Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus

Autores
Croce, Anna Cleta; Garbelli, Anna; Moyano, Andrea del Huerto; Soldano, Sara; Tejeda Guzmán, Carlos; Missirlis, Fanis; Scolari, Francesca
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Malpighian tubules (MTs) are arthropod excretory organs crucial for the osmoregulation, detoxification and excretion of xenobiotics and metabolic wastes, which include tryptophan degradation products along the kynurenine (KYN) pathway. Specifically, the toxic intermediate 3-hydroxy kynurenine (3-HK) is metabolized through transamination to xanthurenic acid or in the synthesis of ommochrome pigments. Early investigations in Drosophila larval fat bodies revealed an intracellular autofluorescence (AF) that depended on tryptophan administration. Subsequent observations documented AF changes in the MTs of Drosophila eye-color mutants genetically affecting the conversion of tryptophan to KYN or 3-HK and the intracellular availability of zinc ions. In the present study, the AF properties of the MTs in the Asian tiger mosquito, Aedes albopictus, were characterized in different stages of the insect’s life cycle, tryptophan-administered larvae and blood-fed adult females. Confocal imaging and microspectroscopy showed AF changes in the distribution of intracellular, brilliant granules and in the emission spectral shape and amplitude between the proximal and distal segments of MTs across the different samples. The findings suggest AF can serve as a promising marker for investigating the functional status of MTs in response to metabolic alterations, contributing to the use of MTs as a potential research model in biomedicine.
Fil: Croce, Anna Cleta. Consiglio Nazionale delle Ricerche; Italia
Fil: Garbelli, Anna. Consiglio Nazionale delle Ricerche; Italia
Fil: Moyano, Andrea del Huerto. Consiglio Nazionale delle Ricerche; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Soldano, Sara. Universita degli Studi di Pavia; Italia
Fil: Tejeda Guzmán, Carlos. No especifíca;
Fil: Missirlis, Fanis. No especifíca;
Fil: Scolari, Francesca. Universita degli Studi di Pavia; Italia
Materia
mosquito developmental stages
fluorescent granules
dietary intake
tryptophan
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/255839
Acceder
id CONICETDig_96d363afeafd07e6f25659617ad0d9dd
oai_identifier_str oai:ri.conicet.gov.ar:11336/255839
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictusCroce, Anna CletaGarbelli, AnnaMoyano, Andrea del HuertoSoldano, SaraTejeda Guzmán, CarlosMissirlis, FanisScolari, Francescamosquito developmental stagesfluorescent granulesdietary intaketryptophanhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Malpighian tubules (MTs) are arthropod excretory organs crucial for the osmoregulation, detoxification and excretion of xenobiotics and metabolic wastes, which include tryptophan degradation products along the kynurenine (KYN) pathway. Specifically, the toxic intermediate 3-hydroxy kynurenine (3-HK) is metabolized through transamination to xanthurenic acid or in the synthesis of ommochrome pigments. Early investigations in Drosophila larval fat bodies revealed an intracellular autofluorescence (AF) that depended on tryptophan administration. Subsequent observations documented AF changes in the MTs of Drosophila eye-color mutants genetically affecting the conversion of tryptophan to KYN or 3-HK and the intracellular availability of zinc ions. In the present study, the AF properties of the MTs in the Asian tiger mosquito, Aedes albopictus, were characterized in different stages of the insect’s life cycle, tryptophan-administered larvae and blood-fed adult females. Confocal imaging and microspectroscopy showed AF changes in the distribution of intracellular, brilliant granules and in the emission spectral shape and amplitude between the proximal and distal segments of MTs across the different samples. The findings suggest AF can serve as a promising marker for investigating the functional status of MTs in response to metabolic alterations, contributing to the use of MTs as a potential research model in biomedicine.Fil: Croce, Anna Cleta. Consiglio Nazionale delle Ricerche; ItaliaFil: Garbelli, Anna. Consiglio Nazionale delle Ricerche; ItaliaFil: Moyano, Andrea del Huerto. Consiglio Nazionale delle Ricerche; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Soldano, Sara. Universita degli Studi di Pavia; ItaliaFil: Tejeda Guzmán, Carlos. No especifíca;Fil: Missirlis, Fanis. No especifíca;Fil: Scolari, Francesca. Universita degli Studi di Pavia; ItaliaMolecular Diversity Preservation International2023-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/255839Croce, Anna Cleta; Garbelli, Anna; Moyano, Andrea del Huerto; Soldano, Sara; Tejeda Guzmán, Carlos; et al.; Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 25; 1; 12-2023; 1-191422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/25/1/245info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms25010245info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:23:50Zoai:ri.conicet.gov.ar:11336/255839instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:23:50.621CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus
title Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus
spellingShingle Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus
Croce, Anna Cleta
mosquito developmental stages
fluorescent granules
dietary intake
tryptophan
title_short Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus
title_full Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus
title_fullStr Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus
title_full_unstemmed Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus
title_sort Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus
dc.creator.none.fl_str_mv Croce, Anna Cleta
Garbelli, Anna
Moyano, Andrea del Huerto
Soldano, Sara
Tejeda Guzmán, Carlos
Missirlis, Fanis
Scolari, Francesca
author Croce, Anna Cleta
author_facet Croce, Anna Cleta
Garbelli, Anna
Moyano, Andrea del Huerto
Soldano, Sara
Tejeda Guzmán, Carlos
Missirlis, Fanis
Scolari, Francesca
author_role author
author2 Garbelli, Anna
Moyano, Andrea del Huerto
Soldano, Sara
Tejeda Guzmán, Carlos
Missirlis, Fanis
Scolari, Francesca
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv mosquito developmental stages
fluorescent granules
dietary intake
tryptophan
topic mosquito developmental stages
fluorescent granules
dietary intake
tryptophan
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Malpighian tubules (MTs) are arthropod excretory organs crucial for the osmoregulation, detoxification and excretion of xenobiotics and metabolic wastes, which include tryptophan degradation products along the kynurenine (KYN) pathway. Specifically, the toxic intermediate 3-hydroxy kynurenine (3-HK) is metabolized through transamination to xanthurenic acid or in the synthesis of ommochrome pigments. Early investigations in Drosophila larval fat bodies revealed an intracellular autofluorescence (AF) that depended on tryptophan administration. Subsequent observations documented AF changes in the MTs of Drosophila eye-color mutants genetically affecting the conversion of tryptophan to KYN or 3-HK and the intracellular availability of zinc ions. In the present study, the AF properties of the MTs in the Asian tiger mosquito, Aedes albopictus, were characterized in different stages of the insect’s life cycle, tryptophan-administered larvae and blood-fed adult females. Confocal imaging and microspectroscopy showed AF changes in the distribution of intracellular, brilliant granules and in the emission spectral shape and amplitude between the proximal and distal segments of MTs across the different samples. The findings suggest AF can serve as a promising marker for investigating the functional status of MTs in response to metabolic alterations, contributing to the use of MTs as a potential research model in biomedicine.
Fil: Croce, Anna Cleta. Consiglio Nazionale delle Ricerche; Italia
Fil: Garbelli, Anna. Consiglio Nazionale delle Ricerche; Italia
Fil: Moyano, Andrea del Huerto. Consiglio Nazionale delle Ricerche; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Soldano, Sara. Universita degli Studi di Pavia; Italia
Fil: Tejeda Guzmán, Carlos. No especifíca;
Fil: Missirlis, Fanis. No especifíca;
Fil: Scolari, Francesca. Universita degli Studi di Pavia; Italia
description Malpighian tubules (MTs) are arthropod excretory organs crucial for the osmoregulation, detoxification and excretion of xenobiotics and metabolic wastes, which include tryptophan degradation products along the kynurenine (KYN) pathway. Specifically, the toxic intermediate 3-hydroxy kynurenine (3-HK) is metabolized through transamination to xanthurenic acid or in the synthesis of ommochrome pigments. Early investigations in Drosophila larval fat bodies revealed an intracellular autofluorescence (AF) that depended on tryptophan administration. Subsequent observations documented AF changes in the MTs of Drosophila eye-color mutants genetically affecting the conversion of tryptophan to KYN or 3-HK and the intracellular availability of zinc ions. In the present study, the AF properties of the MTs in the Asian tiger mosquito, Aedes albopictus, were characterized in different stages of the insect’s life cycle, tryptophan-administered larvae and blood-fed adult females. Confocal imaging and microspectroscopy showed AF changes in the distribution of intracellular, brilliant granules and in the emission spectral shape and amplitude between the proximal and distal segments of MTs across the different samples. The findings suggest AF can serve as a promising marker for investigating the functional status of MTs in response to metabolic alterations, contributing to the use of MTs as a potential research model in biomedicine.
publishDate 2023
dc.date.none.fl_str_mv 2023-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/255839
Croce, Anna Cleta; Garbelli, Anna; Moyano, Andrea del Huerto; Soldano, Sara; Tejeda Guzmán, Carlos; et al.; Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 25; 1; 12-2023; 1-19
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/255839
identifier_str_mv Croce, Anna Cleta; Garbelli, Anna; Moyano, Andrea del Huerto; Soldano, Sara; Tejeda Guzmán, Carlos; et al.; Developmental and Nutritional Dynamics of Malpighian Tubule Autofluorescence in the Asian Tiger Mosquito Aedes albopictus; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 25; 1; 12-2023; 1-19
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/25/1/245
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms25010245
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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