Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions

Autores
Bertellotti, Néstor Marcelo; Tella Escobedo, José Luis; Godoy, José A.; Blanco, Guillermo; Forero, Manuela G.; Donázar, José A.; Ceballos, Olga
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Magellanic Penguins (Spheniscus magellanicus) show little sexual dimorphism, and although males are usually larger than females, sexing by direct observation may be difficult, especially in the case of chicks. In this paper we evaluate the utility of four different PCR-based sex determination techniques using genomic DNA for sexing Magellanic Penguins. We found that the primer set designed for sex determination in Collared Flycatcher (Ficedula albicollis) also provided a reliable, simple and convenient sexing procedure for Magellanic Penguins. Additionally, we obtained discriminant functions for sexing adults and chicks, sampled at six colonies differing in size and other ecological characteristics. Discriminant function for adults used two variables, bill length and bill depth that correctly classified 97% of the birds. Discriminant function for chicks included bill length and flipper length and correctly classified 78% of the individuals. Although molecular and biometric approaches could be useful for sexing adult Magellanic Penguins, only molecular procedures proved appropriate for accurately sexing chicks.
Fil: Bertellotti, Néstor Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Tella Escobedo, José Luis. Consejo Superior de Investigaciones Científicas; España
Fil: Godoy, José A.. Consejo Superior de Investigaciones Científicas; España
Fil: Blanco, Guillermo. Universidad de Castilla-La Mancha. Instituto de Investigación en Recursos Cinegéticos; España. Consejo Superior de Investigaciones Científicas; España
Fil: Forero, Manuela G.. Consejo Superior de Investigaciones Científicas; España
Fil: Donázar, José A.. Consejo Superior de Investigaciones Científicas; España
Fil: Ceballos, Olga. Grupo de Estudios Biológicos Ugarra; España
Materia
DISCRIMINANT FUNCTIONS
SEXING BIRDS
MAGELLANIC PENGUIN
MOLECULAR SEXING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99128
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99128
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant FunctionsBertellotti, Néstor MarceloTella Escobedo, José LuisGodoy, José A.Blanco, GuillermoForero, Manuela G.Donázar, José A.Ceballos, OlgaDISCRIMINANT FUNCTIONSSEXING BIRDSMAGELLANIC PENGUINMOLECULAR SEXINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Magellanic Penguins (Spheniscus magellanicus) show little sexual dimorphism, and although males are usually larger than females, sexing by direct observation may be difficult, especially in the case of chicks. In this paper we evaluate the utility of four different PCR-based sex determination techniques using genomic DNA for sexing Magellanic Penguins. We found that the primer set designed for sex determination in Collared Flycatcher (Ficedula albicollis) also provided a reliable, simple and convenient sexing procedure for Magellanic Penguins. Additionally, we obtained discriminant functions for sexing adults and chicks, sampled at six colonies differing in size and other ecological characteristics. Discriminant function for adults used two variables, bill length and bill depth that correctly classified 97% of the birds. Discriminant function for chicks included bill length and flipper length and correctly classified 78% of the individuals. Although molecular and biometric approaches could be useful for sexing adult Magellanic Penguins, only molecular procedures proved appropriate for accurately sexing chicks.Fil: Bertellotti, Néstor Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Tella Escobedo, José Luis. Consejo Superior de Investigaciones Científicas; EspañaFil: Godoy, José A.. Consejo Superior de Investigaciones Científicas; EspañaFil: Blanco, Guillermo. Universidad de Castilla-La Mancha. Instituto de Investigación en Recursos Cinegéticos; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Forero, Manuela G.. Consejo Superior de Investigaciones Científicas; EspañaFil: Donázar, José A.. Consejo Superior de Investigaciones Científicas; EspañaFil: Ceballos, Olga. Grupo de Estudios Biológicos Ugarra; EspañaWaterbird Society2002-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99128Bertellotti, Néstor Marcelo; Tella Escobedo, José Luis; Godoy, José A.; Blanco, Guillermo; Forero, Manuela G.; et al.; Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions; Waterbird Society; Waterbirds; 25; 4; 12-2002; 479-4841524-4695CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1675/1524-4695(2002)025[0479:DSOMPU]2.0.CO;2info:eu-repo/semantics/altIdentifier/url/https://bioone.org/journals/waterbirds/volume-25/issue-4/1524-4695(2002)025%5b0479%3aDSOMPU%5d2.0.CO%3b2/Determining-Sex-of-Magellanic-Penguins-Using-Molecular-Procedures-and-Discriminant/10.1675/1524-4695(2002)025[0479:DSOMPU]2.0.CO;2.shortinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:21:06Zoai:ri.conicet.gov.ar:11336/99128instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:21:07.117CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions
title Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions
spellingShingle Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions
Bertellotti, Néstor Marcelo
DISCRIMINANT FUNCTIONS
SEXING BIRDS
MAGELLANIC PENGUIN
MOLECULAR SEXING
title_short Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions
title_full Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions
title_fullStr Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions
title_full_unstemmed Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions
title_sort Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions
dc.creator.none.fl_str_mv Bertellotti, Néstor Marcelo
Tella Escobedo, José Luis
Godoy, José A.
Blanco, Guillermo
Forero, Manuela G.
Donázar, José A.
Ceballos, Olga
author Bertellotti, Néstor Marcelo
author_facet Bertellotti, Néstor Marcelo
Tella Escobedo, José Luis
Godoy, José A.
Blanco, Guillermo
Forero, Manuela G.
Donázar, José A.
Ceballos, Olga
author_role author
author2 Tella Escobedo, José Luis
Godoy, José A.
Blanco, Guillermo
Forero, Manuela G.
Donázar, José A.
Ceballos, Olga
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv DISCRIMINANT FUNCTIONS
SEXING BIRDS
MAGELLANIC PENGUIN
MOLECULAR SEXING
topic DISCRIMINANT FUNCTIONS
SEXING BIRDS
MAGELLANIC PENGUIN
MOLECULAR SEXING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Magellanic Penguins (Spheniscus magellanicus) show little sexual dimorphism, and although males are usually larger than females, sexing by direct observation may be difficult, especially in the case of chicks. In this paper we evaluate the utility of four different PCR-based sex determination techniques using genomic DNA for sexing Magellanic Penguins. We found that the primer set designed for sex determination in Collared Flycatcher (Ficedula albicollis) also provided a reliable, simple and convenient sexing procedure for Magellanic Penguins. Additionally, we obtained discriminant functions for sexing adults and chicks, sampled at six colonies differing in size and other ecological characteristics. Discriminant function for adults used two variables, bill length and bill depth that correctly classified 97% of the birds. Discriminant function for chicks included bill length and flipper length and correctly classified 78% of the individuals. Although molecular and biometric approaches could be useful for sexing adult Magellanic Penguins, only molecular procedures proved appropriate for accurately sexing chicks.
Fil: Bertellotti, Néstor Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Tella Escobedo, José Luis. Consejo Superior de Investigaciones Científicas; España
Fil: Godoy, José A.. Consejo Superior de Investigaciones Científicas; España
Fil: Blanco, Guillermo. Universidad de Castilla-La Mancha. Instituto de Investigación en Recursos Cinegéticos; España. Consejo Superior de Investigaciones Científicas; España
Fil: Forero, Manuela G.. Consejo Superior de Investigaciones Científicas; España
Fil: Donázar, José A.. Consejo Superior de Investigaciones Científicas; España
Fil: Ceballos, Olga. Grupo de Estudios Biológicos Ugarra; España
description Magellanic Penguins (Spheniscus magellanicus) show little sexual dimorphism, and although males are usually larger than females, sexing by direct observation may be difficult, especially in the case of chicks. In this paper we evaluate the utility of four different PCR-based sex determination techniques using genomic DNA for sexing Magellanic Penguins. We found that the primer set designed for sex determination in Collared Flycatcher (Ficedula albicollis) also provided a reliable, simple and convenient sexing procedure for Magellanic Penguins. Additionally, we obtained discriminant functions for sexing adults and chicks, sampled at six colonies differing in size and other ecological characteristics. Discriminant function for adults used two variables, bill length and bill depth that correctly classified 97% of the birds. Discriminant function for chicks included bill length and flipper length and correctly classified 78% of the individuals. Although molecular and biometric approaches could be useful for sexing adult Magellanic Penguins, only molecular procedures proved appropriate for accurately sexing chicks.
publishDate 2002
dc.date.none.fl_str_mv 2002-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99128
Bertellotti, Néstor Marcelo; Tella Escobedo, José Luis; Godoy, José A.; Blanco, Guillermo; Forero, Manuela G.; et al.; Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions; Waterbird Society; Waterbirds; 25; 4; 12-2002; 479-484
1524-4695
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99128
identifier_str_mv Bertellotti, Néstor Marcelo; Tella Escobedo, José Luis; Godoy, José A.; Blanco, Guillermo; Forero, Manuela G.; et al.; Determining Sex of Magellanic Penguins Using Molecular Procedures and Discriminant Functions; Waterbird Society; Waterbirds; 25; 4; 12-2002; 479-484
1524-4695
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1675/1524-4695(2002)025[0479:DSOMPU]2.0.CO;2
info:eu-repo/semantics/altIdentifier/url/https://bioone.org/journals/waterbirds/volume-25/issue-4/1524-4695(2002)025%5b0479%3aDSOMPU%5d2.0.CO%3b2/Determining-Sex-of-Magellanic-Penguins-Using-Molecular-Procedures-and-Discriminant/10.1675/1524-4695(2002)025[0479:DSOMPU]2.0.CO;2.short
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Waterbird Society
publisher.none.fl_str_mv Waterbird Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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