Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging

Autores
Tesan, Fiorella Carla; Portillo, Mariano Gastón; Moretton, Marcela Analía; Bernabeu, Ezequiel Adrian; Chiappetta, Diego Andrés; Salgueiro, María Jimena; Zubillaga, Marcela Beatriz
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction In recent years, nanomedicines have raised as a powerful tool to improve prevention, diagnosis and treatment of different pathologies. Among the most well investigated biomaterials, D-α-tocopheryl polyethylene glycol succinate (also known as TPGS) has been on the spot for the last decade. We therefore designed a method to biologically characterize TPGS-based nanomicelles by labeling them with 99mTc. Methods Labeling process was performed by a direct method. The average hydrodynamic diameter of TPGS nanomicelles was measured by dynamic light scattering and radiochemical purity was assessed by thin layer chromatography. Imaging: a dynamic study was performed during the first hour post radioactive micelles administration in a gamma camera (TcO4− was also administered for comparative purposes). Then two static images were acquired in ventral position: 1 h and 12 h post injection. Blood pharmacokinetics of 99mTc-TPGS during 24 h was performed. Results Images revealed whole body biodistribution at an early and delayed time and semiquantification was performed in organs of interest (%Total counts: soft tissue 6.1 ± 0.5; 3.9 ± 0.1, Bone 1.2 ± 0.2; 1.0 ± 0.1, Heart 1.5 ± 0.6; 0.7 ± 0.3, Kidneys 16.6 ± 1.3; 26.5 ± 1.7, Liver 8.6 ± 1.1; 11.1 ± 0.1 for 1 and 12 h post injection respectively). Conclusion This work demonstrated that TPGS based nanomicelles are susceptible to be radiolabeled with 99mTc thus they can be used to perform imaging studies in animal models. Moreover radiolabeling of these delivery nano systems reveals their possibility to be used as diagnostic agents in the near future.
Fil: Tesan, Fiorella Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Portillo, Mariano Gastón. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Moretton, Marcela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Bernabeu, Ezequiel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Chiappetta, Diego Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Salgueiro, María Jimena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Zubillaga, Marcela Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
99MTC
BIODISTRIBUTION
NANOMICELLES
SMALL ANIMAL IMAGING
TPGS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/114060
Acceder
id CONICETDig_a1701b6248494229c16c5500c9fb6210
oai_identifier_str oai:ri.conicet.gov.ar:11336/114060
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imagingTesan, Fiorella CarlaPortillo, Mariano GastónMoretton, Marcela AnalíaBernabeu, Ezequiel AdrianChiappetta, Diego AndrésSalgueiro, María JimenaZubillaga, Marcela Beatriz99MTCBIODISTRIBUTIONNANOMICELLESSMALL ANIMAL IMAGINGTPGShttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Introduction In recent years, nanomedicines have raised as a powerful tool to improve prevention, diagnosis and treatment of different pathologies. Among the most well investigated biomaterials, D-α-tocopheryl polyethylene glycol succinate (also known as TPGS) has been on the spot for the last decade. We therefore designed a method to biologically characterize TPGS-based nanomicelles by labeling them with 99mTc. Methods Labeling process was performed by a direct method. The average hydrodynamic diameter of TPGS nanomicelles was measured by dynamic light scattering and radiochemical purity was assessed by thin layer chromatography. Imaging: a dynamic study was performed during the first hour post radioactive micelles administration in a gamma camera (TcO4− was also administered for comparative purposes). Then two static images were acquired in ventral position: 1 h and 12 h post injection. Blood pharmacokinetics of 99mTc-TPGS during 24 h was performed. Results Images revealed whole body biodistribution at an early and delayed time and semiquantification was performed in organs of interest (%Total counts: soft tissue 6.1 ± 0.5; 3.9 ± 0.1, Bone 1.2 ± 0.2; 1.0 ± 0.1, Heart 1.5 ± 0.6; 0.7 ± 0.3, Kidneys 16.6 ± 1.3; 26.5 ± 1.7, Liver 8.6 ± 1.1; 11.1 ± 0.1 for 1 and 12 h post injection respectively). Conclusion This work demonstrated that TPGS based nanomicelles are susceptible to be radiolabeled with 99mTc thus they can be used to perform imaging studies in animal models. Moreover radiolabeling of these delivery nano systems reveals their possibility to be used as diagnostic agents in the near future.Fil: Tesan, Fiorella Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Portillo, Mariano Gastón. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Moretton, Marcela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Bernabeu, Ezequiel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Chiappetta, Diego Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Salgueiro, María Jimena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Zubillaga, Marcela Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaElsevier Science Inc2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/114060Tesan, Fiorella Carla; Portillo, Mariano Gastón; Moretton, Marcela Analía; Bernabeu, Ezequiel Adrian; Chiappetta, Diego Andrés; et al.; Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging; Elsevier Science Inc; Nuclear Medicine And Biology; 44; 1-2017; 62-680969-8051CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0969805116300348info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nucmedbio.2016.09.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:55:35Zoai:ri.conicet.gov.ar:11336/114060instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:55:35.633CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging
title Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging
spellingShingle Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging
Tesan, Fiorella Carla
99MTC
BIODISTRIBUTION
NANOMICELLES
SMALL ANIMAL IMAGING
TPGS
title_short Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging
title_full Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging
title_fullStr Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging
title_full_unstemmed Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging
title_sort Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging
dc.creator.none.fl_str_mv Tesan, Fiorella Carla
Portillo, Mariano Gastón
Moretton, Marcela Analía
Bernabeu, Ezequiel Adrian
Chiappetta, Diego Andrés
Salgueiro, María Jimena
Zubillaga, Marcela Beatriz
author Tesan, Fiorella Carla
author_facet Tesan, Fiorella Carla
Portillo, Mariano Gastón
Moretton, Marcela Analía
Bernabeu, Ezequiel Adrian
Chiappetta, Diego Andrés
Salgueiro, María Jimena
Zubillaga, Marcela Beatriz
author_role author
author2 Portillo, Mariano Gastón
Moretton, Marcela Analía
Bernabeu, Ezequiel Adrian
Chiappetta, Diego Andrés
Salgueiro, María Jimena
Zubillaga, Marcela Beatriz
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv 99MTC
BIODISTRIBUTION
NANOMICELLES
SMALL ANIMAL IMAGING
TPGS
topic 99MTC
BIODISTRIBUTION
NANOMICELLES
SMALL ANIMAL IMAGING
TPGS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction In recent years, nanomedicines have raised as a powerful tool to improve prevention, diagnosis and treatment of different pathologies. Among the most well investigated biomaterials, D-α-tocopheryl polyethylene glycol succinate (also known as TPGS) has been on the spot for the last decade. We therefore designed a method to biologically characterize TPGS-based nanomicelles by labeling them with 99mTc. Methods Labeling process was performed by a direct method. The average hydrodynamic diameter of TPGS nanomicelles was measured by dynamic light scattering and radiochemical purity was assessed by thin layer chromatography. Imaging: a dynamic study was performed during the first hour post radioactive micelles administration in a gamma camera (TcO4− was also administered for comparative purposes). Then two static images were acquired in ventral position: 1 h and 12 h post injection. Blood pharmacokinetics of 99mTc-TPGS during 24 h was performed. Results Images revealed whole body biodistribution at an early and delayed time and semiquantification was performed in organs of interest (%Total counts: soft tissue 6.1 ± 0.5; 3.9 ± 0.1, Bone 1.2 ± 0.2; 1.0 ± 0.1, Heart 1.5 ± 0.6; 0.7 ± 0.3, Kidneys 16.6 ± 1.3; 26.5 ± 1.7, Liver 8.6 ± 1.1; 11.1 ± 0.1 for 1 and 12 h post injection respectively). Conclusion This work demonstrated that TPGS based nanomicelles are susceptible to be radiolabeled with 99mTc thus they can be used to perform imaging studies in animal models. Moreover radiolabeling of these delivery nano systems reveals their possibility to be used as diagnostic agents in the near future.
Fil: Tesan, Fiorella Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Portillo, Mariano Gastón. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Moretton, Marcela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Bernabeu, Ezequiel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Chiappetta, Diego Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Salgueiro, María Jimena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Zubillaga, Marcela Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description Introduction In recent years, nanomedicines have raised as a powerful tool to improve prevention, diagnosis and treatment of different pathologies. Among the most well investigated biomaterials, D-α-tocopheryl polyethylene glycol succinate (also known as TPGS) has been on the spot for the last decade. We therefore designed a method to biologically characterize TPGS-based nanomicelles by labeling them with 99mTc. Methods Labeling process was performed by a direct method. The average hydrodynamic diameter of TPGS nanomicelles was measured by dynamic light scattering and radiochemical purity was assessed by thin layer chromatography. Imaging: a dynamic study was performed during the first hour post radioactive micelles administration in a gamma camera (TcO4− was also administered for comparative purposes). Then two static images were acquired in ventral position: 1 h and 12 h post injection. Blood pharmacokinetics of 99mTc-TPGS during 24 h was performed. Results Images revealed whole body biodistribution at an early and delayed time and semiquantification was performed in organs of interest (%Total counts: soft tissue 6.1 ± 0.5; 3.9 ± 0.1, Bone 1.2 ± 0.2; 1.0 ± 0.1, Heart 1.5 ± 0.6; 0.7 ± 0.3, Kidneys 16.6 ± 1.3; 26.5 ± 1.7, Liver 8.6 ± 1.1; 11.1 ± 0.1 for 1 and 12 h post injection respectively). Conclusion This work demonstrated that TPGS based nanomicelles are susceptible to be radiolabeled with 99mTc thus they can be used to perform imaging studies in animal models. Moreover radiolabeling of these delivery nano systems reveals their possibility to be used as diagnostic agents in the near future.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/114060
Tesan, Fiorella Carla; Portillo, Mariano Gastón; Moretton, Marcela Analía; Bernabeu, Ezequiel Adrian; Chiappetta, Diego Andrés; et al.; Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging; Elsevier Science Inc; Nuclear Medicine And Biology; 44; 1-2017; 62-68
0969-8051
CONICET Digital
CONICET
url http://hdl.handle.net/11336/114060
identifier_str_mv Tesan, Fiorella Carla; Portillo, Mariano Gastón; Moretton, Marcela Analía; Bernabeu, Ezequiel Adrian; Chiappetta, Diego Andrés; et al.; Radiolabeling and biological characterization of TPGS-based nanomicelles by means of small animal imaging; Elsevier Science Inc; Nuclear Medicine And Biology; 44; 1-2017; 62-68
0969-8051
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0969805116300348
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nucmedbio.2016.09.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083090300010496
score 13.22299

Publicaciones similares