Role of ghrelin in fertilization, early embryo development, and implantation periods

Autores
Luque, Eugenia Mercedes; Torres, Pedro Javier; de Loredo, Nicolás; Vincenti, Laura María; Stutz, Graciela; Santillán, María Emilia; Ruiz, Ruben Daniel; Fiol, Marta Haydee; Martini, Ana Carolina
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In order to clarify the physiological role of ghrelin in gestation, we evaluated the effects of administration of exogenous ghrelin (2 or 4 nmol/animal per day) or its antagonist (6 nmol/animal per day of (D-Lys3)GHRP6) on fertilization, early embryo development, and implantation periods in mice. Three experiments were performed, treating female mice with ghrelin or its antagonist: i) starting from 1 week before copulation to 12 h after copulation, mice were killed at day 18 of gestation; ii) since ovulation induction until 80 h later, when we retrieved the embryos from oviducts/uterus, and iii) starting from days 3 to 7 of gestation (peri-implantation), mice were killed at day 18. In experiments 1 and 3, the antagonist and/or the highest dose of ghrelin significantly increased the percentage of atrophied fetuses and that of females exhibiting this finding or a higher amount of corpora lutea compared with fetuses (nCL/nF) (experiment 3: higher nCL/nF-atrophied fetuses: ghrelin 4, 71.4–71.4% and antagonist, 75.0–62.5% vs ghrelin 2, 46.2K15.4% and control, 10–0.0%; nZ7–13 females/group; P!0.01). In experiment 2, the antagonist diminished the fertilization rate, and both, ghrelin and the antagonist, delayed embryo development (blastocysts: ghrelin 2, 62.5%; ghrelin 4, 50.6%; and antagonist, 61.0% vs control 78.4%; nZ82–102 embryos/treatment; P!0.0001). In experiment 3, additionally, ghrelin (4 nmol/day) and the antagonist significantly diminished the weight gain of fetuses and dams during pregnancy. Our results indicate that not only hyperghrelinemia but also the inhibition of the endogenous ghrelin effects exerts negative effects on the fertilization, implantation, and embryo/fetal development periods, supporting the hypothesis that ghrelin (in ‘adequate’ concentrations) has a physiological role in early gestational events.
Fil: Luque, Eugenia Mercedes. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Torres, Pedro Javier. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: de Loredo, Nicolás. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Vincenti, Laura María. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Stutz, Graciela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Santillán, María Emilia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Ruiz, Ruben Daniel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Fiol, Marta Haydee. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Martini, Ana Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Materia
Reproduction
Ghrelin
Fertilization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11706
Acceder
id CONICETDig_976ddbee0b0f4d53b1a68d20afe068e4
oai_identifier_str oai:ri.conicet.gov.ar:11336/11706
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of ghrelin in fertilization, early embryo development, and implantation periodsLuque, Eugenia MercedesTorres, Pedro Javierde Loredo, NicolásVincenti, Laura MaríaStutz, GracielaSantillán, María EmiliaRuiz, Ruben DanielFiol, Marta HaydeeMartini, Ana CarolinaReproductionGhrelinFertilizationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In order to clarify the physiological role of ghrelin in gestation, we evaluated the effects of administration of exogenous ghrelin (2 or 4 nmol/animal per day) or its antagonist (6 nmol/animal per day of (D-Lys3)GHRP6) on fertilization, early embryo development, and implantation periods in mice. Three experiments were performed, treating female mice with ghrelin or its antagonist: i) starting from 1 week before copulation to 12 h after copulation, mice were killed at day 18 of gestation; ii) since ovulation induction until 80 h later, when we retrieved the embryos from oviducts/uterus, and iii) starting from days 3 to 7 of gestation (peri-implantation), mice were killed at day 18. In experiments 1 and 3, the antagonist and/or the highest dose of ghrelin significantly increased the percentage of atrophied fetuses and that of females exhibiting this finding or a higher amount of corpora lutea compared with fetuses (nCL/nF) (experiment 3: higher nCL/nF-atrophied fetuses: ghrelin 4, 71.4–71.4% and antagonist, 75.0–62.5% vs ghrelin 2, 46.2K15.4% and control, 10–0.0%; nZ7–13 females/group; P!0.01). In experiment 2, the antagonist diminished the fertilization rate, and both, ghrelin and the antagonist, delayed embryo development (blastocysts: ghrelin 2, 62.5%; ghrelin 4, 50.6%; and antagonist, 61.0% vs control 78.4%; nZ82–102 embryos/treatment; P!0.0001). In experiment 3, additionally, ghrelin (4 nmol/day) and the antagonist significantly diminished the weight gain of fetuses and dams during pregnancy. Our results indicate that not only hyperghrelinemia but also the inhibition of the endogenous ghrelin effects exerts negative effects on the fertilization, implantation, and embryo/fetal development periods, supporting the hypothesis that ghrelin (in ‘adequate’ concentrations) has a physiological role in early gestational events.Fil: Luque, Eugenia Mercedes. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Torres, Pedro Javier. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: de Loredo, Nicolás. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Vincenti, Laura María. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Stutz, Graciela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Santillán, María Emilia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Ruiz, Ruben Daniel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Fiol, Marta Haydee. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Martini, Ana Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaBioscientifica2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11706Luque, Eugenia Mercedes; Torres, Pedro Javier; de Loredo, Nicolás; Vincenti, Laura María; Stutz, Graciela; et al.; Role of ghrelin in fertilization, early embryo development, and implantation periods; Bioscientifica; Reproduction; 148; 6-2015; 159-1671470-1626enginfo:eu-repo/semantics/altIdentifier/doi/10.1530/REP-14-0129info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/148/2/159info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T09:33:36Zoai:ri.conicet.gov.ar:11336/11706instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 09:33:36.883CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of ghrelin in fertilization, early embryo development, and implantation periods
title Role of ghrelin in fertilization, early embryo development, and implantation periods
spellingShingle Role of ghrelin in fertilization, early embryo development, and implantation periods
Luque, Eugenia Mercedes
Reproduction
Ghrelin
Fertilization
title_short Role of ghrelin in fertilization, early embryo development, and implantation periods
title_full Role of ghrelin in fertilization, early embryo development, and implantation periods
title_fullStr Role of ghrelin in fertilization, early embryo development, and implantation periods
title_full_unstemmed Role of ghrelin in fertilization, early embryo development, and implantation periods
title_sort Role of ghrelin in fertilization, early embryo development, and implantation periods
dc.creator.none.fl_str_mv Luque, Eugenia Mercedes
Torres, Pedro Javier
de Loredo, Nicolás
Vincenti, Laura María
Stutz, Graciela
Santillán, María Emilia
Ruiz, Ruben Daniel
Fiol, Marta Haydee
Martini, Ana Carolina
author Luque, Eugenia Mercedes
author_facet Luque, Eugenia Mercedes
Torres, Pedro Javier
de Loredo, Nicolás
Vincenti, Laura María
Stutz, Graciela
Santillán, María Emilia
Ruiz, Ruben Daniel
Fiol, Marta Haydee
Martini, Ana Carolina
author_role author
author2 Torres, Pedro Javier
de Loredo, Nicolás
Vincenti, Laura María
Stutz, Graciela
Santillán, María Emilia
Ruiz, Ruben Daniel
Fiol, Marta Haydee
Martini, Ana Carolina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Reproduction
Ghrelin
Fertilization
topic Reproduction
Ghrelin
Fertilization
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In order to clarify the physiological role of ghrelin in gestation, we evaluated the effects of administration of exogenous ghrelin (2 or 4 nmol/animal per day) or its antagonist (6 nmol/animal per day of (D-Lys3)GHRP6) on fertilization, early embryo development, and implantation periods in mice. Three experiments were performed, treating female mice with ghrelin or its antagonist: i) starting from 1 week before copulation to 12 h after copulation, mice were killed at day 18 of gestation; ii) since ovulation induction until 80 h later, when we retrieved the embryos from oviducts/uterus, and iii) starting from days 3 to 7 of gestation (peri-implantation), mice were killed at day 18. In experiments 1 and 3, the antagonist and/or the highest dose of ghrelin significantly increased the percentage of atrophied fetuses and that of females exhibiting this finding or a higher amount of corpora lutea compared with fetuses (nCL/nF) (experiment 3: higher nCL/nF-atrophied fetuses: ghrelin 4, 71.4–71.4% and antagonist, 75.0–62.5% vs ghrelin 2, 46.2K15.4% and control, 10–0.0%; nZ7–13 females/group; P!0.01). In experiment 2, the antagonist diminished the fertilization rate, and both, ghrelin and the antagonist, delayed embryo development (blastocysts: ghrelin 2, 62.5%; ghrelin 4, 50.6%; and antagonist, 61.0% vs control 78.4%; nZ82–102 embryos/treatment; P!0.0001). In experiment 3, additionally, ghrelin (4 nmol/day) and the antagonist significantly diminished the weight gain of fetuses and dams during pregnancy. Our results indicate that not only hyperghrelinemia but also the inhibition of the endogenous ghrelin effects exerts negative effects on the fertilization, implantation, and embryo/fetal development periods, supporting the hypothesis that ghrelin (in ‘adequate’ concentrations) has a physiological role in early gestational events.
Fil: Luque, Eugenia Mercedes. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Torres, Pedro Javier. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: de Loredo, Nicolás. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Vincenti, Laura María. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Stutz, Graciela. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Santillán, María Emilia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Ruiz, Ruben Daniel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Fiol, Marta Haydee. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
Fil: Martini, Ana Carolina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina
description In order to clarify the physiological role of ghrelin in gestation, we evaluated the effects of administration of exogenous ghrelin (2 or 4 nmol/animal per day) or its antagonist (6 nmol/animal per day of (D-Lys3)GHRP6) on fertilization, early embryo development, and implantation periods in mice. Three experiments were performed, treating female mice with ghrelin or its antagonist: i) starting from 1 week before copulation to 12 h after copulation, mice were killed at day 18 of gestation; ii) since ovulation induction until 80 h later, when we retrieved the embryos from oviducts/uterus, and iii) starting from days 3 to 7 of gestation (peri-implantation), mice were killed at day 18. In experiments 1 and 3, the antagonist and/or the highest dose of ghrelin significantly increased the percentage of atrophied fetuses and that of females exhibiting this finding or a higher amount of corpora lutea compared with fetuses (nCL/nF) (experiment 3: higher nCL/nF-atrophied fetuses: ghrelin 4, 71.4–71.4% and antagonist, 75.0–62.5% vs ghrelin 2, 46.2K15.4% and control, 10–0.0%; nZ7–13 females/group; P!0.01). In experiment 2, the antagonist diminished the fertilization rate, and both, ghrelin and the antagonist, delayed embryo development (blastocysts: ghrelin 2, 62.5%; ghrelin 4, 50.6%; and antagonist, 61.0% vs control 78.4%; nZ82–102 embryos/treatment; P!0.0001). In experiment 3, additionally, ghrelin (4 nmol/day) and the antagonist significantly diminished the weight gain of fetuses and dams during pregnancy. Our results indicate that not only hyperghrelinemia but also the inhibition of the endogenous ghrelin effects exerts negative effects on the fertilization, implantation, and embryo/fetal development periods, supporting the hypothesis that ghrelin (in ‘adequate’ concentrations) has a physiological role in early gestational events.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11706
Luque, Eugenia Mercedes; Torres, Pedro Javier; de Loredo, Nicolás; Vincenti, Laura María; Stutz, Graciela; et al.; Role of ghrelin in fertilization, early embryo development, and implantation periods; Bioscientifica; Reproduction; 148; 6-2015; 159-167
1470-1626
url http://hdl.handle.net/11336/11706
identifier_str_mv Luque, Eugenia Mercedes; Torres, Pedro Javier; de Loredo, Nicolás; Vincenti, Laura María; Stutz, Graciela; et al.; Role of ghrelin in fertilization, early embryo development, and implantation periods; Bioscientifica; Reproduction; 148; 6-2015; 159-167
1470-1626
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-14-0129
info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/148/2/159
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bioscientifica
publisher.none.fl_str_mv Bioscientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847976717557170176
score 13.082534

Publicaciones similares