Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease

Autores
Maharramov, Jafar; Meeus, Ivan; Maebe, Kevin; Arbetman, Marina Paula; Morales, Carolina Laura; Graystock, Peter; Hughes, William O. H.; Plischuk, Santiago; Lange, Carlos Ernesto; de Graaf, Dirk C.; Zapata, Nelson; Perez de la Rosa, José Javier; Murray, Tomás E.; Brown, Mark J. F.; Smagghe, Guy
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The worldwide spread of diseases is considered a major threat to biodiversity and a possible driver of the decline ofpollinator populations, particularly when novel species or strains of parasites emerge. Previous studies have suggested thatpopulations of introduced European honeybee (Apis mellifera) and bumblebee species (Bombus terrestris and Bombusruderatus) in Argentina share the neogregarine parasite Apicystis bombi with the native bumblebee (Bombus dahlbomii). Inthis study we investigated whether A. bombi is acting as an emergent parasite in the non-native populations. Specifically,we asked whether A. bombi, recently identified in Argentina, was introduced by European, non-native bees. Using ITS1 andITS2 to assess the parasite?s intraspecific genetic variation in bees from Argentina and Europe, we found a largelyunstructured parasite population, with only 15% of the genetic variation being explained by geographic location. The mostabundant haplotype in Argentina (found in all 9 specimens of non-native species) was identical to the most abundanthaplotype in Europe (found in 6 out of 8 specimens). Similarly, there was no evidence of structuring by host species, withthis factor explaining only 17% of the genetic variation. Interestingly, parasites in native Bombus ephippiatus from Mexicowere genetically distant from the Argentine and European samples, suggesting that sufficient variability does exist in the ITSregion to identify continent-level genetic structure in the parasite. Thus, the data suggest that A. bombi from Argentina andEurope share a common, relatively recent origin. Although our data did not provide information on the direction of transfer,the absence of genetic structure across space and host species suggests that A. bombi may be acting as an emergentinfectious disease across bee taxa and continents.
Fil: Maharramov, Jafar. University of Ghent; Bélgica
Fil: Meeus, Ivan. University of Ghent; Bélgica
Fil: Maebe, Kevin. University of Ghent; Bélgica
Fil: Arbetman, Marina Paula. Universidad Nacional de Río Negro. Facultad de Agronomía. Departamento de Ciencias Exactas, Naturales y de Ingeniería; Argentina. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Laboratorio de Ecotono; Argentina
Fil: Morales, Carolina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Laboratorio de Ecotono; Argentina
Fil: Graystock, Peter. University of Leeds; Reino Unido
Fil: Hughes, William O. H.. University of Sussex; Reino Unido
Fil: Plischuk, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: Lange, Carlos Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: de Graaf, Dirk C.. University of Ghent; Bélgica
Fil: Zapata, Nelson. Universidad de Concepción; Chile
Fil: Perez de la Rosa, José Javier. No especifíca;
Fil: Murray, Tomás E.. No especifíca;
Fil: Brown, Mark J. F.. No especifíca;
Fil: Smagghe, Guy. University of Ghent; Bélgica
Materia
Apicystis Bombi
Apis Mellifera
Invasion
Pathogen Spillover
Bombus Terrestris
Bombus Ruderatus
Multiple-Host Parasite
Europe
Argentina
Its-Region 1 And 2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2557
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2557
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee DiseaseMaharramov, JafarMeeus, IvanMaebe, KevinArbetman, Marina PaulaMorales, Carolina LauraGraystock, PeterHughes, William O. H.Plischuk, SantiagoLange, Carlos Ernestode Graaf, Dirk C.Zapata, NelsonPerez de la Rosa, José JavierMurray, Tomás E.Brown, Mark J. F.Smagghe, GuyApicystis BombiApis MelliferaInvasionPathogen SpilloverBombus TerrestrisBombus RuderatusMultiple-Host ParasiteEuropeArgentinaIts-Region 1 And 2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The worldwide spread of diseases is considered a major threat to biodiversity and a possible driver of the decline ofpollinator populations, particularly when novel species or strains of parasites emerge. Previous studies have suggested thatpopulations of introduced European honeybee (Apis mellifera) and bumblebee species (Bombus terrestris and Bombusruderatus) in Argentina share the neogregarine parasite Apicystis bombi with the native bumblebee (Bombus dahlbomii). Inthis study we investigated whether A. bombi is acting as an emergent parasite in the non-native populations. Specifically,we asked whether A. bombi, recently identified in Argentina, was introduced by European, non-native bees. Using ITS1 andITS2 to assess the parasite?s intraspecific genetic variation in bees from Argentina and Europe, we found a largelyunstructured parasite population, with only 15% of the genetic variation being explained by geographic location. The mostabundant haplotype in Argentina (found in all 9 specimens of non-native species) was identical to the most abundanthaplotype in Europe (found in 6 out of 8 specimens). Similarly, there was no evidence of structuring by host species, withthis factor explaining only 17% of the genetic variation. Interestingly, parasites in native Bombus ephippiatus from Mexicowere genetically distant from the Argentine and European samples, suggesting that sufficient variability does exist in the ITSregion to identify continent-level genetic structure in the parasite. Thus, the data suggest that A. bombi from Argentina andEurope share a common, relatively recent origin. Although our data did not provide information on the direction of transfer,the absence of genetic structure across space and host species suggests that A. bombi may be acting as an emergentinfectious disease across bee taxa and continents.Fil: Maharramov, Jafar. University of Ghent; BélgicaFil: Meeus, Ivan. University of Ghent; BélgicaFil: Maebe, Kevin. University of Ghent; BélgicaFil: Arbetman, Marina Paula. Universidad Nacional de Río Negro. Facultad de Agronomía. Departamento de Ciencias Exactas, Naturales y de Ingeniería; Argentina. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Laboratorio de Ecotono; ArgentinaFil: Morales, Carolina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Laboratorio de Ecotono; ArgentinaFil: Graystock, Peter. University of Leeds; Reino UnidoFil: Hughes, William O. H.. University of Sussex; Reino UnidoFil: Plischuk, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); ArgentinaFil: Lange, Carlos Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); ArgentinaFil: de Graaf, Dirk C.. University of Ghent; BélgicaFil: Zapata, Nelson. Universidad de Concepción; ChileFil: Perez de la Rosa, José Javier. No especifíca;Fil: Murray, Tomás E.. No especifíca;Fil: Brown, Mark J. F.. No especifíca;Fil: Smagghe, Guy. University of Ghent; BélgicaPublic Library of Science2013-12-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2557Maharramov, Jafar; Meeus, Ivan; Maebe, Kevin; Arbetman, Marina Paula; Morales, Carolina Laura; et al.; Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease; Public Library of Science; Plos One; 8; 12; 6-12-2013; 1-81932-6203enginfo:eu-repo/semantics/altIdentifier/url/www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0081475info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3855659/info:eu-repo/semantics/altIdentifier/doi/10.1371%2Fjournal.pone.0081475info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:23:40Zoai:ri.conicet.gov.ar:11336/2557instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:23:40.52CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease
title Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease
spellingShingle Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease
Maharramov, Jafar
Apicystis Bombi
Apis Mellifera
Invasion
Pathogen Spillover
Bombus Terrestris
Bombus Ruderatus
Multiple-Host Parasite
Europe
Argentina
Its-Region 1 And 2
title_short Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease
title_full Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease
title_fullStr Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease
title_full_unstemmed Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease
title_sort Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease
dc.creator.none.fl_str_mv Maharramov, Jafar
Meeus, Ivan
Maebe, Kevin
Arbetman, Marina Paula
Morales, Carolina Laura
Graystock, Peter
Hughes, William O. H.
Plischuk, Santiago
Lange, Carlos Ernesto
de Graaf, Dirk C.
Zapata, Nelson
Perez de la Rosa, José Javier
Murray, Tomás E.
Brown, Mark J. F.
Smagghe, Guy
author Maharramov, Jafar
author_facet Maharramov, Jafar
Meeus, Ivan
Maebe, Kevin
Arbetman, Marina Paula
Morales, Carolina Laura
Graystock, Peter
Hughes, William O. H.
Plischuk, Santiago
Lange, Carlos Ernesto
de Graaf, Dirk C.
Zapata, Nelson
Perez de la Rosa, José Javier
Murray, Tomás E.
Brown, Mark J. F.
Smagghe, Guy
author_role author
author2 Meeus, Ivan
Maebe, Kevin
Arbetman, Marina Paula
Morales, Carolina Laura
Graystock, Peter
Hughes, William O. H.
Plischuk, Santiago
Lange, Carlos Ernesto
de Graaf, Dirk C.
Zapata, Nelson
Perez de la Rosa, José Javier
Murray, Tomás E.
Brown, Mark J. F.
Smagghe, Guy
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Apicystis Bombi
Apis Mellifera
Invasion
Pathogen Spillover
Bombus Terrestris
Bombus Ruderatus
Multiple-Host Parasite
Europe
Argentina
Its-Region 1 And 2
topic Apicystis Bombi
Apis Mellifera
Invasion
Pathogen Spillover
Bombus Terrestris
Bombus Ruderatus
Multiple-Host Parasite
Europe
Argentina
Its-Region 1 And 2
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The worldwide spread of diseases is considered a major threat to biodiversity and a possible driver of the decline ofpollinator populations, particularly when novel species or strains of parasites emerge. Previous studies have suggested thatpopulations of introduced European honeybee (Apis mellifera) and bumblebee species (Bombus terrestris and Bombusruderatus) in Argentina share the neogregarine parasite Apicystis bombi with the native bumblebee (Bombus dahlbomii). Inthis study we investigated whether A. bombi is acting as an emergent parasite in the non-native populations. Specifically,we asked whether A. bombi, recently identified in Argentina, was introduced by European, non-native bees. Using ITS1 andITS2 to assess the parasite?s intraspecific genetic variation in bees from Argentina and Europe, we found a largelyunstructured parasite population, with only 15% of the genetic variation being explained by geographic location. The mostabundant haplotype in Argentina (found in all 9 specimens of non-native species) was identical to the most abundanthaplotype in Europe (found in 6 out of 8 specimens). Similarly, there was no evidence of structuring by host species, withthis factor explaining only 17% of the genetic variation. Interestingly, parasites in native Bombus ephippiatus from Mexicowere genetically distant from the Argentine and European samples, suggesting that sufficient variability does exist in the ITSregion to identify continent-level genetic structure in the parasite. Thus, the data suggest that A. bombi from Argentina andEurope share a common, relatively recent origin. Although our data did not provide information on the direction of transfer,the absence of genetic structure across space and host species suggests that A. bombi may be acting as an emergentinfectious disease across bee taxa and continents.
Fil: Maharramov, Jafar. University of Ghent; Bélgica
Fil: Meeus, Ivan. University of Ghent; Bélgica
Fil: Maebe, Kevin. University of Ghent; Bélgica
Fil: Arbetman, Marina Paula. Universidad Nacional de Río Negro. Facultad de Agronomía. Departamento de Ciencias Exactas, Naturales y de Ingeniería; Argentina. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Laboratorio de Ecotono; Argentina
Fil: Morales, Carolina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Laboratorio de Ecotono; Argentina
Fil: Graystock, Peter. University of Leeds; Reino Unido
Fil: Hughes, William O. H.. University of Sussex; Reino Unido
Fil: Plischuk, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: Lange, Carlos Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: de Graaf, Dirk C.. University of Ghent; Bélgica
Fil: Zapata, Nelson. Universidad de Concepción; Chile
Fil: Perez de la Rosa, José Javier. No especifíca;
Fil: Murray, Tomás E.. No especifíca;
Fil: Brown, Mark J. F.. No especifíca;
Fil: Smagghe, Guy. University of Ghent; Bélgica
description The worldwide spread of diseases is considered a major threat to biodiversity and a possible driver of the decline ofpollinator populations, particularly when novel species or strains of parasites emerge. Previous studies have suggested thatpopulations of introduced European honeybee (Apis mellifera) and bumblebee species (Bombus terrestris and Bombusruderatus) in Argentina share the neogregarine parasite Apicystis bombi with the native bumblebee (Bombus dahlbomii). Inthis study we investigated whether A. bombi is acting as an emergent parasite in the non-native populations. Specifically,we asked whether A. bombi, recently identified in Argentina, was introduced by European, non-native bees. Using ITS1 andITS2 to assess the parasite?s intraspecific genetic variation in bees from Argentina and Europe, we found a largelyunstructured parasite population, with only 15% of the genetic variation being explained by geographic location. The mostabundant haplotype in Argentina (found in all 9 specimens of non-native species) was identical to the most abundanthaplotype in Europe (found in 6 out of 8 specimens). Similarly, there was no evidence of structuring by host species, withthis factor explaining only 17% of the genetic variation. Interestingly, parasites in native Bombus ephippiatus from Mexicowere genetically distant from the Argentine and European samples, suggesting that sufficient variability does exist in the ITSregion to identify continent-level genetic structure in the parasite. Thus, the data suggest that A. bombi from Argentina andEurope share a common, relatively recent origin. Although our data did not provide information on the direction of transfer,the absence of genetic structure across space and host species suggests that A. bombi may be acting as an emergentinfectious disease across bee taxa and continents.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2557
Maharramov, Jafar; Meeus, Ivan; Maebe, Kevin; Arbetman, Marina Paula; Morales, Carolina Laura; et al.; Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease; Public Library of Science; Plos One; 8; 12; 6-12-2013; 1-8
1932-6203
url http://hdl.handle.net/11336/2557
identifier_str_mv Maharramov, Jafar; Meeus, Ivan; Maebe, Kevin; Arbetman, Marina Paula; Morales, Carolina Laura; et al.; Genetic Variability of the Neogregarine Apicystis bombi, an Etiological Agent of an Emergent Bumblebee Disease; Public Library of Science; Plos One; 8; 12; 6-12-2013; 1-8
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0081475
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3855659/
info:eu-repo/semantics/altIdentifier/doi/10.1371%2Fjournal.pone.0081475
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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