Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds

Autores
Garcia, Cybele; Candurra, Nélida Alicia; Damonte, Elsa Beatriz
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fifteen antiretroviral Zn-finger active compounds with diverse chemical structures, including azoic compounds, hydrazide derivatives, disulfide-based reagents and others, were screened in vitro against Junin virus (JUNV), the etiological agent of Argentine hemorrhagic fever, by a virus yield inhibition assay in Vero cells. Cytotoxicity was evaluated simultaneously by the MTT method. From the total of compounds, three were totally inactive as antivirals, nine presented moderate anti JUNV-activity and three were truly active with EC50 (effective concentration 50%) values in the range 6.5-9.3 µM and selectivity indices greater than 10. The most active inhibitors, named NSC20625, 3-7 and 2-71, demonstrated a broad range of action against arenaviruses, including several attenuated and pathogenic strains of JUNV as well as the antigenically related Tacaribe virus (TACV) and Pichinde virus (PICV). The direct treatment of JUNV and TACV virions with the compounds has shown two types of behavior: the aromatic disulfide NSC20625 was a very potent virucidal agent, whereas the other two compounds exhibited moderate or negligible virus inactivating properties.
Fil: Garcia, Cybele. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Candurra, Nélida Alicia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Damonte, Elsa Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Materia
antiviral
virucidal
arenavirus
zinc finger
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103290
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/103290
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active CompoundsGarcia, CybeleCandurra, Nélida AliciaDamonte, Elsa Beatrizantiviralvirucidalarenaviruszinc fingerhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fifteen antiretroviral Zn-finger active compounds with diverse chemical structures, including azoic compounds, hydrazide derivatives, disulfide-based reagents and others, were screened in vitro against Junin virus (JUNV), the etiological agent of Argentine hemorrhagic fever, by a virus yield inhibition assay in Vero cells. Cytotoxicity was evaluated simultaneously by the MTT method. From the total of compounds, three were totally inactive as antivirals, nine presented moderate anti JUNV-activity and three were truly active with EC50 (effective concentration 50%) values in the range 6.5-9.3 µM and selectivity indices greater than 10. The most active inhibitors, named NSC20625, 3-7 and 2-71, demonstrated a broad range of action against arenaviruses, including several attenuated and pathogenic strains of JUNV as well as the antigenically related Tacaribe virus (TACV) and Pichinde virus (PICV). The direct treatment of JUNV and TACV virions with the compounds has shown two types of behavior: the aromatic disulfide NSC20625 was a very potent virucidal agent, whereas the other two compounds exhibited moderate or negligible virus inactivating properties.Fil: Garcia, Cybele. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Candurra, Nélida Alicia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Damonte, Elsa Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaInternational Medical Press2016-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103290Garcia, Cybele; Candurra, Nélida Alicia; Damonte, Elsa Beatriz; Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds; International Medical Press; Antiviral Chemistry and Chemotherapy; 11; 3; 6-2016; 231-2370956-32022040-2066CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/095632020001100306info:eu-repo/semantics/altIdentifier/doi/10.1177/095632020001100306info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:24Zoai:ri.conicet.gov.ar:11336/103290instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:24.896CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds
title Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds
spellingShingle Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds
Garcia, Cybele
antiviral
virucidal
arenavirus
zinc finger
title_short Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds
title_full Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds
title_fullStr Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds
title_full_unstemmed Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds
title_sort Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds
dc.creator.none.fl_str_mv Garcia, Cybele
Candurra, Nélida Alicia
Damonte, Elsa Beatriz
author Garcia, Cybele
author_facet Garcia, Cybele
Candurra, Nélida Alicia
Damonte, Elsa Beatriz
author_role author
author2 Candurra, Nélida Alicia
Damonte, Elsa Beatriz
author2_role author
author
dc.subject.none.fl_str_mv antiviral
virucidal
arenavirus
zinc finger
topic antiviral
virucidal
arenavirus
zinc finger
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fifteen antiretroviral Zn-finger active compounds with diverse chemical structures, including azoic compounds, hydrazide derivatives, disulfide-based reagents and others, were screened in vitro against Junin virus (JUNV), the etiological agent of Argentine hemorrhagic fever, by a virus yield inhibition assay in Vero cells. Cytotoxicity was evaluated simultaneously by the MTT method. From the total of compounds, three were totally inactive as antivirals, nine presented moderate anti JUNV-activity and three were truly active with EC50 (effective concentration 50%) values in the range 6.5-9.3 µM and selectivity indices greater than 10. The most active inhibitors, named NSC20625, 3-7 and 2-71, demonstrated a broad range of action against arenaviruses, including several attenuated and pathogenic strains of JUNV as well as the antigenically related Tacaribe virus (TACV) and Pichinde virus (PICV). The direct treatment of JUNV and TACV virions with the compounds has shown two types of behavior: the aromatic disulfide NSC20625 was a very potent virucidal agent, whereas the other two compounds exhibited moderate or negligible virus inactivating properties.
Fil: Garcia, Cybele. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Candurra, Nélida Alicia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Damonte, Elsa Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
description Fifteen antiretroviral Zn-finger active compounds with diverse chemical structures, including azoic compounds, hydrazide derivatives, disulfide-based reagents and others, were screened in vitro against Junin virus (JUNV), the etiological agent of Argentine hemorrhagic fever, by a virus yield inhibition assay in Vero cells. Cytotoxicity was evaluated simultaneously by the MTT method. From the total of compounds, three were totally inactive as antivirals, nine presented moderate anti JUNV-activity and three were truly active with EC50 (effective concentration 50%) values in the range 6.5-9.3 µM and selectivity indices greater than 10. The most active inhibitors, named NSC20625, 3-7 and 2-71, demonstrated a broad range of action against arenaviruses, including several attenuated and pathogenic strains of JUNV as well as the antigenically related Tacaribe virus (TACV) and Pichinde virus (PICV). The direct treatment of JUNV and TACV virions with the compounds has shown two types of behavior: the aromatic disulfide NSC20625 was a very potent virucidal agent, whereas the other two compounds exhibited moderate or negligible virus inactivating properties.
publishDate 2016
dc.date.none.fl_str_mv 2016-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103290
Garcia, Cybele; Candurra, Nélida Alicia; Damonte, Elsa Beatriz; Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds; International Medical Press; Antiviral Chemistry and Chemotherapy; 11; 3; 6-2016; 231-237
0956-3202
2040-2066
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103290
identifier_str_mv Garcia, Cybele; Candurra, Nélida Alicia; Damonte, Elsa Beatriz; Antiviral and Virucidal Activities against Arenaviruses of Zinc-Finger Active Compounds; International Medical Press; Antiviral Chemistry and Chemotherapy; 11; 3; 6-2016; 231-237
0956-3202
2040-2066
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/095632020001100306
info:eu-repo/semantics/altIdentifier/doi/10.1177/095632020001100306
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Medical Press
publisher.none.fl_str_mv International Medical Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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