Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection

Autores
Valva, Pamela; Gismondi, Maria Ines; Casciato, Paola; Galoppo, Marcela; Lezama, Carol; Galdame, Omar; Gadano, Adrián Carlos; Galoppo, María Cristina; Mullen, Eduardo; de Matteo, Elena Noemí; Preciado, María Victoria
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mechanisms leading to liver damage in chronic hepatitis C (CHC) are being discussed, but both the immune system and the virus are involved. The aim of this study was to evaluate intrahepatic viral infection, apoptosis and portal and periportal/interface infiltrate in paediatric and adult patients to elucidate the pathogenesis of chronic hepatitis C. HCV-infected, activated caspase-3+ and TUNEL+ hepatocytes, as well as total, CD4+, CD8+, Foxp3+ and CD20+ lymphocytes infiltrating portal and periportal/interface tracts were evaluated in 27 paediatric and 32 adult liver samples by immunohistochemistry or immunofluorescence. The number of infected hepatocytes was higher in paediatric than in adult samples (p 0.0078). In children, they correlated with apoptotic hepatocytes (activated caspase-3+r = 0.74, p < 0.0001; TUNEL+r = 0.606, p 0.0017). Also, infected (p = 0.026) and apoptotic hepatocytes (p = 0.03) were associated with the severity of fibrosis. In adults, activated caspase-3+ cell count was increased in severe hepatitis (p = 0.009). Total, CD4+, CD8+ and Foxp3+ lymphocyte count was higher in adult samples (p < 0.05). Paediatric CD8+ cells correlated with infected (r = 0.495, p 0.04) and TUNEL+ hepatocytes (r = 0.474, p = 0.047), while adult ones correlated with activated caspase-3+ hepatocytes (r = 0.387, p 0.04). In adults, CD8+ was associated with hepatitis severity (p < 0.0001) and correlated with inflammatory activity (CD8+ r = 0.639, p 0.0003). HCV, apoptosis and immune response proved to be involved in CHC pathogenesis of both paediatric and adult patients. However, liver injury in paediatric CHC would be largely associated with a viral cytopathic effect mediated by apoptosis, while in adults it would be mainly associated with an exacerbated immune response.
Fil: Valva, Pamela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gismondi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Casciato, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
Fil: Galoppo, Marcela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Lezama, Carol. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Galdame, Omar. Hospital Italiano; Argentina
Fil: Gadano, Adrián Carlos. Hospital Italiano; Argentina
Fil: Galoppo, María Cristina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Mullen, Eduardo. Hospital Italiano; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina
Materia
APOPTOSIS
HEPATITIS C VIRUS
INFECTED HEPATOCYTES
INFILTRATED MICROENVIRONMENT
PATHOGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105118
Acceder
id CONICETDig_a1f37c4e98d4aa49bc8428545f81d5e1
oai_identifier_str oai:ri.conicet.gov.ar:11336/105118
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infectionValva, PamelaGismondi, Maria InesCasciato, PaolaGaloppo, MarcelaLezama, CarolGaldame, OmarGadano, Adrián CarlosGaloppo, María CristinaMullen, Eduardode Matteo, Elena NoemíPreciado, María VictoriaAPOPTOSISHEPATITIS C VIRUSINFECTED HEPATOCYTESINFILTRATED MICROENVIRONMENTPATHOGENESIShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mechanisms leading to liver damage in chronic hepatitis C (CHC) are being discussed, but both the immune system and the virus are involved. The aim of this study was to evaluate intrahepatic viral infection, apoptosis and portal and periportal/interface infiltrate in paediatric and adult patients to elucidate the pathogenesis of chronic hepatitis C. HCV-infected, activated caspase-3+ and TUNEL+ hepatocytes, as well as total, CD4+, CD8+, Foxp3+ and CD20+ lymphocytes infiltrating portal and periportal/interface tracts were evaluated in 27 paediatric and 32 adult liver samples by immunohistochemistry or immunofluorescence. The number of infected hepatocytes was higher in paediatric than in adult samples (p 0.0078). In children, they correlated with apoptotic hepatocytes (activated caspase-3+r = 0.74, p < 0.0001; TUNEL+r = 0.606, p 0.0017). Also, infected (p = 0.026) and apoptotic hepatocytes (p = 0.03) were associated with the severity of fibrosis. In adults, activated caspase-3+ cell count was increased in severe hepatitis (p = 0.009). Total, CD4+, CD8+ and Foxp3+ lymphocyte count was higher in adult samples (p < 0.05). Paediatric CD8+ cells correlated with infected (r = 0.495, p 0.04) and TUNEL+ hepatocytes (r = 0.474, p = 0.047), while adult ones correlated with activated caspase-3+ hepatocytes (r = 0.387, p 0.04). In adults, CD8+ was associated with hepatitis severity (p < 0.0001) and correlated with inflammatory activity (CD8+ r = 0.639, p 0.0003). HCV, apoptosis and immune response proved to be involved in CHC pathogenesis of both paediatric and adult patients. However, liver injury in paediatric CHC would be largely associated with a viral cytopathic effect mediated by apoptosis, while in adults it would be mainly associated with an exacerbated immune response.Fil: Valva, Pamela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gismondi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Casciato, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; ArgentinaFil: Galoppo, Marcela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Lezama, Carol. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Galdame, Omar. Hospital Italiano; ArgentinaFil: Gadano, Adrián Carlos. Hospital Italiano; ArgentinaFil: Galoppo, María Cristina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Mullen, Eduardo. Hospital Italiano; ArgentinaFil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; ArgentinaElsevier2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105118Valva, Pamela; Gismondi, Maria Ines; Casciato, Paola; Galoppo, Marcela; Lezama, Carol; et al.; Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection; Elsevier; Clinical Microbiology And Infection; 20; 12; 12-2014; 998-10091198-743XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(15)60051-9/fulltextinfo:eu-repo/semantics/altIdentifier/doi/10.1111/1469-0691.12728info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:51Zoai:ri.conicet.gov.ar:11336/105118instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:51.945CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection
title Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection
spellingShingle Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection
Valva, Pamela
APOPTOSIS
HEPATITIS C VIRUS
INFECTED HEPATOCYTES
INFILTRATED MICROENVIRONMENT
PATHOGENESIS
title_short Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection
title_full Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection
title_fullStr Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection
title_full_unstemmed Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection
title_sort Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection
dc.creator.none.fl_str_mv Valva, Pamela
Gismondi, Maria Ines
Casciato, Paola
Galoppo, Marcela
Lezama, Carol
Galdame, Omar
Gadano, Adrián Carlos
Galoppo, María Cristina
Mullen, Eduardo
de Matteo, Elena Noemí
Preciado, María Victoria
author Valva, Pamela
author_facet Valva, Pamela
Gismondi, Maria Ines
Casciato, Paola
Galoppo, Marcela
Lezama, Carol
Galdame, Omar
Gadano, Adrián Carlos
Galoppo, María Cristina
Mullen, Eduardo
de Matteo, Elena Noemí
Preciado, María Victoria
author_role author
author2 Gismondi, Maria Ines
Casciato, Paola
Galoppo, Marcela
Lezama, Carol
Galdame, Omar
Gadano, Adrián Carlos
Galoppo, María Cristina
Mullen, Eduardo
de Matteo, Elena Noemí
Preciado, María Victoria
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
HEPATITIS C VIRUS
INFECTED HEPATOCYTES
INFILTRATED MICROENVIRONMENT
PATHOGENESIS
topic APOPTOSIS
HEPATITIS C VIRUS
INFECTED HEPATOCYTES
INFILTRATED MICROENVIRONMENT
PATHOGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mechanisms leading to liver damage in chronic hepatitis C (CHC) are being discussed, but both the immune system and the virus are involved. The aim of this study was to evaluate intrahepatic viral infection, apoptosis and portal and periportal/interface infiltrate in paediatric and adult patients to elucidate the pathogenesis of chronic hepatitis C. HCV-infected, activated caspase-3+ and TUNEL+ hepatocytes, as well as total, CD4+, CD8+, Foxp3+ and CD20+ lymphocytes infiltrating portal and periportal/interface tracts were evaluated in 27 paediatric and 32 adult liver samples by immunohistochemistry or immunofluorescence. The number of infected hepatocytes was higher in paediatric than in adult samples (p 0.0078). In children, they correlated with apoptotic hepatocytes (activated caspase-3+r = 0.74, p < 0.0001; TUNEL+r = 0.606, p 0.0017). Also, infected (p = 0.026) and apoptotic hepatocytes (p = 0.03) were associated with the severity of fibrosis. In adults, activated caspase-3+ cell count was increased in severe hepatitis (p = 0.009). Total, CD4+, CD8+ and Foxp3+ lymphocyte count was higher in adult samples (p < 0.05). Paediatric CD8+ cells correlated with infected (r = 0.495, p 0.04) and TUNEL+ hepatocytes (r = 0.474, p = 0.047), while adult ones correlated with activated caspase-3+ hepatocytes (r = 0.387, p 0.04). In adults, CD8+ was associated with hepatitis severity (p < 0.0001) and correlated with inflammatory activity (CD8+ r = 0.639, p 0.0003). HCV, apoptosis and immune response proved to be involved in CHC pathogenesis of both paediatric and adult patients. However, liver injury in paediatric CHC would be largely associated with a viral cytopathic effect mediated by apoptosis, while in adults it would be mainly associated with an exacerbated immune response.
Fil: Valva, Pamela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gismondi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Casciato, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Italiano; Argentina
Fil: Galoppo, Marcela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Lezama, Carol. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Galdame, Omar. Hospital Italiano; Argentina
Fil: Gadano, Adrián Carlos. Hospital Italiano; Argentina
Fil: Galoppo, María Cristina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Mullen, Eduardo. Hospital Italiano; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Preciado, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Anatomía Patológica; Argentina
description Mechanisms leading to liver damage in chronic hepatitis C (CHC) are being discussed, but both the immune system and the virus are involved. The aim of this study was to evaluate intrahepatic viral infection, apoptosis and portal and periportal/interface infiltrate in paediatric and adult patients to elucidate the pathogenesis of chronic hepatitis C. HCV-infected, activated caspase-3+ and TUNEL+ hepatocytes, as well as total, CD4+, CD8+, Foxp3+ and CD20+ lymphocytes infiltrating portal and periportal/interface tracts were evaluated in 27 paediatric and 32 adult liver samples by immunohistochemistry or immunofluorescence. The number of infected hepatocytes was higher in paediatric than in adult samples (p 0.0078). In children, they correlated with apoptotic hepatocytes (activated caspase-3+r = 0.74, p < 0.0001; TUNEL+r = 0.606, p 0.0017). Also, infected (p = 0.026) and apoptotic hepatocytes (p = 0.03) were associated with the severity of fibrosis. In adults, activated caspase-3+ cell count was increased in severe hepatitis (p = 0.009). Total, CD4+, CD8+ and Foxp3+ lymphocyte count was higher in adult samples (p < 0.05). Paediatric CD8+ cells correlated with infected (r = 0.495, p 0.04) and TUNEL+ hepatocytes (r = 0.474, p = 0.047), while adult ones correlated with activated caspase-3+ hepatocytes (r = 0.387, p 0.04). In adults, CD8+ was associated with hepatitis severity (p < 0.0001) and correlated with inflammatory activity (CD8+ r = 0.639, p 0.0003). HCV, apoptosis and immune response proved to be involved in CHC pathogenesis of both paediatric and adult patients. However, liver injury in paediatric CHC would be largely associated with a viral cytopathic effect mediated by apoptosis, while in adults it would be mainly associated with an exacerbated immune response.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105118
Valva, Pamela; Gismondi, Maria Ines; Casciato, Paola; Galoppo, Marcela; Lezama, Carol; et al.; Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection; Elsevier; Clinical Microbiology And Infection; 20; 12; 12-2014; 998-1009
1198-743X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105118
identifier_str_mv Valva, Pamela; Gismondi, Maria Ines; Casciato, Paola; Galoppo, Marcela; Lezama, Carol; et al.; Distinctive intrahepatic characteristics of paediatric and adult pathogenesis of chronic hepatitis C infection; Elsevier; Clinical Microbiology And Infection; 20; 12; 12-2014; 998-1009
1198-743X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(15)60051-9/fulltext
info:eu-repo/semantics/altIdentifier/doi/10.1111/1469-0691.12728
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614462336663552
score 13.070432

Publicaciones similares