Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer
- Autores
- Cerda Opazo, Paulina; Valenzuela Valderrama, Manuel; Wichmann, Ignacio; Rodríguez, Andrés; Contreras Reyes, Daniel; Fernandez, Elmer Andres; Carrasco Aviño, Gonzalo; Corvalan, Alejandro H.; Quest, Andrew F. G.
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- BACKGROUND: The objective of the study was to determine the relationship between Survivin and Reprimo transcript/protein expression levels, and gastric cancer outcome. METHODS: In silico correlations between an agnostic set of twelve p53-dependent apoptosis and cell-cycle genes were explored in the gastric adenocarcinoma TCGA database, using cBioPortal. Findings were validated by regression analysis of RNAseq data. Separate regression analyses were performed to assess the impact of p53 status on Survivin and Reprimo. Quantitative reverse-transcription PCR (RT-qPCR) and immunohistochemistry confirmed in silico findings on fresh-frozen and paraffin-embedded gastric cancer tissues, respectively. Wild-type (AGS, SNU-1) and mutated p53 (NCI-N87) cell lines transfected with pEGFP-Survivin or pCMV6-Reprimo were evaluated by RT-qPCR and Western blotting. Kaplan-Meier method and Long-Rank test were used to assess differences in patient outcome. RESULTS: cBioPortal analysis revealed an inverse correlation between Survivin and Reprimo expression (Pearson’s r= -0.3, Spearman’s ρ= -0.55). RNAseq analyses confirmed these findings (Spearman’s ρ= -0.37, p<4.2e-09) and revealed p53 dependence in linear regression models (p<0.05). mRNA and protein levels validated these observations in clinical samples (p<0.001). In vitro analysis in cell lines demonstrated that increasing Survivin reduced Reprimo, while increasing Reprimo reduced Survivin expression, but only did so in p53 wild-type gastric cells (p<0.05). Survivin-positive but Reprimo-negative patients displayed shorter overall survival rates (p=0.047, Long Rank Test) (HR=0.32; 95%IC: 0.11-0.97; p=0.044). CONCLUSIONS: TCGA RNAseq data analysis, evaluation of clinical samples and studies in cell lines identified an inverse relationship between Survivin and Reprimo. Elevated Survivin and reduced Reprimo protein expression correlated with poor patient prognosis in gastric cancer.
Fil: Cerda Opazo, Paulina. Universidad de Chile; Chile. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile
Fil: Valenzuela Valderrama, Manuel. Universidad de Chile; Chile. Universidad Central de Chile. Facultad de Ciencias de la Salud; Chile
Fil: Wichmann, Ignacio. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile
Fil: Rodríguez, Andrés. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile
Fil: Contreras Reyes, Daniel. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile
Fil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Plataforma Bioinformática Argentina; Argentina
Fil: Carrasco Aviño, Gonzalo. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile. Universidad de Chile; Chile
Fil: Corvalan, Alejandro H.. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile
Fil: Quest, Andrew F. G.. Universidad de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile - Materia
-
GASTRIC CANCER
REPRIMO
SURVIVIN
TCGA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/91295
Ver los metadatos del registro completo
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Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancerCerda Opazo, PaulinaValenzuela Valderrama, ManuelWichmann, IgnacioRodríguez, AndrésContreras Reyes, DanielFernandez, Elmer AndresCarrasco Aviño, GonzaloCorvalan, Alejandro H.Quest, Andrew F. G.GASTRIC CANCERREPRIMOSURVIVINTCGAhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3BACKGROUND: The objective of the study was to determine the relationship between Survivin and Reprimo transcript/protein expression levels, and gastric cancer outcome. METHODS: In silico correlations between an agnostic set of twelve p53-dependent apoptosis and cell-cycle genes were explored in the gastric adenocarcinoma TCGA database, using cBioPortal. Findings were validated by regression analysis of RNAseq data. Separate regression analyses were performed to assess the impact of p53 status on Survivin and Reprimo. Quantitative reverse-transcription PCR (RT-qPCR) and immunohistochemistry confirmed in silico findings on fresh-frozen and paraffin-embedded gastric cancer tissues, respectively. Wild-type (AGS, SNU-1) and mutated p53 (NCI-N87) cell lines transfected with pEGFP-Survivin or pCMV6-Reprimo were evaluated by RT-qPCR and Western blotting. Kaplan-Meier method and Long-Rank test were used to assess differences in patient outcome. RESULTS: cBioPortal analysis revealed an inverse correlation between Survivin and Reprimo expression (Pearson’s r= -0.3, Spearman’s ρ= -0.55). RNAseq analyses confirmed these findings (Spearman’s ρ= -0.37, p<4.2e-09) and revealed p53 dependence in linear regression models (p<0.05). mRNA and protein levels validated these observations in clinical samples (p<0.001). In vitro analysis in cell lines demonstrated that increasing Survivin reduced Reprimo, while increasing Reprimo reduced Survivin expression, but only did so in p53 wild-type gastric cells (p<0.05). Survivin-positive but Reprimo-negative patients displayed shorter overall survival rates (p=0.047, Long Rank Test) (HR=0.32; 95%IC: 0.11-0.97; p=0.044). CONCLUSIONS: TCGA RNAseq data analysis, evaluation of clinical samples and studies in cell lines identified an inverse relationship between Survivin and Reprimo. Elevated Survivin and reduced Reprimo protein expression correlated with poor patient prognosis in gastric cancer.Fil: Cerda Opazo, Paulina. Universidad de Chile; Chile. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; ChileFil: Valenzuela Valderrama, Manuel. Universidad de Chile; Chile. Universidad Central de Chile. Facultad de Ciencias de la Salud; ChileFil: Wichmann, Ignacio. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; ChileFil: Rodríguez, Andrés. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; ChileFil: Contreras Reyes, Daniel. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; ChileFil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Plataforma Bioinformática Argentina; ArgentinaFil: Carrasco Aviño, Gonzalo. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile. Universidad de Chile; ChileFil: Corvalan, Alejandro H.. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; ChileFil: Quest, Andrew F. G.. Universidad de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; ChileImpact Journals2018-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91295Cerda Opazo, Paulina; Valenzuela Valderrama, Manuel; Wichmann, Ignacio; Rodríguez, Andrés; Contreras Reyes, Daniel; et al.; Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer; Impact Journals; Oncotarget; 9; 16; 2-2018; 12853-128671949-2553CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.24402info:eu-repo/semantics/altIdentifier/url/http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=24402&path[]=76641info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:04Zoai:ri.conicet.gov.ar:11336/91295instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:04.553CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer |
title |
Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer |
spellingShingle |
Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer Cerda Opazo, Paulina GASTRIC CANCER REPRIMO SURVIVIN TCGA |
title_short |
Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer |
title_full |
Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer |
title_fullStr |
Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer |
title_full_unstemmed |
Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer |
title_sort |
Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer |
dc.creator.none.fl_str_mv |
Cerda Opazo, Paulina Valenzuela Valderrama, Manuel Wichmann, Ignacio Rodríguez, Andrés Contreras Reyes, Daniel Fernandez, Elmer Andres Carrasco Aviño, Gonzalo Corvalan, Alejandro H. Quest, Andrew F. G. |
author |
Cerda Opazo, Paulina |
author_facet |
Cerda Opazo, Paulina Valenzuela Valderrama, Manuel Wichmann, Ignacio Rodríguez, Andrés Contreras Reyes, Daniel Fernandez, Elmer Andres Carrasco Aviño, Gonzalo Corvalan, Alejandro H. Quest, Andrew F. G. |
author_role |
author |
author2 |
Valenzuela Valderrama, Manuel Wichmann, Ignacio Rodríguez, Andrés Contreras Reyes, Daniel Fernandez, Elmer Andres Carrasco Aviño, Gonzalo Corvalan, Alejandro H. Quest, Andrew F. G. |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
GASTRIC CANCER REPRIMO SURVIVIN TCGA |
topic |
GASTRIC CANCER REPRIMO SURVIVIN TCGA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
BACKGROUND: The objective of the study was to determine the relationship between Survivin and Reprimo transcript/protein expression levels, and gastric cancer outcome. METHODS: In silico correlations between an agnostic set of twelve p53-dependent apoptosis and cell-cycle genes were explored in the gastric adenocarcinoma TCGA database, using cBioPortal. Findings were validated by regression analysis of RNAseq data. Separate regression analyses were performed to assess the impact of p53 status on Survivin and Reprimo. Quantitative reverse-transcription PCR (RT-qPCR) and immunohistochemistry confirmed in silico findings on fresh-frozen and paraffin-embedded gastric cancer tissues, respectively. Wild-type (AGS, SNU-1) and mutated p53 (NCI-N87) cell lines transfected with pEGFP-Survivin or pCMV6-Reprimo were evaluated by RT-qPCR and Western blotting. Kaplan-Meier method and Long-Rank test were used to assess differences in patient outcome. RESULTS: cBioPortal analysis revealed an inverse correlation between Survivin and Reprimo expression (Pearson’s r= -0.3, Spearman’s ρ= -0.55). RNAseq analyses confirmed these findings (Spearman’s ρ= -0.37, p<4.2e-09) and revealed p53 dependence in linear regression models (p<0.05). mRNA and protein levels validated these observations in clinical samples (p<0.001). In vitro analysis in cell lines demonstrated that increasing Survivin reduced Reprimo, while increasing Reprimo reduced Survivin expression, but only did so in p53 wild-type gastric cells (p<0.05). Survivin-positive but Reprimo-negative patients displayed shorter overall survival rates (p=0.047, Long Rank Test) (HR=0.32; 95%IC: 0.11-0.97; p=0.044). CONCLUSIONS: TCGA RNAseq data analysis, evaluation of clinical samples and studies in cell lines identified an inverse relationship between Survivin and Reprimo. Elevated Survivin and reduced Reprimo protein expression correlated with poor patient prognosis in gastric cancer. Fil: Cerda Opazo, Paulina. Universidad de Chile; Chile. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile Fil: Valenzuela Valderrama, Manuel. Universidad de Chile; Chile. Universidad Central de Chile. Facultad de Ciencias de la Salud; Chile Fil: Wichmann, Ignacio. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile Fil: Rodríguez, Andrés. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile Fil: Contreras Reyes, Daniel. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile Fil: Fernandez, Elmer Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas. Universidad Católica de Córdoba. Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina. Plataforma Bioinformática Argentina; Argentina Fil: Carrasco Aviño, Gonzalo. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile. Universidad de Chile; Chile Fil: Corvalan, Alejandro H.. Pontificia Universidad Católica de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile Fil: Quest, Andrew F. G.. Universidad de Chile; Chile. Centro de Estudios Avanzados de Enfermedades Crónicas; Chile |
description |
BACKGROUND: The objective of the study was to determine the relationship between Survivin and Reprimo transcript/protein expression levels, and gastric cancer outcome. METHODS: In silico correlations between an agnostic set of twelve p53-dependent apoptosis and cell-cycle genes were explored in the gastric adenocarcinoma TCGA database, using cBioPortal. Findings were validated by regression analysis of RNAseq data. Separate regression analyses were performed to assess the impact of p53 status on Survivin and Reprimo. Quantitative reverse-transcription PCR (RT-qPCR) and immunohistochemistry confirmed in silico findings on fresh-frozen and paraffin-embedded gastric cancer tissues, respectively. Wild-type (AGS, SNU-1) and mutated p53 (NCI-N87) cell lines transfected with pEGFP-Survivin or pCMV6-Reprimo were evaluated by RT-qPCR and Western blotting. Kaplan-Meier method and Long-Rank test were used to assess differences in patient outcome. RESULTS: cBioPortal analysis revealed an inverse correlation between Survivin and Reprimo expression (Pearson’s r= -0.3, Spearman’s ρ= -0.55). RNAseq analyses confirmed these findings (Spearman’s ρ= -0.37, p<4.2e-09) and revealed p53 dependence in linear regression models (p<0.05). mRNA and protein levels validated these observations in clinical samples (p<0.001). In vitro analysis in cell lines demonstrated that increasing Survivin reduced Reprimo, while increasing Reprimo reduced Survivin expression, but only did so in p53 wild-type gastric cells (p<0.05). Survivin-positive but Reprimo-negative patients displayed shorter overall survival rates (p=0.047, Long Rank Test) (HR=0.32; 95%IC: 0.11-0.97; p=0.044). CONCLUSIONS: TCGA RNAseq data analysis, evaluation of clinical samples and studies in cell lines identified an inverse relationship between Survivin and Reprimo. Elevated Survivin and reduced Reprimo protein expression correlated with poor patient prognosis in gastric cancer. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/91295 Cerda Opazo, Paulina; Valenzuela Valderrama, Manuel; Wichmann, Ignacio; Rodríguez, Andrés; Contreras Reyes, Daniel; et al.; Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer; Impact Journals; Oncotarget; 9; 16; 2-2018; 12853-12867 1949-2553 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/91295 |
identifier_str_mv |
Cerda Opazo, Paulina; Valenzuela Valderrama, Manuel; Wichmann, Ignacio; Rodríguez, Andrés; Contreras Reyes, Daniel; et al.; Inverse expression of survivin and reprimo correlates with poor patient prognosis in gastric cancer; Impact Journals; Oncotarget; 9; 16; 2-2018; 12853-12867 1949-2553 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.24402 info:eu-repo/semantics/altIdentifier/url/http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=24402&path[]=76641 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Impact Journals |
publisher.none.fl_str_mv |
Impact Journals |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613800003633152 |
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13.070432 |