Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism

Autores
Bonjour, Mariel; Benzi Juncos, Oriana Nicole; Maniscalchi Velásquez, Athina del Valle; Conde, Melisa Ailén; Alza, Natalia Paola; Salvador, Gabriela Alejandra
Año de publicación
2025
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In our laboratory, we have previously demonstrated that mild overexpression of α-synuclein in neurons induces a shift in lipid metabolism, promoting lipid droplet and cholesterol accumulation, along with the downregulation of phospholipase D1 (PLD1) and cytoskeletal rearrangements. Additionally, we showed that lipid droplets are essential for neuronal survival under conditions of α-synuclein overexpression. In this study, we aimed to characterize how neuronal α-synuclein overexpression impacts astrocyte biology and metabolism. To this end, we used various glial culture models: mixed primary glia, primary astrocytes, and the C6 astrocytic cell line. These cultures were treated with neuronal conditioned media derived from cells expressing endogenous levels of α-synuclein (e-aSyn CM) or overexpressing α-synuclein (oe-aSyn CM). Both conditions were compared to cultures incubated with regular medium (control). We found that astrocytes exposed to oe-aSyn CM exhibited increased lipid droplets and cholesterol content with the upregulation of diacylglycerol O-acyltransferase expression. Remarkably, these astrocytes also showed an upregulation of PLD1. These metabolic changes were accompanied by actin cytoskeletal rearrangements. Moreover, exposure to oe-aSyn CM also modulated astrocyte proliferative and migratory behavior. Our findings indicate that neuronal α-synuclein overexpression modulates astrocytic lipid metabolism and cytoskeletal architecture and may also affect proliferation and glial scar.
Fil: Bonjour, Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Maniscalchi Velásquez, Athina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LXI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB)
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Materia
Neuron-astrocyte crosstalk
alpha-synuclein
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281051
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid MetabolismBonjour, MarielBenzi Juncos, Oriana NicoleManiscalchi Velásquez, Athina del ValleConde, Melisa AilénAlza, Natalia PaolaSalvador, Gabriela AlejandraNeuron-astrocyte crosstalkalpha-synucleinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In our laboratory, we have previously demonstrated that mild overexpression of α-synuclein in neurons induces a shift in lipid metabolism, promoting lipid droplet and cholesterol accumulation, along with the downregulation of phospholipase D1 (PLD1) and cytoskeletal rearrangements. Additionally, we showed that lipid droplets are essential for neuronal survival under conditions of α-synuclein overexpression. In this study, we aimed to characterize how neuronal α-synuclein overexpression impacts astrocyte biology and metabolism. To this end, we used various glial culture models: mixed primary glia, primary astrocytes, and the C6 astrocytic cell line. These cultures were treated with neuronal conditioned media derived from cells expressing endogenous levels of α-synuclein (e-aSyn CM) or overexpressing α-synuclein (oe-aSyn CM). Both conditions were compared to cultures incubated with regular medium (control). We found that astrocytes exposed to oe-aSyn CM exhibited increased lipid droplets and cholesterol content with the upregulation of diacylglycerol O-acyltransferase expression. Remarkably, these astrocytes also showed an upregulation of PLD1. These metabolic changes were accompanied by actin cytoskeletal rearrangements. Moreover, exposure to oe-aSyn CM also modulated astrocyte proliferative and migratory behavior. Our findings indicate that neuronal α-synuclein overexpression modulates astrocytic lipid metabolism and cytoskeletal architecture and may also affect proliferation and glial scar.Fil: Bonjour, Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Maniscalchi Velásquez, Athina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLXI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB)ArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularSociedad Argentina de Investigaciones en Bioquímica y Biología Molecular2025info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281051Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism; LXI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); Argentina; 2025; 98-98CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/abstracts.pdfinfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/congreso-2025-highlights/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:12:11Zoai:ri.conicet.gov.ar:11336/281051instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:12:11.44CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism
title Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism
spellingShingle Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism
Bonjour, Mariel
Neuron-astrocyte crosstalk
alpha-synuclein
title_short Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism
title_full Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism
title_fullStr Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism
title_full_unstemmed Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism
title_sort Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism
dc.creator.none.fl_str_mv Bonjour, Mariel
Benzi Juncos, Oriana Nicole
Maniscalchi Velásquez, Athina del Valle
Conde, Melisa Ailén
Alza, Natalia Paola
Salvador, Gabriela Alejandra
author Bonjour, Mariel
author_facet Bonjour, Mariel
Benzi Juncos, Oriana Nicole
Maniscalchi Velásquez, Athina del Valle
Conde, Melisa Ailén
Alza, Natalia Paola
Salvador, Gabriela Alejandra
author_role author
author2 Benzi Juncos, Oriana Nicole
Maniscalchi Velásquez, Athina del Valle
Conde, Melisa Ailén
Alza, Natalia Paola
Salvador, Gabriela Alejandra
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Neuron-astrocyte crosstalk
alpha-synuclein
topic Neuron-astrocyte crosstalk
alpha-synuclein
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In our laboratory, we have previously demonstrated that mild overexpression of α-synuclein in neurons induces a shift in lipid metabolism, promoting lipid droplet and cholesterol accumulation, along with the downregulation of phospholipase D1 (PLD1) and cytoskeletal rearrangements. Additionally, we showed that lipid droplets are essential for neuronal survival under conditions of α-synuclein overexpression. In this study, we aimed to characterize how neuronal α-synuclein overexpression impacts astrocyte biology and metabolism. To this end, we used various glial culture models: mixed primary glia, primary astrocytes, and the C6 astrocytic cell line. These cultures were treated with neuronal conditioned media derived from cells expressing endogenous levels of α-synuclein (e-aSyn CM) or overexpressing α-synuclein (oe-aSyn CM). Both conditions were compared to cultures incubated with regular medium (control). We found that astrocytes exposed to oe-aSyn CM exhibited increased lipid droplets and cholesterol content with the upregulation of diacylglycerol O-acyltransferase expression. Remarkably, these astrocytes also showed an upregulation of PLD1. These metabolic changes were accompanied by actin cytoskeletal rearrangements. Moreover, exposure to oe-aSyn CM also modulated astrocyte proliferative and migratory behavior. Our findings indicate that neuronal α-synuclein overexpression modulates astrocytic lipid metabolism and cytoskeletal architecture and may also affect proliferation and glial scar.
Fil: Bonjour, Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Maniscalchi Velásquez, Athina del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LXI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB)
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
description In our laboratory, we have previously demonstrated that mild overexpression of α-synuclein in neurons induces a shift in lipid metabolism, promoting lipid droplet and cholesterol accumulation, along with the downregulation of phospholipase D1 (PLD1) and cytoskeletal rearrangements. Additionally, we showed that lipid droplets are essential for neuronal survival under conditions of α-synuclein overexpression. In this study, we aimed to characterize how neuronal α-synuclein overexpression impacts astrocyte biology and metabolism. To this end, we used various glial culture models: mixed primary glia, primary astrocytes, and the C6 astrocytic cell line. These cultures were treated with neuronal conditioned media derived from cells expressing endogenous levels of α-synuclein (e-aSyn CM) or overexpressing α-synuclein (oe-aSyn CM). Both conditions were compared to cultures incubated with regular medium (control). We found that astrocytes exposed to oe-aSyn CM exhibited increased lipid droplets and cholesterol content with the upregulation of diacylglycerol O-acyltransferase expression. Remarkably, these astrocytes also showed an upregulation of PLD1. These metabolic changes were accompanied by actin cytoskeletal rearrangements. Moreover, exposure to oe-aSyn CM also modulated astrocyte proliferative and migratory behavior. Our findings indicate that neuronal α-synuclein overexpression modulates astrocytic lipid metabolism and cytoskeletal architecture and may also affect proliferation and glial scar.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281051
Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism; LXI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); Argentina; 2025; 98-98
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281051
identifier_str_mv Neuron-Astrocyte Crosstalk: The Never-Ending Story Of Α-Synuclein And Lipid Metabolism; LXI Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research (SAIB); Argentina; 2025; 98-98
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/abstracts.pdf
info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/congreso-2025-highlights/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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