Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes

Autores
Colmano, Guillermo Nicolas; Sánchez, Mariela Eugenia; Turina, Anahi del Valle
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Phospholipid monolayers are often used to emulate biological membrane behavior and interactions with diverse compounds. Yet frequently, these monolayers consist of a single phospholipid or binary mixtures. These monolayers do not share many similarities with biological membranes, for this reason, the aim of this work is to construct Biomimetic Membranes (BM) and test whether they reproduce the behavior of Natural Membranes (NM) and their interaction with an insecticide of sanitary interest, Fipronil. The NMs were synaptosomal membranes of Bos taurus cerebral cortex (NM Bt) and cerebral ganglia membranes of Triatoma infestans nymphs (NMTi). The BMs were assembled with the major lipid components of their respective MN (BMBt and BMTi). Biomimetic Membranes π-A isotherms do not reflect the same behavior of NMs, they are devoided of transition and exhibit a smoother outline. The collapse pressure (πc) of BMBt was quite similar to that of NMBt, yet πc of BMTi was higher than the one observed in NMTi. The compression modulus of both BMs at π =30 mN/m was higher than the one found in NMs, this means that BMs at that π, are more condensed than the natural ones. The presence of fipronil in the subphase, even at low concentrations, causes an increase of ΔV, more evident in NMs. πcutoff essays showed that penetration of fipronil in NMs is only possible up to 34 mN/m, whereas in BMs this value increases to 50 mN/m in BMBt and 44 mN/m in BMTi, suggesting that the presence of proteins in NMs modifies the interaction of fipronil. Fluorescence anisotropy studies showed that BMs behaved similarly to NMs. Fipronil exerted no effect over DPH and TMA-DPH anisotropy values of NMs, but it did affect BMs. EFM images of transferred monolayers show that BMs allow a more homogeneous distribution of the DilC18 fluorescent probe than NMs, but they both exhibit non-defined condensed domains at π above 30 mN/m. Fipronil exerts changes in the distribution of the fluorescent probe in NMs and BMs and this effect was more noticeable at high π values (30 mN/m or higher). In Summary, even though BMs did not emulate their respective NMs perfectly, many parameters were successfully reproduced, and BMTi reproduced its natural counterpart more accurately.
Fil: Colmano, Guillermo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Sánchez, Mariela Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Turina, Anahi del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
XLVIII Reunión Anual de la Sociedad Argentina de Biofísica
San Luis
Argentina
Sociedad Argentina de Biofísica
Materia
BIOMIMETIC MEMBRANES
BOVINE MEMBRANES
TRIATOMINE MEMBRANES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228599
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Design and comparison of biomimetic membranes based on natural bovine and triatomine membranesColmano, Guillermo NicolasSánchez, Mariela EugeniaTurina, Anahi del ValleBIOMIMETIC MEMBRANESBOVINE MEMBRANESTRIATOMINE MEMBRANEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phospholipid monolayers are often used to emulate biological membrane behavior and interactions with diverse compounds. Yet frequently, these monolayers consist of a single phospholipid or binary mixtures. These monolayers do not share many similarities with biological membranes, for this reason, the aim of this work is to construct Biomimetic Membranes (BM) and test whether they reproduce the behavior of Natural Membranes (NM) and their interaction with an insecticide of sanitary interest, Fipronil. The NMs were synaptosomal membranes of Bos taurus cerebral cortex (NM Bt) and cerebral ganglia membranes of Triatoma infestans nymphs (NMTi). The BMs were assembled with the major lipid components of their respective MN (BMBt and BMTi). Biomimetic Membranes π-A isotherms do not reflect the same behavior of NMs, they are devoided of transition and exhibit a smoother outline. The collapse pressure (πc) of BMBt was quite similar to that of NMBt, yet πc of BMTi was higher than the one observed in NMTi. The compression modulus of both BMs at π =30 mN/m was higher than the one found in NMs, this means that BMs at that π, are more condensed than the natural ones. The presence of fipronil in the subphase, even at low concentrations, causes an increase of ΔV, more evident in NMs. πcutoff essays showed that penetration of fipronil in NMs is only possible up to 34 mN/m, whereas in BMs this value increases to 50 mN/m in BMBt and 44 mN/m in BMTi, suggesting that the presence of proteins in NMs modifies the interaction of fipronil. Fluorescence anisotropy studies showed that BMs behaved similarly to NMs. Fipronil exerted no effect over DPH and TMA-DPH anisotropy values of NMs, but it did affect BMs. EFM images of transferred monolayers show that BMs allow a more homogeneous distribution of the DilC18 fluorescent probe than NMs, but they both exhibit non-defined condensed domains at π above 30 mN/m. Fipronil exerts changes in the distribution of the fluorescent probe in NMs and BMs and this effect was more noticeable at high π values (30 mN/m or higher). In Summary, even though BMs did not emulate their respective NMs perfectly, many parameters were successfully reproduced, and BMTi reproduced its natural counterpart more accurately.Fil: Colmano, Guillermo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Sánchez, Mariela Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Turina, Anahi del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaXLVIII Reunión Anual de la Sociedad Argentina de BiofísicaSan LuisArgentinaSociedad Argentina de BiofísicaSociedad Argentina de BiofísicaAndujar, Sebastian AntonioCelej, Maria SoledadAcierno, Juan Pablo2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228599Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes; XLVIII Reunión Anual de la Sociedad Argentina de Biofísica; San Luis; Argentina; 2019; 88-88978-987-27591-7-9CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:56Zoai:ri.conicet.gov.ar:11336/228599instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:56.593CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes
title Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes
spellingShingle Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes
Colmano, Guillermo Nicolas
BIOMIMETIC MEMBRANES
BOVINE MEMBRANES
TRIATOMINE MEMBRANES
title_short Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes
title_full Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes
title_fullStr Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes
title_full_unstemmed Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes
title_sort Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes
dc.creator.none.fl_str_mv Colmano, Guillermo Nicolas
Sánchez, Mariela Eugenia
Turina, Anahi del Valle
author Colmano, Guillermo Nicolas
author_facet Colmano, Guillermo Nicolas
Sánchez, Mariela Eugenia
Turina, Anahi del Valle
author_role author
author2 Sánchez, Mariela Eugenia
Turina, Anahi del Valle
author2_role author
author
dc.contributor.none.fl_str_mv Andujar, Sebastian Antonio
Celej, Maria Soledad
Acierno, Juan Pablo
dc.subject.none.fl_str_mv BIOMIMETIC MEMBRANES
BOVINE MEMBRANES
TRIATOMINE MEMBRANES
topic BIOMIMETIC MEMBRANES
BOVINE MEMBRANES
TRIATOMINE MEMBRANES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phospholipid monolayers are often used to emulate biological membrane behavior and interactions with diverse compounds. Yet frequently, these monolayers consist of a single phospholipid or binary mixtures. These monolayers do not share many similarities with biological membranes, for this reason, the aim of this work is to construct Biomimetic Membranes (BM) and test whether they reproduce the behavior of Natural Membranes (NM) and their interaction with an insecticide of sanitary interest, Fipronil. The NMs were synaptosomal membranes of Bos taurus cerebral cortex (NM Bt) and cerebral ganglia membranes of Triatoma infestans nymphs (NMTi). The BMs were assembled with the major lipid components of their respective MN (BMBt and BMTi). Biomimetic Membranes π-A isotherms do not reflect the same behavior of NMs, they are devoided of transition and exhibit a smoother outline. The collapse pressure (πc) of BMBt was quite similar to that of NMBt, yet πc of BMTi was higher than the one observed in NMTi. The compression modulus of both BMs at π =30 mN/m was higher than the one found in NMs, this means that BMs at that π, are more condensed than the natural ones. The presence of fipronil in the subphase, even at low concentrations, causes an increase of ΔV, more evident in NMs. πcutoff essays showed that penetration of fipronil in NMs is only possible up to 34 mN/m, whereas in BMs this value increases to 50 mN/m in BMBt and 44 mN/m in BMTi, suggesting that the presence of proteins in NMs modifies the interaction of fipronil. Fluorescence anisotropy studies showed that BMs behaved similarly to NMs. Fipronil exerted no effect over DPH and TMA-DPH anisotropy values of NMs, but it did affect BMs. EFM images of transferred monolayers show that BMs allow a more homogeneous distribution of the DilC18 fluorescent probe than NMs, but they both exhibit non-defined condensed domains at π above 30 mN/m. Fipronil exerts changes in the distribution of the fluorescent probe in NMs and BMs and this effect was more noticeable at high π values (30 mN/m or higher). In Summary, even though BMs did not emulate their respective NMs perfectly, many parameters were successfully reproduced, and BMTi reproduced its natural counterpart more accurately.
Fil: Colmano, Guillermo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Sánchez, Mariela Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Turina, Anahi del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
XLVIII Reunión Anual de la Sociedad Argentina de Biofísica
San Luis
Argentina
Sociedad Argentina de Biofísica
description Phospholipid monolayers are often used to emulate biological membrane behavior and interactions with diverse compounds. Yet frequently, these monolayers consist of a single phospholipid or binary mixtures. These monolayers do not share many similarities with biological membranes, for this reason, the aim of this work is to construct Biomimetic Membranes (BM) and test whether they reproduce the behavior of Natural Membranes (NM) and their interaction with an insecticide of sanitary interest, Fipronil. The NMs were synaptosomal membranes of Bos taurus cerebral cortex (NM Bt) and cerebral ganglia membranes of Triatoma infestans nymphs (NMTi). The BMs were assembled with the major lipid components of their respective MN (BMBt and BMTi). Biomimetic Membranes π-A isotherms do not reflect the same behavior of NMs, they are devoided of transition and exhibit a smoother outline. The collapse pressure (πc) of BMBt was quite similar to that of NMBt, yet πc of BMTi was higher than the one observed in NMTi. The compression modulus of both BMs at π =30 mN/m was higher than the one found in NMs, this means that BMs at that π, are more condensed than the natural ones. The presence of fipronil in the subphase, even at low concentrations, causes an increase of ΔV, more evident in NMs. πcutoff essays showed that penetration of fipronil in NMs is only possible up to 34 mN/m, whereas in BMs this value increases to 50 mN/m in BMBt and 44 mN/m in BMTi, suggesting that the presence of proteins in NMs modifies the interaction of fipronil. Fluorescence anisotropy studies showed that BMs behaved similarly to NMs. Fipronil exerted no effect over DPH and TMA-DPH anisotropy values of NMs, but it did affect BMs. EFM images of transferred monolayers show that BMs allow a more homogeneous distribution of the DilC18 fluorescent probe than NMs, but they both exhibit non-defined condensed domains at π above 30 mN/m. Fipronil exerts changes in the distribution of the fluorescent probe in NMs and BMs and this effect was more noticeable at high π values (30 mN/m or higher). In Summary, even though BMs did not emulate their respective NMs perfectly, many parameters were successfully reproduced, and BMTi reproduced its natural counterpart more accurately.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228599
Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes; XLVIII Reunión Anual de la Sociedad Argentina de Biofísica; San Luis; Argentina; 2019; 88-88
978-987-27591-7-9
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228599
identifier_str_mv Design and comparison of biomimetic membranes based on natural bovine and triatomine membranes; XLVIII Reunión Anual de la Sociedad Argentina de Biofísica; San Luis; Argentina; 2019; 88-88
978-987-27591-7-9
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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