Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice

Autores
Bruzzone, Ariana; Vanzulli, Silvia; Soldati, Rocío Cinthia; Giulianelli, Sebastian Jesus; Lanari, Claudia Lee Malvina; Luthy, Isabel Alicia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Breast cancer is the most frequent cancer in women. However, in vivo hormone receptor positive and metas­tatic models are scarce. The aim of the pre­­sent manus­cript was to assess if the novel steroid receptor positive hu­man cell li­nes IBH-4, IBH-6 and IBH-7 developed in our laboratory from primary in­fil­trant duc­tal carcinomas are good mo­dels to study in vivo human breast cancer. Cell lines or tumors were inoculated to nude mice in the presence or ab­­sence of hormone supplementation. Growth was analyzed by ANOVA follo­wed by Tukey-Kra­mer´s test. Steroid hormone expression was assessed by con­focal immuno­fluo­res­cen­ce and Western blotting. The histology of the tu­mors was ana­lyzed. IBH-4 and IBH-6 cells were ino­­­cu­lated to nude mice and 100% of the in­jected mi­ce deve­loped tu­mors in the presence or absence of hor­mo­­ne treat­ment, al­though tamo­xifen inhibited growth. IBH-4 and IBH-6 cell lines in vivo gave rise to poorly diffe­­­ren­tiated carci­no­mas with areas of solid growth and sar­co­­ma­toid areas showing no mor­pho­lo­gical signs of epithe­lial differentiation. Distinct fea­tu­res of ma­lignancy were ob­served. IBH-7 tu­mors in animals receiving estradiol were semi-diffe­ren­­tia­ted ade­no­carcinomas. IBH-7 cells grew only in the pre­­sen­ce of estradiol, but even with hormone addition, the tumor take was 20%. The­se tu­mors me­tas­ta­sized to the uterus and lung and vascular tumor emboli we­re evi­dent. IBH-7 tu­mors were invasive and able to break through the pe­ri­­to­neum. As a conclusion, IBH-4 and IBH-6 are good models for studying tu­mor pro­­gres­sion, whereas IBH-7 is a good model for tumor take, being metastatic and stric­tly estro­gen-dependent.
Fil: Bruzzone, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Soldati, Rocío Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Giulianelli, Sebastian Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
Breast Cancer
Human Cell Lines
Hormone Receptors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24475
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24475
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude miceBruzzone, ArianaVanzulli, SilviaSoldati, Rocío CinthiaGiulianelli, Sebastian JesusLanari, Claudia Lee MalvinaLuthy, Isabel AliciaBreast CancerHuman Cell LinesHormone Receptorshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Breast cancer is the most frequent cancer in women. However, in vivo hormone receptor positive and metas­tatic models are scarce. The aim of the pre­­sent manus­cript was to assess if the novel steroid receptor positive hu­man cell li­nes IBH-4, IBH-6 and IBH-7 developed in our laboratory from primary in­fil­trant duc­tal carcinomas are good mo­dels to study in vivo human breast cancer. Cell lines or tumors were inoculated to nude mice in the presence or ab­­sence of hormone supplementation. Growth was analyzed by ANOVA follo­wed by Tukey-Kra­mer´s test. Steroid hormone expression was assessed by con­focal immuno­fluo­res­cen­ce and Western blotting. The histology of the tu­mors was ana­lyzed. IBH-4 and IBH-6 cells were ino­­­cu­lated to nude mice and 100% of the in­jected mi­ce deve­loped tu­mors in the presence or absence of hor­mo­­ne treat­ment, al­though tamo­xifen inhibited growth. IBH-4 and IBH-6 cell lines in vivo gave rise to poorly diffe­­­ren­tiated carci­no­mas with areas of solid growth and sar­co­­ma­toid areas showing no mor­pho­lo­gical signs of epithe­lial differentiation. Distinct fea­tu­res of ma­lignancy were ob­served. IBH-7 tu­mors in animals receiving estradiol were semi-diffe­ren­­tia­ted ade­no­carcinomas. IBH-7 cells grew only in the pre­­sen­ce of estradiol, but even with hormone addition, the tumor take was 20%. The­se tu­mors me­tas­ta­sized to the uterus and lung and vascular tumor emboli we­re evi­dent. IBH-7 tu­mors were invasive and able to break through the pe­ri­­to­neum. As a conclusion, IBH-4 and IBH-6 are good models for studying tu­mor pro­­gres­sion, whereas IBH-7 is a good model for tumor take, being metastatic and stric­tly estro­gen-dependent.Fil: Bruzzone, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Soldati, Rocío Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Giulianelli, Sebastian Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaWiley2009-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24475Bruzzone, Ariana; Vanzulli, Silvia; Soldati, Rocío Cinthia; Giulianelli, Sebastian Jesus; Lanari, Claudia Lee Malvina; et al.; Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice; Wiley; Journal of Cellular Physiology; 219; 2; 2-2009; 477-4840021-95411097-4652CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jcp.21694info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jcp.21694/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:49Zoai:ri.conicet.gov.ar:11336/24475instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:49.779CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice
title Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice
spellingShingle Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice
Bruzzone, Ariana
Breast Cancer
Human Cell Lines
Hormone Receptors
title_short Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice
title_full Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice
title_fullStr Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice
title_full_unstemmed Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice
title_sort Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice
dc.creator.none.fl_str_mv Bruzzone, Ariana
Vanzulli, Silvia
Soldati, Rocío Cinthia
Giulianelli, Sebastian Jesus
Lanari, Claudia Lee Malvina
Luthy, Isabel Alicia
author Bruzzone, Ariana
author_facet Bruzzone, Ariana
Vanzulli, Silvia
Soldati, Rocío Cinthia
Giulianelli, Sebastian Jesus
Lanari, Claudia Lee Malvina
Luthy, Isabel Alicia
author_role author
author2 Vanzulli, Silvia
Soldati, Rocío Cinthia
Giulianelli, Sebastian Jesus
Lanari, Claudia Lee Malvina
Luthy, Isabel Alicia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Breast Cancer
Human Cell Lines
Hormone Receptors
topic Breast Cancer
Human Cell Lines
Hormone Receptors
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Breast cancer is the most frequent cancer in women. However, in vivo hormone receptor positive and metas­tatic models are scarce. The aim of the pre­­sent manus­cript was to assess if the novel steroid receptor positive hu­man cell li­nes IBH-4, IBH-6 and IBH-7 developed in our laboratory from primary in­fil­trant duc­tal carcinomas are good mo­dels to study in vivo human breast cancer. Cell lines or tumors were inoculated to nude mice in the presence or ab­­sence of hormone supplementation. Growth was analyzed by ANOVA follo­wed by Tukey-Kra­mer´s test. Steroid hormone expression was assessed by con­focal immuno­fluo­res­cen­ce and Western blotting. The histology of the tu­mors was ana­lyzed. IBH-4 and IBH-6 cells were ino­­­cu­lated to nude mice and 100% of the in­jected mi­ce deve­loped tu­mors in the presence or absence of hor­mo­­ne treat­ment, al­though tamo­xifen inhibited growth. IBH-4 and IBH-6 cell lines in vivo gave rise to poorly diffe­­­ren­tiated carci­no­mas with areas of solid growth and sar­co­­ma­toid areas showing no mor­pho­lo­gical signs of epithe­lial differentiation. Distinct fea­tu­res of ma­lignancy were ob­served. IBH-7 tu­mors in animals receiving estradiol were semi-diffe­ren­­tia­ted ade­no­carcinomas. IBH-7 cells grew only in the pre­­sen­ce of estradiol, but even with hormone addition, the tumor take was 20%. The­se tu­mors me­tas­ta­sized to the uterus and lung and vascular tumor emboli we­re evi­dent. IBH-7 tu­mors were invasive and able to break through the pe­ri­­to­neum. As a conclusion, IBH-4 and IBH-6 are good models for studying tu­mor pro­­gres­sion, whereas IBH-7 is a good model for tumor take, being metastatic and stric­tly estro­gen-dependent.
Fil: Bruzzone, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Soldati, Rocío Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Giulianelli, Sebastian Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Breast cancer is the most frequent cancer in women. However, in vivo hormone receptor positive and metas­tatic models are scarce. The aim of the pre­­sent manus­cript was to assess if the novel steroid receptor positive hu­man cell li­nes IBH-4, IBH-6 and IBH-7 developed in our laboratory from primary in­fil­trant duc­tal carcinomas are good mo­dels to study in vivo human breast cancer. Cell lines or tumors were inoculated to nude mice in the presence or ab­­sence of hormone supplementation. Growth was analyzed by ANOVA follo­wed by Tukey-Kra­mer´s test. Steroid hormone expression was assessed by con­focal immuno­fluo­res­cen­ce and Western blotting. The histology of the tu­mors was ana­lyzed. IBH-4 and IBH-6 cells were ino­­­cu­lated to nude mice and 100% of the in­jected mi­ce deve­loped tu­mors in the presence or absence of hor­mo­­ne treat­ment, al­though tamo­xifen inhibited growth. IBH-4 and IBH-6 cell lines in vivo gave rise to poorly diffe­­­ren­tiated carci­no­mas with areas of solid growth and sar­co­­ma­toid areas showing no mor­pho­lo­gical signs of epithe­lial differentiation. Distinct fea­tu­res of ma­lignancy were ob­served. IBH-7 tu­mors in animals receiving estradiol were semi-diffe­ren­­tia­ted ade­no­carcinomas. IBH-7 cells grew only in the pre­­sen­ce of estradiol, but even with hormone addition, the tumor take was 20%. The­se tu­mors me­tas­ta­sized to the uterus and lung and vascular tumor emboli we­re evi­dent. IBH-7 tu­mors were invasive and able to break through the pe­ri­­to­neum. As a conclusion, IBH-4 and IBH-6 are good models for studying tu­mor pro­­gres­sion, whereas IBH-7 is a good model for tumor take, being metastatic and stric­tly estro­gen-dependent.
publishDate 2009
dc.date.none.fl_str_mv 2009-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24475
Bruzzone, Ariana; Vanzulli, Silvia; Soldati, Rocío Cinthia; Giulianelli, Sebastian Jesus; Lanari, Claudia Lee Malvina; et al.; Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice; Wiley; Journal of Cellular Physiology; 219; 2; 2-2009; 477-484
0021-9541
1097-4652
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24475
identifier_str_mv Bruzzone, Ariana; Vanzulli, Silvia; Soldati, Rocío Cinthia; Giulianelli, Sebastian Jesus; Lanari, Claudia Lee Malvina; et al.; Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice; Wiley; Journal of Cellular Physiology; 219; 2; 2-2009; 477-484
0021-9541
1097-4652
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jcp.21694
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jcp.21694/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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