Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents

Autores
Quinteros, Melisa de Los Ángeles; Aiassa Martínez, Ivana María; Dalmasso, Pablo Roberto; Paez, Paulina Laura
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Currently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobialproperties of these ones. This study reports the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using a Pseudomonasaeruginosa strain from a reference culture collection. A greenish culture supernatant of P. aeruginosa incubated at 37∘C with a silvernitrate solution for 24 h changed to a yellowish brown color, indicating the formation of Ag-NPs, which was confirmed by UVvisspectroscopy, transmission electron microscopy, and X-ray diffraction. TEM analysis showed spherical and pseudosphericalnanoparticles with a distributed size mainly between 25 and 45 nm, and the XRD pattern revealed the crystalline nature of AgNPs.Also it provides an evaluation of the antimicrobial activity of the biosynthesized Ag-NPs against human pathogenic andopportunistic microorganisms, namely, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Proteus mirabilis,Acinetobacter baumannii, Escherichia coli, P. aeruginosa, and Klebsiella pneumonia. Ag-NPs were found to be bioactive at picomolarconcentration levels showing bactericidal effects against both Gram-positive and Gram-negative bacterial strains. This workdemonstrates the first helpful use of biosynthesized Ag-NPs as broad spectrum bactericidal agents for clinical strains of pathogenicmultidrug-resistant bacteria such as methicillin-resistant S. aureus, A. baumannii, and E. coli. In addition, these Ag-NPs showednegligible cytotoxic effect in human neutrophils suggesting low toxicity to the host.
Fil: Quinteros, Melisa de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Aiassa Martínez, Ivana María. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Dalmasso, Pablo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Paez, Paulina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Materia
Silver Nanoparticles
Biosynthesis
Antibacterial Activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23271
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial AgentsQuinteros, Melisa de Los ÁngelesAiassa Martínez, Ivana MaríaDalmasso, Pablo RobertoPaez, Paulina LauraSilver NanoparticlesBiosynthesisAntibacterial Activityhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Currently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobialproperties of these ones. This study reports the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using a Pseudomonasaeruginosa strain from a reference culture collection. A greenish culture supernatant of P. aeruginosa incubated at 37∘C with a silvernitrate solution for 24 h changed to a yellowish brown color, indicating the formation of Ag-NPs, which was confirmed by UVvisspectroscopy, transmission electron microscopy, and X-ray diffraction. TEM analysis showed spherical and pseudosphericalnanoparticles with a distributed size mainly between 25 and 45 nm, and the XRD pattern revealed the crystalline nature of AgNPs.Also it provides an evaluation of the antimicrobial activity of the biosynthesized Ag-NPs against human pathogenic andopportunistic microorganisms, namely, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Proteus mirabilis,Acinetobacter baumannii, Escherichia coli, P. aeruginosa, and Klebsiella pneumonia. Ag-NPs were found to be bioactive at picomolarconcentration levels showing bactericidal effects against both Gram-positive and Gram-negative bacterial strains. This workdemonstrates the first helpful use of biosynthesized Ag-NPs as broad spectrum bactericidal agents for clinical strains of pathogenicmultidrug-resistant bacteria such as methicillin-resistant S. aureus, A. baumannii, and E. coli. In addition, these Ag-NPs showednegligible cytotoxic effect in human neutrophils suggesting low toxicity to the host.Fil: Quinteros, Melisa de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Aiassa Martínez, Ivana María. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Dalmasso, Pablo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Paez, Paulina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaHindawi Publishing Corporation2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23271Quinteros, Melisa de Los Ángeles; Aiassa Martínez, Ivana María; Dalmasso, Pablo Roberto; Paez, Paulina Laura; Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents; Hindawi Publishing Corporation; International Journal of Biomaterials; 2016; 5-2016; 1-71687-87871687-8795CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ijbm/2016/5971047/info:eu-repo/semantics/altIdentifier/doi/10.1155/2016/5971047info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:47Zoai:ri.conicet.gov.ar:11336/23271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:48.021CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents
title Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents
spellingShingle Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents
Quinteros, Melisa de Los Ángeles
Silver Nanoparticles
Biosynthesis
Antibacterial Activity
title_short Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents
title_full Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents
title_fullStr Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents
title_full_unstemmed Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents
title_sort Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents
dc.creator.none.fl_str_mv Quinteros, Melisa de Los Ángeles
Aiassa Martínez, Ivana María
Dalmasso, Pablo Roberto
Paez, Paulina Laura
author Quinteros, Melisa de Los Ángeles
author_facet Quinteros, Melisa de Los Ángeles
Aiassa Martínez, Ivana María
Dalmasso, Pablo Roberto
Paez, Paulina Laura
author_role author
author2 Aiassa Martínez, Ivana María
Dalmasso, Pablo Roberto
Paez, Paulina Laura
author2_role author
author
author
dc.subject.none.fl_str_mv Silver Nanoparticles
Biosynthesis
Antibacterial Activity
topic Silver Nanoparticles
Biosynthesis
Antibacterial Activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Currently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobialproperties of these ones. This study reports the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using a Pseudomonasaeruginosa strain from a reference culture collection. A greenish culture supernatant of P. aeruginosa incubated at 37∘C with a silvernitrate solution for 24 h changed to a yellowish brown color, indicating the formation of Ag-NPs, which was confirmed by UVvisspectroscopy, transmission electron microscopy, and X-ray diffraction. TEM analysis showed spherical and pseudosphericalnanoparticles with a distributed size mainly between 25 and 45 nm, and the XRD pattern revealed the crystalline nature of AgNPs.Also it provides an evaluation of the antimicrobial activity of the biosynthesized Ag-NPs against human pathogenic andopportunistic microorganisms, namely, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Proteus mirabilis,Acinetobacter baumannii, Escherichia coli, P. aeruginosa, and Klebsiella pneumonia. Ag-NPs were found to be bioactive at picomolarconcentration levels showing bactericidal effects against both Gram-positive and Gram-negative bacterial strains. This workdemonstrates the first helpful use of biosynthesized Ag-NPs as broad spectrum bactericidal agents for clinical strains of pathogenicmultidrug-resistant bacteria such as methicillin-resistant S. aureus, A. baumannii, and E. coli. In addition, these Ag-NPs showednegligible cytotoxic effect in human neutrophils suggesting low toxicity to the host.
Fil: Quinteros, Melisa de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Aiassa Martínez, Ivana María. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Dalmasso, Pablo Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Paez, Paulina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
description Currently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobialproperties of these ones. This study reports the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using a Pseudomonasaeruginosa strain from a reference culture collection. A greenish culture supernatant of P. aeruginosa incubated at 37∘C with a silvernitrate solution for 24 h changed to a yellowish brown color, indicating the formation of Ag-NPs, which was confirmed by UVvisspectroscopy, transmission electron microscopy, and X-ray diffraction. TEM analysis showed spherical and pseudosphericalnanoparticles with a distributed size mainly between 25 and 45 nm, and the XRD pattern revealed the crystalline nature of AgNPs.Also it provides an evaluation of the antimicrobial activity of the biosynthesized Ag-NPs against human pathogenic andopportunistic microorganisms, namely, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Proteus mirabilis,Acinetobacter baumannii, Escherichia coli, P. aeruginosa, and Klebsiella pneumonia. Ag-NPs were found to be bioactive at picomolarconcentration levels showing bactericidal effects against both Gram-positive and Gram-negative bacterial strains. This workdemonstrates the first helpful use of biosynthesized Ag-NPs as broad spectrum bactericidal agents for clinical strains of pathogenicmultidrug-resistant bacteria such as methicillin-resistant S. aureus, A. baumannii, and E. coli. In addition, these Ag-NPs showednegligible cytotoxic effect in human neutrophils suggesting low toxicity to the host.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23271
Quinteros, Melisa de Los Ángeles; Aiassa Martínez, Ivana María; Dalmasso, Pablo Roberto; Paez, Paulina Laura; Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents; Hindawi Publishing Corporation; International Journal of Biomaterials; 2016; 5-2016; 1-7
1687-8787
1687-8795
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23271
identifier_str_mv Quinteros, Melisa de Los Ángeles; Aiassa Martínez, Ivana María; Dalmasso, Pablo Roberto; Paez, Paulina Laura; Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents; Hindawi Publishing Corporation; International Journal of Biomaterials; 2016; 5-2016; 1-7
1687-8787
1687-8795
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ijbm/2016/5971047/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2016/5971047
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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