Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart

Autores
Prado, Natalia Jorgelina; Perdicaro, Diahann Jeanette; Parra, M.; Carrión, Adriana Miriam; Miatello, Roberto Miguel; Renna, Nicolas Federico; Ponce Zumino, Amira Zulma; Vazquez Prieto, M. A.; Diez, Emiliano Raúl
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oxidative stress during myocardial reperfusion contributes to ventricular arrhythmias onset. We aim to evaluate the antiarrhythmic effect of Malbec wine and resveratrol and compare them with the synthetic antioxidant tiron. Since alcohol use is controversial, we also assessed dealcoholized wine.Isolated hearts from male Sprague Dawley rats were perfused with Krebs-Henseleit solution added with: Malbec wine (5 ml/L), resveratrol (10 μM,) compared to controls with alcohol (0.5 ml/L); dealcoholized wine (5 ml/L), tiron (10 mM) were compared with controls without alcohol. Epicardial action potentials were analyzed during basal state, regional ischemia and reperfusion (10 minutes each period). The incidence of arrhythmias was determined. Te antioxidant effect was assessed in left ventricle homogenates and expressed as a percentage of inhibition of the ABTS?+ radical.Malbec wine and resveratrol reduced reperfusion arrhythmias in 56% and 50%, respectively, compared to 100% incidence in the control group with alcohol. Dealcoholized wine reduced arrhythmias to 50% compared to non-alcoholic control (90.5%), but tiron did not protect (69%). Te free radicals inhibitory effect increased with all the compounds (resveratrol 54.2%, tiron 43.2%, Malbec wine 42.9%, dealcoholized wine 40.2%) with respect to the control groups (with alcohol 23.5%, without alcohol 21.2%). Resveratrol shortened action potential duration and prevented ischemic depolarization. Malbec prevented ischemic-induced action potential shortening. We conclude that Malbec wine and resveratrol are antiarrhythmic beyond their antioxidant properties. Alcohol content or was not essential. Protection from ischemic action potential changes could be relevant to the antiarrhythmic effect of both resveratrol and wine.
Fil: Prado, Natalia Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Perdicaro DJ. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Parra, M. Universidad Nacional de Cuyo Facultad de Ciencias Médicas; Argentina
Fil: Carrión AM. Universidad Nacional de Cuyo Facultad de Ciencias Médicas; Argentina
Fil: Miatello RM. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Renna NF. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Ponce Zumino AZ. Universidad Nacional de Cuyo Facultad de Ciencias Médicas; Argentina
Fil: Vazquez Prieto M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Diez ER. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Materia
WINE
OXIDATIVE STRESS
RESVERATROL
ARRHYTHMIAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94102
Acceder
id CONICETDig_9cc4c9765652afcf34d18b42df61a74a
oai_identifier_str oai:ri.conicet.gov.ar:11336/94102
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heartPrado, Natalia JorgelinaPerdicaro, Diahann JeanetteParra, M.Carrión, Adriana MiriamMiatello, Roberto MiguelRenna, Nicolas FedericoPonce Zumino, Amira ZulmaVazquez Prieto, M. A.Diez, Emiliano RaúlWINEOXIDATIVE STRESSRESVERATROLARRHYTHMIAShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Oxidative stress during myocardial reperfusion contributes to ventricular arrhythmias onset. We aim to evaluate the antiarrhythmic effect of Malbec wine and resveratrol and compare them with the synthetic antioxidant tiron. Since alcohol use is controversial, we also assessed dealcoholized wine.Isolated hearts from male Sprague Dawley rats were perfused with Krebs-Henseleit solution added with: Malbec wine (5 ml/L), resveratrol (10 μM,) compared to controls with alcohol (0.5 ml/L); dealcoholized wine (5 ml/L), tiron (10 mM) were compared with controls without alcohol. Epicardial action potentials were analyzed during basal state, regional ischemia and reperfusion (10 minutes each period). The incidence of arrhythmias was determined. Te antioxidant effect was assessed in left ventricle homogenates and expressed as a percentage of inhibition of the ABTS?+ radical.Malbec wine and resveratrol reduced reperfusion arrhythmias in 56% and 50%, respectively, compared to 100% incidence in the control group with alcohol. Dealcoholized wine reduced arrhythmias to 50% compared to non-alcoholic control (90.5%), but tiron did not protect (69%). Te free radicals inhibitory effect increased with all the compounds (resveratrol 54.2%, tiron 43.2%, Malbec wine 42.9%, dealcoholized wine 40.2%) with respect to the control groups (with alcohol 23.5%, without alcohol 21.2%). Resveratrol shortened action potential duration and prevented ischemic depolarization. Malbec prevented ischemic-induced action potential shortening. We conclude that Malbec wine and resveratrol are antiarrhythmic beyond their antioxidant properties. Alcohol content or was not essential. Protection from ischemic action potential changes could be relevant to the antiarrhythmic effect of both resveratrol and wine.Fil: Prado, Natalia Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Perdicaro DJ. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Parra, M. Universidad Nacional de Cuyo Facultad de Ciencias Médicas; ArgentinaFil: Carrión AM. Universidad Nacional de Cuyo Facultad de Ciencias Médicas; ArgentinaFil: Miatello RM. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Renna NF. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Ponce Zumino AZ. Universidad Nacional de Cuyo Facultad de Ciencias Médicas; ArgentinaFil: Vazquez Prieto M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Diez ER. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaOpen Access Text2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94102Prado, Natalia Jorgelina; Perdicaro, Diahann Jeanette; Parra, M.; Carrión, Adriana Miriam; Miatello, Roberto Miguel; et al.; Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart; Open Access Text; Cardiovascular Disorders and Medicine; 3; 1; 2018; 1-72398-8878CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.15761/CDM.1000157info:eu-repo/semantics/altIdentifier/url/https://www.oatext.com/antiarrhythmic-mechanisms-of-malbec-wine-and-resveratrol-in-isolated-rat-heart.phpinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:13Zoai:ri.conicet.gov.ar:11336/94102instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:13.941CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart
title Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart
spellingShingle Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart
Prado, Natalia Jorgelina
WINE
OXIDATIVE STRESS
RESVERATROL
ARRHYTHMIAS
title_short Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart
title_full Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart
title_fullStr Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart
title_full_unstemmed Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart
title_sort Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart
dc.creator.none.fl_str_mv Prado, Natalia Jorgelina
Perdicaro, Diahann Jeanette
Parra, M.
Carrión, Adriana Miriam
Miatello, Roberto Miguel
Renna, Nicolas Federico
Ponce Zumino, Amira Zulma
Vazquez Prieto, M. A.
Diez, Emiliano Raúl
author Prado, Natalia Jorgelina
author_facet Prado, Natalia Jorgelina
Perdicaro, Diahann Jeanette
Parra, M.
Carrión, Adriana Miriam
Miatello, Roberto Miguel
Renna, Nicolas Federico
Ponce Zumino, Amira Zulma
Vazquez Prieto, M. A.
Diez, Emiliano Raúl
author_role author
author2 Perdicaro, Diahann Jeanette
Parra, M.
Carrión, Adriana Miriam
Miatello, Roberto Miguel
Renna, Nicolas Federico
Ponce Zumino, Amira Zulma
Vazquez Prieto, M. A.
Diez, Emiliano Raúl
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv WINE
OXIDATIVE STRESS
RESVERATROL
ARRHYTHMIAS
topic WINE
OXIDATIVE STRESS
RESVERATROL
ARRHYTHMIAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Oxidative stress during myocardial reperfusion contributes to ventricular arrhythmias onset. We aim to evaluate the antiarrhythmic effect of Malbec wine and resveratrol and compare them with the synthetic antioxidant tiron. Since alcohol use is controversial, we also assessed dealcoholized wine.Isolated hearts from male Sprague Dawley rats were perfused with Krebs-Henseleit solution added with: Malbec wine (5 ml/L), resveratrol (10 μM,) compared to controls with alcohol (0.5 ml/L); dealcoholized wine (5 ml/L), tiron (10 mM) were compared with controls without alcohol. Epicardial action potentials were analyzed during basal state, regional ischemia and reperfusion (10 minutes each period). The incidence of arrhythmias was determined. Te antioxidant effect was assessed in left ventricle homogenates and expressed as a percentage of inhibition of the ABTS?+ radical.Malbec wine and resveratrol reduced reperfusion arrhythmias in 56% and 50%, respectively, compared to 100% incidence in the control group with alcohol. Dealcoholized wine reduced arrhythmias to 50% compared to non-alcoholic control (90.5%), but tiron did not protect (69%). Te free radicals inhibitory effect increased with all the compounds (resveratrol 54.2%, tiron 43.2%, Malbec wine 42.9%, dealcoholized wine 40.2%) with respect to the control groups (with alcohol 23.5%, without alcohol 21.2%). Resveratrol shortened action potential duration and prevented ischemic depolarization. Malbec prevented ischemic-induced action potential shortening. We conclude that Malbec wine and resveratrol are antiarrhythmic beyond their antioxidant properties. Alcohol content or was not essential. Protection from ischemic action potential changes could be relevant to the antiarrhythmic effect of both resveratrol and wine.
Fil: Prado, Natalia Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Perdicaro DJ. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Parra, M. Universidad Nacional de Cuyo Facultad de Ciencias Médicas; Argentina
Fil: Carrión AM. Universidad Nacional de Cuyo Facultad de Ciencias Médicas; Argentina
Fil: Miatello RM. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Renna NF. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Ponce Zumino AZ. Universidad Nacional de Cuyo Facultad de Ciencias Médicas; Argentina
Fil: Vazquez Prieto M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Diez ER. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
description Oxidative stress during myocardial reperfusion contributes to ventricular arrhythmias onset. We aim to evaluate the antiarrhythmic effect of Malbec wine and resveratrol and compare them with the synthetic antioxidant tiron. Since alcohol use is controversial, we also assessed dealcoholized wine.Isolated hearts from male Sprague Dawley rats were perfused with Krebs-Henseleit solution added with: Malbec wine (5 ml/L), resveratrol (10 μM,) compared to controls with alcohol (0.5 ml/L); dealcoholized wine (5 ml/L), tiron (10 mM) were compared with controls without alcohol. Epicardial action potentials were analyzed during basal state, regional ischemia and reperfusion (10 minutes each period). The incidence of arrhythmias was determined. Te antioxidant effect was assessed in left ventricle homogenates and expressed as a percentage of inhibition of the ABTS?+ radical.Malbec wine and resveratrol reduced reperfusion arrhythmias in 56% and 50%, respectively, compared to 100% incidence in the control group with alcohol. Dealcoholized wine reduced arrhythmias to 50% compared to non-alcoholic control (90.5%), but tiron did not protect (69%). Te free radicals inhibitory effect increased with all the compounds (resveratrol 54.2%, tiron 43.2%, Malbec wine 42.9%, dealcoholized wine 40.2%) with respect to the control groups (with alcohol 23.5%, without alcohol 21.2%). Resveratrol shortened action potential duration and prevented ischemic depolarization. Malbec prevented ischemic-induced action potential shortening. We conclude that Malbec wine and resveratrol are antiarrhythmic beyond their antioxidant properties. Alcohol content or was not essential. Protection from ischemic action potential changes could be relevant to the antiarrhythmic effect of both resveratrol and wine.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94102
Prado, Natalia Jorgelina; Perdicaro, Diahann Jeanette; Parra, M.; Carrión, Adriana Miriam; Miatello, Roberto Miguel; et al.; Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart; Open Access Text; Cardiovascular Disorders and Medicine; 3; 1; 2018; 1-7
2398-8878
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94102
identifier_str_mv Prado, Natalia Jorgelina; Perdicaro, Diahann Jeanette; Parra, M.; Carrión, Adriana Miriam; Miatello, Roberto Miguel; et al.; Antiarrhythmic mechanisms of Malbec wine and resveratrol in isolated rat heart; Open Access Text; Cardiovascular Disorders and Medicine; 3; 1; 2018; 1-7
2398-8878
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.15761/CDM.1000157
info:eu-repo/semantics/altIdentifier/url/https://www.oatext.com/antiarrhythmic-mechanisms-of-malbec-wine-and-resveratrol-in-isolated-rat-heart.php
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Open Access Text
publisher.none.fl_str_mv Open Access Text
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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