Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)

Autores
Subbotin, Sergei A.; Chitambar, John J.; Chizhov, Vladimir N.; Stanley, Jason D.; Inserra, Renato N.; Doucet, Marcelo Edmundo; Mcclure, Michael; Ye, Weimin; Yeates, George; Mollov, Dimitre S.; Cantalapiedra Navarrete, Carolina; Vovlas, Nicola; Van Den Berg, Esther; Castillo, Pablo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genus Hemicycliophora (Nematoda: Hemicycliophoridae) contains 132 valid species of plant-parasitic nematodes, collectively known as ‘sheath nematodes’. Hemicycliophora spp. are characterized morphologically by a long stylet with rounded basal knobs and a cuticular sheath, present in juvenile and adult stages. Populations of 20 valid and 14 putative species of Hemicycliophora and Loofia from several countries were characterized morphologically using light (LM) and scanning electron microscopy (SEM) and molecularly using the D2-D3 segments of 28S rRNA and internal transcribed spacer (ITS) rRNA gene sequences. LM and SEM observations provided new details on the morphology of these species. PCR-restriction fragment length polymorphisms (PCR-RFLPs) of the D2-D3 of 28S rDNA were proposed for identification of the species. Phylogenetic relationships within populations of 36 species of the genus Hemicycliophora using 102 D2-D3 of 28S rDNA and 97 ITS rRNA gene sequences as inferred from Bayesian analysis are reconstructed and discussed. Ancestral state reconstructions of diagnostic characters (body and stylet length, number of body annuli, shape of vulval lip and tail), using maximum parsimony and Bayesian inference, revealed that none of the traits are individually reliable characters for classifying the studied sheath nematode. The Shimodaira–Hasegawa test rejected the validity of the genus Loofia. This is the most complete phylogenetic analysis of Hemicycliophora species conducted so far.
Fil: Subbotin, Sergei A.. California Department of Food and Agriculture; Estados Unidos. Institute of Ecology and Evolution of the Russian Academy of Sciences; Rusia
Fil: Chitambar, John J.. California Department of Food and Agriculture; Estados Unidos
Fil: Chizhov, Vladimir N.. Institute of Ecology and Evolution of the Russian Academy of Sciences; Rusia
Fil: Stanley, Jason D.. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Inserra, Renato N.. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Doucet, Marcelo Edmundo. Universidad Nacional de Cordoba. Facultad de Cs.exactas Fisicas y Naturales. Centro de Zoologia Aplicada; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; Argentina
Fil: Mcclure, Michael. University Of Arizona; Estados Unidos
Fil: Ye, Weimin. North Carolina Department of Agriculture & Consumer Services; Estados Unidos
Fil: Yeates, George.
Fil: Mollov, Dimitre S.. University Of Minnesota; Estados Unidos
Fil: Cantalapiedra Navarrete, Carolina. Consejo Superior de Investigaciones Científicas. Instituto de Agricultura Sostenible; España
Fil: Vovlas, Nicola. Istituto per la Protezione delle Piante; Italia
Fil: Van Den Berg, Esther. ARC-Plant Protection Research Institute; Sudáfrica
Fil: Castillo, Pablo. Consejo Superior de Investigaciones Científicas. Instituto de Agricultura Sostenible; España
Materia
Ancestral State Reconstructions
Bayesian Inference
Cryptic Species
D2-D3
Pcr-Its-Rflp
Rdna
Sheath Nematodes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8002
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8002
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)Subbotin, Sergei A.Chitambar, John J.Chizhov, Vladimir N.Stanley, Jason D.Inserra, Renato N.Doucet, Marcelo EdmundoMcclure, MichaelYe, WeiminYeates, GeorgeMollov, Dimitre S.Cantalapiedra Navarrete, CarolinaVovlas, NicolaVan Den Berg, EstherCastillo, PabloAncestral State ReconstructionsBayesian InferenceCryptic SpeciesD2-D3Pcr-Its-RflpRdnaSheath Nematodeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The genus Hemicycliophora (Nematoda: Hemicycliophoridae) contains 132 valid species of plant-parasitic nematodes, collectively known as ‘sheath nematodes’. Hemicycliophora spp. are characterized morphologically by a long stylet with rounded basal knobs and a cuticular sheath, present in juvenile and adult stages. Populations of 20 valid and 14 putative species of Hemicycliophora and Loofia from several countries were characterized morphologically using light (LM) and scanning electron microscopy (SEM) and molecularly using the D2-D3 segments of 28S rRNA and internal transcribed spacer (ITS) rRNA gene sequences. LM and SEM observations provided new details on the morphology of these species. PCR-restriction fragment length polymorphisms (PCR-RFLPs) of the D2-D3 of 28S rDNA were proposed for identification of the species. Phylogenetic relationships within populations of 36 species of the genus Hemicycliophora using 102 D2-D3 of 28S rDNA and 97 ITS rRNA gene sequences as inferred from Bayesian analysis are reconstructed and discussed. Ancestral state reconstructions of diagnostic characters (body and stylet length, number of body annuli, shape of vulval lip and tail), using maximum parsimony and Bayesian inference, revealed that none of the traits are individually reliable characters for classifying the studied sheath nematode. The Shimodaira–Hasegawa test rejected the validity of the genus Loofia. This is the most complete phylogenetic analysis of Hemicycliophora species conducted so far.Fil: Subbotin, Sergei A.. California Department of Food and Agriculture; Estados Unidos. Institute of Ecology and Evolution of the Russian Academy of Sciences; RusiaFil: Chitambar, John J.. California Department of Food and Agriculture; Estados UnidosFil: Chizhov, Vladimir N.. Institute of Ecology and Evolution of the Russian Academy of Sciences; RusiaFil: Stanley, Jason D.. Florida Department of Agriculture and Consumer Services; Estados UnidosFil: Inserra, Renato N.. Florida Department of Agriculture and Consumer Services; Estados UnidosFil: Doucet, Marcelo Edmundo. Universidad Nacional de Cordoba. Facultad de Cs.exactas Fisicas y Naturales. Centro de Zoologia Aplicada; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; ArgentinaFil: Mcclure, Michael. University Of Arizona; Estados UnidosFil: Ye, Weimin. North Carolina Department of Agriculture & Consumer Services; Estados UnidosFil: Yeates, George.Fil: Mollov, Dimitre S.. University Of Minnesota; Estados UnidosFil: Cantalapiedra Navarrete, Carolina. Consejo Superior de Investigaciones Científicas. Instituto de Agricultura Sostenible; EspañaFil: Vovlas, Nicola. Istituto per la Protezione delle Piante; ItaliaFil: Van Den Berg, Esther. ARC-Plant Protection Research Institute; SudáfricaFil: Castillo, Pablo. Consejo Superior de Investigaciones Científicas. Instituto de Agricultura Sostenible; EspañaWiley2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8002Subbotin, Sergei A.; Chitambar, John J.; Chizhov, Vladimir N.; Stanley, Jason D.; Inserra, Renato N.; et al.; Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae); Wiley; Zoological Journal Of The Linnean Society; 171; 3; 10-2014; 475-5060024-4082enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/zoj.12145/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/zoj.12145info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:31Zoai:ri.conicet.gov.ar:11336/8002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:31.89CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)
title Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)
spellingShingle Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)
Subbotin, Sergei A.
Ancestral State Reconstructions
Bayesian Inference
Cryptic Species
D2-D3
Pcr-Its-Rflp
Rdna
Sheath Nematodes
title_short Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)
title_full Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)
title_fullStr Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)
title_full_unstemmed Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)
title_sort Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)
dc.creator.none.fl_str_mv Subbotin, Sergei A.
Chitambar, John J.
Chizhov, Vladimir N.
Stanley, Jason D.
Inserra, Renato N.
Doucet, Marcelo Edmundo
Mcclure, Michael
Ye, Weimin
Yeates, George
Mollov, Dimitre S.
Cantalapiedra Navarrete, Carolina
Vovlas, Nicola
Van Den Berg, Esther
Castillo, Pablo
author Subbotin, Sergei A.
author_facet Subbotin, Sergei A.
Chitambar, John J.
Chizhov, Vladimir N.
Stanley, Jason D.
Inserra, Renato N.
Doucet, Marcelo Edmundo
Mcclure, Michael
Ye, Weimin
Yeates, George
Mollov, Dimitre S.
Cantalapiedra Navarrete, Carolina
Vovlas, Nicola
Van Den Berg, Esther
Castillo, Pablo
author_role author
author2 Chitambar, John J.
Chizhov, Vladimir N.
Stanley, Jason D.
Inserra, Renato N.
Doucet, Marcelo Edmundo
Mcclure, Michael
Ye, Weimin
Yeates, George
Mollov, Dimitre S.
Cantalapiedra Navarrete, Carolina
Vovlas, Nicola
Van Den Berg, Esther
Castillo, Pablo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ancestral State Reconstructions
Bayesian Inference
Cryptic Species
D2-D3
Pcr-Its-Rflp
Rdna
Sheath Nematodes
topic Ancestral State Reconstructions
Bayesian Inference
Cryptic Species
D2-D3
Pcr-Its-Rflp
Rdna
Sheath Nematodes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The genus Hemicycliophora (Nematoda: Hemicycliophoridae) contains 132 valid species of plant-parasitic nematodes, collectively known as ‘sheath nematodes’. Hemicycliophora spp. are characterized morphologically by a long stylet with rounded basal knobs and a cuticular sheath, present in juvenile and adult stages. Populations of 20 valid and 14 putative species of Hemicycliophora and Loofia from several countries were characterized morphologically using light (LM) and scanning electron microscopy (SEM) and molecularly using the D2-D3 segments of 28S rRNA and internal transcribed spacer (ITS) rRNA gene sequences. LM and SEM observations provided new details on the morphology of these species. PCR-restriction fragment length polymorphisms (PCR-RFLPs) of the D2-D3 of 28S rDNA were proposed for identification of the species. Phylogenetic relationships within populations of 36 species of the genus Hemicycliophora using 102 D2-D3 of 28S rDNA and 97 ITS rRNA gene sequences as inferred from Bayesian analysis are reconstructed and discussed. Ancestral state reconstructions of diagnostic characters (body and stylet length, number of body annuli, shape of vulval lip and tail), using maximum parsimony and Bayesian inference, revealed that none of the traits are individually reliable characters for classifying the studied sheath nematode. The Shimodaira–Hasegawa test rejected the validity of the genus Loofia. This is the most complete phylogenetic analysis of Hemicycliophora species conducted so far.
Fil: Subbotin, Sergei A.. California Department of Food and Agriculture; Estados Unidos. Institute of Ecology and Evolution of the Russian Academy of Sciences; Rusia
Fil: Chitambar, John J.. California Department of Food and Agriculture; Estados Unidos
Fil: Chizhov, Vladimir N.. Institute of Ecology and Evolution of the Russian Academy of Sciences; Rusia
Fil: Stanley, Jason D.. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Inserra, Renato N.. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Doucet, Marcelo Edmundo. Universidad Nacional de Cordoba. Facultad de Cs.exactas Fisicas y Naturales. Centro de Zoologia Aplicada; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; Argentina
Fil: Mcclure, Michael. University Of Arizona; Estados Unidos
Fil: Ye, Weimin. North Carolina Department of Agriculture & Consumer Services; Estados Unidos
Fil: Yeates, George.
Fil: Mollov, Dimitre S.. University Of Minnesota; Estados Unidos
Fil: Cantalapiedra Navarrete, Carolina. Consejo Superior de Investigaciones Científicas. Instituto de Agricultura Sostenible; España
Fil: Vovlas, Nicola. Istituto per la Protezione delle Piante; Italia
Fil: Van Den Berg, Esther. ARC-Plant Protection Research Institute; Sudáfrica
Fil: Castillo, Pablo. Consejo Superior de Investigaciones Científicas. Instituto de Agricultura Sostenible; España
description The genus Hemicycliophora (Nematoda: Hemicycliophoridae) contains 132 valid species of plant-parasitic nematodes, collectively known as ‘sheath nematodes’. Hemicycliophora spp. are characterized morphologically by a long stylet with rounded basal knobs and a cuticular sheath, present in juvenile and adult stages. Populations of 20 valid and 14 putative species of Hemicycliophora and Loofia from several countries were characterized morphologically using light (LM) and scanning electron microscopy (SEM) and molecularly using the D2-D3 segments of 28S rRNA and internal transcribed spacer (ITS) rRNA gene sequences. LM and SEM observations provided new details on the morphology of these species. PCR-restriction fragment length polymorphisms (PCR-RFLPs) of the D2-D3 of 28S rDNA were proposed for identification of the species. Phylogenetic relationships within populations of 36 species of the genus Hemicycliophora using 102 D2-D3 of 28S rDNA and 97 ITS rRNA gene sequences as inferred from Bayesian analysis are reconstructed and discussed. Ancestral state reconstructions of diagnostic characters (body and stylet length, number of body annuli, shape of vulval lip and tail), using maximum parsimony and Bayesian inference, revealed that none of the traits are individually reliable characters for classifying the studied sheath nematode. The Shimodaira–Hasegawa test rejected the validity of the genus Loofia. This is the most complete phylogenetic analysis of Hemicycliophora species conducted so far.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8002
Subbotin, Sergei A.; Chitambar, John J.; Chizhov, Vladimir N.; Stanley, Jason D.; Inserra, Renato N.; et al.; Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae); Wiley; Zoological Journal Of The Linnean Society; 171; 3; 10-2014; 475-506
0024-4082
url http://hdl.handle.net/11336/8002
identifier_str_mv Subbotin, Sergei A.; Chitambar, John J.; Chizhov, Vladimir N.; Stanley, Jason D.; Inserra, Renato N.; et al.; Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae); Wiley; Zoological Journal Of The Linnean Society; 171; 3; 10-2014; 475-506
0024-4082
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/zoj.12145/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/zoj.12145
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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