Quantum dot-modified paper-based assay for glucose screening

Autores
Durán, Gema M.; Benavidez, Tomás Enrique; Ríos, Ángel; Garcia, Carlos D.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The article describes a simple optical assay for glucose. It is based on the use of paper spots loaded with colloidal CdSe/ZnS quantum dots (Q-dots) and the enzyme glucose oxidase (GOx). Circular paper sheets were uniformly loaded with Q-dots and then displayed strong fluorescence under a UV lamp (365 nm exCitation). The action of GOx causes the production of H2O2 which, after a typical exposure time of 20 min, causes fluorescence intensity to be quenched. To obtain a reading, the paper sheets were photographed under 365 nm excitation using a digital camera. Several parameters, including the amount of Q-dots, sample pH, and amount of GOx were optimized to maximize the response to glucose. The paper-based assay showed a sigmoidal-shaped response with respect to the glucose concentration in the 5–200 mg·dL−1 range (limit of detection of 5 μg·dL−1), demonstrating their potential use for biomedical applications.
Fil: Durán, Gema M.. Universidad de Castilla-La Mancha; España. Instituto Regional de Investigación Científica Aplicada; España
Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Clemson University; Estados Unidos
Fil: Ríos, Ángel. Universidad de Castilla-La Mancha; España
Fil: Garcia, Carlos D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Clemson University; Estados Unidos
Materia
GLUCOSE
PAPER-BASED ASSAY
QUANTUM DOTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54967
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54967
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quantum dot-modified paper-based assay for glucose screeningDurán, Gema M.Benavidez, Tomás EnriqueRíos, ÁngelGarcia, Carlos D.GLUCOSEPAPER-BASED ASSAYQUANTUM DOTShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The article describes a simple optical assay for glucose. It is based on the use of paper spots loaded with colloidal CdSe/ZnS quantum dots (Q-dots) and the enzyme glucose oxidase (GOx). Circular paper sheets were uniformly loaded with Q-dots and then displayed strong fluorescence under a UV lamp (365 nm exCitation). The action of GOx causes the production of H2O2 which, after a typical exposure time of 20 min, causes fluorescence intensity to be quenched. To obtain a reading, the paper sheets were photographed under 365 nm excitation using a digital camera. Several parameters, including the amount of Q-dots, sample pH, and amount of GOx were optimized to maximize the response to glucose. The paper-based assay showed a sigmoidal-shaped response with respect to the glucose concentration in the 5–200 mg·dL−1 range (limit of detection of 5 μg·dL−1), demonstrating their potential use for biomedical applications.Fil: Durán, Gema M.. Universidad de Castilla-La Mancha; España. Instituto Regional de Investigación Científica Aplicada; EspañaFil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Clemson University; Estados UnidosFil: Ríos, Ángel. Universidad de Castilla-La Mancha; EspañaFil: Garcia, Carlos D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Clemson University; Estados UnidosSpringer Wien2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54967Durán, Gema M.; Benavidez, Tomás Enrique; Ríos, Ángel; Garcia, Carlos D.; Quantum dot-modified paper-based assay for glucose screening; Springer Wien; Mikrochimica Acta; 183; 2; 2-2016; 611-6160026-36721436-5073CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1007/s00604-015-1685-3info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820292/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T09:38:59Zoai:ri.conicet.gov.ar:11336/54967instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 09:38:59.807CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantum dot-modified paper-based assay for glucose screening
title Quantum dot-modified paper-based assay for glucose screening
spellingShingle Quantum dot-modified paper-based assay for glucose screening
Durán, Gema M.
GLUCOSE
PAPER-BASED ASSAY
QUANTUM DOTS
title_short Quantum dot-modified paper-based assay for glucose screening
title_full Quantum dot-modified paper-based assay for glucose screening
title_fullStr Quantum dot-modified paper-based assay for glucose screening
title_full_unstemmed Quantum dot-modified paper-based assay for glucose screening
title_sort Quantum dot-modified paper-based assay for glucose screening
dc.creator.none.fl_str_mv Durán, Gema M.
Benavidez, Tomás Enrique
Ríos, Ángel
Garcia, Carlos D.
author Durán, Gema M.
author_facet Durán, Gema M.
Benavidez, Tomás Enrique
Ríos, Ángel
Garcia, Carlos D.
author_role author
author2 Benavidez, Tomás Enrique
Ríos, Ángel
Garcia, Carlos D.
author2_role author
author
author
dc.subject.none.fl_str_mv GLUCOSE
PAPER-BASED ASSAY
QUANTUM DOTS
topic GLUCOSE
PAPER-BASED ASSAY
QUANTUM DOTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The article describes a simple optical assay for glucose. It is based on the use of paper spots loaded with colloidal CdSe/ZnS quantum dots (Q-dots) and the enzyme glucose oxidase (GOx). Circular paper sheets were uniformly loaded with Q-dots and then displayed strong fluorescence under a UV lamp (365 nm exCitation). The action of GOx causes the production of H2O2 which, after a typical exposure time of 20 min, causes fluorescence intensity to be quenched. To obtain a reading, the paper sheets were photographed under 365 nm excitation using a digital camera. Several parameters, including the amount of Q-dots, sample pH, and amount of GOx were optimized to maximize the response to glucose. The paper-based assay showed a sigmoidal-shaped response with respect to the glucose concentration in the 5–200 mg·dL−1 range (limit of detection of 5 μg·dL−1), demonstrating their potential use for biomedical applications.
Fil: Durán, Gema M.. Universidad de Castilla-La Mancha; España. Instituto Regional de Investigación Científica Aplicada; España
Fil: Benavidez, Tomás Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Clemson University; Estados Unidos
Fil: Ríos, Ángel. Universidad de Castilla-La Mancha; España
Fil: Garcia, Carlos D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Clemson University; Estados Unidos
description The article describes a simple optical assay for glucose. It is based on the use of paper spots loaded with colloidal CdSe/ZnS quantum dots (Q-dots) and the enzyme glucose oxidase (GOx). Circular paper sheets were uniformly loaded with Q-dots and then displayed strong fluorescence under a UV lamp (365 nm exCitation). The action of GOx causes the production of H2O2 which, after a typical exposure time of 20 min, causes fluorescence intensity to be quenched. To obtain a reading, the paper sheets were photographed under 365 nm excitation using a digital camera. Several parameters, including the amount of Q-dots, sample pH, and amount of GOx were optimized to maximize the response to glucose. The paper-based assay showed a sigmoidal-shaped response with respect to the glucose concentration in the 5–200 mg·dL−1 range (limit of detection of 5 μg·dL−1), demonstrating their potential use for biomedical applications.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54967
Durán, Gema M.; Benavidez, Tomás Enrique; Ríos, Ángel; Garcia, Carlos D.; Quantum dot-modified paper-based assay for glucose screening; Springer Wien; Mikrochimica Acta; 183; 2; 2-2016; 611-616
0026-3672
1436-5073
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54967
identifier_str_mv Durán, Gema M.; Benavidez, Tomás Enrique; Ríos, Ángel; Garcia, Carlos D.; Quantum dot-modified paper-based assay for glucose screening; Springer Wien; Mikrochimica Acta; 183; 2; 2-2016; 611-616
0026-3672
1436-5073
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00604-015-1685-3
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4820292/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Wien
publisher.none.fl_str_mv Springer Wien
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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