Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan

Autores
Crupkin, Andrea Carina; Carriquiriborde, Pedro; Mendieta, Julieta Renee; Panzeri, Ana M.; Ballesteros, Maria Laura; Miglioranza, Karina Silvia Beatriz; Menone, Mirta Lujan
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Short-term responses at the antioxidant enzymatic systems, together with genotoxic effects were studied in the freshwater fish Australoheros facetus, exposed to endosulfan (ES) (0.02, 0.5, 5, 10 lg/L) for 24 h. Brain was the most responsive organ, showing inhibition of the enzymatic systems together with an increase of hydrogen peroxide (H2O2) content. Concentration-dependent inhibition was observed for superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) with IC25 values of 0.012, 0.017, 0.018 lg/L, respectively. In liver, a similar behavior was observed for SOD with IC25 values of 2.22 lg/L. In addition, increased thiobarbituric acid reactive substances (TBARs) at 5 lg/L and H2O2 at 5 and 10 lg/L were observed. No effects were evidenced on ethoxyresorufin O-deethylase (EROD), glutathione-S-transferase (GST), GR and CAT activities. In gills, only H2O2 decreased at 0.5 and 5 lg/L ES. Genotoxic effects were detected by the increase of the frequency of both, nuclear abnormalities (NA) at 0.02 lg/L and micronucleus (MN) at 5 lg/L. Environmentally realistic concentrations of ES exerted toxic responses in A. facetus, encouraging the further field validation of the observed pattern (tissue specificity, sensitiveness and concentration-response relationship) as a potential suit of biomarkers for assessing acute sublethal effects in A. facetus under short-term pulsed exposure to ES.
Fil: Crupkin, Andrea Carina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; Argentina
Fil: Carriquiriborde, Pedro. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; Argentina
Fil: Mendieta, Julieta Renee. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Panzeri, Ana M.. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Departamento de Biologia. Laboratorio de Genetica; Argentina
Fil: Ballesteros, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; Argentina
Fil: Miglioranza, Karina Silvia Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; Argentina
Materia
Antioxidant Enzymes
Biomarkers
Endosulfan
Hydrogen Peroxide
Lipid Peroxide
Micronuclei
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7964
Acceder
id CONICETDig_96cf0ebcc91d20532ef06306b76b8238
oai_identifier_str oai:ri.conicet.gov.ar:11336/7964
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfanCrupkin, Andrea CarinaCarriquiriborde, PedroMendieta, Julieta ReneePanzeri, Ana M.Ballesteros, Maria LauraMiglioranza, Karina Silvia BeatrizMenone, Mirta LujanAntioxidant EnzymesBiomarkersEndosulfanHydrogen PeroxideLipid PeroxideMicronucleihttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Short-term responses at the antioxidant enzymatic systems, together with genotoxic effects were studied in the freshwater fish Australoheros facetus, exposed to endosulfan (ES) (0.02, 0.5, 5, 10 lg/L) for 24 h. Brain was the most responsive organ, showing inhibition of the enzymatic systems together with an increase of hydrogen peroxide (H2O2) content. Concentration-dependent inhibition was observed for superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) with IC25 values of 0.012, 0.017, 0.018 lg/L, respectively. In liver, a similar behavior was observed for SOD with IC25 values of 2.22 lg/L. In addition, increased thiobarbituric acid reactive substances (TBARs) at 5 lg/L and H2O2 at 5 and 10 lg/L were observed. No effects were evidenced on ethoxyresorufin O-deethylase (EROD), glutathione-S-transferase (GST), GR and CAT activities. In gills, only H2O2 decreased at 0.5 and 5 lg/L ES. Genotoxic effects were detected by the increase of the frequency of both, nuclear abnormalities (NA) at 0.02 lg/L and micronucleus (MN) at 5 lg/L. Environmentally realistic concentrations of ES exerted toxic responses in A. facetus, encouraging the further field validation of the observed pattern (tissue specificity, sensitiveness and concentration-response relationship) as a potential suit of biomarkers for assessing acute sublethal effects in A. facetus under short-term pulsed exposure to ES.Fil: Crupkin, Andrea Carina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; ArgentinaFil: Carriquiriborde, Pedro. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; ArgentinaFil: Mendieta, Julieta Renee. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Panzeri, Ana M.. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Departamento de Biologia. Laboratorio de Genetica; ArgentinaFil: Ballesteros, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; ArgentinaFil: Miglioranza, Karina Silvia Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; ArgentinaAcademic Press Inc Elsevier Science2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7964Crupkin, Andrea Carina; Carriquiriborde, Pedro; Mendieta, Julieta Renee; Panzeri, Ana M.; Ballesteros, Maria Laura; et al.; Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan; Academic Press Inc Elsevier Science; Pesticide Biochemistry And Physiology; 105; 2; 1-2013; 102-1100048-3575enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0048357512001861info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pestbp.2012.12.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:49Zoai:ri.conicet.gov.ar:11336/7964instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:49.939CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan
title Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan
spellingShingle Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan
Crupkin, Andrea Carina
Antioxidant Enzymes
Biomarkers
Endosulfan
Hydrogen Peroxide
Lipid Peroxide
Micronuclei
title_short Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan
title_full Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan
title_fullStr Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan
title_full_unstemmed Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan
title_sort Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan
dc.creator.none.fl_str_mv Crupkin, Andrea Carina
Carriquiriborde, Pedro
Mendieta, Julieta Renee
Panzeri, Ana M.
Ballesteros, Maria Laura
Miglioranza, Karina Silvia Beatriz
Menone, Mirta Lujan
author Crupkin, Andrea Carina
author_facet Crupkin, Andrea Carina
Carriquiriborde, Pedro
Mendieta, Julieta Renee
Panzeri, Ana M.
Ballesteros, Maria Laura
Miglioranza, Karina Silvia Beatriz
Menone, Mirta Lujan
author_role author
author2 Carriquiriborde, Pedro
Mendieta, Julieta Renee
Panzeri, Ana M.
Ballesteros, Maria Laura
Miglioranza, Karina Silvia Beatriz
Menone, Mirta Lujan
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Antioxidant Enzymes
Biomarkers
Endosulfan
Hydrogen Peroxide
Lipid Peroxide
Micronuclei
topic Antioxidant Enzymes
Biomarkers
Endosulfan
Hydrogen Peroxide
Lipid Peroxide
Micronuclei
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Short-term responses at the antioxidant enzymatic systems, together with genotoxic effects were studied in the freshwater fish Australoheros facetus, exposed to endosulfan (ES) (0.02, 0.5, 5, 10 lg/L) for 24 h. Brain was the most responsive organ, showing inhibition of the enzymatic systems together with an increase of hydrogen peroxide (H2O2) content. Concentration-dependent inhibition was observed for superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) with IC25 values of 0.012, 0.017, 0.018 lg/L, respectively. In liver, a similar behavior was observed for SOD with IC25 values of 2.22 lg/L. In addition, increased thiobarbituric acid reactive substances (TBARs) at 5 lg/L and H2O2 at 5 and 10 lg/L were observed. No effects were evidenced on ethoxyresorufin O-deethylase (EROD), glutathione-S-transferase (GST), GR and CAT activities. In gills, only H2O2 decreased at 0.5 and 5 lg/L ES. Genotoxic effects were detected by the increase of the frequency of both, nuclear abnormalities (NA) at 0.02 lg/L and micronucleus (MN) at 5 lg/L. Environmentally realistic concentrations of ES exerted toxic responses in A. facetus, encouraging the further field validation of the observed pattern (tissue specificity, sensitiveness and concentration-response relationship) as a potential suit of biomarkers for assessing acute sublethal effects in A. facetus under short-term pulsed exposure to ES.
Fil: Crupkin, Andrea Carina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; Argentina
Fil: Carriquiriborde, Pedro. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; Argentina
Fil: Mendieta, Julieta Renee. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Panzeri, Ana M.. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Departamento de Biologia. Laboratorio de Genetica; Argentina
Fil: Ballesteros, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; Argentina
Fil: Miglioranza, Karina Silvia Beatriz. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; Argentina
description Short-term responses at the antioxidant enzymatic systems, together with genotoxic effects were studied in the freshwater fish Australoheros facetus, exposed to endosulfan (ES) (0.02, 0.5, 5, 10 lg/L) for 24 h. Brain was the most responsive organ, showing inhibition of the enzymatic systems together with an increase of hydrogen peroxide (H2O2) content. Concentration-dependent inhibition was observed for superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) with IC25 values of 0.012, 0.017, 0.018 lg/L, respectively. In liver, a similar behavior was observed for SOD with IC25 values of 2.22 lg/L. In addition, increased thiobarbituric acid reactive substances (TBARs) at 5 lg/L and H2O2 at 5 and 10 lg/L were observed. No effects were evidenced on ethoxyresorufin O-deethylase (EROD), glutathione-S-transferase (GST), GR and CAT activities. In gills, only H2O2 decreased at 0.5 and 5 lg/L ES. Genotoxic effects were detected by the increase of the frequency of both, nuclear abnormalities (NA) at 0.02 lg/L and micronucleus (MN) at 5 lg/L. Environmentally realistic concentrations of ES exerted toxic responses in A. facetus, encouraging the further field validation of the observed pattern (tissue specificity, sensitiveness and concentration-response relationship) as a potential suit of biomarkers for assessing acute sublethal effects in A. facetus under short-term pulsed exposure to ES.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7964
Crupkin, Andrea Carina; Carriquiriborde, Pedro; Mendieta, Julieta Renee; Panzeri, Ana M.; Ballesteros, Maria Laura; et al.; Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan; Academic Press Inc Elsevier Science; Pesticide Biochemistry And Physiology; 105; 2; 1-2013; 102-110
0048-3575
url http://hdl.handle.net/11336/7964
identifier_str_mv Crupkin, Andrea Carina; Carriquiriborde, Pedro; Mendieta, Julieta Renee; Panzeri, Ana M.; Ballesteros, Maria Laura; et al.; Oxidative stress and genotoxicity in the South American Cichlid, Australoheros facetus, after short-term sublethal exposure to endosulfan; Academic Press Inc Elsevier Science; Pesticide Biochemistry And Physiology; 105; 2; 1-2013; 102-110
0048-3575
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0048357512001861
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pestbp.2012.12.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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