Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces

Autores
Paez, Julieta Irene; Brunetti, Veronica; Strumia, Miriam Cristina; Becherer, Tobias; Solomun, Tihomir; Miguel, Jorge; Calderon, Marcelo; Haag, Rainer
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dendritic polyglycerol (PG) functionalized surfaces represent a good alternative for preparation of protein resistant materials, whose versatility can be enhanced by conferring them the ability to bind particular biomolecules of interest to the surface. In this work, PG derivatives bearing disulfide and different loadings of amino moieties (0–14%) were synthesized and attached to gold surfaces. The modified surfaces were characterized by means of infrared reflection adsorption spectroscopy (FT-IRRAS), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. The protein resistance properties of the PG-modified surfaces were evaluated by surface plasmon resonance (SPR) spectroscopy using fibrinogen, albumin, pepsin, and lysozyme as model proteins. The availability and accessibility of the amino groups to bind biomolecules were assessed by fluorescence measurements. This study demonstrates that PG-coated surfaces with amino contents up to 9% still show very good protein resistant properties. At the same time, the amino moieties on the surface are available and reactive for selective ligand attachment. By fluorescence labeled DNA hybridization, the high selectivity of these functional surfaces could be demonstrated.
Fil: Paez, Julieta Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina
Fil: Brunetti, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones En Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones En Físico-química de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; Argentina
Fil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina
Fil: Becherer, Tobias. Universität Zu Berlin; Alemania
Fil: Solomun, Tihomir. Universität Zu Berlin; Alemania
Fil: Miguel, Jorge. Universität Zu Berlin; Alemania
Fil: Calderon, Marcelo. Universität Zu Berlin; Alemania
Fil: Haag, Rainer. Universität Zu Berlin; Alemania
Materia
Dendritic Polyglycerolamine
Antifouling
Coating
Gold Nanoparticles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/17545
Acceder
id CONICETDig_96f9fb84c58fa438032389952a1a194b
oai_identifier_str oai:ri.conicet.gov.ar:11336/17545
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dendritic polyglycerolamine as a functional antifouling coating of gold surfacesPaez, Julieta IreneBrunetti, VeronicaStrumia, Miriam CristinaBecherer, TobiasSolomun, TihomirMiguel, JorgeCalderon, MarceloHaag, RainerDendritic PolyglycerolamineAntifoulingCoatingGold Nanoparticleshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Dendritic polyglycerol (PG) functionalized surfaces represent a good alternative for preparation of protein resistant materials, whose versatility can be enhanced by conferring them the ability to bind particular biomolecules of interest to the surface. In this work, PG derivatives bearing disulfide and different loadings of amino moieties (0–14%) were synthesized and attached to gold surfaces. The modified surfaces were characterized by means of infrared reflection adsorption spectroscopy (FT-IRRAS), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. The protein resistance properties of the PG-modified surfaces were evaluated by surface plasmon resonance (SPR) spectroscopy using fibrinogen, albumin, pepsin, and lysozyme as model proteins. The availability and accessibility of the amino groups to bind biomolecules were assessed by fluorescence measurements. This study demonstrates that PG-coated surfaces with amino contents up to 9% still show very good protein resistant properties. At the same time, the amino moieties on the surface are available and reactive for selective ligand attachment. By fluorescence labeled DNA hybridization, the high selectivity of these functional surfaces could be demonstrated.Fil: Paez, Julieta Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; ArgentinaFil: Brunetti, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones En Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones En Físico-química de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; ArgentinaFil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; ArgentinaFil: Becherer, Tobias. Universität Zu Berlin; AlemaniaFil: Solomun, Tihomir. Universität Zu Berlin; AlemaniaFil: Miguel, Jorge. Universität Zu Berlin; AlemaniaFil: Calderon, Marcelo. Universität Zu Berlin; AlemaniaFil: Haag, Rainer. Universität Zu Berlin; AlemaniaRoyal Society Of Chemistry2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/17545Paez, Julieta Irene; Brunetti, Veronica; Strumia, Miriam Cristina; Becherer, Tobias; Solomun, Tihomir; et al.; Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces; Royal Society Of Chemistry; Journal Of Materials Chemistry; 22; 37; 5-2012; 19488-194970959-9428enginfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2012/jm/c2jm32486einfo:eu-repo/semantics/altIdentifier/doi/10.1039/c2jm32486einfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:40:24Zoai:ri.conicet.gov.ar:11336/17545instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:40:24.908CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces
title Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces
spellingShingle Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces
Paez, Julieta Irene
Dendritic Polyglycerolamine
Antifouling
Coating
Gold Nanoparticles
title_short Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces
title_full Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces
title_fullStr Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces
title_full_unstemmed Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces
title_sort Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces
dc.creator.none.fl_str_mv Paez, Julieta Irene
Brunetti, Veronica
Strumia, Miriam Cristina
Becherer, Tobias
Solomun, Tihomir
Miguel, Jorge
Calderon, Marcelo
Haag, Rainer
author Paez, Julieta Irene
author_facet Paez, Julieta Irene
Brunetti, Veronica
Strumia, Miriam Cristina
Becherer, Tobias
Solomun, Tihomir
Miguel, Jorge
Calderon, Marcelo
Haag, Rainer
author_role author
author2 Brunetti, Veronica
Strumia, Miriam Cristina
Becherer, Tobias
Solomun, Tihomir
Miguel, Jorge
Calderon, Marcelo
Haag, Rainer
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dendritic Polyglycerolamine
Antifouling
Coating
Gold Nanoparticles
topic Dendritic Polyglycerolamine
Antifouling
Coating
Gold Nanoparticles
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Dendritic polyglycerol (PG) functionalized surfaces represent a good alternative for preparation of protein resistant materials, whose versatility can be enhanced by conferring them the ability to bind particular biomolecules of interest to the surface. In this work, PG derivatives bearing disulfide and different loadings of amino moieties (0–14%) were synthesized and attached to gold surfaces. The modified surfaces were characterized by means of infrared reflection adsorption spectroscopy (FT-IRRAS), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. The protein resistance properties of the PG-modified surfaces were evaluated by surface plasmon resonance (SPR) spectroscopy using fibrinogen, albumin, pepsin, and lysozyme as model proteins. The availability and accessibility of the amino groups to bind biomolecules were assessed by fluorescence measurements. This study demonstrates that PG-coated surfaces with amino contents up to 9% still show very good protein resistant properties. At the same time, the amino moieties on the surface are available and reactive for selective ligand attachment. By fluorescence labeled DNA hybridization, the high selectivity of these functional surfaces could be demonstrated.
Fil: Paez, Julieta Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina
Fil: Brunetti, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones En Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones En Físico-química de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; Argentina
Fil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina
Fil: Becherer, Tobias. Universität Zu Berlin; Alemania
Fil: Solomun, Tihomir. Universität Zu Berlin; Alemania
Fil: Miguel, Jorge. Universität Zu Berlin; Alemania
Fil: Calderon, Marcelo. Universität Zu Berlin; Alemania
Fil: Haag, Rainer. Universität Zu Berlin; Alemania
description Dendritic polyglycerol (PG) functionalized surfaces represent a good alternative for preparation of protein resistant materials, whose versatility can be enhanced by conferring them the ability to bind particular biomolecules of interest to the surface. In this work, PG derivatives bearing disulfide and different loadings of amino moieties (0–14%) were synthesized and attached to gold surfaces. The modified surfaces were characterized by means of infrared reflection adsorption spectroscopy (FT-IRRAS), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. The protein resistance properties of the PG-modified surfaces were evaluated by surface plasmon resonance (SPR) spectroscopy using fibrinogen, albumin, pepsin, and lysozyme as model proteins. The availability and accessibility of the amino groups to bind biomolecules were assessed by fluorescence measurements. This study demonstrates that PG-coated surfaces with amino contents up to 9% still show very good protein resistant properties. At the same time, the amino moieties on the surface are available and reactive for selective ligand attachment. By fluorescence labeled DNA hybridization, the high selectivity of these functional surfaces could be demonstrated.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/17545
Paez, Julieta Irene; Brunetti, Veronica; Strumia, Miriam Cristina; Becherer, Tobias; Solomun, Tihomir; et al.; Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces; Royal Society Of Chemistry; Journal Of Materials Chemistry; 22; 37; 5-2012; 19488-19497
0959-9428
url http://hdl.handle.net/11336/17545
identifier_str_mv Paez, Julieta Irene; Brunetti, Veronica; Strumia, Miriam Cristina; Becherer, Tobias; Solomun, Tihomir; et al.; Dendritic polyglycerolamine as a functional antifouling coating of gold surfaces; Royal Society Of Chemistry; Journal Of Materials Chemistry; 22; 37; 5-2012; 19488-19497
0959-9428
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2012/jm/c2jm32486e
info:eu-repo/semantics/altIdentifier/doi/10.1039/c2jm32486e
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society Of Chemistry
publisher.none.fl_str_mv Royal Society Of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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