Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role

Autores
Ragone, María Inés; Torres, N. S.; Consolini, A. E.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Abstract Aim: To study the role of mitochondria in the recovery of guinea-pig hearts exposed to high-K+-cardioplegia (CPG) and ischaemia/reperfusion (I/R) Methods: We measured contractility and heat release in perfused guineapig hearts and cytosolic and mitochondrial Ca2+ by epifluorescence and confocal microscopy in isolated cardiomyocytes loaded with Fluo-4 or Rhod-2. Results: In hearts, CPG increased the postischaemic contractile recovery, and this was potentiated by the mNCX blocker clonazepam and the mKATP opener diazoxide, which also prevented the fall in muscle economy. Moreover, CPG prevented the stunning induced by ouabain, which was reduced by clonazepam. In cardiomyocytes, CPG increased fluorescent signals of cytosolic and mitochondrial Ca2+, while the addition of a mNCX blocker (CGP37157) increased cytosolic but reduced mitochondrial [Ca2+]. Ouabain in CPG increased cytosolic Ca2+ and resting heat, but the addition of CGP37157 reduced them, as well as mitochondrial Ca2+. Conclusions: CPG, diazoxide and clonazepam improve postischaemic recovery, respectively, by increasing the Ca2+ cycling and by reducing the mitochondrial Ca2+ uptake either by uniporter or by mNCX. The mitochondria compete with the leaky sarcoplasmic reticulum (SR) as sink of Ca2+ in guinea-pig hearts, affecting the postischaemic contractility. CPG also prevented the ouabain-induced dysfunction by avoiding the Ca2+ overload. Ouabain reduced the synergism between CPG and clonazepam suggesting that [Na+]i and SR load influence the mNCX role.
Fil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Torres, N. S.. Cardiovascular Research And Training Institute; Estados Unidos
Fil: Consolini, A. E.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
Materia
CA2+
CALORIMETRY
CARDIOPLEGIA
HEART
ISCHAEMIA-REPERFUSION
MITOCHONDRIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/196171
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/196171
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial roleRagone, María InésTorres, N. S.Consolini, A. E.CA2+CALORIMETRYCARDIOPLEGIAHEARTISCHAEMIA-REPERFUSIONMITOCHONDRIAhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Abstract Aim: To study the role of mitochondria in the recovery of guinea-pig hearts exposed to high-K+-cardioplegia (CPG) and ischaemia/reperfusion (I/R) Methods: We measured contractility and heat release in perfused guineapig hearts and cytosolic and mitochondrial Ca2+ by epifluorescence and confocal microscopy in isolated cardiomyocytes loaded with Fluo-4 or Rhod-2. Results: In hearts, CPG increased the postischaemic contractile recovery, and this was potentiated by the mNCX blocker clonazepam and the mKATP opener diazoxide, which also prevented the fall in muscle economy. Moreover, CPG prevented the stunning induced by ouabain, which was reduced by clonazepam. In cardiomyocytes, CPG increased fluorescent signals of cytosolic and mitochondrial Ca2+, while the addition of a mNCX blocker (CGP37157) increased cytosolic but reduced mitochondrial [Ca2+]. Ouabain in CPG increased cytosolic Ca2+ and resting heat, but the addition of CGP37157 reduced them, as well as mitochondrial Ca2+. Conclusions: CPG, diazoxide and clonazepam improve postischaemic recovery, respectively, by increasing the Ca2+ cycling and by reducing the mitochondrial Ca2+ uptake either by uniporter or by mNCX. The mitochondria compete with the leaky sarcoplasmic reticulum (SR) as sink of Ca2+ in guinea-pig hearts, affecting the postischaemic contractility. CPG also prevented the ouabain-induced dysfunction by avoiding the Ca2+ overload. Ouabain reduced the synergism between CPG and clonazepam suggesting that [Na+]i and SR load influence the mNCX role.Fil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Torres, N. S.. Cardiovascular Research And Training Institute; Estados UnidosFil: Consolini, A. E.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaWiley Blackwell Publishing, Inc2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/196171Ragone, María Inés; Torres, N. S.; Consolini, A. E.; Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role; Wiley Blackwell Publishing, Inc; Acta Physiologica; 207; 2; 11-2012; 369-3841748-1708CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/apha.12027info:eu-repo/semantics/altIdentifier/doi/10.1111/apha.12027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:57Zoai:ri.conicet.gov.ar:11336/196171instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:58.011CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role
title Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role
spellingShingle Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role
Ragone, María Inés
CA2+
CALORIMETRY
CARDIOPLEGIA
HEART
ISCHAEMIA-REPERFUSION
MITOCHONDRIA
title_short Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role
title_full Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role
title_fullStr Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role
title_full_unstemmed Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role
title_sort Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role
dc.creator.none.fl_str_mv Ragone, María Inés
Torres, N. S.
Consolini, A. E.
author Ragone, María Inés
author_facet Ragone, María Inés
Torres, N. S.
Consolini, A. E.
author_role author
author2 Torres, N. S.
Consolini, A. E.
author2_role author
author
dc.subject.none.fl_str_mv CA2+
CALORIMETRY
CARDIOPLEGIA
HEART
ISCHAEMIA-REPERFUSION
MITOCHONDRIA
topic CA2+
CALORIMETRY
CARDIOPLEGIA
HEART
ISCHAEMIA-REPERFUSION
MITOCHONDRIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Abstract Aim: To study the role of mitochondria in the recovery of guinea-pig hearts exposed to high-K+-cardioplegia (CPG) and ischaemia/reperfusion (I/R) Methods: We measured contractility and heat release in perfused guineapig hearts and cytosolic and mitochondrial Ca2+ by epifluorescence and confocal microscopy in isolated cardiomyocytes loaded with Fluo-4 or Rhod-2. Results: In hearts, CPG increased the postischaemic contractile recovery, and this was potentiated by the mNCX blocker clonazepam and the mKATP opener diazoxide, which also prevented the fall in muscle economy. Moreover, CPG prevented the stunning induced by ouabain, which was reduced by clonazepam. In cardiomyocytes, CPG increased fluorescent signals of cytosolic and mitochondrial Ca2+, while the addition of a mNCX blocker (CGP37157) increased cytosolic but reduced mitochondrial [Ca2+]. Ouabain in CPG increased cytosolic Ca2+ and resting heat, but the addition of CGP37157 reduced them, as well as mitochondrial Ca2+. Conclusions: CPG, diazoxide and clonazepam improve postischaemic recovery, respectively, by increasing the Ca2+ cycling and by reducing the mitochondrial Ca2+ uptake either by uniporter or by mNCX. The mitochondria compete with the leaky sarcoplasmic reticulum (SR) as sink of Ca2+ in guinea-pig hearts, affecting the postischaemic contractility. CPG also prevented the ouabain-induced dysfunction by avoiding the Ca2+ overload. Ouabain reduced the synergism between CPG and clonazepam suggesting that [Na+]i and SR load influence the mNCX role.
Fil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Torres, N. S.. Cardiovascular Research And Training Institute; Estados Unidos
Fil: Consolini, A. E.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina
description Abstract Aim: To study the role of mitochondria in the recovery of guinea-pig hearts exposed to high-K+-cardioplegia (CPG) and ischaemia/reperfusion (I/R) Methods: We measured contractility and heat release in perfused guineapig hearts and cytosolic and mitochondrial Ca2+ by epifluorescence and confocal microscopy in isolated cardiomyocytes loaded with Fluo-4 or Rhod-2. Results: In hearts, CPG increased the postischaemic contractile recovery, and this was potentiated by the mNCX blocker clonazepam and the mKATP opener diazoxide, which also prevented the fall in muscle economy. Moreover, CPG prevented the stunning induced by ouabain, which was reduced by clonazepam. In cardiomyocytes, CPG increased fluorescent signals of cytosolic and mitochondrial Ca2+, while the addition of a mNCX blocker (CGP37157) increased cytosolic but reduced mitochondrial [Ca2+]. Ouabain in CPG increased cytosolic Ca2+ and resting heat, but the addition of CGP37157 reduced them, as well as mitochondrial Ca2+. Conclusions: CPG, diazoxide and clonazepam improve postischaemic recovery, respectively, by increasing the Ca2+ cycling and by reducing the mitochondrial Ca2+ uptake either by uniporter or by mNCX. The mitochondria compete with the leaky sarcoplasmic reticulum (SR) as sink of Ca2+ in guinea-pig hearts, affecting the postischaemic contractility. CPG also prevented the ouabain-induced dysfunction by avoiding the Ca2+ overload. Ouabain reduced the synergism between CPG and clonazepam suggesting that [Na+]i and SR load influence the mNCX role.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/196171
Ragone, María Inés; Torres, N. S.; Consolini, A. E.; Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role; Wiley Blackwell Publishing, Inc; Acta Physiologica; 207; 2; 11-2012; 369-384
1748-1708
CONICET Digital
CONICET
url http://hdl.handle.net/11336/196171
identifier_str_mv Ragone, María Inés; Torres, N. S.; Consolini, A. E.; Energetic study of cardioplegic hearts under ischaemia/reperfusion and [Ca2+] changes in cardiomyocytes of guinea-pig: Mitochondrial role; Wiley Blackwell Publishing, Inc; Acta Physiologica; 207; 2; 11-2012; 369-384
1748-1708
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/apha.12027
info:eu-repo/semantics/altIdentifier/doi/10.1111/apha.12027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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