins were exclusively found in the human- and fish-adapted GBS strains, respectively; the proteins exclusively identified in fish isolates were mainly related to virulence factors. Furthermore, 215 and 269 proteins were up- and down-regulated, respectively, in the fish-adapted GBS strains in comparison to the human isolate. Conclusions: Our study showed that the core proteome of fish-adapted GBS strains is conserved and demonstrated high similarity of the proteins expressed by fish-adapted strains to the proteome of the human GBS strain. This high degree of proteome conservation of different STs suggests that, a monovalent vaccine may be effective against these variants.Fil: Tavares Campos, Guilherme. Universidade Federal de Minas Gerais; BrasilFil: Pereira, Felipe Luiz. Universidade Federal de Minas Gerais; BrasilFil: Morais Barony, Gustavo. Universidade Federal de Minas Gerais; BrasilFil: Perdigão Rezende, Cristiana. Universidade Federal de Minas Gerais; BrasilFil: Marques Da Silva, Wanderson. Universidade Federal de Minas Gerais; Brasil. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Martins Ferreira de Souza, Gustavo Henrique. No especifíca;Fil: Verano Braga, Thiago. Universidade Federal de Minas Gerais; BrasilFil: De Carvalho Azevedo, Vasco Ariston. Universidade Federal de Minas Gerais; BrasilFil: Gomes Leal, Carlos Augusto. Universidade Federal de Minas Gerais; BrasilFil: Pereira Figueiredo, Henrique César. Universidade Federal de Minas Gerais; BrasilBioMed Central2019-01-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/149004Tavares Campos, Guilherme; Pereira, Felipe Luiz; Morais Barony, Gustavo; Perdigão Rezende, Cristiana; Marques Da Silva, Wanderson; et al.; Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach; BioMed Central; BMC Genomics; 20; 1; 7-1-2019; 1-221471-2164CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-5423-1info:eu-repo/semantics/altIdentifier/doi/10.1186/s12864-018-5423-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:48Zoai:ri.conicet.gov.ar:11336/149004instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:49.069CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach
title Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach
spellingShingle Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach
Tavares Campos, Guilherme
COMPARATIVE PROTEOMICS
FISH
GBS
GENOTYPES
HUMAN
STREPTOCOCCUS AGALACTIAE
title_short Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach
title_full Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach
title_fullStr Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach
title_full_unstemmed Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach
title_sort Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach
dc.creator.none.fl_str_mv Tavares Campos, Guilherme
Pereira, Felipe Luiz
Morais Barony, Gustavo
Perdigão Rezende, Cristiana
Marques Da Silva, Wanderson
Martins Ferreira de Souza, Gustavo Henrique
Verano Braga, Thiago
De Carvalho Azevedo, Vasco Ariston
Gomes Leal, Carlos Augusto
Pereira Figueiredo, Henrique César
author Tavares Campos, Guilherme
author_facet Tavares Campos, Guilherme
Pereira, Felipe Luiz
Morais Barony, Gustavo
Perdigão Rezende, Cristiana
Marques Da Silva, Wanderson
Martins Ferreira de Souza, Gustavo Henrique
Verano Braga, Thiago
De Carvalho Azevedo, Vasco Ariston
Gomes Leal, Carlos Augusto
Pereira Figueiredo, Henrique César
author_role author
author2 Pereira, Felipe Luiz
Morais Barony, Gustavo
Perdigão Rezende, Cristiana
Marques Da Silva, Wanderson
Martins Ferreira de Souza, Gustavo Henrique
Verano Braga, Thiago
De Carvalho Azevedo, Vasco Ariston
Gomes Leal, Carlos Augusto
Pereira Figueiredo, Henrique César
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COMPARATIVE PROTEOMICS
FISH
GBS
GENOTYPES
HUMAN
STREPTOCOCCUS AGALACTIAE
topic COMPARATIVE PROTEOMICS
FISH
GBS
GENOTYPES
HUMAN
STREPTOCOCCUS AGALACTIAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Streptococcus agalactiae (GBS) is a major pathogen of Nile tilapia, a global commodity of the aquaculture sector. The aims of this study were to evaluate protein expression in the main genotypes of GBS isolated from diseased fishes in Brazil using a label-free shotgun nano-liquid chromatography-ultra definition mass spectrometry (nanoLC-UDMS E ) approach and to compare the differential abundance of proteins identified in strains isolated from GBS-infected fishes and humans. Results: A total of 1070 protein clusters were identified by nanoLC-UDMS E in 5 fish-adapted GBS strains belonging to sequence types ST-260 and ST-927 and the non-typeable (NT) lineage and 1 human GBS strain (ST-23). A total of 1065 protein clusters corresponded to the pan-proteome of fish-adapted GBS strains; 989 of these were identified in all fish-adapted GBS strains (core proteome), and 62 were shared by at least two strains (accessory proteome). Proteins involved in the stress response and in the regulation of gene expression, metabolism and virulence were detected, reflecting the adaptive ability of fish-adapted GBS strains in response to stressor factors that affect bacterial survival in the aquatic environment and bacterial survival and multiplication inside the host cell. Measurement of protein abundance among different hosts showed that 5 and 26 proteins were exclusively found in the human- and fish-adapted GBS strains, respectively; the proteins exclusively identified in fish isolates were mainly related to virulence factors. Furthermore, 215 and 269 proteins were up- and down-regulated, respectively, in the fish-adapted GBS strains in comparison to the human isolate. Conclusions: Our study showed that the core proteome of fish-adapted GBS strains is conserved and demonstrated high similarity of the proteins expressed by fish-adapted strains to the proteome of the human GBS strain. This high degree of proteome conservation of different STs suggests that, a monovalent vaccine may be effective against these variants.
Fil: Tavares Campos, Guilherme. Universidade Federal de Minas Gerais; Brasil
Fil: Pereira, Felipe Luiz. Universidade Federal de Minas Gerais; Brasil
Fil: Morais Barony, Gustavo. Universidade Federal de Minas Gerais; Brasil
Fil: Perdigão Rezende, Cristiana. Universidade Federal de Minas Gerais; Brasil
Fil: Marques Da Silva, Wanderson. Universidade Federal de Minas Gerais; Brasil. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Martins Ferreira de Souza, Gustavo Henrique. No especifíca;
Fil: Verano Braga, Thiago. Universidade Federal de Minas Gerais; Brasil
Fil: De Carvalho Azevedo, Vasco Ariston. Universidade Federal de Minas Gerais; Brasil
Fil: Gomes Leal, Carlos Augusto. Universidade Federal de Minas Gerais; Brasil
Fil: Pereira Figueiredo, Henrique César. Universidade Federal de Minas Gerais; Brasil
description Background: Streptococcus agalactiae (GBS) is a major pathogen of Nile tilapia, a global commodity of the aquaculture sector. The aims of this study were to evaluate protein expression in the main genotypes of GBS isolated from diseased fishes in Brazil using a label-free shotgun nano-liquid chromatography-ultra definition mass spectrometry (nanoLC-UDMS E ) approach and to compare the differential abundance of proteins identified in strains isolated from GBS-infected fishes and humans. Results: A total of 1070 protein clusters were identified by nanoLC-UDMS E in 5 fish-adapted GBS strains belonging to sequence types ST-260 and ST-927 and the non-typeable (NT) lineage and 1 human GBS strain (ST-23). A total of 1065 protein clusters corresponded to the pan-proteome of fish-adapted GBS strains; 989 of these were identified in all fish-adapted GBS strains (core proteome), and 62 were shared by at least two strains (accessory proteome). Proteins involved in the stress response and in the regulation of gene expression, metabolism and virulence were detected, reflecting the adaptive ability of fish-adapted GBS strains in response to stressor factors that affect bacterial survival in the aquatic environment and bacterial survival and multiplication inside the host cell. Measurement of protein abundance among different hosts showed that 5 and 26 proteins were exclusively found in the human- and fish-adapted GBS strains, respectively; the proteins exclusively identified in fish isolates were mainly related to virulence factors. Furthermore, 215 and 269 proteins were up- and down-regulated, respectively, in the fish-adapted GBS strains in comparison to the human isolate. Conclusions: Our study showed that the core proteome of fish-adapted GBS strains is conserved and demonstrated high similarity of the proteins expressed by fish-adapted strains to the proteome of the human GBS strain. This high degree of proteome conservation of different STs suggests that, a monovalent vaccine may be effective against these variants.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/149004
Tavares Campos, Guilherme; Pereira, Felipe Luiz; Morais Barony, Gustavo; Perdigão Rezende, Cristiana; Marques Da Silva, Wanderson; et al.; Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach; BioMed Central; BMC Genomics; 20; 1; 7-1-2019; 1-22
1471-2164
CONICET Digital
CONICET
url http://hdl.handle.net/11336/149004
identifier_str_mv Tavares Campos, Guilherme; Pereira, Felipe Luiz; Morais Barony, Gustavo; Perdigão Rezende, Cristiana; Marques Da Silva, Wanderson; et al.; Delineation of the pan-proteome of fish-pathogenic Streptococcus agalactiae strains using a label-free shotgun approach; BioMed Central; BMC Genomics; 20; 1; 7-1-2019; 1-22
1471-2164
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-018-5423-1
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12864-018-5423-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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