Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction

Autores
Alzamendi, Ana; Giovambattista, Andres; Garcia, Maria Elisa; Rebolledo, Oscar Remigio; Gagliardino, Juan Jose; Spinedi, Eduardo Julio
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim: To test the potential role of PPAR in the endocrine abdominal tissue dysfunction induced by feeding normal rats with a fructose rich diet (FRD) during three weeks. Methodology: Adult normal male rats received a standard commercial diet (CD) or FRD, (10% in drinking water) without or with pioglitazone (PIO) (i.p. 0.25 mg/Kg BW/day; CD-PIO and FRD-PIO). Thereafter, we measured circulating metabolic, endocrine and oxidative stress (OS) markers, abdominal adipose tissue (AAT) mass, leptin (LEP) and plasminogen activator inhibitor-1 (PAI-1) tissue content/expression, and leptin release by isolated adipocytes incubated with different concentrations of insulin. Results: Plasma glucose, insulin, triglyceride, TBARS, LEP and PAI-1 levels were higher in FRD rats; PIO co-administration fully prevented all these increments. AAT adipocytes from FRD rats were larger, secreted a higher amount of LEP and displayed decreased sensitivity to insulin stimulation; these effects were significantly ameliorated by PIO. Whereas AAT LEP and PAI-1 (mRNA) concentrations increased significantly in FRD rats, those of insulin receptor substrate (IRS)-1 and IRS-2 were reduced. PIO co-administration prevented FRD effects on LEP, PAI-1, and IRS-2 (fully), and IRS-1 (partially) mRNAs in AAT. Conclusion: PPARγ would play a relevant role in the development of the FRD-induced metabolic-endocrine dysfunction.
Fil: Alzamendi, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Giovambattista, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Garcia, Maria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Rebolledo, Oscar Remigio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Spinedi, Eduardo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Materia
PPARg
Abdominal Adiposity
Insulin Sensitivity
Experimental Metabolic Syndrome
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/270766
Acceder
id CONICETDig_9844a66455113599bbd1283157e02499
oai_identifier_str oai:ri.conicet.gov.ar:11336/270766
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue DysfunctionAlzamendi, AnaGiovambattista, AndresGarcia, Maria ElisaRebolledo, Oscar RemigioGagliardino, Juan JoseSpinedi, Eduardo JulioPPARgAbdominal AdiposityInsulin SensitivityExperimental Metabolic Syndromehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Aim: To test the potential role of PPAR in the endocrine abdominal tissue dysfunction induced by feeding normal rats with a fructose rich diet (FRD) during three weeks. Methodology: Adult normal male rats received a standard commercial diet (CD) or FRD, (10% in drinking water) without or with pioglitazone (PIO) (i.p. 0.25 mg/Kg BW/day; CD-PIO and FRD-PIO). Thereafter, we measured circulating metabolic, endocrine and oxidative stress (OS) markers, abdominal adipose tissue (AAT) mass, leptin (LEP) and plasminogen activator inhibitor-1 (PAI-1) tissue content/expression, and leptin release by isolated adipocytes incubated with different concentrations of insulin. Results: Plasma glucose, insulin, triglyceride, TBARS, LEP and PAI-1 levels were higher in FRD rats; PIO co-administration fully prevented all these increments. AAT adipocytes from FRD rats were larger, secreted a higher amount of LEP and displayed decreased sensitivity to insulin stimulation; these effects were significantly ameliorated by PIO. Whereas AAT LEP and PAI-1 (mRNA) concentrations increased significantly in FRD rats, those of insulin receptor substrate (IRS)-1 and IRS-2 were reduced. PIO co-administration prevented FRD effects on LEP, PAI-1, and IRS-2 (fully), and IRS-1 (partially) mRNAs in AAT. Conclusion: PPARγ would play a relevant role in the development of the FRD-induced metabolic-endocrine dysfunction.Fil: Alzamendi, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Giovambattista, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Garcia, Maria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaFil: Rebolledo, Oscar Remigio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaFil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaFil: Spinedi, Eduardo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaHindawi Publishing Corporation2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/270766Alzamendi, Ana; Giovambattista, Andres; Garcia, Maria Elisa; Rebolledo, Oscar Remigio; Gagliardino, Juan Jose; et al.; Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction; Hindawi Publishing Corporation; PPAR Research; 2012; 8-2012; 1-91687-47571687-4765CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1155/2012/259093info:eu-repo/semantics/altIdentifier/doi/10.1155/2012/259093info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:44:53Zoai:ri.conicet.gov.ar:11336/270766instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:44:54.131CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction
title Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction
spellingShingle Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction
Alzamendi, Ana
PPARg
Abdominal Adiposity
Insulin Sensitivity
Experimental Metabolic Syndrome
title_short Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction
title_full Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction
title_fullStr Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction
title_full_unstemmed Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction
title_sort Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction
dc.creator.none.fl_str_mv Alzamendi, Ana
Giovambattista, Andres
Garcia, Maria Elisa
Rebolledo, Oscar Remigio
Gagliardino, Juan Jose
Spinedi, Eduardo Julio
author Alzamendi, Ana
author_facet Alzamendi, Ana
Giovambattista, Andres
Garcia, Maria Elisa
Rebolledo, Oscar Remigio
Gagliardino, Juan Jose
Spinedi, Eduardo Julio
author_role author
author2 Giovambattista, Andres
Garcia, Maria Elisa
Rebolledo, Oscar Remigio
Gagliardino, Juan Jose
Spinedi, Eduardo Julio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv PPARg
Abdominal Adiposity
Insulin Sensitivity
Experimental Metabolic Syndrome
topic PPARg
Abdominal Adiposity
Insulin Sensitivity
Experimental Metabolic Syndrome
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aim: To test the potential role of PPAR in the endocrine abdominal tissue dysfunction induced by feeding normal rats with a fructose rich diet (FRD) during three weeks. Methodology: Adult normal male rats received a standard commercial diet (CD) or FRD, (10% in drinking water) without or with pioglitazone (PIO) (i.p. 0.25 mg/Kg BW/day; CD-PIO and FRD-PIO). Thereafter, we measured circulating metabolic, endocrine and oxidative stress (OS) markers, abdominal adipose tissue (AAT) mass, leptin (LEP) and plasminogen activator inhibitor-1 (PAI-1) tissue content/expression, and leptin release by isolated adipocytes incubated with different concentrations of insulin. Results: Plasma glucose, insulin, triglyceride, TBARS, LEP and PAI-1 levels were higher in FRD rats; PIO co-administration fully prevented all these increments. AAT adipocytes from FRD rats were larger, secreted a higher amount of LEP and displayed decreased sensitivity to insulin stimulation; these effects were significantly ameliorated by PIO. Whereas AAT LEP and PAI-1 (mRNA) concentrations increased significantly in FRD rats, those of insulin receptor substrate (IRS)-1 and IRS-2 were reduced. PIO co-administration prevented FRD effects on LEP, PAI-1, and IRS-2 (fully), and IRS-1 (partially) mRNAs in AAT. Conclusion: PPARγ would play a relevant role in the development of the FRD-induced metabolic-endocrine dysfunction.
Fil: Alzamendi, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Giovambattista, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Garcia, Maria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Rebolledo, Oscar Remigio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Gagliardino, Juan Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Spinedi, Eduardo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
description Aim: To test the potential role of PPAR in the endocrine abdominal tissue dysfunction induced by feeding normal rats with a fructose rich diet (FRD) during three weeks. Methodology: Adult normal male rats received a standard commercial diet (CD) or FRD, (10% in drinking water) without or with pioglitazone (PIO) (i.p. 0.25 mg/Kg BW/day; CD-PIO and FRD-PIO). Thereafter, we measured circulating metabolic, endocrine and oxidative stress (OS) markers, abdominal adipose tissue (AAT) mass, leptin (LEP) and plasminogen activator inhibitor-1 (PAI-1) tissue content/expression, and leptin release by isolated adipocytes incubated with different concentrations of insulin. Results: Plasma glucose, insulin, triglyceride, TBARS, LEP and PAI-1 levels were higher in FRD rats; PIO co-administration fully prevented all these increments. AAT adipocytes from FRD rats were larger, secreted a higher amount of LEP and displayed decreased sensitivity to insulin stimulation; these effects were significantly ameliorated by PIO. Whereas AAT LEP and PAI-1 (mRNA) concentrations increased significantly in FRD rats, those of insulin receptor substrate (IRS)-1 and IRS-2 were reduced. PIO co-administration prevented FRD effects on LEP, PAI-1, and IRS-2 (fully), and IRS-1 (partially) mRNAs in AAT. Conclusion: PPARγ would play a relevant role in the development of the FRD-induced metabolic-endocrine dysfunction.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/270766
Alzamendi, Ana; Giovambattista, Andres; Garcia, Maria Elisa; Rebolledo, Oscar Remigio; Gagliardino, Juan Jose; et al.; Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction; Hindawi Publishing Corporation; PPAR Research; 2012; 8-2012; 1-9
1687-4757
1687-4765
CONICET Digital
CONICET
url http://hdl.handle.net/11336/270766
identifier_str_mv Alzamendi, Ana; Giovambattista, Andres; Garcia, Maria Elisa; Rebolledo, Oscar Remigio; Gagliardino, Juan Jose; et al.; Effect of Pioglitazone on the Fructose-Induced Abdominal Adipose Tissue Dysfunction; Hindawi Publishing Corporation; PPAR Research; 2012; 8-2012; 1-9
1687-4757
1687-4765
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1155/2012/259093
info:eu-repo/semantics/altIdentifier/doi/10.1155/2012/259093
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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