Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease

Autores
Rodríguez, Fernanda Mariel; Salvetti, Natalia Raquel; Colombero, M.; Stangaferro, M.; Barbeito, Claudio Gustavo; Ortega, Hugo Hector; Rey, Florencia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator. The aim of the present study was to determine whether the altered levels in IGF1 detected in bovines with COD are associated with changes at mRNA level or with differential modulation by IGFBPs. The mRNA levels of the IGF components studied were analyzed by real time PCR and in situ hybridization, and IGFBP expression and activity were assayed by immunohistochemistry and ligand blot, respectively. Results showed a decreased IGF1 mRNA level due to a lower granulosa cell gene expression in cystic follicles (P menor 0.05). Results also showed variations in IGFBP expression in the intraovarian cellular compartment and concentration in follicular fluid, and suggest that IGFBP3 is a key regulator of intrafollicular IGF1 in animals with COD.
Fil: Rodríguez, Fernanda Mariel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Colombero, M.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina
Fil: Stangaferro, M.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Materia
Cattle
Cystic ovarian disease
Insulin-like growth factor
Ovaries
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6398
Acceder
id CONICETDig_9684ab451ea7e201a33b2e3b957d624f
oai_identifier_str oai:ri.conicet.gov.ar:11336/6398
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interaction between IGF1 and IGFBPs in bovine cystic ovarian diseaseRodríguez, Fernanda MarielSalvetti, Natalia RaquelColombero, M.Stangaferro, M.Barbeito, Claudio GustavoOrtega, Hugo HectorRey, FlorenciaCattleCystic ovarian diseaseInsulin-like growth factorOvarieshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator. The aim of the present study was to determine whether the altered levels in IGF1 detected in bovines with COD are associated with changes at mRNA level or with differential modulation by IGFBPs. The mRNA levels of the IGF components studied were analyzed by real time PCR and in situ hybridization, and IGFBP expression and activity were assayed by immunohistochemistry and ligand blot, respectively. Results showed a decreased IGF1 mRNA level due to a lower granulosa cell gene expression in cystic follicles (P menor 0.05). Results also showed variations in IGFBP expression in the intraovarian cellular compartment and concentration in follicular fluid, and suggest that IGFBP3 is a key regulator of intrafollicular IGF1 in animals with COD.Fil: Rodríguez, Fernanda Mariel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Colombero, M.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; ArgentinaFil: Stangaferro, M.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; ArgentinaElsevier2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6398Rodríguez, Fernanda Mariel; Salvetti, Natalia Raquel; Colombero, M.; Stangaferro, M.; Barbeito, Claudio Gustavo; et al.; Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease; Elsevier; Animal Reproduction Science; 140; 1-2; 5-2013; 14-250378-4320enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378432013001449info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2013.04.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:32Zoai:ri.conicet.gov.ar:11336/6398instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:32.358CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
title Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
spellingShingle Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
Rodríguez, Fernanda Mariel
Cattle
Cystic ovarian disease
Insulin-like growth factor
Ovaries
title_short Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
title_full Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
title_fullStr Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
title_full_unstemmed Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
title_sort Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
dc.creator.none.fl_str_mv Rodríguez, Fernanda Mariel
Salvetti, Natalia Raquel
Colombero, M.
Stangaferro, M.
Barbeito, Claudio Gustavo
Ortega, Hugo Hector
Rey, Florencia
author Rodríguez, Fernanda Mariel
author_facet Rodríguez, Fernanda Mariel
Salvetti, Natalia Raquel
Colombero, M.
Stangaferro, M.
Barbeito, Claudio Gustavo
Ortega, Hugo Hector
Rey, Florencia
author_role author
author2 Salvetti, Natalia Raquel
Colombero, M.
Stangaferro, M.
Barbeito, Claudio Gustavo
Ortega, Hugo Hector
Rey, Florencia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cattle
Cystic ovarian disease
Insulin-like growth factor
Ovaries
topic Cattle
Cystic ovarian disease
Insulin-like growth factor
Ovaries
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator. The aim of the present study was to determine whether the altered levels in IGF1 detected in bovines with COD are associated with changes at mRNA level or with differential modulation by IGFBPs. The mRNA levels of the IGF components studied were analyzed by real time PCR and in situ hybridization, and IGFBP expression and activity were assayed by immunohistochemistry and ligand blot, respectively. Results showed a decreased IGF1 mRNA level due to a lower granulosa cell gene expression in cystic follicles (P menor 0.05). Results also showed variations in IGFBP expression in the intraovarian cellular compartment and concentration in follicular fluid, and suggest that IGFBP3 is a key regulator of intrafollicular IGF1 in animals with COD.
Fil: Rodríguez, Fernanda Mariel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Colombero, M.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina
Fil: Stangaferro, M.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfologicas. Laboratorio de Biologia Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Ciencias Veterinarias del Litoral; Argentina
description Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator. The aim of the present study was to determine whether the altered levels in IGF1 detected in bovines with COD are associated with changes at mRNA level or with differential modulation by IGFBPs. The mRNA levels of the IGF components studied were analyzed by real time PCR and in situ hybridization, and IGFBP expression and activity were assayed by immunohistochemistry and ligand blot, respectively. Results showed a decreased IGF1 mRNA level due to a lower granulosa cell gene expression in cystic follicles (P menor 0.05). Results also showed variations in IGFBP expression in the intraovarian cellular compartment and concentration in follicular fluid, and suggest that IGFBP3 is a key regulator of intrafollicular IGF1 in animals with COD.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6398
Rodríguez, Fernanda Mariel; Salvetti, Natalia Raquel; Colombero, M.; Stangaferro, M.; Barbeito, Claudio Gustavo; et al.; Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease; Elsevier; Animal Reproduction Science; 140; 1-2; 5-2013; 14-25
0378-4320
url http://hdl.handle.net/11336/6398
identifier_str_mv Rodríguez, Fernanda Mariel; Salvetti, Natalia Raquel; Colombero, M.; Stangaferro, M.; Barbeito, Claudio Gustavo; et al.; Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease; Elsevier; Animal Reproduction Science; 140; 1-2; 5-2013; 14-25
0378-4320
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0378432013001449
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2013.04.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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