Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae

Autores
Clementz, Adriana Laura; Aimaretti, Nora Rosa; Manuale, Débora Laura; Codevilla, Agustin; Yori, Juan Carlos
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Discarded carrots are a valuable source of biomass amenable for valorization. Their use as raw material for ethanol production by fermentation, using yeasts immobilized in Calcium alginate, was proposed. The biocatalyst immobilization method, the existence of internal and external mass transfer limitations, the effect of the initial pH and the reuse of immobilized yeasts were particularly evaluated. Results indicate that beads made with a 2 % solution of Sodium alginate and a 30 % solution of Saccharomyces cerevisiae were strong enough to allow an efficient nutrient transfer into the matrix and to prevent cell leaking. A stirring rate of 200 rpm was needed to avoid external mass transfer limitations. These beads were used in three successive fermentations. An initial pH of 5.5 reached the best fermentation parameters. Non-enriched, non-sterile carrot must was fermented through immobilized yeasts; and values of ethanol concentration (29.9 g L−1), Yp/s (0.409 g g−1), and productivity (7.45 g L−1 h−1) were obtained. These values were similar to those registered when free cells were used.
Fil: Clementz, Adriana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentina
Fil: Aimaretti, Nora Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentina. Universidad del Centro Educativo Latinoamericano; Argentina
Fil: Manuale, Débora Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentina
Fil: Codevilla, Agustin. Universidad del Centro Educativo Latinoamericano; Argentina
Fil: Yori, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentina
Materia
Bioethanol
Calcium Alginate Bead
Carrot Discard
Immobilization
Reuse
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37022
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/37022
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiaeClementz, Adriana LauraAimaretti, Nora RosaManuale, Débora LauraCodevilla, AgustinYori, Juan CarlosBioethanolCalcium Alginate BeadCarrot DiscardImmobilizationReusehttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Discarded carrots are a valuable source of biomass amenable for valorization. Their use as raw material for ethanol production by fermentation, using yeasts immobilized in Calcium alginate, was proposed. The biocatalyst immobilization method, the existence of internal and external mass transfer limitations, the effect of the initial pH and the reuse of immobilized yeasts were particularly evaluated. Results indicate that beads made with a 2 % solution of Sodium alginate and a 30 % solution of Saccharomyces cerevisiae were strong enough to allow an efficient nutrient transfer into the matrix and to prevent cell leaking. A stirring rate of 200 rpm was needed to avoid external mass transfer limitations. These beads were used in three successive fermentations. An initial pH of 5.5 reached the best fermentation parameters. Non-enriched, non-sterile carrot must was fermented through immobilized yeasts; and values of ethanol concentration (29.9 g L−1), Yp/s (0.409 g g−1), and productivity (7.45 g L−1 h−1) were obtained. These values were similar to those registered when free cells were used.Fil: Clementz, Adriana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; ArgentinaFil: Aimaretti, Nora Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentina. Universidad del Centro Educativo Latinoamericano; ArgentinaFil: Manuale, Débora Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; ArgentinaFil: Codevilla, Agustin. Universidad del Centro Educativo Latinoamericano; ArgentinaFil: Yori, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; ArgentinaSpringer Verlag2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37022Clementz, Adriana Laura; Aimaretti, Nora Rosa; Manuale, Débora Laura; Codevilla, Agustin; Yori, Juan Carlos; Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae; Springer Verlag; International Journal of Energy and Environmental Engineering; 6; 2; 6-2015; 129-1352008-91632251-6832CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs40095-014-0157-6info:eu-repo/semantics/altIdentifier/doi/10.1007/s40095-014-0157-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:31Zoai:ri.conicet.gov.ar:11336/37022instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:32.032CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae
title Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae
spellingShingle Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae
Clementz, Adriana Laura
Bioethanol
Calcium Alginate Bead
Carrot Discard
Immobilization
Reuse
title_short Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae
title_full Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae
title_fullStr Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae
title_full_unstemmed Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae
title_sort Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae
dc.creator.none.fl_str_mv Clementz, Adriana Laura
Aimaretti, Nora Rosa
Manuale, Débora Laura
Codevilla, Agustin
Yori, Juan Carlos
author Clementz, Adriana Laura
author_facet Clementz, Adriana Laura
Aimaretti, Nora Rosa
Manuale, Débora Laura
Codevilla, Agustin
Yori, Juan Carlos
author_role author
author2 Aimaretti, Nora Rosa
Manuale, Débora Laura
Codevilla, Agustin
Yori, Juan Carlos
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bioethanol
Calcium Alginate Bead
Carrot Discard
Immobilization
Reuse
topic Bioethanol
Calcium Alginate Bead
Carrot Discard
Immobilization
Reuse
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Discarded carrots are a valuable source of biomass amenable for valorization. Their use as raw material for ethanol production by fermentation, using yeasts immobilized in Calcium alginate, was proposed. The biocatalyst immobilization method, the existence of internal and external mass transfer limitations, the effect of the initial pH and the reuse of immobilized yeasts were particularly evaluated. Results indicate that beads made with a 2 % solution of Sodium alginate and a 30 % solution of Saccharomyces cerevisiae were strong enough to allow an efficient nutrient transfer into the matrix and to prevent cell leaking. A stirring rate of 200 rpm was needed to avoid external mass transfer limitations. These beads were used in three successive fermentations. An initial pH of 5.5 reached the best fermentation parameters. Non-enriched, non-sterile carrot must was fermented through immobilized yeasts; and values of ethanol concentration (29.9 g L−1), Yp/s (0.409 g g−1), and productivity (7.45 g L−1 h−1) were obtained. These values were similar to those registered when free cells were used.
Fil: Clementz, Adriana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentina
Fil: Aimaretti, Nora Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentina. Universidad del Centro Educativo Latinoamericano; Argentina
Fil: Manuale, Débora Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentina
Fil: Codevilla, Agustin. Universidad del Centro Educativo Latinoamericano; Argentina
Fil: Yori, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica ; Argentina
description Discarded carrots are a valuable source of biomass amenable for valorization. Their use as raw material for ethanol production by fermentation, using yeasts immobilized in Calcium alginate, was proposed. The biocatalyst immobilization method, the existence of internal and external mass transfer limitations, the effect of the initial pH and the reuse of immobilized yeasts were particularly evaluated. Results indicate that beads made with a 2 % solution of Sodium alginate and a 30 % solution of Saccharomyces cerevisiae were strong enough to allow an efficient nutrient transfer into the matrix and to prevent cell leaking. A stirring rate of 200 rpm was needed to avoid external mass transfer limitations. These beads were used in three successive fermentations. An initial pH of 5.5 reached the best fermentation parameters. Non-enriched, non-sterile carrot must was fermented through immobilized yeasts; and values of ethanol concentration (29.9 g L−1), Yp/s (0.409 g g−1), and productivity (7.45 g L−1 h−1) were obtained. These values were similar to those registered when free cells were used.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37022
Clementz, Adriana Laura; Aimaretti, Nora Rosa; Manuale, Débora Laura; Codevilla, Agustin; Yori, Juan Carlos; Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae; Springer Verlag; International Journal of Energy and Environmental Engineering; 6; 2; 6-2015; 129-135
2008-9163
2251-6832
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37022
identifier_str_mv Clementz, Adriana Laura; Aimaretti, Nora Rosa; Manuale, Débora Laura; Codevilla, Agustin; Yori, Juan Carlos; Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae; Springer Verlag; International Journal of Energy and Environmental Engineering; 6; 2; 6-2015; 129-135
2008-9163
2251-6832
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs40095-014-0157-6
info:eu-repo/semantics/altIdentifier/doi/10.1007/s40095-014-0157-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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