Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives

Autores
Wang, Ping; Tremetsberger, Karin; Urtubey, Estrella; Bernhardt, Karl-Georg
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Premise of the study: We developed microsatellite markers to study clonal growth and interspecific hybridization in the Patagonian and subantarctic plant Hypochaeris incana (Asteraceae) and its closest relatives. Methods and Results: We developed primers for microsatellite loci from 454 sequence reads of genomic DNA of H. incana. We tested them on individuals of H. acaulis, H. hookeri, H. incana, H. palustris, and H. tenuifolia. We selected 15 polymorphic microsatellite loci, which delivered clearly scorable fragments in most or all species. With mean values between 0.7 and 0.8, the expected heterozygosity in populations of H. incana is high. Conclusions: Due to high levels of polymorphism, the developed markers make it possible to distinguish between genets and ramets in H. incana. In some markers, null alleles complicate the scoring of genotypes in tetraploids. All of the developed markers are suitable to study interspecific hybridization among this group of closely related species.
Fil: Wang, Ping. University of Natural Resources and Life Sciences; Austria
Fil: Tremetsberger, Karin. University of Natural Resources and Life Sciences; Austria
Fil: Urtubey, Estrella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina
Fil: Bernhardt, Karl-Georg. University of Natural Resources and Life Sciences; Austria
Materia
HYPOCHAERIS INCANA
MICROSATELLITE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44280
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/44280
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close RelativesWang, PingTremetsberger, KarinUrtubey, EstrellaBernhardt, Karl-GeorgHYPOCHAERIS INCANAMICROSATELLITEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Premise of the study: We developed microsatellite markers to study clonal growth and interspecific hybridization in the Patagonian and subantarctic plant Hypochaeris incana (Asteraceae) and its closest relatives. Methods and Results: We developed primers for microsatellite loci from 454 sequence reads of genomic DNA of H. incana. We tested them on individuals of H. acaulis, H. hookeri, H. incana, H. palustris, and H. tenuifolia. We selected 15 polymorphic microsatellite loci, which delivered clearly scorable fragments in most or all species. With mean values between 0.7 and 0.8, the expected heterozygosity in populations of H. incana is high. Conclusions: Due to high levels of polymorphism, the developed markers make it possible to distinguish between genets and ramets in H. incana. In some markers, null alleles complicate the scoring of genotypes in tetraploids. All of the developed markers are suitable to study interspecific hybridization among this group of closely related species.Fil: Wang, Ping. University of Natural Resources and Life Sciences; AustriaFil: Tremetsberger, Karin. University of Natural Resources and Life Sciences; AustriaFil: Urtubey, Estrella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; ArgentinaFil: Bernhardt, Karl-Georg. University of Natural Resources and Life Sciences; AustriaBotanical Society of America2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44280Wang, Ping; Tremetsberger, Karin; Urtubey, Estrella; Bernhardt, Karl-Georg; Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives; Botanical Society of America; Applications in Plant Sciences; 5; 10-2017; 1-42168-0450CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3732/apps.1700081info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.3732/apps.1700081info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:45Zoai:ri.conicet.gov.ar:11336/44280instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:45.655CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives
title Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives
spellingShingle Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives
Wang, Ping
HYPOCHAERIS INCANA
MICROSATELLITE
title_short Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives
title_full Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives
title_fullStr Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives
title_full_unstemmed Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives
title_sort Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives
dc.creator.none.fl_str_mv Wang, Ping
Tremetsberger, Karin
Urtubey, Estrella
Bernhardt, Karl-Georg
author Wang, Ping
author_facet Wang, Ping
Tremetsberger, Karin
Urtubey, Estrella
Bernhardt, Karl-Georg
author_role author
author2 Tremetsberger, Karin
Urtubey, Estrella
Bernhardt, Karl-Georg
author2_role author
author
author
dc.subject.none.fl_str_mv HYPOCHAERIS INCANA
MICROSATELLITE
topic HYPOCHAERIS INCANA
MICROSATELLITE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Premise of the study: We developed microsatellite markers to study clonal growth and interspecific hybridization in the Patagonian and subantarctic plant Hypochaeris incana (Asteraceae) and its closest relatives. Methods and Results: We developed primers for microsatellite loci from 454 sequence reads of genomic DNA of H. incana. We tested them on individuals of H. acaulis, H. hookeri, H. incana, H. palustris, and H. tenuifolia. We selected 15 polymorphic microsatellite loci, which delivered clearly scorable fragments in most or all species. With mean values between 0.7 and 0.8, the expected heterozygosity in populations of H. incana is high. Conclusions: Due to high levels of polymorphism, the developed markers make it possible to distinguish between genets and ramets in H. incana. In some markers, null alleles complicate the scoring of genotypes in tetraploids. All of the developed markers are suitable to study interspecific hybridization among this group of closely related species.
Fil: Wang, Ping. University of Natural Resources and Life Sciences; Austria
Fil: Tremetsberger, Karin. University of Natural Resources and Life Sciences; Austria
Fil: Urtubey, Estrella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina
Fil: Bernhardt, Karl-Georg. University of Natural Resources and Life Sciences; Austria
description Premise of the study: We developed microsatellite markers to study clonal growth and interspecific hybridization in the Patagonian and subantarctic plant Hypochaeris incana (Asteraceae) and its closest relatives. Methods and Results: We developed primers for microsatellite loci from 454 sequence reads of genomic DNA of H. incana. We tested them on individuals of H. acaulis, H. hookeri, H. incana, H. palustris, and H. tenuifolia. We selected 15 polymorphic microsatellite loci, which delivered clearly scorable fragments in most or all species. With mean values between 0.7 and 0.8, the expected heterozygosity in populations of H. incana is high. Conclusions: Due to high levels of polymorphism, the developed markers make it possible to distinguish between genets and ramets in H. incana. In some markers, null alleles complicate the scoring of genotypes in tetraploids. All of the developed markers are suitable to study interspecific hybridization among this group of closely related species.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44280
Wang, Ping; Tremetsberger, Karin; Urtubey, Estrella; Bernhardt, Karl-Georg; Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives; Botanical Society of America; Applications in Plant Sciences; 5; 10-2017; 1-4
2168-0450
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44280
identifier_str_mv Wang, Ping; Tremetsberger, Karin; Urtubey, Estrella; Bernhardt, Karl-Georg; Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives; Botanical Society of America; Applications in Plant Sciences; 5; 10-2017; 1-4
2168-0450
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3732/apps.1700081
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.3732/apps.1700081
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Botanical Society of America
publisher.none.fl_str_mv Botanical Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268620018679808
score 13.13397

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