In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia

Autores
Vanasco, Virginia; Evelson, Pablo Andrés; Boveris, Alberto Antonio; Alvarez, Silvia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Many studies have reported the occurrence of oxidative stress in different models of sepsis, but no measurements in real time and in non-invasive manner in an acute model of endotoxemia were done, being its mechanism still under debate. In the present work, we have used in situ surface chemiluminescence to evaluate the reactive oxygen species steady-state concentrations and to identify the main chemical species involved in this phenomenon. Experimental endotoxemia provoked a twofold increase in skeletal muscle chemiluminescence (control value: 31±4cps/cm2). The use of cutoff filters and D2O and biacetyl as specific enhancers, indicates that singlet oxygen is the main emitting species in this model. This result closely correlates with elevated TBARS levels, an index of oxidative damage to lipids. Increased NO production and NADPH oxidase activity may support the formation of ONOO-, which in turn may originate HO, an initiator of the lipid oxidation chain. In summary, our data show for the first time that 1O2 is the main chemical and emitting species involved in the mechanism of oxidative stress present in an acute model of endotoxemia. This work provides new insights necessary to understand free radical mechanisms behind endotoxemic syndrome.
Fil: Vanasco, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Alvarez, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Materia
Chemiluminescence
Endotoxemia
Oxidative Stress
Singlet Oxygen
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67619
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemiaVanasco, VirginiaEvelson, Pablo AndrésBoveris, Alberto AntonioAlvarez, SilviaChemiluminescenceEndotoxemiaOxidative StressSinglet Oxygenhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Many studies have reported the occurrence of oxidative stress in different models of sepsis, but no measurements in real time and in non-invasive manner in an acute model of endotoxemia were done, being its mechanism still under debate. In the present work, we have used in situ surface chemiluminescence to evaluate the reactive oxygen species steady-state concentrations and to identify the main chemical species involved in this phenomenon. Experimental endotoxemia provoked a twofold increase in skeletal muscle chemiluminescence (control value: 31±4cps/cm2). The use of cutoff filters and D2O and biacetyl as specific enhancers, indicates that singlet oxygen is the main emitting species in this model. This result closely correlates with elevated TBARS levels, an index of oxidative damage to lipids. Increased NO production and NADPH oxidase activity may support the formation of ONOO-, which in turn may originate HO, an initiator of the lipid oxidation chain. In summary, our data show for the first time that 1O2 is the main chemical and emitting species involved in the mechanism of oxidative stress present in an acute model of endotoxemia. This work provides new insights necessary to understand free radical mechanisms behind endotoxemic syndrome.Fil: Vanasco, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Alvarez, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaElsevier Ireland2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67619Vanasco, Virginia; Evelson, Pablo Andrés; Boveris, Alberto Antonio; Alvarez, Silvia; In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia; Elsevier Ireland; Chemico-biological Interactions; 184; 3; 3-2010; 313-3180009-2797CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbi.2010.01.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009279710000128info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:18Zoai:ri.conicet.gov.ar:11336/67619instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:18.442CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia
title In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia
spellingShingle In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia
Vanasco, Virginia
Chemiluminescence
Endotoxemia
Oxidative Stress
Singlet Oxygen
title_short In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia
title_full In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia
title_fullStr In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia
title_full_unstemmed In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia
title_sort In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia
dc.creator.none.fl_str_mv Vanasco, Virginia
Evelson, Pablo Andrés
Boveris, Alberto Antonio
Alvarez, Silvia
author Vanasco, Virginia
author_facet Vanasco, Virginia
Evelson, Pablo Andrés
Boveris, Alberto Antonio
Alvarez, Silvia
author_role author
author2 Evelson, Pablo Andrés
Boveris, Alberto Antonio
Alvarez, Silvia
author2_role author
author
author
dc.subject.none.fl_str_mv Chemiluminescence
Endotoxemia
Oxidative Stress
Singlet Oxygen
topic Chemiluminescence
Endotoxemia
Oxidative Stress
Singlet Oxygen
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Many studies have reported the occurrence of oxidative stress in different models of sepsis, but no measurements in real time and in non-invasive manner in an acute model of endotoxemia were done, being its mechanism still under debate. In the present work, we have used in situ surface chemiluminescence to evaluate the reactive oxygen species steady-state concentrations and to identify the main chemical species involved in this phenomenon. Experimental endotoxemia provoked a twofold increase in skeletal muscle chemiluminescence (control value: 31±4cps/cm2). The use of cutoff filters and D2O and biacetyl as specific enhancers, indicates that singlet oxygen is the main emitting species in this model. This result closely correlates with elevated TBARS levels, an index of oxidative damage to lipids. Increased NO production and NADPH oxidase activity may support the formation of ONOO-, which in turn may originate HO, an initiator of the lipid oxidation chain. In summary, our data show for the first time that 1O2 is the main chemical and emitting species involved in the mechanism of oxidative stress present in an acute model of endotoxemia. This work provides new insights necessary to understand free radical mechanisms behind endotoxemic syndrome.
Fil: Vanasco, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Boveris, Alberto Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Alvarez, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
description Many studies have reported the occurrence of oxidative stress in different models of sepsis, but no measurements in real time and in non-invasive manner in an acute model of endotoxemia were done, being its mechanism still under debate. In the present work, we have used in situ surface chemiluminescence to evaluate the reactive oxygen species steady-state concentrations and to identify the main chemical species involved in this phenomenon. Experimental endotoxemia provoked a twofold increase in skeletal muscle chemiluminescence (control value: 31±4cps/cm2). The use of cutoff filters and D2O and biacetyl as specific enhancers, indicates that singlet oxygen is the main emitting species in this model. This result closely correlates with elevated TBARS levels, an index of oxidative damage to lipids. Increased NO production and NADPH oxidase activity may support the formation of ONOO-, which in turn may originate HO, an initiator of the lipid oxidation chain. In summary, our data show for the first time that 1O2 is the main chemical and emitting species involved in the mechanism of oxidative stress present in an acute model of endotoxemia. This work provides new insights necessary to understand free radical mechanisms behind endotoxemic syndrome.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67619
Vanasco, Virginia; Evelson, Pablo Andrés; Boveris, Alberto Antonio; Alvarez, Silvia; In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia; Elsevier Ireland; Chemico-biological Interactions; 184; 3; 3-2010; 313-318
0009-2797
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67619
identifier_str_mv Vanasco, Virginia; Evelson, Pablo Andrés; Boveris, Alberto Antonio; Alvarez, Silvia; In situ and real time muscle chemiluminescence determines singlet oxygen involvement in oxidative damage during endotoxemia; Elsevier Ireland; Chemico-biological Interactions; 184; 3; 3-2010; 313-318
0009-2797
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbi.2010.01.011
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009279710000128
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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