Sympathetic innervation regulates macrophage activity in rat with polycystic ovary

Autores
Figueroa, Florencia; Mendoza, Gisela Veronica; Cardozo, Dario Gonzalo; Mohamed, Fabian Heber; Oliveros, Liliana Beatriz; Forneris, Myriam Liliana
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polycystic ovarian syndrome (PCOS) is a low-grade inflammatory disease characterized by hyperandrogenism and ovarian hyperinnervation. The aim of this work is to investigate whether in vivo bilateral superior ovarian nerve (SON) section in adult rats with estradiol valerate-induced PCOS (PCO rats) affects macrophage spleen cells (MΦ) and modifies the steroidogenic ability of their secretions. Culture media of MΦ from PCO rats and PCO rats with SON section (PCO-SON rats) were used to stimulate in vitro intact ovaries. Compared with macrophages PCO, macrophages from PCO-SON rats released less tumor necrosis factor-a and nitric oxide, expressed lower Bax and Nfkb mRNA and showed reduced TUNEL staining. Also, in PCO rats, the SON section decreased kisspeptin and nerve growth factor mRNA expressions, without changes in Trka receptor mRNA levels. Macrophage secretions from PCO-SON rats decreased androstenedione and stimulated progesterone release in PCO ovaries, compared to macrophage secretions from PCO rats. No changes were observed in ovarian estradiol response. These findings emphasize the importance of the SON in spleen MF, since its manipulation leads to secondary modifications of immunological and neural mediators, which might influence ovarian steroidogenesis. In PCO ovaries, the reduction of androstenedione and the improvement of progesterone release induced by PCO-SON MF secretion, might be beneficial considering the hormonal anomalies characteristic of PCOS. We present functional evidence that modulation of the immune-endocrine function by peripheral sympathetic nervous system might have implications for understanding the pathophysiology of PCOS.
Fil: Figueroa, Florencia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Mendoza, Gisela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Cardozo, Dario Gonzalo. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico. Departamento de Ciencias Naturales y Antropológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Oliveros, Liliana Beatriz. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Forneris, Myriam Liliana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Materia
KISSPEPTIN
MACROPHAGES
POLYCYSTIC OVARY
STEROIDOGENESIS
SUPERIOR OVARIAN NERVE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/176409
Acceder
id CONICETDig_9ae3d512883d9110778c81c3ecf414cf
oai_identifier_str oai:ri.conicet.gov.ar:11336/176409
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sympathetic innervation regulates macrophage activity in rat with polycystic ovaryFigueroa, FlorenciaMendoza, Gisela VeronicaCardozo, Dario GonzaloMohamed, Fabian HeberOliveros, Liliana BeatrizForneris, Myriam LilianaKISSPEPTINMACROPHAGESPOLYCYSTIC OVARYSTEROIDOGENESISSUPERIOR OVARIAN NERVEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Polycystic ovarian syndrome (PCOS) is a low-grade inflammatory disease characterized by hyperandrogenism and ovarian hyperinnervation. The aim of this work is to investigate whether in vivo bilateral superior ovarian nerve (SON) section in adult rats with estradiol valerate-induced PCOS (PCO rats) affects macrophage spleen cells (MΦ) and modifies the steroidogenic ability of their secretions. Culture media of MΦ from PCO rats and PCO rats with SON section (PCO-SON rats) were used to stimulate in vitro intact ovaries. Compared with macrophages PCO, macrophages from PCO-SON rats released less tumor necrosis factor-a and nitric oxide, expressed lower Bax and Nfkb mRNA and showed reduced TUNEL staining. Also, in PCO rats, the SON section decreased kisspeptin and nerve growth factor mRNA expressions, without changes in Trka receptor mRNA levels. Macrophage secretions from PCO-SON rats decreased androstenedione and stimulated progesterone release in PCO ovaries, compared to macrophage secretions from PCO rats. No changes were observed in ovarian estradiol response. These findings emphasize the importance of the SON in spleen MF, since its manipulation leads to secondary modifications of immunological and neural mediators, which might influence ovarian steroidogenesis. In PCO ovaries, the reduction of androstenedione and the improvement of progesterone release induced by PCO-SON MF secretion, might be beneficial considering the hormonal anomalies characteristic of PCOS. We present functional evidence that modulation of the immune-endocrine function by peripheral sympathetic nervous system might have implications for understanding the pathophysiology of PCOS.Fil: Figueroa, Florencia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Mendoza, Gisela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Cardozo, Dario Gonzalo. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico. Departamento de Ciencias Naturales y Antropológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Oliveros, Liliana Beatriz. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaFil: Forneris, Myriam Liliana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; ArgentinaBioScientifica2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/176409Figueroa, Florencia; Mendoza, Gisela Veronica; Cardozo, Dario Gonzalo; Mohamed, Fabian Heber; Oliveros, Liliana Beatriz; et al.; Sympathetic innervation regulates macrophage activity in rat with polycystic ovary; BioScientifica; Journal of Endocrinology; 238; 1; 5-2018; 33-450022-0795CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://joe.endocrinology-journals.org/lookup/doi/10.1530/JOE-17-0736info:eu-repo/semantics/altIdentifier/doi/10.1530/JOE-17-0736info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:58:17Zoai:ri.conicet.gov.ar:11336/176409instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:58:17.65CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sympathetic innervation regulates macrophage activity in rat with polycystic ovary
title Sympathetic innervation regulates macrophage activity in rat with polycystic ovary
spellingShingle Sympathetic innervation regulates macrophage activity in rat with polycystic ovary
Figueroa, Florencia
KISSPEPTIN
MACROPHAGES
POLYCYSTIC OVARY
STEROIDOGENESIS
SUPERIOR OVARIAN NERVE
title_short Sympathetic innervation regulates macrophage activity in rat with polycystic ovary
title_full Sympathetic innervation regulates macrophage activity in rat with polycystic ovary
title_fullStr Sympathetic innervation regulates macrophage activity in rat with polycystic ovary
title_full_unstemmed Sympathetic innervation regulates macrophage activity in rat with polycystic ovary
title_sort Sympathetic innervation regulates macrophage activity in rat with polycystic ovary
dc.creator.none.fl_str_mv Figueroa, Florencia
Mendoza, Gisela Veronica
Cardozo, Dario Gonzalo
Mohamed, Fabian Heber
Oliveros, Liliana Beatriz
Forneris, Myriam Liliana
author Figueroa, Florencia
author_facet Figueroa, Florencia
Mendoza, Gisela Veronica
Cardozo, Dario Gonzalo
Mohamed, Fabian Heber
Oliveros, Liliana Beatriz
Forneris, Myriam Liliana
author_role author
author2 Mendoza, Gisela Veronica
Cardozo, Dario Gonzalo
Mohamed, Fabian Heber
Oliveros, Liliana Beatriz
Forneris, Myriam Liliana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv KISSPEPTIN
MACROPHAGES
POLYCYSTIC OVARY
STEROIDOGENESIS
SUPERIOR OVARIAN NERVE
topic KISSPEPTIN
MACROPHAGES
POLYCYSTIC OVARY
STEROIDOGENESIS
SUPERIOR OVARIAN NERVE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Polycystic ovarian syndrome (PCOS) is a low-grade inflammatory disease characterized by hyperandrogenism and ovarian hyperinnervation. The aim of this work is to investigate whether in vivo bilateral superior ovarian nerve (SON) section in adult rats with estradiol valerate-induced PCOS (PCO rats) affects macrophage spleen cells (MΦ) and modifies the steroidogenic ability of their secretions. Culture media of MΦ from PCO rats and PCO rats with SON section (PCO-SON rats) were used to stimulate in vitro intact ovaries. Compared with macrophages PCO, macrophages from PCO-SON rats released less tumor necrosis factor-a and nitric oxide, expressed lower Bax and Nfkb mRNA and showed reduced TUNEL staining. Also, in PCO rats, the SON section decreased kisspeptin and nerve growth factor mRNA expressions, without changes in Trka receptor mRNA levels. Macrophage secretions from PCO-SON rats decreased androstenedione and stimulated progesterone release in PCO ovaries, compared to macrophage secretions from PCO rats. No changes were observed in ovarian estradiol response. These findings emphasize the importance of the SON in spleen MF, since its manipulation leads to secondary modifications of immunological and neural mediators, which might influence ovarian steroidogenesis. In PCO ovaries, the reduction of androstenedione and the improvement of progesterone release induced by PCO-SON MF secretion, might be beneficial considering the hormonal anomalies characteristic of PCOS. We present functional evidence that modulation of the immune-endocrine function by peripheral sympathetic nervous system might have implications for understanding the pathophysiology of PCOS.
Fil: Figueroa, Florencia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Mendoza, Gisela Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Cardozo, Dario Gonzalo. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico. Departamento de Ciencias Naturales y Antropológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Oliveros, Liliana Beatriz. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
Fil: Forneris, Myriam Liliana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas. Laboratorio de Biología de la Reproducción; Argentina
description Polycystic ovarian syndrome (PCOS) is a low-grade inflammatory disease characterized by hyperandrogenism and ovarian hyperinnervation. The aim of this work is to investigate whether in vivo bilateral superior ovarian nerve (SON) section in adult rats with estradiol valerate-induced PCOS (PCO rats) affects macrophage spleen cells (MΦ) and modifies the steroidogenic ability of their secretions. Culture media of MΦ from PCO rats and PCO rats with SON section (PCO-SON rats) were used to stimulate in vitro intact ovaries. Compared with macrophages PCO, macrophages from PCO-SON rats released less tumor necrosis factor-a and nitric oxide, expressed lower Bax and Nfkb mRNA and showed reduced TUNEL staining. Also, in PCO rats, the SON section decreased kisspeptin and nerve growth factor mRNA expressions, without changes in Trka receptor mRNA levels. Macrophage secretions from PCO-SON rats decreased androstenedione and stimulated progesterone release in PCO ovaries, compared to macrophage secretions from PCO rats. No changes were observed in ovarian estradiol response. These findings emphasize the importance of the SON in spleen MF, since its manipulation leads to secondary modifications of immunological and neural mediators, which might influence ovarian steroidogenesis. In PCO ovaries, the reduction of androstenedione and the improvement of progesterone release induced by PCO-SON MF secretion, might be beneficial considering the hormonal anomalies characteristic of PCOS. We present functional evidence that modulation of the immune-endocrine function by peripheral sympathetic nervous system might have implications for understanding the pathophysiology of PCOS.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/176409
Figueroa, Florencia; Mendoza, Gisela Veronica; Cardozo, Dario Gonzalo; Mohamed, Fabian Heber; Oliveros, Liliana Beatriz; et al.; Sympathetic innervation regulates macrophage activity in rat with polycystic ovary; BioScientifica; Journal of Endocrinology; 238; 1; 5-2018; 33-45
0022-0795
CONICET Digital
CONICET
url http://hdl.handle.net/11336/176409
identifier_str_mv Figueroa, Florencia; Mendoza, Gisela Veronica; Cardozo, Dario Gonzalo; Mohamed, Fabian Heber; Oliveros, Liliana Beatriz; et al.; Sympathetic innervation regulates macrophage activity in rat with polycystic ovary; BioScientifica; Journal of Endocrinology; 238; 1; 5-2018; 33-45
0022-0795
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://joe.endocrinology-journals.org/lookup/doi/10.1530/JOE-17-0736
info:eu-repo/semantics/altIdentifier/doi/10.1530/JOE-17-0736
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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